Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Biosaintifika: Journal of Biology & Biology Education
I Komang Tri Yasa Widnyana
Unknown Affiliation
Agriculture, Biological Sciences & Forestry

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Optimizing Canang Flower Waste Extract for Staining Fasciolopsis buski Eggs: An Enhancement of the Kato-Katz Method I Komang Tri Yasa Widnyana; Kadek Indira Maheswari; Putu Sathiya Adi Janendra; Dewa Gede Putra Mahayana; Indra Dwisaputra; Made Bayu Permasutha; Irma Rahmayani; Pasala, Metamalik
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22829

Abstract

Fasciolopsis buski is a parasitic helminth that can infect humans. Diagnosing helminthiasis can be confirmed through fecal testing utilizing the Kato-Katz method.  The Kato-Katz method employs methylene blue staining, which poses environmental hazards due to its carcinogenic characteristics. An alternative to staining is the use of a canang, a common Balinese item used for prayers. The utilized waste canang flowers were Tagetes erecta, Impatiens balsamina (red), and Impatiens balsamina (purple). The research began with an extraction procedure that involved cutting the flowers into small pieces, resulting in approximately 500 grams of fragments. The flower components were macerated in two liters of 96% ethanol for five days. Additionally, cellophane immersion of the extracted findings was performed. The findings indicated that each sample from the three treatment groups (T1, 1%; T2, 2%; and T3, 3%) and the two control groups.  Sub-analysis testing evaluated the quantity of helminth eggs, quantified as eggs per gram of feces via field-of-view observation. The T3 (3%) exhibited results that were not statistically significantly different (P>0.05) from the positive control group. The T3 (3%) test provides the most favorable and optimal results as a substitute for methylene blue in microscopic staining evaluations.
Co-Authors Dewa Gede Putra Mahayana Indra Dwisaputra Irma Rahmayani Kadek Indira Maheswari Made Bayu Permasutha Pasala, Metamalik Putu Sathiya Adi Janendra