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All Journal Denta: Jurnal Kedokteran Gigi
Syamsulina Revianti
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Dentistry

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Degrees of Epithelial Dysplasia in Tounge induced by Candida albicans in Immunosupressed Condition Dwi Andriani; Agni Febrina Pargaputri; Syamsulina Revianti
Denta Journal Kedokteran Gigi Vol 13 No 2 (2019): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Candida albicans is a common opportunistic pathogen that play a significantrole in Oral candidiasis and mucosal carcinogenesis. Several studies founD that Candida sp.associated with dysplastic alteration in the epithelium. Candida present in oral cavity withepithelial change is one of predisposing factor of candida infection and lead to atypia to dysplasia. Immunosupression condition is predisposition factor of oral candidal infection.Objective: To investigate the degree of epithelial dysplasia in tounge induced by Candidaalbicans in immunosuppred condition. Methods: This study was true experimental withposttest-only control group design. Sixteen healthy male Rattus novergicus (Wistar), 12 weeksand 200-250 g weight, were immunosuppressed through oral administration ofdexamethasone and tetracycline for 21 days and induced with C.albicans (ATCC-10231) 1Mc.Farland. The subjects were divided into 4 groups (n=4/group): healthy (HG),immunosuppressed (IG), Immunosupressed+Candida albicans (ICG), and nystatintreatment(NG). The subjects were treated for 14 days, later the rats were euthanized and theirtongue being biopsied. Epithelial dysplasia was subjected to HE examination, observed undera microscope (400x magnification) and statistically analyzed (Kruskall wallis, Mann withey, p<0.05). Result: Epithelial dysplasia of IG was the heaviest than other group. No eptithelialchange were found in group HG. Significant differences existed between HG with othergroups; IG with ICG; ICG with NG (p<0.05). No significant differences was present betweenIG and NG (p > 0.05). Conclusion: There was epithelial change in the tongue both inimmunosupressed condition with or without induction Candida albicans. Degree of epithelialdysplasia in tounge induced by Candida albicans in immunosupressed condition group wasthe heaviest than other group.
Influence of Cellulose Acetate Made From Sea Grass (Enhalus Acoroides) As Surface Coating Acrylic Resin Plates To The Total Colonize Candida albicans Enjel Arjuna Susru Wardana; Puguh Bayu Prabowo; Syamsulina Revianti
Denta Journal Kedokteran Gigi Vol 12 No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: The denture base made of acrylic resin has a porosity and water absorbtion characteristic to cause the deposition of food waste, that problem was known to cause a growth of Candida albicans microorganisms. This problem can be solved by providing a coating (Surface coating) made of natural polymer (cellulose acrtate) of sea sea grass (Enhalus acoroides) mixed with hydrocoloid material so as to provide protection properties. Such protective properties can inhibit the bonding between the residual monomer results of acrylic resins and microorganisms. Objective: To analyze and compare the number of Candida albicans colonies on the acrylic resin plate that has surface coating and no surface coating. Method: Using post test only group design, using 12 samples divided into 2 groups. Group 1 is the control group (Heat cured acrylic plate contaminated with Candida albicans). Group 2 : Treatment group (Heat cured acrylic plate with surface coating made from sea grass Enhalus acoroides then contaminated with Cndida albicans). Calculating the number of colonies from Candida albicans was done by calculating the turbidity of SDB media (Saboraud Dextrose Broth) using UV-VIS Specrofotometer with 560 λ wave. Data analysis using an Independent T-Test. Results: Acrylic resin plates with surface coating showed significant differences in the number of Candida albicans colonies. The average results in the control group obtained an average number of colonies of 1,58 CFU/ml, while in the treatment group as much as 1,42 CFU/ml. Conclusion: The results show that surface coating can decrease the amount of Candida albicans colonies because it can close the microporosity, prevent a water absorption, and prevent the formation of the pellicle.
Effect of Hyperbaric Oxygen Therapy Combined with Golden Sea Cucumber (Stichopus hermanii Powder to the Level Of Blood Glucose Diabetic Wistar Rat Induced Porphyromonas gingivalis Rafika Rusydia Darojati; Yoifah Rizka; Syamsulina Revianti
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Diabetes mellitus (DM) is a metabolic disorders characterized by hyperglycemia caused by oxidative stress. Porphyromonas gingivalis (P.g) is a periodontopathogen caused periodontitis exacerbate blood glucose levels (BGL). Hyperbaric oxygen therapy (HBOT) combined with stichopus hermanii (SH) has a role as an antioxidant and antibacterial agent. Purpopse: To analysis effect of HBOT, SH powder and combination of both to the level of blood glucose diabetic Wistar rat induced P.g. Materials and Methods: This study was pre-test-post-test control group design, using 20 male Wistar rat were divided into 5 groups, Group1 (STZ), Group2 (STZ+P.g), Group3 (STZ+P.g+HBOT), Group4 (STZ+P.g+SH powder), Group5 (STZ+P.g+HBO+SH). STZ induction intravenous for 4 days in a row. P.g. induction was exposure 3 times for 4 days. After therapy (7 days), sample blood tail vein of rat were taken for measure BGL by glucometer. Result: Level of blood glucose K1(280.25±88.786)mg/dl, K2(372.25±135.662)mg/dl, K3(249.50±96.652)mg/dl, K4(172.00±82.077)mg/dl, K5(104.75±38.257)mg/dl. Conclusion: Induction of STZ increased the BGL but induction of P.g. cannot exacerbate BGL . HBOT combined with SH powder can reduce BGL in STZ and P.g. induction group.
Effectivity of the Gold Sea Cucumber Supplementation (Stichopus hermanii) of Catalase Activity in Wistar Rat’s Submandibular with Oral Candidiasis Ajeng Hanun Winny Khairina; Syamsulina Revianti; Nafi’ah
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Oral candidiasis is common opportunistic infection in oral cavity caused by the fungal pathogen Candida albicans and it can develop to malignant such as a candida leukoplakia if it’s affected by cigarette smoke exposure. Purpose: To analysis the effectivity of Stichopus hermanii supplementation of catalase activity in Wistar rats submandibular with oral candidiasis. Materials and Methods: This study was post-test-only control group design, using 35 male Wistar rats were divided into 5 groups, Group1 is negative control, Group2 is positive control, Group3 is treatment control with Stichopus hermanii 0,0225 mg/g BB powder, Group4 is treatment control with Stichopus hermanii 0,045 mg/g BB powder) Group5 is treatment control with Stichopus hermanii 0,09 mg/kg BB powder). Oral candida infection was achieved by spray and cotton swab rolled over all parts of the mouth. After treatment, rats were euthanized and submandibular were biopsied to make histopathology slide for measure activity of enzim catalase with spectrophotometer λ 280 nm. Data were analyzed using One Way ANOVA and Tukey LSD test. Results: There are significantly differences catalase activity between K1&K2, K2&K3, K4&K1, K2&K4, K2&K5. Conclusion: Suplementation of Stichopus hermanii effectiv of catalase activity in wistar rat’s submandibular with Candida albicans A infection.
Efektifitas Ekstrak Nannochloropsis oculata Terhadap Peningkatan Kepadatan Kolagen pada Proses Penyembuhan Alveolar Osteitis. Ajeng Saghita Enggardini; Syamsulina Revianti; Noengki Prameswari
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Alveolar osteitis was one complication after tooth extraction. This conditionremains a common post operative problem that results in severe pain and inhibit sockethealing. Nannochloropsis oculata’s extract contains alkaloids, carotenoids, flavonoids,terpenoids and tocopherols that could accelerate the wound healing process. Purpose: Examine the effectivity of Nannochloropsis oculata extract to the increased density ofcollagen in alveolar osteitis healing process. Materials and Methods: This research designwas post-test only control group design, using 64 male Wistar rats then divided into twomajor groups. Group 1 (irrigation) and group 2 (dressing). In each group contains a negativecontrol group (irrigation PBS or dressing PBS), positive control group (irrigation Saline0,9% or dressing Alvogyl), and the treatment group (irrigation or dressing usedNannochloropsis oculata extract 2,5% and 5%). After treatment on the 3rd and 7th day, ratswere euthanazied than collagen density was measured at histopathology slide used Masson’sTrichom with magnification 100x under light microscope, than it what measured. Data wasanalyzed with Kruskal-Wallis and Mann-Whitney test. Result: There were significantdifferences of collagen density beetwen irrigation treatment groups (0,045), but there werenot significant differences beetwen dressing treatment groups (0,243). Dressing method wasmore effective to increase collagen density than irrigation groups (0,026). Conclusion:Dressing treatment used Nannochloropsis oculata extract concentration 5% was mosteffective to increased the collagen density in alveolar osteitis healing process on 7th days.
Efektivitas Ekstrak Lipid Total Mikroalga (Nannocloropsis Oculata) Dalam Menghambat Pertumbuhan Bakteri Hasil Isolasi Pasca Pencabutan Gigi Rasmi Putri Santoso; Eddy Hermanto; Syamsulina Revianti
Denta Journal Kedokteran Gigi Vol 10 No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Tooth extraction is a treatment procedure that was usually done by a dentist. It has known that the total lipid extraction of microalgae (Nannochloropsis oculata) has antifungal and Gram-positive and Gram-negative antibacterial. Total lipid extract of microalgae 1FAME (fatty acid methyl ester) of total lipid extract microalgae (Nannochloropsis oculata) has the ability to inhibit bacteria. Objective: To know the effectiveness of total lipid extract of microalgae (Nannochloropsis oculata) in inhibiting the growth of bacteria aerobic and anaerobic isolated after tooth extraction. Materials and methods: This research was true experimental research design with post test only control group design. Objects were divided into 10 groups (5 Aerobic and Anaerobic 5), using diffusion method. Negative control were hexane 1%, positive control were amoxicillin 25μg, treatment were total lipid extract of microalgae (Nannochloropsis oculata) with concentration 1.5%, 3% and 6%. Samples were taken using a power point size 45 inserted into the socket former tooth extraction. Samples were incubated and identified, then given 5 treatments (negative control, positive control, microalgae (Nannochloropsis oculata) 1.5%, 3% and 6%. Results: Mann-Whitney showed there are differences between the inhibitory control and treatment groups microalgae (Nannochloropsis oculata). Conclusion: Total lipid extract of microalgae (Nannochloropsis oculata) was able to inhibit bacterial aerobic and anaerobic isolated post-tooth extraction.
Correlation Between Parents Level of Education and Dental and Oral Hygiene in Children with Autism at Mutiara Hati Special School Lita Agustia; Aulia Dwi Maharani; Syamsulina Revianti; Kezia Sepdwiningtyas Santoso
Denta Journal Kedokteran Gigi Vol 15 No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v15i2.3

Abstract

Background: Children with autism have difficulty controlling the movement of tongue that food often cannot perfectly swallowed . Parents play a very important role in maintaining dental and oral hygiene for children with autism. The behavior of children with autism is strongly influenced by the parent’s level of education. Objective: to determine the correlation between parent’s level of education and the level of oral hygiene in children with autism (measured by OHI-S). Methods: This type of research is analytic observational, with a cross sectional approach. Simple random sampling technique was performed. The total sample was 23 students of Mutiara Hati Surabaya Autism Special School. OH Index form by WHO was used to collect the data of dental hygiene and a questionnaire form by RISKESDAS was used to collect data of parent’s level of education. Results: There is a significant correlation between the parent’s level of education and OHI-S in children with autism (<0.05) ,R = -0.49 means the correlation is sufficient. The lower the level of dental and oral hygiene the higher the level of education of their parents. Conclusion: There is a correlation between the parent’s level of education and dental and oral hygiene in children with autism at Mutiara Hati Special School.
Pengaruh Paparan Radiasi Telepon Genggam Terhadap Aktivitas Enzim Katalase Kelenjar Parotis Rattus norvegicus Strain Wistar Yoannita; Isidora Karsini; Syamsulina Revianti
Denta Journal Kedokteran Gigi Vol 10 No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Handheld mobile phones have become a cultural accessory device. Nevertheless, the use mobile phones has given rise to great concern because of possible adverse health effects from exposure to the radiofrequency radiation (RFR) emitted by the device. There have been plenty of researches reportedly establishing the damaging effect of radiofrequency radiation emitted from cell phone on the biological tissues in the body, but only several researches report the effect of handheld mobile phone radiation in oral cavity.Purpose: The aim of this experiment was to examine the difference effect of rat parotid gland catalase activity cause by low and high radiation handheld mobile phone. Material and Methods: This study was true experimental study with post test only control group design. 72male Wistar rats, 3 months old, with body weight 200 grams were used in this experiment, devided into 3 major group. Group 1 was negative contol group, group 2 was radiated by handheld mobile phone with low radiation, and group 3 was radiated by handheld mobile phone with high radiation 1 hour per day until 7 days, 14 days, and 21 days. Parotid gland catalase activity was measured by spectrophotometer λ 240. Data were analyzed by KruskallWallis and Mann-Withney test. Result: Low radiation handheld mobile phone was not altered catalase activity until 7, 14 and 21 days. High radiation handheld mobile phone was not altered catalase activity until 7and 14, but catalase activity was significantly increase at 21 days. Conclusion: Radiation emitted from the high radiadion handheld mobile phone until 21days was able to rise rat parotid gland catalase activity .
Efek Proteksi Ekstrak Etanol Stichopus hermanii Terhadap Jumlah Limfosit pada Tikus yang Terpapar Asap Rokok dan Diinduksi Candida albicans Auliasari Yunanda; Syamsulina Revianti; Isidora Karsini S
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Smoking is associated with oral fungi which can causes oral candidiasis. Stichopus hermanii contains of antioxidant, antifungal and immunostimulating agent. Purpose: To evaluate the protection effect of Stichopus hermanii ethanol extract to lymphocytes amount in Wistar rat with CS exposure and C.albicans infection. Material and Method: This experiment were used post test-only control groups design. We used 42 male Wistar rats and divided into 7 groups. 1st group (saline 0,1mL, fresh air, CMC-Na 0,2%), 2nd group (saline 0,1mL, CS, CMC-Na 0,2%), 3rd group (C.albicans 0,1mL, fresh air, CMC-Na 0,2%), 4th group (C.albicans 0,1mL, CS, CMC-Na 0,2%), 5th group (saline 0,1mL, CS, Stichopus hermanii extract 0,02mg/kgBW), 6th group (C.albicans 0,1mL, fresh air, Stichopus hermanii extract 0,02mg/kgBW), 7th group (C.albicans 0,1 mL, CS, Stichopus hermanii extract 0,02mg/kgBW). Wistar rats has been infected by C.albicans for a week, exposed by CS for 8 weeks, and application of Stichopus hermanii extract for 8 weeks period. Furthermore, Wistar rat has been euthanized after 8 weeks of experiments. The amount of lymphocytes was counted through peripheral blood smear method using Diff-count under light microscope with 1000 times magnification. Data were collected and analyzed using Kruskal-Wallis test and Mann-Whitney test. Result: CS exposure and C.albicans infection group can decreaselymphocytes amount. Suplementation groups with Stichopus hermanii extract can increase lymphocytes amount. Conclusion: Supplementation with Stichopus hermanii extract has protective effect to stimulate lymphocytes proliferation in Wistar rat after CS exposure and C.albicans infection.
Aplikasi Gel Kitosan Berat Molekul Tinggi dan Rendah terhadap Ketebalan Epitel Mukosa pada Proses Penyembuhan Luka Pencabutan Gigi Fransiska Amelia Hartono; Puguh Bayu Prabowo; Syamsulina Revianti
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Acceleration of wound healing after tooth extraction is the most important. One of the tissue repair in wound healing is the reepithelialization. Chitosan is a biomaterial that can be used to accelerate reepithelialization in the wound healing process. Purpose: The aim of this study was to prove the differences between application of chitosan gel high molecular weight and low molecular weight to accelerate the reepithelialization in socket healing after tooth extraction. Material and Methods: The experiment was held the post test only control group design used 24 male wistar rats divided into 3 group. K1 group was control groupwhich was extracted without any treatment, K2 group was extracted and applicated by low molecular weight of chitosan gel, K3 group was extracted and applicated by high molecular weight of chitosan gel. After treatments on the 7 day, all groups of rats were euthanized and the epithelial mucosal thickness was measured under light microscope magnificant 100x. All of the data were analyzed by one way ANOVA and LSD test. Result: This study showed the epithelial thickness of the K2 and K3 groups was significantly higher than K1 group, but there were not significantly different between K2 and K3 group. Conclusion: The effectivity of high molecular and low molecular weight chitosan gel to accelerate reepithelialization in socket healing are similar.
Co-Authors Agni Febrina Pargaputri Ajeng Hanun Winny Khairina Ajeng Saghita Enggardini Aulia Dwi Maharani Auliasari Yunanda Dwi Andriani eddy hermanto Enjel Arjuna Susru Wardana Fransiska Amelia Hartono Isidora Karsini Isidora Karsini S Kezia Sepdwiningtyas Santoso Lita Agustia Nafi’ah Noengki Prameswari Peggie Octavia Halim Puguh Bayu Prabowo Rafika Rusydia Darojati Rasmi Putri Santoso Yoannita Yoifah Rizka Yoifah Rizka Wedarti