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Analisa Bakteri Coliform pada Depot Air Minum Isi Ulang yang Diperjualbelikan di Jalan Benteng Hilir Tembung Medan Silfia, Marti; Sembiring, Terang Uli. J
Journal Scientific of Mandalika (JSM) e-ISSN 2745-5955 | p-ISSN 2809-0543 Vol. 6 No. 8 (2025)
Publisher : Institut Penelitian dan Pengembangan Mandalika Indonesia (IP2MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/10.36312/vol6iss8pp2298-3306

Abstract

Drinking water is a primary need for human life, but its quality must meet health standards to be safe for consumption. Refillable Drinking Water Depot (DAMIU) is an alternative for the community in fulfilling drinking water needs at a more affordable price. However, not all refill drinking water meets the health standards set by the Regulation of the Minister of Health of the Republic of Indonesia No. 492/MENKES/PER/IV/2010. This study aims to detect the presence of Coliform bacteria in refill drinking water sold in Jalan Benteng Hilir Tembung, Medan. The method used in this research is descriptive method with microbiological test approach using Most Probable Number (MPN). The examination was conducted at the Microbiology Laboratory of Sari Mutiara Indonesia University Medan using Lactose Broth and Brilliant Green Lactose Bile (BGLB) broth media. The samples taken were 6 samples from various refill drinking water depots in the research location. The results showed that of the 6 samples tested, 4 samples showed positive results for the presence of Coliform bacteria based on the initial test with Lactose Broth media. Furthermore, confirmation tests with BGLB media at 37°C for 1x24 hours showed MPN numbers ranging from 8.8 to 240/100 ml samples, while at 44°C for 1x24 hours, MPN numbers ranged from 10 to 27/100 ml samples. These results indicate that most of the tested refill drinking water does not meet the quality standards set by the government. The conclusion of this study is that there is Coliform bacterial contamination in some of the refill drinking water samples tested, so the water is not suitable for consumption without further treatment. Therefore, strict supervision of the quality of refill drinking water is needed as well as education to the public about the importance of consuming water that meets health standards to prevent the risk of disease due to bacterial contamination.
Gambaran Jumlah Sel Monosit dan Sel Limfosit Pada Penderita Tuberkulosis Paru di UPTD Rumah Sakit Khusus Paru Provinsi Sumatera Utara Sembiring, Terang U; Silfia, Marti; Erlan Aritonan; Denrison Purba
JURNAL TEKNOLOGI KESEHATAN DAN ILMU SOSIAL (TEKESNOS) Vol. 6 No. 2 (2024): JURNAL TEKNOLOGI, KESEHATAN DAN ILMU SOSIAL (TEKESNOS)
Publisher : Universitas Sari Mutiara Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pulmonary TB is a chronic infection when associated with M.TB germs. Most mycobacterium tuberculosis germs infect the lungs, this triggers the cause of pulmonary tuberculosis, but these bacteria can infect other parts of the body. Transmission of tuberculosis can be caused by AFB Positive sufferers, AFB Negative sufferers and infections by inhaling air containing sputum microscopically. Tuberculosis is the main cause of monocytosis and lymphopenia. Monocytes are the body's immune response to detect tuberculosis in the form of cellular reactions and formation of the immune response is greatly influenced by the beginning. the body to tuberculosis in cellular reactions and as the main cells forming tubercles, monocytosis is an increase in the number above 900 / mm³. A decrease in the number of lymphocytes indicates an active tuberculosis process; Lymphocytes play an important role in the adaptive immune system against mycobacterium tuberculosis. A decrease in the number of lymphocytes is called lymphopenia below 2000/µL. The purpose of this study was to determine how many monocytes and lymphocytes were found in individuals suffering from pulmonary tuberculosis. Primary and secondary data were used to describe the study design. The laboratory examination method that uses this methodology is automatic, namely the Cell-dyn Emerald 22 hematology analyzer. From the results of the study at the UPTD Special Lung Hospital of North Sumatra Province, 20 samples examined contained 11 samples in a state of monocytosis (55%), 3 samples experienced monocytopenia (15%), and 6 samples with normal monocytes (30%). In lymphocytes there were 2 samples in a state of lymphocytosis (10%), 13 samples in a state of lymphopenia (65%) and 5 samples of normal lymphocytes (25%). An increase in monocytes indicates the activity of tubercle formation in the blood as a sign of active spread of tuberculosis and a decrease in lymphocytes indicates an increased risk of bacterial growth, and the body's ability to fight infection is hampered, resulting in permanent damage and causing lung complications.