Article Per Year (5 Year)
p-Index From 2021 - 2026
0.562
P-Index
This Author published in this journals
All Journal ACTA VETERINARIA INDONESIANA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Mochammad Aqilah Herdiansyah
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Veterinary

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
DINAMIKA POPULASI DAN IDENTIFIKASI MOLEKULER Vibrio spp. PADA AIR PEMELIHARAAN PENYU LEKANG (Lepidochelys olivacea) DI Turtle Conservation and Education Center (TCEC) SERANGAN BALI: Bahasa Indonesia Ni Kadek Wini Ewintiani; I Wayan Rosiana; Wiradana, Putu Angga Wiradana; I Gede Widhiantara; Muhammad Khaliim Jati Kusala; Mochammad Aqilah Herdiansyah; Putu Angga
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.157–166

Abstract

Salah satu bakteri yang dapat mencemari air kolam penyu yaitu bakteri dari Genus Vibrio yang termasuk dalam patogen oportunistik biota perairan. Penyu lekang yang merupakan hasil penyelamatan di alam liar memiliki kerentanan terinfeksi bakteri patogen karena munculnya perlukaan pada permukaan tubuhnya. Tujuan dari penelitian ini adalah untuk mengetahui dinamika populasi dan identifikasi molekuler Vibrio spp pada air pemeliharaan Penyu Lekang (Lepidochelys olivacea) di TCEC Serangan Bali. Pengambilan sampel air dilakukan secara komposit pada tiga kolam yang berbeda. Pengukuran kelimpahan bakteri meliputi total bacaterial count (TBC), Total Vibrio Count (TVC), Total yellow colony (TYC), dan total green colony (TGC) pada mdia pemeliharaan penyu lekang yang dilakukan tiga kali (hari ke-0, 15, dan 30) dengan pengenceran berseri metode cawan sebar untuk dihitung koloni yang tumbuh. Isolat bakteri Vibrio spp. yang berhasil tumbuh dikonfirmasi jenisnya secara molekuler dengan metode PCR. Hasil penelitian menunjukkan nilai TBC pada air kolam pemeliharaan penyu lekang selama penelitian berfluktuasi. TBC tertinggi yaitu 63,02 log CFU/ml pada hari ke-15 di kolam 2 dan TBC terendah yaitu 4,30 log CFU/ml pada hari ke-30 di kolam 3. Nilai TVC tertinggi yaitu 43,27 log CFU/ml pada hari ke-15 di kolam 2 dan terendah yaitu 9,37 log CFU/ ml pada hari ke-0 di kolam 2. Hasil TGC tertinggi yaitu 7,64 log CFU/ml pada kolam 1 hari ke-0 dan terendah yaitu 4,77 log CFU/ ml pada kolam 3 hari ke-30. Hasil TYC tertinggi yaitu 38,08 log CFU/ml pada kolam 2 hari ke-15 dan terendah yaitu 4,39 log CFU/ ml pada kolam 1 hari ke-0. Hasil elektroforesis menunjukkan bahwa gen 16S rRNA pada DNA bakteri Vibrio berhasil diamplifikasi dan ditunjukkan dengan pita yang berukuran kurang lebih 1416 bp. Teridentifikasi satu isolat bakteri Vibrio parahaemolyticus dari hasil penelitian ini.
ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
Co-Authors I Gede Widhiantara I Made Wisnu Adhi Putra I Wayan Rosiana Muhammad Khaliim Jati Kusala Ni Kadek Wini Ewintiani NI KADEK YUNITA SARI Novaria Sari Dewi Panjaitan Permatasari, Anak Agung Ayu Putri Putu Angga Putu Angga Wiradana Sri Puji Astuti Wahyuningsih Wiradana, Putu Angga Wiradana