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The effect of lignosulfonate chemical stimulator to the metabolites of Euglena sp. Luthfiana, Dwi Hardianti; Karilanata, Khalid Erlangga; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Amelia, Ria; Erfianti, Tia; Larasati, Ersi; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.30804

Abstract

Euglena sp. contains several metabolites that play a role in various sectors, likely biofuels, food, feed, and pharmaceuticals. Multiple treatments are required to increase the content of Euglena sp.to support its use. In this study, a chemical stimulant in the form of lignosulfonate was added to Euglena sp. in order to increase its natural potency as measured by lipid, carbohydrate, protein, paramylon content, and biomass productivity. Lignosulfonate is a macromolecular polyelectrolyte and anionic surfactant that is unique and multifunctional. This lignosulfonate can act as an emulsifier, dispersing agent, binder, and stabilizer of lipids in Euglena sp. cells. In addition to the lignosulfonate chemical stimulator, it can affect the growth process of Euglena sp. so that it can produce good-quality cells. The method used was a Completely Randomized Factorial Design (CRD) with three treatments and three replications. The results obtained indicated that the addition of lignosulfonate was significantly changed the cell and protein density tests. Therefore, the optimal concentration of lignosulfonate can be used as an agent to increase the growth rate and metabolites of Euglena sp. significantly.Keywords:BiofuelEuglena sp.Growth rateslignosulfonateMetabolites
The effect of lignosulfonate chemical stimulator to the metabolites of Euglena sp. Luthfiana, Dwi Hardianti; Karilanata, Khalid Erlangga; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Amelia, Ria; Erfianti, Tia; Larasati, Ersi; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.30804

Abstract

Euglena sp. contains several metabolites that play a role in various sectors, likely biofuels, food, feed, and pharmaceuticals. Multiple treatments are required to increase the content of Euglena sp.to support its use. In this study, a chemical stimulant in the form of lignosulfonate was added to Euglena sp. in order to increase its natural potency as measured by lipid, carbohydrate, protein, paramylon content, and biomass productivity. Lignosulfonate is a macromolecular polyelectrolyte and anionic surfactant that is unique and multifunctional. This lignosulfonate can act as an emulsifier, dispersing agent, binder, and stabilizer of lipids in Euglena sp. cells. In addition to the lignosulfonate chemical stimulator, it can affect the growth process of Euglena sp. so that it can produce good-quality cells. The method used was a Completely Randomized Factorial Design (CRD) with three treatments and three replications. The results obtained indicated that the addition of lignosulfonate was significantly changed the cell and protein density tests. Therefore, the optimal concentration of lignosulfonate can be used as an agent to increase the growth rate and metabolites of Euglena sp. significantly.Keywords:BiofuelEuglena sp.Growth rateslignosulfonateMetabolites
Silica's Medium in Various Concentration Effect on Navicula sp. Metabolism Karillanata, Khalid Erlangga; Luthfiana, Dwi Hardianti; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Amelia, Ria; Erfianti, Tia; Suyono, Eko Agus
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.31078

Abstract

 Abstract Navicula sp., an aquatic microalgae species, are numerous and diverse, with high metabolites so they hold great potential in biotechnology. Although it has many advantages, it is often not used in industry. In silica medium, Navicula sp. produces various metabolites depending on their concentration. This research aims to discover how adding silica to the medium affects the growth and production of Navicula sp. metabolites so that cultivation can be carried out at low cost and with maximum results. For 21 days, this experiment was carried out with three concentrations of silica (1; 1.5; and 2 mL/L) and one control (0 mL/L) grown each in a 500 mL culture of Navicula sp. Repetition was done thrice for each measurement parameter; growth speed, biomass production, lipids, carbohydrates, and protein. Medium silica 1.5 mL/L was the optimal concentration for growth speed, biomass production, and carbohydrate production for Navicula sp. (0.083; 0.54; and 0.075 mg/day, respectively). Meanwhile, the optimal silica concentration for lipid and protein production for Navicula sp. were 1 mL/L medium and control medium (0.517 and 0.8 × 10-2 mg/day, respectively). Overall, this research can be used to grow Navicula sp. in producing specific metabolites optimally. AbstrakNavicula sp., spesies mikroalga akuatik, sangat banyak dan beragam dengan metabolit yang tinggi, sehingga memiliki potensi yang besar dalam bioteknologi. Meskipun memiliki banyak keunggulan, spesies ini seringkali tidak digunakan dalam industri. Dalam medium silika, Navicula sp. menghasilkan berbagai metabolit tergantung pada konsentrasinya. Tujuan dari penelitan ini adalah untuk mengetahui bagaimana penambahan silika dalam medium memengaruhi pertumbuhan dan produksi metabolit Navicula sp., sehingga kultivasi dapat dilakukan dengan biaya yang rendah dan hasil yang maksimal. Selama 21 hari, eksperimen ini dilakukan dengan tiga konsentrasi silika (1; 1,5; dan 2 mL/L) dan satu kontrol (0 mL/L) yang ditumbuhkan di dalam 500 mL kultur Navicula sp. Pengulangan dilakukan tiga kali untuk setiap parameter pengukuran, yaitu kecepatan pertumbuhan, produksi biomassa, lipid, karbohidrat, dan protein. Medium silika 1,5 mL/L merupakan konsentrasi yang optimal untuk kecepatan pertumbuhan, produksi biomassa, dan produksi karbohidrat bagi Navicula sp. (0.083; 0.54;  dan 0.075 mg/hari, secara berurutan). Sementara itu, konsentrasi silika yang optimal untuk produksi lipid dan protein bagi Navicula sp., secara berurutan, adalah medium 1 mL/L dan medium kontrol (0.517 dan 0.8 × 10-2 mg/hari). Secara keseluruhan, penelitian ini dapat dijadikan sebagai solusi untuk menumbuhkan Navicula sp. dalam memproduksi metabolit tertentu secara optimal.