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All Journal The Indonesian Biomedical Journal Jurnal Kesehatan
Tulandi, Togas
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Biochemistry, Genetics & Molecular Biology Nursing Public Health

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mRNA Expression and DNA Methylation of CXCL16 in Menstrual Blood and Endometrium Tissue of Subjects with Endometriosis and Pelvic Pain Febriyeni, Febriyeni; Hestiantoro, Andon; Tulandi, Togas; Muharam, Muharam; Asmarinah, Asmarinah; Sandra, Ferry
The Indonesian Biomedical Journal Vol 16, No 2 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i2.2958

Abstract

BACKGROUND: The cytokine chemokine ligand 16 (CXCL16) plays an important role in the pathophysiology of endometriosis by regulating the inflammatory response and contributing to the pain-associated endometriosis. Despite this, the impact of epigenetic modifications, such as DNA methylation, on CXCL16 has yet to be fully understood. Therefore, this research was conducted to assess both the mRNA expression and DNA methylation levels of the proinflammatory gene CXCL16 in the endometrium tissue and menstrual blood of patients with and without endometriosis.METHODS: Thirty-five women with and without endometriosis were involved in this research. Subjects' menstrual blood samples were collected using filter paper pads, meanwhile the endometrium tissue were collected by performing biopsy, from which DNA and RNA were extracted. The DNA methylation levels of the CXCL16 were measured using the pyrosequencing method following bisulfite conversion treatment. Meanwhile, the mRNA expression level was measured using the quantitative polymerase chain reaction (qPCR) method and analyzed with the Livak method.RESULTS: The mRNA expression of CXCL16 in menstrual blood of endometriosis subjects was 2.42 times higher compared to control group (p=0.030). Furthermore, the expression of CXCL16 in menstrual blood was identical to that in endometrial tissue (p=0.173). DNA methylation analysis showed that CXCL16 in the menstrual blood of endometriosis subjctes had lower methylation levels compared to controls (p=0.004), indicating hypomethylation.CONCLUSION: Increased mRNA expression and hypomethylation of CXCL16 in the menstrual blood of endometriosis patients could serve as a direct marker for diagnosing endometriosis. However, further study to validate these findings and explore the potential of CXCL16 as a diagnostic tool, and additional research involving larger patient for the cohorts study is necessary.KEYWORDS: CXCL16, DNA methylation, endometrium, menstrual blood, mRNA expression, pain 
Comparison of DNA Extraction Feasibility from Menstrual Blood and Endometrial Tissue in Reproductive-Aged Women Febriyeni; Hestiantoro, Andon; Asmarinah; Tulandi, Togas; Hasnita, Evi; Zuraida; Harnaldo Putra, Billy; Octariyana; Rizki Amanda, Clara; Astria Rishel, RIka
Jurnal Kesehatan - STIKes Prima Nusantara Vol 16 No 2 (2025): Jurnal Kesehatan Volume 16 Nomor 2 Tahun 2025
Publisher : LPPM Universitas Prima Nusantara Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35730/jk.v16i2.1314

Abstract

ABSTRACT The endometrium is a highly vascularized tissue composed of numerous blood vessels, with cells that are formed and destroyed during each menstrual cycle. Menstrual blood, which is shed cyclically from the endometrium in women of reproductive age, contains endometrial tissue or cells. Both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are the primary nucleic acids present in cells with nuclei. In this study, 20 samples of menstrual blood and endometrial tissue were collected from women of reproductive age. Menstrual blood was gathered using specially designed filter paper, and DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, Cat No.: 51304). Endometrial tissue was obtained via biopsy, and DNA was extracted using the Geneaid DNA Isolation Kit (Geneaid, New Taipei, Taiwan). DNA purity and concentration were measured using a Thermo Scientific Nano-Drop microvolume Spectrophotometer. The results indicated an average DNA purity of 1.88 ± 0.09 and a mean concentration of 116.9 ng/µL for menstrual blood, while endometrial tissue had a median DNA purity of 1.92 and a concentration of 192 ng/µL. Statistical analysis revealed no significant difference in DNA purity between menstrual blood and endometrial tissue (p = 0.083), but a significant difference in DNA concentration was observed (p = 0.002). Although there was a slight difference in DNA concentration, both menstrual blood and endometrial tissue samples were equally effective in preserving optimal purity and concentration of DNA molecules. Keywords: Deoxyribonucleic Acid, DNA, Endometrial Tissue, Menstrual Blood, Nucleic Acid.
Co-Authors Andon Hestiantoro Asmarinah Asmarinah Asmarinah Astria Rishel, RIka Billy Harnaldo Putra, Dini Fatmi, Evi Hasnita Febriyeni Febriyeni, Febriyeni Ferry Sandra Muharam, Muharam Octariyana Rizki Amanda, Clara Zuraida