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All Journal International Journal of Cell and Biomedical Science Calvaria Medical Journal Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran
Rachmawati, Nurlaely Mida
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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STUDI HISTOPATOLOGI PENGARUH PEMBERIAN DMBA SECARA TOPIKAL PADA ORGAN HATI MENCIT C3H/HEJ Ariany, Devy; Rahman, Abd; Rachmawati, Nurlaely Mida; Hermawati, Luluk; Irawati, Nur Bebi Ulfah
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran Vol. 10 No. 1 (2025): Maret
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/mtj.v10i1.1892

Abstract

Optimalisasi Waktu Fiksasi Menggunakan Formalin 10% Pada Ginjal, Hepar, dan Pankreas Tikus Sprague Dawley Untuk Pewarnaan Haematoxylin-Eosin Alwi, Muhammad Azharan; Rachmawati, Nurlaely Mida; Ariany, Devy; Hermawati, Luluk; Zulfa, Hilizza Awalina
Calvaria Medical Journal Vol 3 No 2 (2025): Edisi Desember 2025
Publisher : Institute for Research and Community Services (LPPM) of University of Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/kp751w77

Abstract

Background: Histotechnology is a series of procedures used to prepare histological specimens for microscopic analysis. One of the critical steps in this process is fixation, which serves to preserve tissue structure and prevent changes in shape or size. However, excessive fixation duration may lead to over-hardening of tissues, resulting in reduced quality of histological observations. Objectives: To evaluate the feasibility of two week formalin 10% fixation period on histological quality of kidney, liver, and pancreas tissues. Methods: After a 14-day adaptation period, male Sprague Dawley rats were anesthetized, necropsied, and their kidney, liver, and pancreas were fixed in 10% formalin. Histotechnical procedures included graded alcohol dehydration, toluene clearing, paraffin embedding, 6 µm sectioning, and HE staining. Preparations were observed using an Olympus BX41 microscope at magnifications of 4x–40x. Results: Two-week fixation caused structural damage to the kidney, liver, and pancreas. Damage observed included glomeruli and tubules in the kidney, the portal triad structure in the liver, and the Langerhans islet cells in the pancreas. Statistical results (if any) should be included here. Conclusions: Fixation for two weeks did not produce adequate histological preparations, and therefore, it is not recommended as a reference for developing histotechnology SOPs in related laboratories.
Preliminary Study on Long Fixation in Histological Preparations of Internal Organs of Sprague Dawley Rats Prahanarendra, Galang; Ariany, Devy; Rachmawati, Nurlaely Mida; Hermawati , Luluk; Putri, Ghea Farmaning Thias
International Journal of Cell and Biomedical Science Vol 4 No 12 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i12.77

Abstract

Histotechnology is a series of processes ranging from tissue handling to the preparation of slides that can be observed under a microscope. One crucial stage in this process is fixation, which serves to preserve the structure and morphology of the tissue as close as possible to its original physiological state. However, prolonged fixation duration may lead to tissue hardening, dissolution, and structural damage. This study aims to obtain supporting data for the development of a standard operating procedure (SOP) in histotechnology that can be applied in the animal house and histology laboratories of the Faculty of Medicine, Syarif Hidayatullah State Islamic University Jakarta. The results showed that fixation for three weeks caused morphological damage to the kidney, liver, and pancreas of Sprague Dawley rats. The findings included tissue perforation in all three organs, endothelial nuclear damage in the kidney, central vein wall damage in the liver, and cellular disintegration in the pancreatic islets of Langerhans. Based on these findings, it can be concluded that a fixation duration of three weeks does not produce optimal histological images and therefore cannot be used as a reference for establishing a standard histotechnology SOP in the laboratory of the Faculty of Medicine, Syarif Hidayatullah State Islamic University Jakarta.
Co-Authors Abd Rahman Alwi, Muhammad Azharan Ariany, Devy Ghea Farmaning Thias Putri Irawati, Nur Bebi Ulfah Luluk Hermawati Prahanarendra, Galang Zulfa, Hilizza Awalina