Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Journal of Agromedicine and Medical Sciences Biology, Medicine, & Natural Product Chemistry
Andriani, Made Prasanti
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Computational Insights into Leucaena leucocephala Extract Shampoo as a Malassezia furfur Anti-Dandruff Agent Hibatulloh, Muhammad Farhan; Nafisa, Tsabita; Afnan, Muhammad Syifaul; Andriani, Made Prasanti; Widanto, Cika Abadi Laily; Wisudanti, Desie Dwi
Journal of Agromedicine and Medical Sciences Vol. 11 No. 2 (2025)
Publisher : Faculty of Medicine, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v11i2.24051

Abstract

Dandruff is a scalp condition characterized by excessive flaking resulting from colonization by the fungus Malassezia furfur. Chemical-based anti-dandruff shampoos frequently result in side effects, including irritation, resistance, and hair discoloration. This study investigates the efficacy of Leucaena leucocephala leaf extract as a natural treatment for dandruff using an in-silico methodology. This research employed molecular modeling and docking assays targeting the 14-alpha lanosterol demethylase enzyme (CYP51), the primary antifungal target in Malassezia furfur. The docking results indicated that the active compounds in Leucaena leucocephala leaves, namely squalene and lupeol, exhibit a competitive binding affinity for CYP51, similar to the mechanism of action of ketoconazole. Squalene engages in polar interactions akin to the natural ligands of CYP51, whereas lupeol demonstrates robust hydrophobic interactions, suggesting its potential as an enzyme inhibitor. The findings indicate that herbal compounds derived from Leucaena leucocephala may serve as a natural alternative for dandruff treatment, presenting a reduced risk of side effects relative to synthetic chemicals. Further in vivo studies are necessary to validate the effectiveness and safety of anti-dandruff topical formulations. Keywords: CYP51, Dandruff, Leucaena leucocephala, Malassezia furfur, Natural antifungal
Molecular Method Optimization to Identify Plasmodium falciparum Multidrug Resistance 1 (pfmdr1) gene as a Predictor of Antimalarial Resistance Sulistyaningsih, Erma; Dewi, Rosita; Rachmania, Sheilla; Kusuma, Irawan Fajar; Kholifaturrohmy, Muhammad Rizqi; Armiyanti, Yunita; Kholifaturrohmah, Sakinatus Sariroh; Andriani, Made Prasanti
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.673-678

Abstract

Several approaches have been designed to control malaria, a disease with high morbidity and mortality, but they face some hurdles. Antimalarial resistance is one of the major challenges for malaria elimination, so the detection of antimalarial resistance is essential. Several molecular markers for antimalarial resistance have been identified, including Plasmodium falciparum multidrug resistance 1 (pfmdr1) gene. This study determined the optimization of molecular techniques to identify the pfmdr1 gene as an antimalarial resistance predictor in Indonesia. The study included patients diagnosed with uncomplicated or severe malaria originating from the health district of Kerom Regency, Papua Province, and Dr. Soebandi Hospital, Jember, East Java Province. Blood samples were collected in the Whatmann filer paper after informed consent. DNA was isolated from dried blood filter paper, and nested PCR was performed using a specific primer, the pfmdr1-A and pfmdr1-B genes. The PCR cycle was optimized based on previous studies. The pfmdr1-A has a similar setting to the earlier study, but the pfmdr1-B had a different optimum setting from the previous study with the annealing temperature of 57oC for nested-1 and 62oC for nested-2. This PCR setting could be used for further examination. The positive results of the amplification indicated the potential for antimalarial resistance in the parasite population. A study on the gene copy number and polymorphism is essential to determine the definitive conclusion on antimalarial resistance.
Co-Authors Afnan, Muhammad Syifaul Desie Dwi Wisudanti Erma Sulistyaningsih Hibatulloh, Muhammad Farhan Irawan Fajar Kusuma Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Nafisa, Tsabita Rosita Dewi Sheilla Rachmania, Sheilla Widanto, Cika Abadi Laily Yunita Armiyanti