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All Journal Indonesian Journal of Tropical and Infectious Disease Biosaintifika: Journal of Biology & Biology Education
Pasala, Metamalik
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

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Optimizing Canang Flower Waste Extract for Staining Fasciolopsis buski Eggs: An Enhancement of the Kato-Katz Method I Komang Tri Yasa Widnyana; Kadek Indira Maheswari; Putu Sathiya Adi Janendra; Dewa Gede Putra Mahayana; Indra Dwisaputra; Made Bayu Permasutha; Irma Rahmayani; Pasala, Metamalik
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22829

Abstract

Fasciolopsis buski is a parasitic helminth that can infect humans. Diagnosing helminthiasis can be confirmed through fecal testing utilizing the Kato-Katz method.  The Kato-Katz method employs methylene blue staining, which poses environmental hazards due to its carcinogenic characteristics. An alternative to staining is the use of a canang, a common Balinese item used for prayers. The utilized waste canang flowers were Tagetes erecta, Impatiens balsamina (red), and Impatiens balsamina (purple). The research began with an extraction procedure that involved cutting the flowers into small pieces, resulting in approximately 500 grams of fragments. The flower components were macerated in two liters of 96% ethanol for five days. Additionally, cellophane immersion of the extracted findings was performed. The findings indicated that each sample from the three treatment groups (T1, 1%; T2, 2%; and T3, 3%) and the two control groups.  Sub-analysis testing evaluated the quantity of helminth eggs, quantified as eggs per gram of feces via field-of-view observation. The T3 (3%) exhibited results that were not statistically significantly different (P>0.05) from the positive control group. The T3 (3%) test provides the most favorable and optimal results as a substitute for methylene blue in microscopic staining evaluations.
Effect of Fetal Bovine Serum Concentration on Detection and Morphological Identification of Blastocystis hominis in vitro Janendra, Putu Sathiya Adi; Sukarma, Kadek Edy; Sarita, Kadek intan Arta; Maheswari, Kadek Indira; Giri, Made Kurnia Widiastuti; Permasutha, Made Bayu; Pasala, Metamalik
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 2 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i2.64829

Abstract

Diarrhea significantly contributes to the high rates of illness and death among young children. Diarrhea can be caused by bacterial infections, viruses, or even parasites. Blastocystis hominis causes parasitic diarrhea, which can be identified by microscopy, culture, and molecular methods. Previous reports have modified the Jones’ culture medium using three different serums, such as human plasma, donkey serum, and horse serum (in Jones’ medium). This research replaces horse serum with fetal bovine serum for detection tests, morphological observation, and diagnosis of B. hominis. The research encompasses five experimental groups, each subjected to varying concentrations of fetal bovine serum: 2%, 10%, 20%, 30%, and 40%. Detection analysis is conducted using the Mc-Nemar test, while the Wilcoxon test is applied to evaluate ordinal data from morphological assessments. Diagnostic tests and metrics such as accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) are performed using MedCalc® software. The findings demonstrate that serum concentrations of 2%, 10%, 20%, and 30% produced effective results in detection tests, morphological identification, and diagnostic evaluations of B. hominis, exhibiting high sensitivity, specificity, PPV, NPV, and accuracy. Fetal bovine serum can be used at a concentration of 2% in a Jones’ medium that has been modified. This depends on the results of detection tests, morphology, and diagnosis.
Co-Authors Dewa Gede Putra Mahayana I Komang Tri Yasa Widnyana Indra Dwisaputra Irma Rahmayani Janendra, Putu Sathiya Adi Kadek Indira Maheswari Made Bayu Permasutha Made Bayu Permasutha Made Kurnia Widiastuti Giri Maheswari, Kadek Indira Putu Sathiya Adi Janendra Sarita, Kadek intan Arta Sukarma, Kadek Edy