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All Journal Proceeding of International Conference Health, Science And Technology (ICOHETECH) Prosiding Seminar Informasi Kesehatan Nasional
Aminnulloh, Muhammad
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health

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UJI PERBANDINGAN TOTAL FLAVONOID EKSTRAK ETANOL 70% DAN ETANOL 96% DAUN KIRINYUH (CHROMOLAENA ODORATA L.) Aminnulloh, Muhammad; Fitriawati, Anna; Wardani, Tatiana Siska
Prosiding Seminar Informasi Kesehatan Nasional 2025: SIKesNas 2025
Publisher : Fakultas Ilmu Kesehatan Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47701/afn03x65

Abstract

Tanaman kirinyuh (Chromolaena odorata L.) sering dianggap sebagai tanaman pengganggu (gulma) di kalangan masyarakat umum. Penelitian dilakukan secara in vitro untuk mengetahui perbandingan kadar total flavonoid yang lebih tinggi dari pelarut etanol 70% dan 96% ekstrak daun kirinyuh. Penetapan kadar total flavonoid ekstrak etanol 70% dan 96% daun kirinyuh dilakukan dengan metode kalorimetri AlCl3 dengan kuersetin sebagai larutan standar Kemudian menyiapkan lima tabung reaksi dengan konsentrasi kuersetin masing-masing 40, 60, 80, 100, dan 120 ppm. Selanjutnya, ekstrak tersebut dicampurkan dengan larutan reagen AlCl3 10% dalam tabung reaksi baru dengan rasio 1:1, yaitu 1 mL ekstrak dicampur dengan 1 mL larutan reagen AlCl3 dan ditambah 8 mL larutan CH3COOH 5%. Hasil campuran ini kemudian dianalisis dengan menggunakan spektrofotometer Genesys 30-UV Vis pada panjang gelombang 425 nm untuk membuat kurva standar. Hasil diperoleh dari pengujian dengan metode kalorimetri yaitu ekstrak etanol 70% dan 96% daun kirinyuh dengan kadar total flavonoid 51,415 ± 0,462 mg QE/g dan 61,108 ± 3,030 mg QE/g. Dapat dilihat bahwa ekstrak etanol 96% memiliki kadar total flavonoid yang lebih tinggi dibandingkan dengan ekstrak etanol 70%, hal tersebut dikarenakan etanol 96% memiliki daya tarik senyawa yang lebih kuat untuk menarik senyawa flavonoid dari ekstrak daun kirinyuh daripada etanol 70%.
ANTIHYPERURESEMIA ACTIVITY OF ETHANOL EXTRACTS AND FRACTIONS OF NIPAH LEAF FRONDS (NYPA FRUTICANS. WURMB) Raharjo, Danang; Dwi S, Anita; Aminnulloh, Muhammad
Proceeding of the International Conference Health, Science And Technology (ICOHETECH) 2023: Proceeding of the 4th International Conference Health, Science And Technology (ICOHETECH)
Publisher : LPPM Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47701/icohetech.v4i1.3397

Abstract

Hyperuresemia is a metabolite syndrome characterised by high levels of uric acid in the blood. Hyperuresmia in advanced stages can cause gout or pyrexia (a disease that affects the joints and causes joint damage), kidney stones, kidney damage, and hypertension. The nipah plant (Nypa fruticans. Wurmb) is traditionally used in the treatment of stomach pain, diabetes, fever, toothache, uric acid reduction and headache medicine. In general, the content of secondary metabolite compounds from nipah plants includes flavonoids, phenols, tannins, terpenois, steroids, alkaloids and saponins. The purpose of this study was to determine the xanthine oxidase inhibitory activity of ethanol extracts and fractions of nipah leaves (Nypa fruticans. Wurmb). Extraction process using maceration method with 96% ethanol solvent, fractionation using liquid-liquid partition method with separating funnel. . Testing the inhibition of xanthine oxidase enzyme activity in vitro using UV/VIS spectrophotometer. The results showed that ethanol extract, water fraction, ethyl acetate fraction and n-hexane fraction showed inhibitory activity of xanthine oxidase enzyme with IC50 values of 47.558 ± 2.862; 18.755 ± 3.821; 3.543 ± 1.440 and 110.213 ± 1.577 ppm, respectively. From the results of the study it can be concluded that ethanol extracts and fractions of nipah leaf fronds (Nypa fruticans.Wurmb) can inhibit the activity of xanthine oxidase enzyme with ethyl acetate fraction giving the highest inhibitory activity with IC50 value of 3,543±1,440 ppm.
Co-Authors Anna Fitriawati Danang Raharjo Dwi S, Anita Wardani, Tatiana Siska