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All Journal HAYATI Journal of Biosciences Jurnal Penelitian Pendidikan IPA (JPPIPA)
Wijaya, Hans
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Materials Science & Nanotechnology Physics

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Medium Optimization for Recombinant Human Papillomavirus Type 52 L1 Protein Production in Pichia pastoris GS115 Platform on Bioreactor Scale Mustopa, Apon Zaenal; Nur Amani, Febriyanti; Irawan, Herman; Novianti, Ela; Swasthikawati, Sri; Ekawati, Nurlaili; Nurfatwa, Maritsa; Joko Wahyono, Daniel; Juanssilfero, Ario Betha; Mamangkey, Jendri; Purnomo, Yudi; Hertati, Ai; Wijaya, Hans; Dewi, Kartika Sari; Ningrum, Ratih Asmana
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1283-1294

Abstract

Human papillomavirus (HPV) stands as the primary etiological agent in the development of invasive cervical cancer worldwide. The L1 protein is a pivotal constituent of prophylactic HPV vaccines. Notably, HPV type 52 is one of the most prevalent genotypes found in squamous cell carcinoma cases in Indonesia. This research endeavor aims to enhance the productivity of recombinant HPV-52 L1 protein by optimizing the culture conditions of P. pastoris GS115 cells. In this study, we conducted trials employing 17 different media variants to optimize the expression of recombinant HPV-52 L1 protein. The results from small-scale experiments revealed three media, namely SYN6.10, BMMY, and SYN6.1, which exhibited promising yields of recombinant HPV-52 L1 protein as assessed through ELISA or immunoassay analysis. We succeeded in refining the SYN6.10 derivative, denoted as SYN6.10b, specifically designed for use in 1-L and 5-L bioreactors. This achievement was realized by adjusting Trace Element Solution (TES) and Vitamin Solution (VS) concentrations and implementing a methanol fed-batch phase with the addition of 0.3% methanol after 24 and 48 hours of fermentation in the P. pastoris medium. Further visualizations through SDS-PAGE and western blot analysis confirmed the protein after 72 hours of fermentation in a 1-L bioreactor using the SYN6.10b medium. In conclusion, the SYN6.10b medium required a 72 hours fermentation period to successfully express recombinant HPV-52 L1 protein in the P. pastoris platform.
Bahasa Inggris Erawati, Suci; Idamawati , Idamawati; Wijaya, Hans
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.6348

Abstract

Halitosis, known as bad breath, is a condition in which many patients complain about that cause unpleasant odor that comes from the mouth, which can affect the quality of life both psychologically and social life. Knowledge of maintaining oral health plays an important role in oral health conditions. This research to discover whether there is correlation between halitosis and the level of oral hygiene in high school students at Letjen S.Parman school in Medan. Researcher used a descriptive observational method which then analyzed the data using SPSS bivariate analysis with Pearson measuring to analyze the correlation between halitosis and oral hygiene. Shapiro-Wilk test found sig.value amounting to 0.180 on the questionnaire score and 0.068 on the OHIS, both results are greater than 0.05 indicates the datas are normally distributed. Then bivariate analysis was carried out and the Pearson results obtained showed Sig (2 tailed) value of 0.421, where the significance value was Sig.(2 tailed) 0.004<0.05, so can be affirmed that there is a relation between halitosis and the level of oral hygiene. H1 is accepted, which defines that there is an association between halitosis and the level oral hygiene of high school teenagers at Letjen S. Parman Medan school.
Co-Authors Ai Hertati, Ai Apon Zaenal Mustopa Ario Betha Juanssilfero Ekawati, Nurlaili Erawati, Suci Idamawati , Idamawati Irawan, Herman Jendri Mamangkey Joko Wahyono, Daniel Kartika Sari Dewi Novianti, Ela Nur Amani, Febriyanti Nurfatwa, Maritsa RATIH ASMANA NINGRUM Sri Swasthikawati, Sri Yudi Purnomo