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Keanekaragaman Hama Pascapanen pada Komoditas Buah Tropis di Pasar Duman, Lombok Barat Amrul Jihadi; Hari Aditia Pratama, Muhammad; Aulia Rizkika, Gardena
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.6165

Abstract

This study aims to identify post-harvest fruit pests at Duman Market, West Lombok. The research was conducted at Duman Market, Lingsar Subdistrict, West Lombok Regency, West Nusa Tenggara, using a survey method through direct observation of infested fruits. Ten fruit commodities showing pest infestation symptoms were examined, with parameters including pest types and associated symptoms. The results revealed three pest species attacking fruits at Duman Market, namely Noorda albizonalis, Bactrocera spp., and Tirathaba ruptilinea. These findings highlight the importance of post-harvest pest management in maintaining fruit quality and minimizing economic losses, particularly in traditional markets where diverse fruit commodities are traded. The study also provides baseline data for future research and pest control strategies, supporting efforts to strengthen local agribusiness and improve the competitiveness of Indonesian tropical fruits in domestic and international markets.
Respon Pertumbuhan Udang Vaname (Litopenaeus vannamei) terhadap Pakan Ekstrak Daun Jarak Pagar (Jatropha curcas) dengan Dosis Berbeda Ansani Takwin, Bagus; Hari Aditia Pratama, Muhammad
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.7399

Abstract

Pacific white shrimp (Litopenaeus vannamei) is a leading aquaculture commodity in Indonesia, valued for its high nutritional content, rapid growth, and disease resistance. However, pathogenic infections such as Vibrio spp. can cause mortality rates of up to 100% within a day, necessitating strategies to enhance immune function. This study aimed to evaluate the effect of incorporating Jatropha curcas leaf extract into feed on the growth performance and survival of L. vannamei. The experiment was conducted for 60 days using a Completely Randomized Design with four treatments: control (no extract), 1%, 2%, and 3% J. curcas leaf extract, each with three replicates. Parameters measured included absolute weight gain, absolute length gain, specific growth rate (SGR), feed conversion ratio (FCR), and survival rate (SR). Results showed that the 3% treatment (JC3) achieved the highest absolute weight gain (6.37 g), significantly higher (p<0.05) than the control and JC1, but not significantly different from JC2. The highest SGR was also recorded in JC3, while the lowest FCR (1.2) was observed in JC3, which was not significantly different from the control. The improved growth performance with J. curcas supplementation is likely attributed to its bioactive compounds, such as tannins and flavonoids, which possess antibacterial and immunostimulant properties, thereby enhancing shrimp health and feed utilization efficiency. This study recommends a 3% inclusion level of J. curcas leaf extract in feed as an optimal dose to support the growth of L. vannamei.
Karakterisasi Hama Baru Spodoptera Frugiperda Pada Tanaman Jagung (Zea mays L.) Di Lombok Barat Juwanda, Erwin; Supeno, Bambang; Haryanto, Hery; Hari Aditia Pratama, Muhammad
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6062

Abstract

Maize (Zea mays L.) is the main food crop in Indonesia after rice, but is vulnerable to attack by plant pests (OPT), including Spodoptera frugiperda, an invasive insect that attacks corn plants. This research aims to observe the population, level of attack and characteristics of the pest of S. frugiperda in West Lombok Regency. Based on observations, the eggs of this pest are brownish white, round in shape, and have an average size of 0.33 mm × 0.47 mm. The male pupa is reddish brown with dimensions of 14.25 mm × 4.32 mm, while the female pupa is larger, namely 17.53 mm × 5.13 mm. Larvae are found in various instars (2-6) with sizes from 1.53 mm to 35.16 mm, and colors varying from light green to dark brown. Male imagos are smaller than females, with a length of 12.45 mm and a wingspan of 27.73 mm, while female imagos have a size of 14.38 mm and a wingspan of 31.47 mm. The highest population was found at location 3 with 123 larvae, while the lowest population was at location 13 with 1 larva. The highest attack rate occurred at location 7 (50%) and the lowest at location 11 (5%). The observation method includes assessing the level of damage based on the attack intensity score (0–4). This research proposes further studies to understand the distribution and control mechanisms of S. frugiperda in order to minimize its impact on corn cultivation, especially in Nusa Tenggara Barat, Indonesia.
Spider Diversity in Potato Plants Applied with Some Concentrations of Botanical Pesticides Virginia Tobacco Stem Waste Fauzan, Lalu Ahmad; Sarjan, Muhammad; Supeno, Bambang; Hari Aditia Pratama, Muhammad
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6071

Abstract

This study aims to determine the effect of applying various concentrations of botanical pesticides derived from Virginia tobacco stem waste on the diversity of spiders in potato plants. The research was conducted in Sembalun Bumbung Village, Sembalun District, East Lombok Regency, West Nusa Tenggara Province. The method employed was an experimental method with field trials, using a Randomized Block Design (RBD) with six treatments: P0 (control), P1 (abamectin), P2 (Virginia tobacco stem extract 2 ml/L), P3 (Virginia tobacco stem extract 4 ml/L), P4 (Virginia tobacco stem extract 6 ml/L), and P5 (Virginia tobacco stem extract 8 ml/L). The experiment was conducted in three blocks, resulting in 18 experimental units. The results showed that the application of various concentrations of botanical pesticides from Virginia tobacco stem waste did not significantly affect spider diversity in potato plants. However, the P3 treatment (Virginia tobacco stem extract 4 ml/L) exhibited a higher tendency in population, diversity index, abundance index, and dominance index compared to other treatments.
Penampilan Karakter Agronomi Beberapa Genotipe Mutan Padi (Oryza Sativa L.) Baas Selem Generasi Kedua (M2) Hasil Induksi Mutasi Faihrorrozy; Wayan Sri Suliartini, Ni; Ketut Ngawit, I; Hari Aditia Pratama, Muhammad
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6080

Abstract

This study aims to determine the differences in the appearance of agronomic characters of several genotypes of rice mutants (Oryza Sativa L.) The second generation Baas Selem (M2) is the result of mutation induction. This research was conducted in June 2021 - October 2021 in Nyiur Lembang Village, Narmada District, West Lombok Regency. The methods used were group random design (RAK) and Augmented Design with the treatment used, namely 24 genotypes of second generation Baas Selem rice seeds (M2) with control plants. The control plant repeated 3 times, while the mutant plant was 1 time, in its application using an augmented design. The data obtained were analyzed using ANOVA and BNT at the level of 5%. The results showed that Baas Selem rice mutants differed markedly in all observed agronomic characters, from plant height, flag leaf area, total number of tillers, number of productive tillers, number of grain contained, number of empty grains, weight of grain contained, weight of empty grain, weight of grain 100 seeds, flowering age of harvest age and panicle length.
Potensi Anti Xanthomonas dari Bakteri Rizosfer Tanaman Kankung Pagar (Ipomoea carnea) Asmayani, Nurmaliana; Wahyu Setyaningrum, Tri; Ngurah Nara Kusuma, A.A.; Indah Julisaniah, Nur; Hari Aditia Pratama, Muhammad; Qoriasmadillah, Wanda; Faturrahman
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6159

Abstract

The plant pathogenic bacterium Xanthomonas campestris is a Gram-negative, rod-shaped bacterium that causes damage to plant leaves. This particular disease can result in significant economic losses and a reduction in the quality of agricultural products. An alternative method of dealing with this bacteria is to utilise the rhizosphere of kale (Ipomoea carnea) as a biological control. The objective of this study was to ascertain the antibacterial potential of the rhizosphere bacteria of kale (Ipomoea carnea) against the Xanthomonas campestris bacteria and to identify isolates with enhanced antibacterial efficacy against Xanthomonas campestris using the well diffusion method. A total of 13 bacterial isolates were obtained from the rhizosphere of kale, exhibiting distinctive round colonies, flat edges, and a white or cream colouration. A total of 12 gram-negative isolates and one gram-positive isolate were identified. The highest inhibitory activity against X. campestris was demonstrated by isolates R1, R3, and R11, with an average inhibitory zone diameter of 20.01 mm, 20.79 mm, and 20.57 mm, respectively. These values fell into the very strong inhibition category.