Article Per Year (5 Year)
p-Index From 2020 - 2025
3.23
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Dasar Jurnal Ilmu Kedokteran (Journal of Medical Science) Majalah Kedokteran Bandung AL KAUNIYAH Journal of Agromedicine and Medical Sciences Biology, Medicine, & Natural Product Chemistry Elkawnie MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Syntax Literate: Jurnal Ilmiah Indonesia JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jember Medical Journal (JMJ) International Journal of Health and Information System (IJHIS)
Sheilla Rachmania, Sheilla
Department Of Histology, Faculty Of Medicine, University Of Jember, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

Published : 21 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 21 Documents
Search

 1   2   3 
Study of Kidney Repair Mechanism of Cassava Leaf Extract on Gentamicin induced Nephrotoxicity Rena Normasari; Rosita Dewi; Sheilla Rachmania
Journal of Agromedicine and Medical Sciences Vol 3 No 1 (2017)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v3i1.4090

Abstract

Drugs consumption like gentamicin caused damage and decreased of kidney function. Cassava leaf contain lots of micronutrients, such as vitamin C, vitamin A and carotenoids, which also as an antioxidant. The results of the study conducted by Almashuri proved that cassava leaf have high levels of carotenoid compounds. The purpose of this study was to determine the effect of cassava leaf extract on the repairment of renal damage in mice in vivo. This research is true experimental using mice (mus musculus) and were grouped into 5 groups. Two control groups (positive and negative) and 3 treatment groups (cassava leaves 150, 300, and 450 mg / day). Gentamicin is given every day for 14 days and then observed on serum levels of creatinine along with histopathological scores of kidneys’ damage. Statistical analysis showed a significant reduction in serum levels of creatinine (P <0.05) and a significant decrease in renal histopathologic score. This study suggests that cassava leaf extract can repair kidney damage as a result gentamicin-induced nephrotoxicity in mice.Keywords: cassava leaf extract, creatinin, kidney histopathology 
DNA Quality and Quantity on Blood Spot Post Soil and Ultraviolet-C Exposure Muhammad Afiful Jauhani; Sheilla Rachmania
Journal of Agromedicine and Medical Sciences Vol 6 No 3 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i3.19937

Abstract

Bercak darah dapat ditemukan di tempat kejadian perkara (TKP) pada banyak kasus tindak kekerasan. Asam deoksiribonukleat (DNA) pada darah dapat digunakan sebagai data primer untuk proses identifikasi akan tetapi bercak darah di TKP berisiko rusak akibat pajanan tanah dan ultraviolet. Tujuan utama dari penelitian ini adalah untuk mempelajari efek kombinasi dari pajanan sinar ultraviolet-C dan tanah terhadap kualitas dan kuantitas DNA pada bercak darah. Sebanyak 20 gelas berisi 200 gram tanah ditetesi 900µl darah dan diberikan pajanan sinar ultraviolet-C dalam tiga kelompok berdasarkan durasi pajanan yakni satu hari, tiga hari, dan lima hari. Satu kelompok digunakan sebagai kontrol. Ekstraksi DNA dilakukan menggunakan DNAZol dilanjutkan dengan pengukuran spektrofotometri untuk mengetahui kualitas dan kuantitas DNA. Peningkatan konsentrasi DNA dapat diamati yaitu 681,1 pada hari pertama menjadi 1274,7 pada hari ketiga dan mulai menurun menjadi 1090,6 pada hari kelima, sedangkan kemurnian DNA terus menurun secara konstan seiring dengan meningkatnya durasi pajanan. Penelitian ini menunjukkan bahwa pajanan sinar ultraviolet-C dan tanah menyebabkan degradasi molekul DNA menjadi fragmen-fragmen molekul yang lebih kecil sehingga terjadi peningkatan kuantitas DNA yang disertai penurunan kualitas DNA. Penurunan kualitas DNA dapat mempersulit proses identifikasi sehingga isolasi DNA sampel pada tanah terbuka yang terpajan matahari harus dilakukan sesegera mungkin. Kata Kunci: DNA, darah, tanah, ultraviolet C, patologi forensik
The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats Mukhammad Arif Hadi Khoiruddin; Dina Helianti; Muhammad Hasan; Rosita Dewi; Sheilla Rachmania
Journal of Agromedicine and Medical Sciences Vol 8 No 2 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i2.27424

Abstract

Diazinon is an organophosphate pesticide often used by farmer. The residue of diazinon may contaminate agricultural products and if accidentally consumed in a long term, may potentially led to health problems such as kidney disorders. Shallot (Allium cepa) peel was recently proposed to help recover the kidney damage due to its high flavonoid antioxidant content. This study aims to determine the antioxidant effect of shallot peel extract in reducing oxidative stress caused by diazinon with malondialdehyde (MDA) level indicator on the kidney of Wistar rats (Rattus norvegicus). This research was a true experimental with an in-vivo posttest-only control group design. The 28 rats were divided using a simple random sampling technique. The groups were the control group (K0), the diazinon group (K1), SPEE (Shallot Peel Ethanol Extract) groups which are P1 (300 mg/kg BW), P2 (600 mg/kg BW), P3 (900 mg/kg BW), P4 (1200 mg/kg BW) and P5 (2400 mg/kg BW). At the end of the study, the MDA kidney levels was analysed using the TBARS method. Effective doses were found in 600 mg/kg BW/day, 900 mg/kg BW/day, 1200 mg/kg BW/day, and 2400 mg/kg BW/day. The optimal dose of shallot peel extract in this study was 600 mg/kg BW/day. This study showed the effectiveness of shallot peel extract in reducing kidney MDA levels in diazinon-induced Wistar rats. Keywords: shallot peel, diazinon, kidney MDA level
Karakterisasi Peptida Nterm-34 kDa Protein Saliva Nyamuk Aedes aegypti sebagai Kandidat Antigen Biomarker Risiko Transmisi Virus Dengue Secara In Silico Yunita Armiyanti; Nizar Fiska Bayu Agustian; Sheilla Rachmania
Jurnal Ilmu Kedokteran Vol 17, No 1 (2023): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26891/JIK.v17i1.2023.32-40

Abstract

Recently, a new biomarker of mosquito bite has been developed using human antibody response to mosquito salivary protein. One of the proteins that has been tested is salivary protein 34-kDa which has been refined to Nterm-34 kDa peptide. This study aimed to characterize Nterm-34 kDa salivary peptide as a potential antigen biomarker to asses Dengue virus transmission risk. The sequence Nterm-34 kDa was analyzed using several software to predict the characteristic of the peptide. BLASTP from Vectorbase and NCBI wa used to check the specificity of the peptide. IBIVU Praline was used to do multiple sequence alignment. IEDB (Bepipred and Kolaskar & Tongaonkar antigenicity prediction) was used to predict epitopes and antigenic properties, and lastly, ProtParam was used to predict physicochemical properties. The result showed that Nterm-34 kDa salivary peptide is specific to the  Aedes aegypti mosquito at the species level and has low conservation compared to other proteins in Aedes aegypti 34-kDa salivary protein family. Nterm-34 kDa is predicted to be antigenic and one of the epitopes. Nterm-34 kDa salivary peptide is predicted to have a molecular weight of 2,092 kDa, unstable, and hydrophilic. In conclusion, Nterm-34 kDa salivary peptide is predicted to have potential as an antigen biomarker for Dengue virus transmission.
Ekstrak Etanol Kulit Bawang Merah (Allium Cepa L.) Menurunkan Kadar Advanced Oxidation Protein Product (AOPP) Plasma Pada Tikus Wistar Terpapar Diazinon Sheilla Rachmania; Rosita Dewi; Dina Helianti; Ayu Munawaroh
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.966 KB) | DOI: 10.36418/syntax-literate.v7i10.9837

Abstract

Penggunaan diazinon secara ekstensif menimbulkan dampak buruk terutama bagi pekerja di bidang agroindustri. Efek karsinogenik dan toksik diazinon pada organ terjadi melalui jalur stres oksidatif. Flavonoid kuersetin yang terkandung dalam kulit bawang merah (Allium cepa L.) dapat menetralisir kondisi stres oksidatif, terlebih turunan kuersetin, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (BZF), mempunyai efek antioksidan jauh lebih tinggi dibandingkan kuersetin. Studi ini bertujuan mengetahui efek antioksidan ekstrak etanol kulit bawang merah (EKBM) terhadap stres oksidatif pada tikus Wistar terpapar diazinon melalui pengukuran kadar AOPP plasma dan memprediksi target protein BZF. Sebanyak 25 ekor tikus Wistar jantan dibagi dalam kelompok normal, kontrol, dan tiga kelompok perlakuan. Diazinon per oral diberikan dengan dosis 40 mg/kgBB pada 7 hari pertama, dilanjutkan EKBM dengan dosis 600, 900, dan 1200 mg/kgBB pada 7 hari berikutnya. Kadar AOPP plasma diukur dengan metode kolorimetri Witko-Sarsat. Analisis karakterisasi BZF dilakukan dengan pendekatan bioinformatika Swiss Target Prediction. Kadar AOPP pada ketiga kelompok perlakuan secara signifikan lebih rendah dibandingkan kelompok normal (p<0,05) dan antara dosis 600 mg/kg BB dan 900 mg/kgBB menunjukkan perbedaan tidak bermakna (p>0,05). Karakterisasi BZF menghasilkan prediksi target protein, yaitu pada Kanal Potasium Kv1.3 (skor probabilitas >0,65) yang berperan dalam proses inhibisi reaksi oksidasi protein. Sebagai kesimpulan, pada penelitian ini EKBM dengan dosis 600 mg/kgBB paling efektif untuk menetralisir stres oksidatif akibat paparan diazinon yang ditunjukkan dengan penurunan kadar AOPP plasma. BZF diprediksi memiliki target protein dengan probabilitas tertinggi, yaitu pada Kanal Potasium Kv1.3.
Caregiving Burden in Family Caregivers Of Patients With Schizophrenia Post-Pasung in Jember Lusi Padma Sulistianingsih Mata; Inke Kusumastuti; Ida Sri Surani Wiji Astuti; Sheilla Rachmania
Journal of Agromedicine and Medical Sciences Vol 9 No 2 (2023)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v9i2.25247

Abstract

Pasung (restrain) towards people with schizophrenia has yet to recede from being a complex problem in Jember, East Java. One of many aspects of pasung that has not been widely studied is the caregiving burden in the post-pasung period. This research aimed to comprehend the meaning-making of the caregiving phenomenon as experienced by the families of people with schizophrenia during the patients’ post-pasung period in Jember. This research utilizing the interpretive phenomenological analysis (IPA) recruited 11 participants (7 caregivers of people with schizophrenia and 4 community mental health nurses) who were purposively sampled and underwent semistructured interviews from November to December 2019. Two themes emerged in this study namely the experience of the post-pasung caregivers and triangulation with the community mental health nurses which consisted of 8 and 3 subthemes, respectively. Post-pasung caregiving burden experienced by families in this study consisted of subjective, objective, and iatrogenic burdens. Compared with the pasung period, the burden was lighter both for objective and subjective caregiving burden. The most dominating burden is the subjective burdens including apprehensiveness, stigma from the family and the society, and advice from external parties that were against the process of recovery and the objective burden came in the form of the caregiver’s significant health problems. Caregivers made various efforts supported by the provision of accessible and holistic health services as well as interdisciplinary coordination to ease the burden. This study indicates the diversity of the caregiving burden of post-pasung people with schizophrenia. This diversity suggests that interventions aimed at lightening the burden should be carefully selected based on the actual needs of the caregivers. Keywords : caregiving burden, caregivers, pasung, schizophrenia
Specific Sequence Motif of Var Gene as Predictor of Malaria Outcome Erma Sulistyaningsih; Rosita Dewi; Sheilla Rachmania; Irawan Fajar Kusuma; Sahrir Sillehu
Elkawnie: Journal of Islamic Science and Technology Vol 10, No 1 (2024)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v10i1.14808

Abstract

Abstract: The Var gene family encodes for Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP1), a protein responsible for malaria pathogenesis. One of the variants, the var D gene, is hypothesized as a predictor of clinical malaria outcomes. The study aimed to investigate the association between the expression of the var D gene and clinical malaria outcomes. Blood spots on filter paper from uncomplicated and severe malaria patients were collected for DNA and RNA extraction. The RNA was reverse-transcribed into cDNA. DNA and cDNA were amplified by Polymerase Chain Reaction (PCR) technique using specific var D primer, and PCR products were electrophorized in 1% agarose. DNA amplification resulted in double bands of approximately 230 bp and 250 bp in uncomplicated and severe malaria samples. However, the cDNA amplification generated a single band of 230 bp from four out of five severe malaria samples. The existence of band solely in severe malaria transcript suggested its involvement in the pathogenesis of severe malaria. In conclusion, the expression of var D gene-specific sequence can be a potential predictor of severe malaria outcomes.Abstrak: Famili gen var mengkode Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP1), suatu protein yang berperan penting dalam proses patogenesis malaria. Salah satu variannya, gen var D, diduga merupakan prediktor gambaran klinis malaria. Penelitian ini bertujuan untuk mengetahui hubungan antara ekspresi gen var D  dan gambaran klinis malaria. Sampel berupa tetesan darah pada kertas filter dari pasien malaria tanpa komplikasi dan malaria berat dikumpulkan untuk diekstraksi DNA dan RNAnya. RNA selanjutnya di trankripsi reverse menjadi cDNA. DNA dan cDNA diamplifikasi dengan teknik PCR menggunakan primer spesifik var D dan produk PCR dielektroforesis menggunakan agarosa 1 %. Amplifikasi DNA menghasilkan beragam pita berukuran sekitar 230 bp dan 250 bp pada sampel malaria tanpa komplikasi dan malaria berat, tetapi amplifikasi cDNA hanya menunjukkan satu pita berukuran 230 bp pada 4 dari 5 sampel malaria berat. Keberadaan pita hanya pada sampel malaria berat mengindikasikan peran gen ini dalam patogenesis malaria berat. Disimpulkan bahwa gen var D dapat ditemukan pada sampel malaria tanpa komplikasi dan berat, namun ekpresi gen tersebut dapat menjadi prediktor yang potensial  timbulnya manifestasi klinis malaria berat.
Increased of Spleen White Pulp Diameter Post Dbl2β-Pfemp1 Recombinant Protein Injection in Wistar Rats: Pre-Clinical Study for Malaria Vaccine Development Sulistyaningsih, Erma; Sa’diyah, Nindya Audatus; Kusuma, Irawan Fajar; Dewi, Rosita; Rachmania, Sheilla
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 18 No 1 (2024): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v18i1.7451

Abstract

Malaria is a major infectious disease worldwide, and vaccination is essential for disease control. The Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP1) is a potential malaria vaccine candidate due to its involvement in pathogenesis. Injection of DBL2β-PfEMP1 recombinant protein in animal models induces IgG and CD4+ production and inhibits binding with host endothelial receptors. This study aimed to analyze the spleen immune response by measuring the white pulp diameter. The experimental study used Wistar rats (Rattus norvegicus) that were divided into four groups, a control group and three treatment groups, which were injected with 100, 150, and 200 μg of DBL2β-PfEMP1 protein. Injection was done thrice with three-week intervals (days 0, 21, and 42). On day 56, rats were euthanized, and spleens were prepared for histology examination. The white pulp diameter increased along with increasing the dose of protein. The ANOVA test showed a significant difference between groups (p=0.001). The posthoc Bonferroni test showed a significant difference between the control and the 150 and 200 μg groups and the 100 μg and 200 μg groups. In conclusion, the DBL2β-PfEMP1 recombinant protein injection increased the spleen white pulp diameter in Wistar rats, and the 200 μg dose resulted in the highest increase.
Efektivitas Penyuluhan Risiko Preeklampsia Pada Ibu Hamil di Patrang Kabupaten Jember Nurmaida, Eny; Rachmawati, Dwita Aryadina; Rachmania, Sheilla; Putri, Elvia Rahmi Marga; Hakiim, Arsyzilma; Marchianti, Ancah Caesarina Novi; Astuti, Ida Srisurani Wiji; Raharjo, Angga Mardro; Sudarmanto, Yohanes; Kusuma, Irawan Fajar
Jember Medical Journal Vol. 3 No. 1 (2024)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jembermedicaljournal.v3i1.936

Abstract

Angka kematian ibu di Kabupaten Jember dan Jawa Timur masih tinggi.  Terjadipeningkatan kasus kematian ibu pada tahun 2018-2021. Preeklamsia merupakan salah satu penyebab utama kematian ibu hamil. Tindakan preventif seperti edukasi pada ibu hamil mengenai pentingnya pemeriksaan kehamilan pada kehamilan risiko tinggi perlu dilakukan agar tidak terjadi komplikasi. Tujuan penelitian ini adalah untuk mengetahui efektivitas penyuluhan risiko preeklamsia pada ibu hamil di Kecamatan Patrang Kabupaten Jember. Jenis penelitian ini adalah penelitian pra-eksperimental dengan desain penelitian one group pretest-posttest. Instrumen penelitian berupa kuesioner yang berisi 22 pertanyaan mengenai pengetahuan, faktor risiko, gejala dan komplikasi preeklamsia. Besar sampel sebanyak 30 responden (quota sampling). Uji normalitas data menggunakan uji Shapiro Wilk dan uji hipotesis menggunakan uji Wilcoxon Signed Rank. Nilai Sig uji Saphiro Wilk diperoleh 0,033 yang berarti data tidak berdistribusi normal. Dari hasil uji hipotesis diperoleh nilai Asymp. (2-tailed) 0,000 yang menunjukkan terdapat perbedaan yang signifikan antara nilai pretest responden sebelum diberikan penyuluhan dan posttest responden setelah diberikan penyuluhan.
Degree of Hearing Loss and Students' Quality of Life at a State-Owned Extraordinary School in Jember Indonesia Bachtiar Hidayat, Aldy; Rumastika, Nindya Shinta; Rachmania, Sheilla
Journal of Community Medicine and Public Health Research Vol. 5 No. 1 (2024): Journal Community Medicine and Public Health Research
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jcmphr.v5i1.49179

Abstract

Hearing loss is one of the health problems that can be caused by genetic factors, anatomical abnormalities, trauma to the ears and head, ototoxic drugs, and bacterial meningitis. People with hearing loss may have physical, intellectual, and mental limitations that can hinder daily activities and affect quality of life. Extraordinary School (Sekolah Luar Biasa/SLB) is the school that serves school-age children with special needs or those having a physical or/ mental disorder. This study aimed to determine the relationship between the degree of hearing loss and the quality of life of students with hearing disabilities at SLB Negeri Jember, Indonesia. This study used an analytic observational research design. The total number of samples in this study was 23 students who met the inclusion and exclusion criteria, then an audiometer examination and a questionnaire survey were carried out. This study used the Pediatric Quality of Life Inventory (PEDS-QLTM) questionnaire. The statistical test used was the Spearman correlation test. Interpretation of the results was considered to have a significant correlation if the p-value <0.05. The result of statistical analysis using Spearman's rank shows the relationship between the degree of hearing loss and the physical function of students with hearing loss at the SLB Negeri Jember shows a significance value of 0.184. The relationship between the degree of hearing loss and emotional function obtained a result of 0.005. The relationship between the degree of hearing loss and social functioning shows a significance value of 0.561. The relationship between the degree of hearing loss and school functioning showed a significance value of 0.016. The results were considered to have a significant correlation if the p-value <0.05. In conclusion, hearing loss was found not to have significant relationship with physical and social functioning in students with hearing disabilities at SLB Negeri Jember, Indonesia
Co-Authors Aditha Satria Maulana Alami, Eqiel Navadz Akthar Ancah Caesarina Novi Marchianti Andriani, Made Prasanti Angga Mardro Raharjo, Angga Mardro Arsyzilma Hakiim Ayu Munawaroh Ayu Munawaroh Aziz, Ayu Munawaroh Bachtiar Hidayat, Aldy Bintang, Muhammad Farrel Ravidinata Masoga Caesarina, Ancah Cholis Abrori Dina Helianti Dwita Aryadina Rachmawati Erfan Efendi Erma Sulistyaningsih Erma Sulistyaningsih Erma Sulistyaningsih Gianevan, Nicholas Jirezra Hibatullah, Muhammad Naufal Ida Sri Surani Wiji Astuti Ida Srisurani Wiji Astuti Inke Kusumastuti Irawan Fajar Kusuma Irawan Fajar Kusuma Izza Amalia Putri Jauhar Firdaus Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Lusi Padma Sulistianingsih Mata Luthfiyyah, Nur Atikah M. Ali Shodikin Muhammad Afiful Jauhani Muhammad Hasan Mukhammad Arif Hadi Khoiruddin Nizar Fiska Bayu Agustian Nofica, Cheila Nurmaida, Eny Putri, Elvia Rahmi Marga Rahma, Nafisah Hani Asyifah Rena Normasari Rini Riyanti Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rumastika, Nindya Shinta Sahrir Sillehu Sa’diyah, Nindya Audatus Sudarmanto, Yohanes Sulistyaningsih , Erma Sumadi Suryono Suryono Yunita Armiyanti Zahniar, Zahniar