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All Journal Denta: Jurnal Kedokteran Gigi
Yoifah Rizka
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Dentistry

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Chitosan Antibacterial Of Portunus Pelagicus Against Biofilm Porphyromonas Gingivalis Laurencia Isabella Loekito; Yoifah Rizka; Fani Pangabdian
Denta Journal Kedokteran Gigi Vol 12 No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Porphyromonas gingivalis bacteria are found in dental plaques and have the ability to form biofilms. Biofilms are collections of bacterial cells, which are tightly attached to a surface covered by an extracellular polymer matrix released by the bacteria. Periodontitis therapy generally uses tetracycline antibiotics which if used long-term can cause resistance. Chitosan Rajungan crab extract (Portunus pelagicus) has antimicrobial potential which can be used as an alternative therapy. Objective: To determine the antibacterial power of Rajungan crab chitosan extract (Portunus pelagicus) in various concentrations of Porphyromonas gingivalis bacterial biofilms. Method: This research was true experimental laboratorist. This study used Rajungan crab chitosan extract (Portunus pelagicus) to Porphyromonas gingivalis biofilm tested by the test method biofilm. Divided into 5 groups, where each group consisted of 4 samples. The control group consisted of: K- (negative control group), K + (positive control group / tetracycline group), and 3 treatment groups, namely P1 (0.25% Rajungan crab chitosan), P2 (0.5% Rajungan crab chitosan) , P3 (1% Rajungan crab chitosan). Antibacterial power was determined by the value of Optical density in the ELISA Reader. Data analysis using One Way ANOVA followed by LSD test. Result: There were significant differences (p <0.05) seen from the percentage of biofilm inhibition in the negative control group for all groups, the positive control group for the crab crab chitosan extract concentrations of 0.25% and 0.5%. Conclusion: The chitosan crab chitosan extract (Portunus pelagicus) with 1% concentration has the greatest antibacterial effect on the Porphyromonas gingivalis biofilm.
Effect of Hyperbaric Oxygen Therapy Combined with Golden Sea Cucumber (Stichopus hermanii Powder to the Level Of Blood Glucose Diabetic Wistar Rat Induced Porphyromonas gingivalis Rafika Rusydia Darojati; Yoifah Rizka; Syamsulina Revianti
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Diabetes mellitus (DM) is a metabolic disorders characterized by hyperglycemia caused by oxidative stress. Porphyromonas gingivalis (P.g) is a periodontopathogen caused periodontitis exacerbate blood glucose levels (BGL). Hyperbaric oxygen therapy (HBOT) combined with stichopus hermanii (SH) has a role as an antioxidant and antibacterial agent. Purpopse: To analysis effect of HBOT, SH powder and combination of both to the level of blood glucose diabetic Wistar rat induced P.g. Materials and Methods: This study was pre-test-post-test control group design, using 20 male Wistar rat were divided into 5 groups, Group1 (STZ), Group2 (STZ+P.g), Group3 (STZ+P.g+HBOT), Group4 (STZ+P.g+SH powder), Group5 (STZ+P.g+HBO+SH). STZ induction intravenous for 4 days in a row. P.g. induction was exposure 3 times for 4 days. After therapy (7 days), sample blood tail vein of rat were taken for measure BGL by glucometer. Result: Level of blood glucose K1(280.25±88.786)mg/dl, K2(372.25±135.662)mg/dl, K3(249.50±96.652)mg/dl, K4(172.00±82.077)mg/dl, K5(104.75±38.257)mg/dl. Conclusion: Induction of STZ increased the BGL but induction of P.g. cannot exacerbate BGL . HBOT combined with SH powder can reduce BGL in STZ and P.g. induction group.
Co-Authors Fani Pangabdian Laurencia Isabella Loekito Rafika Rusydia Darojati Syamsulina Revianti