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All Journal Jurnal Riset Rumpun Ilmu Tanaman Al-Hayat: Natural Sciences, Health & Environment Journal Jurnal Pendidikan Kimia, Fisika dan Biologi Inventa: Journal of Science, Technology, and Innovation
Devi Bunga Pagalla
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Agriculture, Biological Sciences & Forestry Automotive Engineering Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Public Health

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Sterilisasi Optimal untuk Kultur Jaringan Bambu Kuning (Bambusa Vulgaris) Secara in Vitro Miftahul Fikriyah Adudu; Novri Youla Kandowangko; Devi Bunga Pagalla; Jusna Ahmad; Indriati Husain; Lindawaty Isima
Al-Hayat: Natural Sciences, Health & Environment Journal Vol. 3 No. 1 (2025): Al-Hayat: Natural Sciences, Health & Environment Journal 
Publisher : Institut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47467/alhayat.v3i1.6255

Abstract

Yellow bamboo has great potential in various fields, but its conventional propagation has several obstacles. Tissue culture offers a solution to overcome these problems. The focus of this research is the use of appropriate sterilization for in vitro culture. Effective sterilization is a crucial first step in the success of tissue culture. In this study using sterilization in the form of using Nordox 56 WP, without using Nordox 56WP, using Antracol 5 gr solution, and using HgCl2. The parameters observed were the appearance of contaminants in the form of fungi and bacteria and the time of death of the explants. The results showed that the use of HgCl2 can inhibit contaminants and the time of explant death in yellow bamboo plants in vitro.
Analisis Proksimat Jagung Pulut (Zea mays L. var Damahu) dari Desa Bontula Kecamatan Asparaga Kabupaten Gorontalo Mustamin Ibrahim; Devi Bunga Pagalla; Abubakar Sidik Katili; Nurul Fajryani Usman
Jurnal Riset Rumpun Ilmu Tanaman Vol. 4 No. 2 (2025): Oktober : JURRIT: Jurnal Riset Rumpun Ilmu Tanaman
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrit.v4i2.7254

Abstract

The Damahu waxy corn (Zea mays L. var. Damahu) is a local variety traditionally cultivated by the Gorontalo community and possesses significant potential for the development of functional food products. This study aimed to determine the nutritional composition of the Damahu waxy corn variety originating from Bontula Village, Asparaga District, Gorontalo Regency, through proximate analysis. The corn samples were dried, milled, and analyzed using standard analytical procedures to quantify moisture, ash, fat, protein, and carbohydrate contents. The results indicated that Damahu waxy corn flour contained 83.32% carbohydrates, 5.93% protein, 1.67% fat, 8.84% moisture, and 0.24% ash. This composition characterizes the Damahu variety as a good energy source. However, its protein content does not yet meet the minimum requirement set by the Indonesian National Standard (SNI) for corn-based processed products. The nutritional profile highlights the potential of Damahu waxy corn for the development of nutritious and competitive local food products. This study provides an initial contribution to the documentation of Gorontalo’s indigenous corn varieties and offers a scientific foundation for the utilization of local germplasm to strengthen regional food security.
Pengaruh Ekstrak Tauge terhadap Perkecambahan Benih Padi (Oryza Sativa L.) yang Lewat Masa Simpan Mega Rosalita Ekaputri Koni; Jusna Ahmad; Devi Bunga Pagalla
Jurnal Pendidikan Kimia, Fisika dan Biologi Vol. 2 No. 1 (2026): Januari : Jurnal Pendidikan Kimia, Fisika dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jupenkifb.v2i1.926

Abstract

The decline in seed quality due to storage beyond the shelf life is a major problem in rice cultivation. One effort that can be made to improve seed viability is through invigoration techniques using natural ingredients, such as bean sprout extract (Phaseolus radiatus), which contains growth hormones. This study aims to analyze the effect of bean sprout extract on the viability of Ciherang rice seeds that have exceeded their shelf life and to determine the best treatment. The study was conducted from August to November 2024 at the Biology Laboratory of the UPTD Seed Center, Supervision and Certification of Agricultural Seeds of Gorontalo Province. The study used a two-factor Randomized Block Design (RBD) with bean sprout age (3, 5, and 7 days after sowing) and bean sprout extract concentration (20 g/L, 40 g/L, and 60 g/L) as factors, with four replications. The parameters observed included germination rate, maximum growth potential, and sprout length. The data were analyzed using ANOVA and DMRT post-hoc test at a 5% level. The results showed that bean sprout extract had a significant effect on all observed parameters. The treatment of 5-day-old bean sprouts with a concentration of 20 g/L gave the best results with a germination rate of 95.5%, maximum growth potential of 98.5%, and the highest sprout length. Sprout extract has the potential to be used as a natural alternative to improve the quality of rice seeds that have passed their storage period.
Induksi Kalus dari Antera Pisang Goroho (Musa acuminata L.) dengan Menggunakan 2,4-D dan BAP Risna A. Onu; Jusna Ahmad; Febriyanti, Febriyanti; Novri Youla Kandowangko; Devi Bunga Pagalla; Indriati Husain
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/8t6ph238

Abstract

Callus induction is a critical stage in anther culture for supporting the propagation and genetic improvement of local banana cultivars. This study evaluated the effect of different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction from goroho banana (Musa acuminata L.) anthers. Observations were conducted for 30 days after induction, focusing on callus emergence time and morphological characteristics, including color and texture. The results demonstrated distinct responses among treatments. The combination of 1.5 ml 2,4-D and 1.5 ml BAP produced the fastest callus initiation with an average of 13.17 days after induction and generated friable callus with a pale yellow coloration, indicating high cellular activity. Lower growth regulator concentrations delayed callus formation, while higher concentrations promoted color changes associated with early tissue differentiation. No callus formation was observed in the absence of exogenous growth regulators. These findings highlight the importance of balanced auxin and cytokinin concentrations in regulating anther-derived callus induction and provide a physiological basis for further development of goroho banana anther culture systems.
Co-Authors Abubakar Sidik Katili febriyanti febriyanti Indriati Husain Jusna Ahmad Lindawaty Isima Mega Rosalita Ekaputri Koni Miftahul Fikriyah Adudu Mustamin Ibrahim Novri Youla Kandowangko Nurul Fajryani Usman Risna A. Onu