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All Journal JURNAL ANALIS LABORATORIUM MEDIK Bioscientist : Jurnal Ilmiah Biologi
Setyoningrum, Fransisca Probo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Social Sciences

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PERBANDINGAN METODE DEHIDRASI TERHADAP KUALITAS PREPARAT PERMANEN LARVA NYAMUK Culex sp. Onesiforus, Benaya Yamin; Setyoningrum, Fransisca Probo; San, Maria Pritinsia Cici
Jurnal Analis Laboratorium Medik Vol 10 No 1 (2025): Jurnal Analis Laboratorium Medik
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v10i1.5527

Abstract

Proses pembuatan preparat larva nyamuk memiliki peran penting dalam dunia kesehatan untuk proses identifikasi. Salah satu tahap penitng dalam pembuatan preparat permanen larva nyamuk adalah tahap dehidrasi, yang dilakukan melalui perendaman larva kedalam alkohol. Banyaknya variasi pada prosedur perendaman etanol menjadikan belum adanya standar baku untuk proses dehidrasi dalam pembuatan preparat permanen larva nyamuk. Tujuan dari penelitian ini untuk mengetahui perbandingan 3 metode dehidrasi alkohol terhadap kualitas preparat permanen larva nyamuk. Penelitian ini merupakan penelitian eksperimental dengan menggunakan variasi dehidrasi etanol bertingkat. Jenis data yang diperoleh berupa data kategorik ordinal dan uji statistika yang digunakan dalam penelitian ini menggunakan uji Mann-Whitney U, pada kejernihan preparat didapatkan hasil, perlakuan alkohol 70% vs 30%,50%,96% nilai p = 0,609, alkohol 70% vs 60%,80%,90% nilai p = 0,159, antar alkohol bertingkat = 30%,50%,96% alkohol bertingkat 60%,80%,90% didapatkan nilai p = 0,357. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan, tidak terdapat perbedaan yang signifikan antara metode perendaman alkohol terhadap kualitas preparat permanen lava nyamuk
Effect of Variations in Blood Component Supplementation on the Suitability of Blood Agar Plate Media for the Growth of Salmonella typhi Setyoningrum, Fransisca Probo
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19748

Abstract

This study aimed to evaluate the effect of different human blood components added to Blood Agar Plate (BAP) media on the growth of Salmonella typhi O. An experimental design was employed with three treatment groups and a total of 27 replicates. Salmonella typhi O was inoculated onto BAP media containing different blood components, followed by incubation at 37°C for 24 hours. Bacterial colonies were then counted using the total plate count method. Statistical analysis was performed using the Shapiro–Wilk test and one-way ANOVA, followed by Bonferroni post hoc analysis. The results showed that variation in blood components significantly affected colony counts (p = 0.0001). Whole blood containing antibodies produced the lowest bacterial growth (mean 2.6 × 10⁵ CFU/mL), whereas erythrocyte suspension (1.8 × 10⁶ CFU/mL) and whole blood without antibodies (1.9 × 10⁶ CFU/mL) showed substantially higher growth and did not differ significantly from each other (p = 0.618). These findings indicate that antibodies in human blood can inhibit the growth of Salmonella typhi through complement activation, while washed erythrocytes are effective as a substitute for sheep blood. Therefore, whole blood without antibodies or erythrocyte suspension may serve as viable and effective alternatives for the preparation of BAP media in laboratories with limited access to sheep blood.
Co-Authors Benaya Yamin Onesiforus San, Maria Pritinsia Cici