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Putri, Radwa Fathin H
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Bioprospeksi: Eksplorasi Senyawa Aktif Penurun Penderita Stroke dari Organisme Laut Putri, Radwa Fathin H; Yufani, Rofika An; Saniyawati, Fang You Dwi Ayu Shalu; Safitri, Anna
Journal of Tropical Diversity Vol 1 No 1 (2025): Februari
Publisher : Penerbit Brainy Bee

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64283/jotdiverse.2025.1(1):1

Abstract

Stroke is a deadly disease in Indonesia. Meanwhile, marine organisms can contribute to providing active compounds for anti-stroke. The purpose of this review is to provide an overview of stroke sufferers in Indonesia and to identify potential active compounds from marine organisms that can treat or prevent stroke. The writing method was carried out by tracing the literature related to stroke and organisms that produce anti-stroke active compounds through Google Scholar. After collecting data from various literature, the next step was to process the data using simple statistics. Marine organisms that potentially contain anti-stroke active compounds are sea cucumbers (DHA, Docosahexaenoic acid), sea urchins (flavonoids), starfish (flavonoids), and jellyfish (amino acids), while marine organism biomarkers are biological responses of all living things in the sea to pollution and environmental pressures. This review is expected to be the beginning of the development of bioprospecting based on omics: metabarcoding and metagenomics, transcriptomics, proteomics, glycomics, lipidomics, and metabolomics.
Identifikasi Genetik Ikan Nilem dengan Pendekatan DNA Barcoding Dailami, Muhammad; Yuniarti, Ating; Widodo, Maheno Sri; Pratama, Rakhmad Ndaru; Ariyani, Destia Fitri; Siahaan, Marthin Daniel Theo Gratia; Maulana, Antajala Rian; Ramadhan, Surya Dewa; Putri, Radwa Fathin H; Nurcahyani, Shinta Eka; Milansari, Sindi; Mayor, Corazon Ell Merilia Papuani
Journal of Tropical Diversity Vol 1 No 1 (2025): Februari
Publisher : Penerbit Brainy Bee

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64283/jotdiverse.2025.1(1):3

Abstract

Nilem fish (Osteochilus vittatus) is a species of freshwater fish from the Cyprinidae family which has ecological and economic importance in Indonesia, with its natural habitat in rivers, swamps and stagnant waters. Accurate species identification is very important in biological research and fisheries resource management, but morphological identification methods have several limitations. DNA barcoding is a revolutionary molecular technique that uses short DNA sequences to identify and differentiate species with high accuracy. The use of DNA barcoding in identifying freshwater fish in Indonesia has shown significant development, including in a comprehensive study documenting 1,172 native freshwater fish species. The research "Genetic Identification of Nilem Fish using the DNA Barcoding Approach" aims to characterize the genetics of nilem fish, compare the genetic profiles of various populations, and evaluate the effectiveness of DNA barcoding in distinguishing nilem fish from other Osteochilus species, with the hope of contributing to fish conservation and sustainable management efforts nilem in Indonesia. The DNA sequencing results obtained a sequence with a length of 619 base pairs with the composition of Nucleotides A (27.2%), G (17.6%), C (26.4%), T (28.8%). The BLAST results with the NCBI database showed a similarity of 99.84% with the O. vittatus sequence and 99.83% with O. hasseltii. Both species are synonyms with the official name O. vittatus. Phylogenetic analysis is also in line with the BLAST results which show that the sample sequence is in the same clade as the O. vittatus and O. hasseltii sequences. Identification with molecular markers of the COI gene was successfully used in determining the species of the Nilem fish (O. vittatus).
Co-Authors Ariyani, Destia Fitri Ating Yuniarti Maheno Sri Widodo, Maheno Sri Maulana, Antajala Rian Mayor, Corazon Ell Merilia Papuani Milansari, Sindi Muhammad Dailami, Muhammad Nurcahyani, Shinta Eka Pratama, Rakhmad Ndaru Ramadhan, Surya Dewa Safitri, Anna Saniyawati, Fang You Dwi Ayu Shalu Siahaan, Marthin Daniel Theo Gratia Yufani, Rofika An