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All Journal Jurnal Akuakultur Indonesia JCES (Journal of Character Education Society) Jurnal Ilmu Kelautan Kepulauan Jurnal Pengabdian Magister Pendidikan IPA Journal of Aquaculture and Fish Health MARLIN : Marine and Fisheries Science Technology Journal Journal of Microbiology, Biotechnology and Conservation
Bagus Ansani Takwin
Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology

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DEVELOPMENT ENTERPRENEURSHIP THROUGH INOVATION OF "CILOK-GURITA (Octopus sp.)" AS A NUTRIOUS FOOD Bagus Ansani Takwin; Hasyiati Aini; Fajrianti Dwi Kurnia; Husnul Ayu Juniarti; Dimas Putra Perdana; Salnida Yuniarti Lumbessy
JCES (Journal of Character Education Society) Vol 4, No 2 (2021): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jces.v4i2.4154

Abstract

Abstrak: Cilok yang beredar di masyarakat selama ini adalah cilok yang berbahan dasar daging, akan tetapi harga daging tergolong mahal, sehingga perlunya inovasi baru berupa cilok yang berbahan dasar murah namun memiliki nilai gizi yang tinggi. Cilok gurita (Octopus sp) kaya gizi merupakan solusi yang dapat dikembangkan sebagai keterbaruan inovasi pangan dimasa sekarang ini. Tujuan kegiatan ini adalah untuk menciptakan peluang usaha mandiri melalui produksi cilok gurita yang sehat dan mempunyai nilai gizi yang tinggi bagi semua golongan masyarakat. Metode yang digunakan adalah praktek langsung pembuatan cilok gurita dan menganalisis kelayakan usahanya. Produksi cilok gurita meliputi kegiatan pembuatan produk, pengemasan, dan juga pemasaran kepada konsumen. Cilok Gurita ini terdiri atas berbagai rasa dengan harga jual per porsi (1 mika berisikan 6 pcs) adalah Rp. 5.000,-. Strategi pemasaran yang digunakan adalah secara daring melalui media social whatsapp. Hasil perhitungan cash flow menunjukan bahwa usaha cilok gurita akan mengalami kenaikan kas sebesar Rp.50.000 ketika penjualan mencapai 50 porsi. Usaha cilok gurita ini layak untuk bersaing di pasaran karena penjualan dalam kurun waktu 1 bulan sudah dapat melebihi BEP.Abstract: The cilok that has been circulating in the community so far has been the cilok made from meat, but the price of meat is quite expensive, so there is a need for new innovations in the form of cilok made from cheap but high nutritional value. Nutrient-rich Cilok Octopus (Octopus sp) is a solution that can be developed as a novelty in today's food innovations. The purpose of this activity is to create independent business opportunities through the production of healthy octopus peelings that have high nutritional value for all groups of society. The method used is direct practice of making octopus peel and analyzing the feasibility of its business. The production of cilok octopus includes product manufacturing, packaging, and marketing to consumers. Cilok Octopus consists of various flavors with a selling price per serving (1 mica contains 6 pcs) is Rp. 5,000, -. The marketing strategy used is online via whatsapp social media. The results of the cash flow calculation show that the octopus peel business will experience an increase in cash of IDR 50,000 when sales reach 50 portions. This octopus peel business is feasible to compete in the market because sales within 1 month can exceed BEP.
Program RPL sebagai Upaya Memenuhi Kebutuhan Sayuran pada Masa Pandemi Covid-19 di Area Perumahan dengan Luas Pekarangan Terbatas I Dewa Made Alit Karyawan; Rahmat Wahyudi; Bagus Ansani Takwin; Iwan Kurniawan; Yusmihad Hamzani; Handika Restu Fauzi; Supiandi Supiandi; Desi Melanisia; Sulastri Sulastri; Nita Nurwahdania; Siti Nurtadahlia
Jurnal Pengabdian Magister Pendidikan IPA Vol 4 No 3 (2021)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (868.655 KB) | DOI: 10.29303/jpmpi.v4i3.928

Abstract

Selama pandemi Covid-19, aktivitas di luar rumah dibatasi. Namun pembatasan ini tidak boleh menghentikan produktivitas masyarakat dalam memenuhi kebutuhan pangannya. Terkait hal tersebut, mahasiswa KKN-Unram menawarkan solusi kepada masyarakat dengan memanfaatkan waktu di rumah. Program yang ditawarkan adalah Rumah Pangan Lestari (RPL) yaitu berkebun sayur di pekarangan rumah dengan menggunakan botol bekas, styrofoam, gelas plastik dan alat sederhana. Tujuannya untuk mengedukasi masyarakat agar dapat memanfaatkan ruang kosong di pekarangan rumah untuk dijadikan tempat bercocok tanam sayuran, dengan menggunakan sistem hidroponik. Program ini dilaksanakan dalam dua kegiatan yaitu pembuatan kebun contoh dan sosialisasi kebun sayur pada lahan terbatas. Metode berkebun yang diterapkan adalah metode hidroponik yang ditempatkan di dalam screen house dan dengan media tanah. Kebun contoh digunakan sebagai tempat pelaksanaan materi sosialisasi kepada masyarakat. Sosialisasi menekankan bagaimana menanam sayuran di pekarangan sempit dengan menggunakan botol bekas dan kemasan berbahan styrofoam. Program sesuai rencana, karena keseriusan mahasiswa dan dukungan masyarakat. Mereka tertarik dengan program yang ditawarkan mahasiswa KKN karena menggunakan alat dan bahan yang mudah didapat. Masyarakat sangat antusias datang dan berdiskusi dalam kegiatan sosialisasi dan pelatihan di taman percontohan. Sayuran di kebun percontohan sudah bisa dipanen setelah 40 hari dan hasilnya dibagikan kepada masyarakat sekitar lokasi pelaksanaan program.
Pengaruh pemberian ekstrak daun jarak pagar (Jatropa curcas L.) terhadap sistem imun udang vaname (Litopenaeus vannamei) yang diinfeksi bakteri Vibrio harveyi Bagus Ansani Takwin; Dewi Nur’aeni Setyowaty; Fariq Azhar
Jurnal Ilmu Kelautan Kepulauan Vol 5, No 2 (2022): Jurnal Ilmu Kelautan Kepulauan
Publisher : Fakultas Perikanan dan Kelautan. Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/jikk.v5i2.5483

Abstract

Utilization of Jatropha Leaf (Jatropa curcas) Extract as Immune System Enhancer of Vannamei Shrimp (Litopenaeus vannamei) Infected with Vibrio parahaemolyticus Bagus Ansani Takwin; Dimas Putra Perdana; Muhaemi Muktiniati; Fariq Azhar
Journal of Aquaculture and Fish Health Vol. 12 No. 1 (2023): JAFH Vol. 12 No. 1 February 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i1.29898

Abstract

The problem arises in vanamei shrimp cultivation is the emergence of diseases, such as vibriosis caused by Vibrio parahaemolyticus. Jatropha leaf extract can be used as an immunostimulant because it contains bioactive compounds namely flavonoid, tannins, saponins, and alkaloids. This research used a completely randomized design (CRD) with 5 treatments with 3 replications. The treatments used were; P1 (feed without extract and bacterial infection); P2 (feed without extract and NaCl injection); P3 : Feed + 1% + bacterial infection; P4 : Feed + 2% extract from feed and bacterial infection, P5 : Feed + 3% extract of feed and bacterial infection. Based on the results of the study, the administration of Jatropha leaf extract was able to increase the highest SR value of P2 by 88.3%, the highest THC value of P5 at 24.46 x 106 cells/ml, DHC values ​​such as hyaline cells ranged from 36.67-71%, granular cells ranged from 17-38.57%, and semigranular cells ranged from 12-24.67%, the highest AF value in P5 was 77.25%, and the lowest TBC value was at P2 of 6 x 108 CFU/ml, the lowest TVC value was at P2 of 0.56 x 106 CFU/ml. The higher the dose of extract given, the higher the THC and AF, besides that, Jatropha leaves were also able to increase the SR value and suppress the growth of total bacteria and total Vibrio in shrimp intestines.
The potential of bacteriophage for controlling Vibrio parahaemolyticus as in-vitro Ansani Takwin, Bagus; Wahjuningrum, Dinamella; Widanarni, Widanarni; Nasrullah, Hasan
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.122-133

Abstract

Vibrio parahaemolyticus infection is a major threat to Pacific white shrimp aquaculture, causing significant shrimp mortality. Bacteriophages (phages) provide a promising antibiotic alternative due to their specificity towards specific bacteria. This research includes essential steps isolating phages from shrimp pond water and characterizing them, including plaque morphology, spot tests, phage density evaluations, host range assessments, and in vitro studies targeting V. parahaemolyticus. In-vitro tests encompassed six treatments, each with three replicates: negative control (K-), positive control (K+), antibiotic control, phage density of 107 (FB7), phage density of 108 (FB8), and phage density of FB109 (FB9). Phages were successfully isolated from shrimp pond water in Banyuwangi, indicated by plaque formation on double-layer agar media. These phages exhibited morphological characteristics, featuring small, round plaques (d= 0.02-0.5 ± 0.08 cm) with flat, wavy edges and clear plaque. Phage plaques were amplified with an average density of 3.5×109 PFU/mL. Host range tests revealed that only V. parahaemolyticus Situbondo was susceptible to the isolated phages, while five other bacterial strains were not. In bacterial inhibition tests, treatments with different phage densities significantly outperformed the negative control, media control, and antibiotic control. However, the antibiotic control performed better than phage density treatments (0.61 ± 0.01). All phage density treatments effectively reduced V. parahaemolyticus density compared to the negative control, with the most favorable results observed in the FB9 treatment at a density of 109 PFU/mL. Keywords: Aquaculture, antibiotic, bacteriophage, vannamei shrimp, Vibrio parahaemolyticus ABSTRAK Penyakit akibat infeksi Vibrio parahaemolyticus menjadi perhatian utama dalam budidaya udang vaname saat ini karena dapat menyebabkan kematian massal pada udang. Penggunaan bakteriofage (fage) dapat menjadi alternatif solusi untuk menggantikan antibiotik, karena fage bersifat spesifik pada bakteri tertentu. Penelitian ini meliputi beberapa tahapan, yaitu isolasi fage dari beberapa sumber air tambak, karakterisasi fage meliputi morfologi plak fage, spot test, dan uji kisaran inang, serta uji in vitro penghambatan V. parahaemolyticus oleh fage. Uji in vitro dilakukan dengan enam perlakuan dan masing masing tiga ulangan yaitu kontrol negatif (K-), kontrol positif (K+), kontrol antibiotik, kepadatan fage 107 (FB7), kepadatan fage 108 (FB8), dan kepadatan fage 109 (FB9). Penelitian ini berhasil mengisolasi fage yang bersumber dari air tambak udang di Banyuwangi ditandai dengan terbentuknya plak pada media double layer agar, dengan ciri morfologisnya berbentuk titik-titik dan bulat besar (d= 0,02 ± 0,08 cm), tepian rata dan bergelombang serta memiliki warna plak yang bening. Plak fage dapat diperbanyak dengan densitas fage rata-rata 3,5×109 PFU/mL. Untuk uji kisaran inang menggunakan 6 jenis bakteri dengan sumber dan strain yang berbeda, menunjukkan bahwa hanya bakteri V. parahaemolyticus Situbondo yang dapat lisis oleh fage hasil isolasi, sedangkan lima jenis bakteri lainnya tidak. Pada daya hambat bakteri, perlakuan dengan penambahan pada setiap densitas fage berbeda nyata dibandingkan dengan kontrol negatif, kontrol media dan kontrol antibiotik, walaupun kontrol antibiotik lebih baik dari perlakuan densitas fage (0,61 ± 0,01). Semua perlakuan kepadatan fage mampu mengurangi kepadatan V. parahaemolyticus dibandingkan dengan perlakuan kontrol negatif. Hasil terbaik terdapat pada perlakuan FB9 yaitu 109 PFU/mL. Kata kunci: akuakultur, antibiotik, bakteriofage, udang vaname, Vibrio parahaemolyticus
Respon Pertumbuhan Udang Vaname (Litopenaeus vannamei) terhadap Pakan Ekstrak Daun Jarak Pagar (Jatropha curcas) dengan Dosis Berbeda Ansani Takwin, Bagus; Hari Aditia Pratama, Muhammad
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.7399

Abstract

Pacific white shrimp (Litopenaeus vannamei) is a leading aquaculture commodity in Indonesia, valued for its high nutritional content, rapid growth, and disease resistance. However, pathogenic infections such as Vibrio spp. can cause mortality rates of up to 100% within a day, necessitating strategies to enhance immune function. This study aimed to evaluate the effect of incorporating Jatropha curcas leaf extract into feed on the growth performance and survival of L. vannamei. The experiment was conducted for 60 days using a Completely Randomized Design with four treatments: control (no extract), 1%, 2%, and 3% J. curcas leaf extract, each with three replicates. Parameters measured included absolute weight gain, absolute length gain, specific growth rate (SGR), feed conversion ratio (FCR), and survival rate (SR). Results showed that the 3% treatment (JC3) achieved the highest absolute weight gain (6.37 g), significantly higher (p<0.05) than the control and JC1, but not significantly different from JC2. The highest SGR was also recorded in JC3, while the lowest FCR (1.2) was observed in JC3, which was not significantly different from the control. The improved growth performance with J. curcas supplementation is likely attributed to its bioactive compounds, such as tannins and flavonoids, which possess antibacterial and immunostimulant properties, thereby enhancing shrimp health and feed utilization efficiency. This study recommends a 3% inclusion level of J. curcas leaf extract in feed as an optimal dose to support the growth of L. vannamei.
Effect of Bacteriophage Therapy on the Hemocyte Profile of Whiteleg Shrimp (Litopenaeus vannamei) Infected with Acute Hepatopancreatic Necrosis Disease Takwin, Bagus Ansani
MARLIN Vol 6, No 2 (2025): Agustus 2025
Publisher : Politeknik Kelautan dan Perikanan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/marlin.V6.I2.2025.115-130

Abstract

Acute Hepatopancreatic Necrosis Disease (AHPND), caused by Vibrio parahaemolyticus, poses a major threat to the cultivation of Pacific white shrimp (Litopenaeus vannamei) due to its high mortality rate. This study aimed to evaluate the effectiveness of bacteriophage therapy on the non-specific immune response of shrimp, assessed through Differential Hemocyte Count (DHC) analysis. The research was conducted over six months using a Completely Randomized Design with seven treatment groups, including positive control (KP), negative control (KN), antibiotic treatment (KA), and three bacteriophage dosages: 10⁷ PFU/mL (FB7), 10⁸ PFU/mL (FB8), and 10⁹ PFU/mL (FB9), all challenged with V. parahaemolyticus at 10⁵ CFU/mL. DHC observations were carried out on days 0, 1, 3, and 7 post-treatment. The results indicated that the bacteriophage-treated group, particularly FB9 (10⁹ PFU/mL), maintained a hyaline cell proportion of 50–57% through day 7, similar to the immune profile of the negative control. In contrast, the positive control exhibited a marked decrease in hyaline cells and an increase in granular cells, indicating an immune response to infection. Bacteriophage application, especially at high dosage (FB9), effectively stimulated the shrimp's immune response by preserving hyaline cell levels, making it a promising eco-friendly alternative for AHPND control.
Co-Authors Anak Agung Gede Sugianthara Desi Melanisia Dewi Nur’aeni Setyowaty Dimas Putra Perdana Dimas Putra Perdana Dinamella Wahjuningrum Fajrianti Dwi Kurnia Fariq Azhar Fariq Azhar Handika Restu Fauzi Hari Aditia Pratama, Muhammad Hasyiati Aini Husnul Ayu Juniarti Karyawan, I Dewa Made Alit Lumbessy, Salnida Yuniarti Muhaemi Muktiniati Nasrullah, Hasan Nita Nurwahdania Siti Nurtadahlia Sulastri Sulastri Supiandi Supiandi Wahyudi Rahmat WIDANARNI WIDANARNI Yusmihad Hamzani