Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Jurnal Farmasi Sains dan Praktis Borneo Journal of Pharmacy
Wewengkang, Restu Dara
Unknown Affiliation
Immunology & microbiology Medicine & Pharmacology Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
ANTIBIOFILM ACTIVITY OF FIVE NATIVE PLANTS OF KALIMANTAN ISLAND AGAINST Staphylococcus aureus Wibowo, Joko Priyanto; Wahid, Abdul Rahman; Paramitha, Dewi Setya; Wewengkang, Restu Dara; Nurrahmah, Medina
Jurnal Farmasi Sains dan Praktis Vol 11 No 3 (September-December 2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v11i3.12917

Abstract

Infections caused by Staphylococcus aureus are an increasing concern in the healthcare sector, particularly due to the rise of antibiotic-resistant strains. Natural products provide a promising alternative source for discovering new antibacterial and antibiofilm agents. This study aimed to evaluate the antibiofilm activity of five plant species natives to Kalimantan Island against S. aureus. Plant materials were extracted using the ultrasound-assisted extraction (UAE) method with 70% ethanol at 40 kHz and temperature of 30 °C for 30 minutes. Phytochemical screening was performed using standard qualitative tests to detect major secondary metabolite groups, including alkaloids, flavonoids, tannins, saponins, and terpenoids. The antibiofilm assay was carried out using a spectrophotometric microplate method, where biofilm formation was quantified in the presence of plant extracts (500 ppm) compared to negative control (solvent). Data were analyzed statistically using ANOVA (p < 0.05) followed by post-hoc tests to determine significant differences among samples. Among the tested extracts, the gelinggang extract exhibited the strongest antibiofilm effect, inhibiting biofilm formation by 84.39 ± 7.21 %, followed by sangkareho (70.63 ± 7.29 %), kratom (66.54 ± 6.11 %) and matoa (58.36 ± 5.91 %). While, limpasu showed the weakest antibiofilm activity (18.22 ± 1.21 %) in this study. The observed activity may be attributed to the presence of phenolic and flavonoid compounds known to disrupt quorum sensing and biofilm matrix synthesis. These findings highlight the potential of Kalimantan’s native plants, particularly gelinggang, as promising sources of natural antibiofilm agents for combating antibiotic-resistant S. aureus infections.
Antibacterial and Antibiofilm Activity of the Extract and Fractions of Kelakai (Stenochlaena palustris (Burm.F) Bedd) against Methicillin-resistant Staphylococcus aureus Wibowo, Joko Priyanto; Wewengkang, Restu Dara; Nurrahmah, Medina; Fajeriyati, Nurul; Zamzani, Irfan; Jati, Afif Pranaya
Borneo Journal of Pharmacy Vol. 8 No. 4 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i4.8991

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a major concern in healthcare due to its resistance to antibiotics and ability to form biofilms. This study investigated the antibacterial and antibiofilm activity of the extract and fractions of kelakai (Stenochlaena palustris) leaves against MRSA. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids, tannins, and triterpenoids in S. palustris leaves. The ethanolic extract of S. palustris leaves exhibited dose-dependent antibacterial activity against MRSA at concentrations up to 500 ppm. Among the fractions, the n-hexane fraction exhibited antibacterial activity at 500 and 1000 ppm, while the methanolic fraction showed inhibition only at 1000 ppm. The ethyl acetate fraction did not show any inhibition. All fractions and extract demonstrated antibiofilm activity, with the n-hexane fraction exhibiting the strongest activity (91.33 ± 1.52%) at 125 ppm. The crude extract of S. palustris leaves showed the weakest antibiofilm activity (32.66 ± 8.14%). These findings suggest that S. palustris leaves contain compounds with antibacterial and antibiofilm properties against MRSA, with the n-hexane fraction being the most promising. Further studies are needed to isolate and characterize the active compounds responsible for these activities.
Co-Authors Fajeriyati, Nurul Jati, Afif Pranaya Joko Priyanto Wibowo Nurrahmah, Medina Paramitha, Dewi Setya Wahid, Abdul Rahman Zamzani, Irfan