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All Journal HAYATI Journal of Biosciences Majalah Kedokteran Bandung Makara Journal of Health Research Annales Bogorienses
Nurfatwa, Maritsa
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Medicine & Pharmacology

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Aktivitas Makrofag Meningkat Pada Aorta Tikus Hiperkolesterolemia Kurniati, Neng Fisheri; Nurfatwa, Maritsa; Artarini, Aluicia Anita
Majalah Kedokteran Bandung Vol 50, No 1 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.73 KB) | DOI: 10.15395/mkb.v50n1.1159

Abstract

Aterosklerosis merupakan, kondisi inflamasi kronik, faktor resiko penyakit kardiovaskular disebabkan oleh tingginya kadar kolesterol. Tujuan penelitian ini mengevaluasi peran mieloperoksidase (MPO) dan makrofag di aorta dan jantung tikus yang diinduksi hiperkolesterolemia. Penelitian ini dilakukan pada Maret–Agustus 2016 di Laboratorium Farmakologi dan Bioteknologi Institut Teknologi Bandung. Tikus dikelompokkan menjadi kelompok normal dan hiperkolesterolemia. Induksi hiperkolesterolemia dilakukan dengan pemberian pakan tinggi kolesterol, kolesterol murni, asam kolat dan propiltiourasil (KKT) selama 5 bulan. Kolesterol total diukur sebelum induksi, pertengahan, dan setelah induksi. HDL, trigliserida (TG), LDL, indeks aterogenik (IA), jumlah sel darah merah dan sel darah putih diukur setelah induksi. Deteksi ekspresi mieloperoksidase (MPO) dan CD68 pada aorta dan jantung dilakukan dengan metode dot blot dan ELISA. Induksi hiperkolesterolemia selama 5 bulan menghasilkan kadar kolesterol total (364,10±148,46 mg/dL), HDL (7,90±1,29 mg/dL), LDL (307,47±116,91 mg/dL), dan Indeks Aterogenik (1,04±0,23). Kadar kolesterol yang tinggi meningkatkan jumlah sel darah putih yang bersirkulasi namun tidak mempengaruhi jumlah sel darah merah. Jumlah makrofag yang berada di jaringan aorta dan jantung kelompok hiperkolesterolemia meningkat secara signifikan dibanding dengan kelompok normal. Namun, peningkatan aktivitas makrofag yang diukur dari ekspresi MPO hanya teramati pada aorta hewan hiperkolesterolemia, tidak pada jantung. Simpulan, aktivitas makrofag meningkat hanya pada aorta hewan hiperkolesterolemia diduga berperan dalam pembentukan plak ateroma di aorta. Kata kunci: Aorta, CD68, hiperkolesterolemia, makrofag, mieloperoksidase Macrophage Activity Increases in Hypercholesterolemia Rat AortaAtherosclerosis, which is an inflammatory chronic condition, is one of the major risk factors of cardiovascular disease caused by hypercholesterolemia. This study aimed to evaluate role of myeloperoxidase (MPO) and macrophage in aorta and heart of rat hypercholesterolemia. This research was conducted in March–August 2016 at Pharmacology and Biotechnology Laboratory of Institut Teknologi Bandung. Rats were divided into normal and hypercholesterolemia groups. Hypercholesterolemia was induced by cholesterol feeding and CCT (cholesterol, cholic acid and propiltiourasil) oral administration for 5 months. Total cholesterol was measured before induction (T0), in the middle (T2.5), and after induction (T5). HDL, triglyceride (TG), LDL, aterogenic index (IA), red blood, and white blood cell count was measured after induction (T5). Success of induction was proven by the elevation of cholesterol total value of hypercholesterolemia group compared to normal group. Myeloperoxidase (MPO) and CD68 in aorta and heart hypercholesterolemia rat was detected by dot blot and ELISA method. Hypercholesterolemia group showed significant differences in total cholesterol value (364.10±148.46 mg/dL), HDL value (7.90±1.29 mg/dL ), LDL value (307.47±116.91) and Atherogenic Index (1.04±0.23). High level of cholesterol increases circulating white blood cells but have no effect on  circulating red blood cells. Macrophage in the  hypercholesterolemia group increased significantly compared to the normal group. However, the increase in macrophage activity identified throughMPO expression was only seen in hypercholesterolemic aorta but not  in the heart. It is concluded that macrophage activities increase in the aortic tissue, but  not in the heart tissue of the hypercholesterolemia group, which may contribute to the formation of atheroma plaque in aorta. Key words: Aorta, CD68, hypercholesterolemia, macrophage, myeloperoxidase
Myocardial Infarction Elevates Inflammation and Contributes to the Formation of Atheroma Plaques in the Aorta of Hypercholesterolaemic Rats Kurniati, Neng F; Angelia, Teresa; Artarini, Aluicia A; Nurfatwa, Maritsa
Makara Journal of Health Research Vol. 22, No. 1
Publisher : UI Scholars Hub

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Abstract

Background: Myocardial infarction (MI) is the clinical manifestation of coronary heart disease that can be caused by athesclerotic plaque rupture. However, the role of MI in influencing endothelial cells is still not clearly known, especially in atheroma plaque development. The aim of this study was to determine the effect of MI in the inflammatory processes occurring in the hypercholesterolaemic rat aorta and heart by measuring myeloperoxidase (MPO) levels. Methods: Wistar rats were categorised into normal, normal–MI, hypercholesterolaemic and hypercholesterolaemic–MI groups. Hypercholesterolaemia was induced in rats by feeding them with a high-cholesterol diet, followed by oral administrations of cholesterol, cholic acid and propylthiouracil. The MI rat model was created by injecting isoproterenol (intraperitoneal) 1 day before the animals were sacrificed. The success of the induction was confirmed based on a significant increase in total cholesterol values compared to those in the normal group. The inflammatory condition was determined by measuring the MPO levels using the dot blot method. Results: MPO expression was increased significantly in the hypercholesterolaemic rats compared to that in the normal group. The highest aorta MPO expression was observed in the hypercholesterolaemic–MI group. Both MI rats and hypercholesterolaemic rats showed a similar increase in MPO expression in the heart (71.7% and 75.5%, respectively). However, the hypercholesterolaemic–MI rats showed the highest MPO expression (119.59%). Conclusions: MI accelerates inflammation in the aorta of hyper-cholesterolaemic rats.
Truncation on N-Terminal Hydrophobic Domain of L1 Major Capsid Protein of Human Papillomavirus Type 52 Enhances Its Expression in Hansenula polymorpha Arifah, Rosyida Khusniatul; Firdaus, Moh Egy Rahman; Chairunnisa, Sheila; Irawan, Shasmita; Ekawati, Nurlaili; Irawan, Herman; Nurfatwa, Maritsa; Hertati, Ai; Swasthikawati, Sri; Novianti, Ela; Mustafawi, Wike Zahra; Nur Umami, Rifqiyah; Mustopa, Apon Zaenal
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.1062-1072

Abstract

Human papillomavirus (HPV) infection is the main cause of cervical cancer. The administration of the HPV prophylactic vaccine, which is commonly produced based on HPV L1 major capsid protein, significantly reduces the incidence of cervical cancer. However, the coverage of the HPV vaccination program is often hindered due to its relatively high cost. This study aimed to evaluate the impact of N-terminal hydrophobic domain truncation on the expression of L1 major capsid protein of HPV type 52 in Hansenula polymorpha. The truncation enhanced the yield of L1 protein expression compared with the full length, which was confirmed by Western blot and ELISA. Furthermore, the truncated L1 protein formed virus-like particles (VLPs), which were confirmed by transmission electron microscopy (TEM). Bioinformatics analysis showed that the truncated L1 protein was more soluble compared with the full length, possibly increasing the protein expression. These findings could pave the way for the development of a more cost-effective HPV type 52 L1 protein production in H. polymorpha to be used as a VLP-based prophylactic vaccine.
Medium Optimization for Recombinant Human Papillomavirus Type 52 L1 Protein Production in Pichia pastoris GS115 Platform on Bioreactor Scale Mustopa, Apon Zaenal; Nur Amani, Febriyanti; Irawan, Herman; Novianti, Ela; Swasthikawati, Sri; Ekawati, Nurlaili; Nurfatwa, Maritsa; Joko Wahyono, Daniel; Juanssilfero, Ario Betha; Mamangkey, Jendri; Purnomo, Yudi; Hertati, Ai; Wijaya, Hans; Dewi, Kartika Sari; Ningrum, Ratih Asmana
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1283-1294

Abstract

Human papillomavirus (HPV) stands as the primary etiological agent in the development of invasive cervical cancer worldwide. The L1 protein is a pivotal constituent of prophylactic HPV vaccines. Notably, HPV type 52 is one of the most prevalent genotypes found in squamous cell carcinoma cases in Indonesia. This research endeavor aims to enhance the productivity of recombinant HPV-52 L1 protein by optimizing the culture conditions of P. pastoris GS115 cells. In this study, we conducted trials employing 17 different media variants to optimize the expression of recombinant HPV-52 L1 protein. The results from small-scale experiments revealed three media, namely SYN6.10, BMMY, and SYN6.1, which exhibited promising yields of recombinant HPV-52 L1 protein as assessed through ELISA or immunoassay analysis. We succeeded in refining the SYN6.10 derivative, denoted as SYN6.10b, specifically designed for use in 1-L and 5-L bioreactors. This achievement was realized by adjusting Trace Element Solution (TES) and Vitamin Solution (VS) concentrations and implementing a methanol fed-batch phase with the addition of 0.3% methanol after 24 and 48 hours of fermentation in the P. pastoris medium. Further visualizations through SDS-PAGE and western blot analysis confirmed the protein after 72 hours of fermentation in a 1-L bioreactor using the SYN6.10b medium. In conclusion, the SYN6.10b medium required a 72 hours fermentation period to successfully express recombinant HPV-52 L1 protein in the P. pastoris platform.
Antidiabetic, Antioxidants and Antibacterial Activities of Lactic Acid Bacteria (LAB) from Masin (Fermented Sauce from Sumbawa, West Nusa Tenggara, Indonesia) Manguntungi, Baso; Vanggy, Leggina Rezzy; Fidien, Khadijah Alliya Fidien; Saputri, Dinar Suksmayu Saputri; Mustopa, Apon Zaenal; Ekawati, Nurlaili; Nurfatwa, Maritsa; Prastyowati, Anika; Irawan, Shasmita
Annales Bogorienses Vol. 24 No. 1 (2020): Annales Bogorienses
Publisher : BRIN

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Abstract

The study aimed to determine the effectiveness of metabolites from Lactic Acid Bacteria (LAB) derived Masin (fermented sauce from Sumbawa) as antioxidant, antidiabetic, and antibacterial compounds. The LAB isolates were isolated from various strains of Staphylococcus piscifermentan which consisted of Staphylococcus piscifermentans strain CIP103958 (code: 2), strain BULST54 (code: 17), strain SK03 (code: 11), strain ATCC 51136 (code: 34), strain PCM 2409 (code: 28) and strain PU-87 (code: 5). The Metabolites of LAB were analyzed by the bioprospecting test to indicate antidiabetic, antioxidant and antibacterial activities. The isolate (Code: 5) at 500 ug/ml showed the most effective antioxidant activity up to 71%. The isolate (code: 28), at 300 ug/ml revealed to have the most antidiabetic activity up to 43 %. The isolate (code: 2) showed moderate antibacterial activity with the inhibition zone of 5.59 mm. The results of the antidiabetic, antioxidant and antibacterial activity showed that the secondary metabolites produced by LAB from the Masin have broad activities as an antidiabetic, antioxidant and antibacterial.
Preclinical Evaluation of HPV Type 52 L1L2 Chimeric Protein as a Cervical Cancer Vaccine Candidate Sari, Isti Kartika; Pamungkas, Joko; Mustopa, Apon Zaenal; Wibawan, I Wayan Teguh; Mamangkey, Jendri; Chairunnisa, Sheila; Irawan, Herman; Hertati, Ai; Ekawati, Nurlaili; Umami, Rifqiyah Nur; Novianti, Ela; Nurfatwa, Maritsa; Darusman, Huda Shalahudin
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.556-565

Abstract

High-risk human papillomaviruses (HPVs) are the primary etiological agents of cervical cancer, accounting for more than 300,000 deaths annually worldwide. Current prophylactic vaccines based on recombinant L1 major capsid virus-like particles (VLPs) have demonstrated strong efficacy but are restricted to a limited spectrum of HPV types. To address this limitation, the present study evaluated a recombinant L1L2 chimeric protein derived from HPV type 52 as a potential candidate for a broad-spectrum vaccine. The chimeric protein was expressed in Escherichia coli strain BL21 (DE3) and purified for immunization studies. Female BALB/c mice (Mus musculus, n = 5 groups) were immunized, and immune responses were analyzed by enzyme-linked immunosorbent assay (ELISA) and pseudovirion-based neutralization assays (PBNA). The recombinant L1L2 vaccine candidate induced detectable antibody responses against HPV antigens; however, neutralizing activity remained modest. Histopathological analysis of liver and kidney tissues showed no evidence of toxicity, supporting the safety profile of the candidate. In summary, these results suggest that the HPV type 52 L1L2 chimeric protein represents a promising platform for the development of cervical cancer vaccines, although further optimization is required to achieve robust cross-neutralizing efficacy.
Co-Authors Ai Hertati, Ai Angelia, Teresa Anika Prastyowati, Anika Apon Zaenal Mustopa Arifah, Rosyida Khusniatul Ario Betha Juanssilfero Artarini, Aluicia A Artarini, Aluicia Anita Chairunnisa, Sheila Ekawati, Nurlaili Fidien, Khadijah Alliya Fidien Firdaus, Moh Egy Rahman Huda Shalahudin Darusman I wayan Teguh Wibawan Irawan, Herman Irawan, Shasmita Jendri Mamangkey Joko Pamungkas Joko Wahyono, Daniel Kartika Sari Dewi Kurniati, Neng F Manguntungi, Baso Mustafawi, Wike Zahra Neng Fisheri Kurniati Novianti, Ela Nur Amani, Febriyanti Nur Umami, Rifqiyah RATIH ASMANA NINGRUM Rifqiyah Nur Umami, Rifqiyah Nur Saputri, Dinar Suksmayu Saputri Sari, Isti Kartika Sri Swasthikawati, Sri Vanggy, Leggina Rezzy Wijaya, Hans Yudi Purnomo