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All Journal Journal of Islamic Medicine Calvaria Medical Journal Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran
Ariany, Devy
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Efektivitas Ekstrak Daun Kelor (Moringa oleifera) Terhadap Kadar Trigliserida dan Glukosa Darah Sewaktu pada Tikus Jantan Galur Sprague Dawley Diabetes Melitus Muniroh, Muniroh; Masnunah, Masnunah; Ariany, Devy
Journal of Islamic Medicine Vol 8, No 2 (2024): JOURNAL OF ISLAMIC MEDICINE EDISI SEPTEMBER 2024
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jim.v8i2.29672

Abstract

Background: According to data from the International Diabetes Federation (IDF), Indonesia ranks 5th in the world for the highest number of diabetes mellitus cases, with numbers predicted to continue rising. Dyslipidemia is one of the complications of diabetes mellitus, potentially leading to coronary heart disease. Kelor leaves (Moringa oleifera) contain flavonoids, which may reduce blood glucose and triglyceride levels. Objective: This study aims to evaluate the effect of Moringa oleifera leaf extract on blood glucose and triglyceride levels in male Sprague dawley rats. Methods: This experimental study involved 24 male Sprague dawley rats. Blood glucose and triglyceride levels were measured before and after administering Moringa oleifera leaf extract. The Shapiro-Wilk test assessed data normality, followed by paired t-tests, One-way ANOVA, and Pearson correlation. Results: Moringa oleifera leaf extract at a 400 mg/kg BW dose significantly reduced blood glucose levels (p=0.017). No statistically triglyceride levels decreased in all groups (p=0.195). Conclusion: Moringa oleifera leaf extract can significantly lower blood glucose levels at a dose of 400 mg/kg BW. Further research is needed to investigate the effects of Moringa oleifera on triglyceride levels
STUDI HISTOPATOLOGI PENGARUH PEMBERIAN DMBA SECARA TOPIKAL PADA ORGAN HATI MENCIT C3H/HEJ Ariany, Devy; Rahman, Abd; Rachmawati, Nurlaely Mida; Hermawati, Luluk; Irawati, Nur Bebi Ulfah
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran Vol. 10 No. 1 (2025): Maret
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/mtj.v10i1.1892

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Optimalisasi Waktu Fiksasi Menggunakan Formalin 10% Pada Ginjal, Hepar, dan Pankreas Tikus Sprague Dawley Untuk Pewarnaan Haematoxylin-Eosin Alwi, Muhammad Azharan; Rachmawati, Nurlaely Mida; Ariany, Devy; Hermawati, Luluk; Zulfa, Hilizza Awalina
Calvaria Medical Journal Vol 3 No 2 (2025): Edisi Desember 2025
Publisher : Institute for Research and Community Services (LPPM) of University of Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/kp751w77

Abstract

Background: Histotechnology is a series of procedures used to prepare histological specimens for microscopic analysis. One of the critical steps in this process is fixation, which serves to preserve tissue structure and prevent changes in shape or size. However, excessive fixation duration may lead to over-hardening of tissues, resulting in reduced quality of histological observations. Objectives: To evaluate the feasibility of two week formalin 10% fixation period on histological quality of kidney, liver, and pancreas tissues. Methods: After a 14-day adaptation period, male Sprague Dawley rats were anesthetized, necropsied, and their kidney, liver, and pancreas were fixed in 10% formalin. Histotechnical procedures included graded alcohol dehydration, toluene clearing, paraffin embedding, 6 µm sectioning, and HE staining. Preparations were observed using an Olympus BX41 microscope at magnifications of 4x–40x. Results: Two-week fixation caused structural damage to the kidney, liver, and pancreas. Damage observed included glomeruli and tubules in the kidney, the portal triad structure in the liver, and the Langerhans islet cells in the pancreas. Statistical results (if any) should be included here. Conclusions: Fixation for two weeks did not produce adequate histological preparations, and therefore, it is not recommended as a reference for developing histotechnology SOPs in related laboratories.
Co-Authors Abd Rahman Alwi, Muhammad Azharan Irawati, Nur Bebi Ulfah Luluk Hermawati Masnuna, Masnuna Muniroh, Muniroh Rachmawati, Nurlaely Mida Zulfa, Hilizza Awalina