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All Journal Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Makara Journal of Science
Kusnanda, Prima Sekti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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The Potency of Organic Fertilizer in Reducing Leaf Curl Disease on Chili (Capsicum annuum L.): Potensi Pupuk Organik Dalam Mengurangi Penyakit Keriting Daun Pada Cabai (Capsicum annuum L.) Subiastuti, Aprilia Sufi; Windianingsih, Diah Fitri; Noviyani, Pipit; Kusnanda, Prima Sekti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 1 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v12i1.418

Abstract

Chili is an essential commodity in Indonesia, with demand continuing to rise; however, its production is often disrupted by leaf curl disease caused by viruses. One potential solution is using organic fertilizer enriched with beneficial microbes such as Plant Growth-Promoting Microorganisms (PGPM). Despite its potential, the role of PGPM in controlling viral diseases in chili plants has been rarely studied. This study aims to explore the effectiveness of organic fertilizer in reducing leaf curl infections by improving plant health. Two chili cultivars were used: 'Gama LBF' (a breeding product of PIAT UGM) and 'Ponirun' (a commercial variety known for its disease resistance). The chili plants were treated with various concentrations of organic fertilizer, and their effectiveness was evaluated based on disease incidence (the number of symptomatic plants) and disease severity (the level of visual symptoms), which were then converted into an index. Results showed that the 75% concentration yielded the lowest disease incidence in both cultivars—26.5% for 'Ponirun' and 42.85% for 'Gama LBF'. The lowest disease severity was also observed at the 75% concentration for 'Ponirun' and 50% for 'Gama LBF'. These findings highlight the importance of selecting the appropriate organic fertilizer concentration to suppress leaf curl disease in chili effectively.
Inter-Simple Sequence Repeats Application on the Identification of Genetic Variation of a New Indonesian Melon Cultivar “Melona” Salsabila, Tantri Ajeng Salma; Yusuf, Adib Fakhruddin; Wibowo, Wiko Arif; Kusnanda, Prima Sekti; Daryono, Budi Setiadi
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

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Abstract

The field of identification of molecular markers has seen significant advancements, with the Inter-Simple Sequence Repeat (ISSR) emerging as a prominent tool for characterizing genetic variation within species. This study focuses on the new Indonesian melon cultivar “Melona,” developed through selective breeding of commercial melons. This study aimed to assess the utility of ISSR markers in evaluating the genetic variation and stability of the “Melona” cultivar. Comparisons were made with established cultivars—‘Inthanon,” “Rich Sweetness,” “Chamoe,” and “Hikapel”—using five ISSR primers: UBC 807, UBC 808, UBC 810, UBC 812, and UBC 826. A total of 70 DNA fragments with a high polymorphism rate were produced, thereby providing insight into phenetic relationships among the cultivars with simi-larity indices <70%. These genetic distinctions were corroborated through morphological evaluations. These findings indicate that ISSR markers effectively reveal genetic variation within species, offering a practical approach for devel-oping new plant varieties in the Cucurbitaceae family.
Co-Authors BUDI SETIADI DARYONO Noviyani, Pipit Salsabila, Tantri Ajeng Salma Subiastuti, Aprilia Sufi Wibowo, Wiko Arif Windianingsih, Diah Fitri Yusuf, Adib Fakhruddin