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Downy Mildew Infection in Indonesian Melon Cultivar 'Melona' Based on Morphological and Anatomical Characters Angellya, Bunga Finovel; Yusuf, Adib Fakhruddin; Wibowo, Wiko Arif; Daryono, Budi Setiadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8597

Melons are a horticultural crop of the Cucurbitaceae family with high economic value and worldwide distribution. The 'Melona' variety is the result of breeding selection from commercial melons in Indonesia and has a golden yellow skin color with lobes, crisp flesh, and a high degree of sweetness. Downy mildew is a fungal infection that attacks the leaves of the plant causing brownish-yellow-colored symptoms. Downy mildew can cause crop failure under extreme conditions as the plant loses its productive capacity. Infection levels were observed by scoring and calculating disease index scores. The morphology of healthy and diseased plants was documented and analyzed descriptively. Anatomical features of healthy and diseased leaves were compared using anatomical observations on leaf cross-sections. Quantitative data analysis was carried out using the T-test analysis method with a 5% significance level. Melona's resistance to downy mildew is in the tolerant to susceptible category. Chlorosis and necrotic spots on the leaves and stunted growth are symptoms of downy mildew infection. Healthy leaves had a greater axial and abaxial epidermal thickness, a lower mesophyll thickness, a smaller cell size and a better quantity and quality of trichomes than leaves infected with downy mildew.

Assessing Foliar Chlorophyll Content with SPAD-502 Chlorophyll Meter: A Comparison with Spectrophotometer Method in Various Plants Nurbaiti, Siti; Milasari, Asri Fajar; Wibowo, Wiko Arif; Nilamsari, Elvian Indah; Rachmawati, Diah
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p50-56

Measuring chlorophyll content in plants is one of the main points that has never been ignored in various plant biology and agronomy research studies. Chlorophyll content is a parameter of growth and development, diagnosis of nutritional status, and response to environmental conditions. This study aimed to compare and determine the relationship between SPAD-502 chlorophyll meter readings and chlorophyll content obtained from acetone extraction followed by spectrophotometry measurement. Various leaves with different colors and thicknesses were used to determine the reliability of SPAD readings across diverse morphological traits. The results showed higher SPAD readings in leaves with a greener color. SPAD value was found to have a good linear relationship (r 0.8 and R2 0.64) and a positive correlation with total chlorophyll content, though variations due to leaf thickness suggest the need for correction factors. Furthermore, SPAD has potential as a rapid, non-destructive tool for monitoring plant health and nutrient status in agriculture, plant breeding, and horticulture. This tool can contribute to optimizing crop yield and managing fertilization practice, especially where the maintaining leaf integrity is essential for both commercial and aesthetic value. Species-specific calibration models are recommended to enhance measurement accuracy.

Pembuatan sediaan serum dari ekstrak Gama Melon Parfum (GMP) di Laboratorium Farmasi Universitas Gadjah Mada Masita, Masita; Masriany, Masriany; Sulaiman, â€ªTeuku Nanda Saifulllah; Wibowo, Wiko Arif; Daryono, Budi Setiadi
Filogeni: Jurnal Mahasiswa Biologi Vol 4 No 1 (2024): Januari-April
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v4i1.34997

Kosmetik menurut Food and Drug Administration merupakan produk yang digunakan pada kulit dengan tujuan untuk membersihkan, mempercantik, memperbaiki diri ataupun untuk meningkatkan daya tarik. Berdasarkan Peraturan Kepala BPOM nomor 18 tahun 2015 tentang persyaratan teknis bahan kosmetik, bahan kimia pada kosmetik dibedakan menjadi dua, yaitu bahan kimia berbahaya yang dilarang dan bahan kimia berbahaya yang diperbolehkan digunakan dengan pembatasan dalam penggunaannya pada produk kosmetik. Namun pada dasarnya, kosmetik tidak hanya terbuat dari bahan kimia melainkan dapat pula terbuat dari bahan alami seperti ekstrak buah yang dimanfaatkan. Salah satu tumbuhan yang dapat dijadikan bahan alami dalam pembuatan kosmetik yaitu buah melon. Jenis penelitian merupakan penelitian kualitatif yang terfokus pada proses pembuatan sediaan serum dari ekstrak melon kultivar Gama Melon Parfum yang diperoleh melalui metode dekokta (pemanasan). Hasil yang diperoleh yaitu diketahui adanya batasan-batasan dalam pembuatan kosmetik terkhusus pada penambahan bahan dan perlakuan yang membantu keberhasilan pembuatan serum, seperti pengadukan campuran harus dilakukan secara konstan, jika tidak maka akan menimbulkan buih atau gelembung yang menyebabkan pH serum tidak stabil.

Inter-Simple Sequence Repeats Application on the Identification of Genetic Variation of a New Indonesian Melon Cultivar “Melona” Salsabila, Tantri Ajeng Salma; Yusuf, Adib Fakhruddin; Wibowo, Wiko Arif; Kusnanda, Prima Sekti; Daryono, Budi Setiadi
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

The field of identification of molecular markers has seen significant advancements, with the Inter-Simple Sequence Repeat (ISSR) emerging as a prominent tool for characterizing genetic variation within species. This study focuses on the new Indonesian melon cultivar “Melona,” developed through selective breeding of commercial melons. This study aimed to assess the utility of ISSR markers in evaluating the genetic variation and stability of the “Melona” cultivar. Comparisons were made with established cultivars—‘Inthanon,” “Rich Sweetness,” “Chamoe,” and “Hikapel”—using five ISSR primers: UBC 807, UBC 808, UBC 810, UBC 812, and UBC 826. A total of 70 DNA fragments with a high polymorphism rate were produced, thereby providing insight into phenetic relationships among the cultivars with simi-larity indices <70%. These genetic distinctions were corroborated through morphological evaluations. These findings indicate that ISSR markers effectively reveal genetic variation within species, offering a practical approach for devel-oping new plant varieties in the Cucurbitaceae family.

Downy Mildew Infection in Indonesian Melon Cultivar 'Melona' Based on Morphological and Anatomical Characters Angellya, Bunga Finovel; Yusuf, Adib Fakhruddin; Wibowo, Wiko Arif; Daryono, Budi Setiadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8597

Melons are a horticultural crop of the Cucurbitaceae family with high economic value and worldwide distribution. The 'Melona' variety is the result of breeding selection from commercial melons in Indonesia and has a golden yellow skin color with lobes, crisp flesh, and a high degree of sweetness. Downy mildew is a fungal infection that attacks the leaves of the plant causing brownish-yellow-colored symptoms. Downy mildew can cause crop failure under extreme conditions as the plant loses its productive capacity. Infection levels were observed by scoring and calculating disease index scores. The morphology of healthy and diseased plants was documented and analyzed descriptively. Anatomical features of healthy and diseased leaves were compared using anatomical observations on leaf cross-sections. Quantitative data analysis was carried out using the T-test analysis method with a 5% significance level. Melona's resistance to downy mildew is in the tolerant to susceptible category. Chlorosis and necrotic spots on the leaves and stunted growth are symptoms of downy mildew infection. Healthy leaves had a greater axial and abaxial epidermal thickness, a lower mesophyll thickness, a smaller cell size and a better quantity and quality of trichomes than leaves infected with downy mildew.

Antioxidant Activity in Melon (Cucumis melo L. 'Gama Melon Parfum') as Antiaging Cream Formulation Wahyuni, Satria; Wibowo, Wiko Arif; Sulaiman, Teuku Nanda Saifullah; Daryono, Budi Setiadi
Biotropika: Journal of Tropical Biology Vol. 11 No. 3 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.03.05

Antiaging cosmetics are becoming increasingly popular among the general public. Melon (Cucumis melo L.) Gama Melon Parfum cultivars ('GMP') are one of the plants that can be utilized as a basic ingredient in the production of antiaging cosmetics. 'GMP' melon has cucurbitacin compounds that can be utilized as antiaging and contains flavonoid, saponin, and terpenoid compounds that have antioxidant properties. This research aims to analyze the difference in antioxidant activity of 'GMP' melon extract and 'GMP' melon cosmetic cream preparation cultivated in Yogyakarta with 'GMP' melon cultivated in Bandung, analyze extract standardization parameters, and determine the phenolic and flavonoid contents of each melon extract contains. The activity of antioxidants was determined by the DPPH method, the Folin-Ciocelteu method to measure phenolics, and the AlCl3 solution-based colorimetric approach to measuring flavonoid content. It was revealed that the highest % RSA of antioxidant activity is in ‘GMP’ melon extract cultivated in Bandung. The results of the standardization of 'GMP' melon extract met the requirements of standard parameters. The greatest total phenolic content is derived from the extract of ‘GMP’ melon in Bandung, while the highest total flavonoid content in 'GMP' melon extracts is cultivated in Yogyakarta. The highest to lowest antioxidant activity of ‘GMP’ melon cream was formula IV (‘GMP’ melon extract 30% cultivated in Bandung), formula III (‘GMP’ melon extract 20% grown in Bandung), formula II (‘GMP’ melon extract 30% cultivated in Yogyakarta), formula I (‘GMP’ melon extract 20% cultivated in Yogyakarta), and the lowest is in K- with formula without ‘GMP’ melon extract.

Physiological responses and production of Gama Melon Parfum (Cucumis melo L. cv. GMP) on different water availability Putri Renata, Nellis Nadinda; Daryono, Budi Setiadi; Wibowo, Wiko Arif; Rachmawati, Diah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 3 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i3.54022

Gama Melon Parfum (GMP) cultivar is a result of crossbreeding between the Natsuno Omoide and Miyamauri melon varieties in 2011. GMP exhibits a unique phenotypic characteristic, including a bitter taste of the fruit flesh and produces a stronger aroma. The objective of this study was to investigate the physiological responses and productivity of GMP under varying water conditions. The experiment was conducted using a single factor consisting of different levels of water availability with field capacity at 50%, 75%, and 100%, as well as submergence at 2 cm, 4 cm, and 8 cm above the soil surface. Each treatment was replicated three times. The plot size for each replication was 2 m x 2 m, ensuring consistent conditions for all treatments. The results of the study showed that treatment with 100% field capacity increased stem diameter, while treatment with 50% field capacity increased the root-to-shoot ratio of GMP. Submergence treatment at 8 cm decreased stem diameter and the root-to-shoot ratio of GMP. The 50% field capacity treatment reduced the total chlorophyll levels in GMP leaves. Submergence treatment at 8 cm increased the total chlorophyll levels in GMP leaves. The 50% field capacity treatment increased fruit fresh weight, while submergence treatment at 2 cm decreased fruit fresh weight. Submergence treatment at 2 cm reduced fruit water content, whereas submergence treatment at 8 cm increased water content in GMP fruits. Keywords: drought; Gama Melon Parfume (GMP); growth; plant physiological responses; submergence

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