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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Riset Biologi dan Aplikasinya
Wibowo, Wiko Arif
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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Downy Mildew Infection in Indonesian Melon Cultivar 'Melona' Based on Morphological and Anatomical Characters Angellya, Bunga Finovel; Yusuf, Adib Fakhruddin; Wibowo, Wiko Arif; Daryono, Budi Setiadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8597

Abstract

Melons are a horticultural crop of the Cucurbitaceae family with high economic value and worldwide distribution. The 'Melona' variety is the result of breeding selection from commercial melons in Indonesia and has a golden yellow skin color with lobes, crisp flesh, and a high degree of sweetness. Downy mildew is a fungal infection that attacks the leaves of the plant causing brownish-yellow-colored symptoms. Downy mildew can cause crop failure under extreme conditions as the plant loses its productive capacity. Infection levels were observed by scoring and calculating disease index scores. The morphology of healthy and diseased plants was documented and analyzed descriptively. Anatomical features of healthy and diseased leaves were compared using anatomical observations on leaf cross-sections. Quantitative data analysis was carried out using the T-test analysis method with a 5% significance level. Melona's resistance to downy mildew is in the tolerant to susceptible category. Chlorosis and necrotic spots on the leaves and stunted growth are symptoms of downy mildew infection. Healthy leaves had a greater axial and abaxial epidermal thickness, a lower mesophyll thickness, a smaller cell size and a better quantity and quality of trichomes than leaves infected with downy mildew.
Assessing Foliar Chlorophyll Content with SPAD-502 Chlorophyll Meter: A Comparison with Spectrophotometer Method in Various Plants Nurbaiti, Siti; Milasari, Asri Fajar; Wibowo, Wiko Arif; Nilamsari, Elvian Indah; Rachmawati, Diah
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p50-56

Abstract

Measuring chlorophyll content in plants is one of the main points that has never been ignored in various plant biology and agronomy research studies. Chlorophyll content is a parameter of growth and development, diagnosis of nutritional status, and response to environmental conditions. This study aimed to compare and determine the relationship between SPAD-502 chlorophyll meter readings and chlorophyll content obtained from acetone extraction followed by spectrophotometry measurement. Various leaves with different colors and thicknesses were used to determine the reliability of SPAD readings across diverse morphological traits. The results showed higher SPAD readings in leaves with a greener color. SPAD value was found to have a good linear relationship (r 0.8 and R2 0.64) and a positive correlation with total chlorophyll content, though variations due to leaf thickness suggest the need for correction factors. Furthermore, SPAD has potential as a rapid, non-destructive tool for monitoring plant health and nutrient status in agriculture, plant breeding, and horticulture. This tool can contribute to optimizing crop yield and managing fertilization practice, especially where the maintaining leaf integrity is essential for both commercial and aesthetic value. Species-specific calibration models are recommended to enhance measurement accuracy.
Co-Authors Angellya, Bunga Finovel BUDI SETIADI DARYONO DIAH RACHMAWATI Milasari, Asri Fajar Nilamsari, Elvian Indah Siti Nurbaiti Yusuf, Adib Fakhruddin