Article Per Year (5 Year)
p-Index From 2021 - 2026
1.203
P-Index
This Author published in this journals
All Journal Jurnal Mandala Pharmacon Indonesia ad-Dawaa : Journal of Pharmaceutical Sciences Jurnal Sains dan Kesehatan Journal Pharmaceutical Science and Application (JPSA)
Yanti, Ni Luh Putu Kris Monika
Faculty Of Pharmacy And Health Sciences, Universitas Pendidikan Nasional, 80224, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Profiling Secondary Metabolite of Fresh and Fermented Marigold Flowers and Butterfly Pea Flowers Ni Luh Putu Kris Monika Yanti; Sukrasno; Defri Rizaldy; Arif Al Iman
Ad-Dawaa: Journal of Pharmaceutical Sciences Vol 5 No 2 (2022)
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/djps.v5i2.37714

Abstract

Communities in Bali use "Usada" as an essential plant for traditional medicine. Utilization of a plant as herbal medicine is based on the metabolites contained therein, such as in the marigold flower (Tagetes erecta L.) and butterfly pea flower (Clitoria ternatea L.). Identification of secondary metabolite profiles was carried out by aerobic fermentation. This study aims to identify the secondary metabolite profiles of untreated extracts compared to fermented extracts. Aerobic fermentation is carried out by stacking fresh samples. Extraction by percolation using n-hexane for marigold flowers and methanol for butterfly pea flowers. The extract profile was analyzed by densitometric thin-layer chromatography (TLC). Separation pattern, retention time, and m/z fragmentation with LC-MS/MS. Determined antioxidant activity using the DPPH method, calculating the levels of total phenol (TPC), total flavonoids (TFC), and total carotenoids in each sample. The TLC profiles of control extracts and marigold flower fermentation were similar, However, in the fermented pea flower extract, the intensity of the spots was thicker, and a new compound was formed from the TLC pattern. The TPC of the control and fermented extracts ranged from 0,816 - 1,154 g GAE/100 g extract, the TFC ranged from 0,067 - 0,610 g QE/100 g extract, and the highest total carotenoid was in the fermented marigold flower extract with levels of 215 mg/g simplicia. All control and fermented extracts had antioxidant activity ranging from 46,71 ppm-75,30 ppm. LC-MS/MS analysis showed five compounds from the fermented butterfly pea flower extract, while the control only identified one compound. The fermentation process for each extract experienced changes in the profile of secondary metabolites, starting from UV absorption, spot intensity, compound levels, types of compounds contained, and their antioxidant activity.
Perbandingan Kadar Flavonoid, Fenol, dan Aktivitas Antioksidan pada Kulit Buah Pisang Kepok (Musa acuminata x balbisiana) dengan Menggunakan Metode Ekstraksi Berbeda: Comparison of Flavonoid, Phenol, and Antioxidant Activity Levels in Kepok Banana (Musa acuminata x balbisiana) Peel Using Different Extraction Methods Arif Al Iman; Sukrasno Sukrasno; Defri Rizaldy; Ni Luh Putu Kris Monika Yanti
Jurnal Sains dan Kesehatan Vol. 5 No. 6 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i6.2134

Abstract

Kepok banana peels are rich in flavonoid and phenolic compounds. To obtain the maximum desired compound, the selection of the extraction method used plays an important role. This research aims to determine the kepok banana peel extraction method that is capable of providing the highest levels of flavonoids and phenols, as well as the strongest antioxidant activity. The research began with the preparation of kapok banana peel simplicia, followed by extraction respectively by meseration, reflux, and Ultrasound Assisted Extraction (UAE). Then the levels of flavonoids, phenols and antioxidant activity of the extracts obtained from each method were measured, and ended with statistical analysis. The results of determining the levels of flavonoid extracts showed that the meseration, reflux, and UAE methods each produced levels of 4.782 ± 0.258 g QE/100 g; 5.877 ± 0.196 grams QE/100 grams; and 7.115 ± 0.226 grams QE/100 grams. In determining phenol content, extracts from meseration, reflux, and UAE produced levels of 8.172 ± 0.505 g GAE /100 g respectively; 9.123 ± 0.224 grams GAE /100 grams; 10.104 ± 0.345 grams GAE /100 grams. Meanwhile, the antioxidant activity test showed that the IC50 value of mecerated, refluxed and UAE extracts was 39.493 ± 0.819 ?g/ml; 28.203 ± 0.516 ?g/ml; 23.147 ± 0.314 ?g/ml. The conclusion of this research is that UAE extraction is the best extraction method, with a total flavonoid content of 7.115 ± 0.226 g QE/100 g, a total phenol content of 10.104 ± 0.345 g GAE /100 g, and the strongest antioxidant IC50 of 23.147 ± 0.314 ?g/ml. Keywords:          Antioxidants, Banana Peel, Extraction, Flavonoids, Phenols   Abstrak Kulit pisang kepok kaya akan senyawa flavonoid dan fenolik. Untuk memperoleh senyawa yang diinginkan secara maksimal, maka pemilihan metode ekstraksi yang digunakan menjadi peran penting. Penelitian ini bertujuan untuk menentukan metode ekstraksi kulit pisang kepok yang mampu memberikan kadar flavonoid dan fenol tertinggi, serta aktivitas antioksidan terkuat. Penelitian diawali dengan penyiapan simplisia kulit buah pisang kapok, dilanjutkan dengan ekstraksi masing-masing dengan maserasi, refluks, dan Ultrasound Assisted Extraction (UAE). Kemudian, kadar flavonoid, fenol dan aktivitas antioksidan ekstrak yang diperoleh dari masing-masing metode diukur, dan diakhiri dengan analisis statistik. Hasil penetapan kadar flavonoid ekstrak menunjukkan bahwa metode maserasi, refluks, dan UAE menghasilkan kadar masing-masing sebesar 4,782 ± 0,258 g QE/100 g; 5,877 ± 0,196 g QE/100 g; dan 7,115 ± 0,226 g QE/100 g . Pada penetapan kadar fenol, ekstrak dari maserasi, refluks, dan UAE menghasilkan kadar masing-masing sebesar 8,172 ± 0,505 g GAE /100 g; 9,123 ± 0,224 g GAE /100 g; 10,104 ± 0,345 g GAE /100 g . Sementara, uji aktivitas antioksidan menunjukkan bahwa nilai IC50 ekstrak hasil maserasi, refluks, dan UAE masing-masing sebesar 39,493 ± 0,819 ?g/ml; 28,203 ± 0,516 ?g/ml; 23,147 ± 0,314 ?g/ml. Kesimpulan penelitian ini adalah ekstraksi UAE merupakan metode ekstraksi terbaik yaitu dengan kadar flavonoid total sebesar 7,115 ± 0,226 g QE/100 g, kadar fenol total sebesar 10,104 ± 0,345 g GAE /100 g, dan IC50 antioksidan terkuat sebesar 23,147 ± 0,314 ?g/ml. Kata Kunci:         Antioksidan, Ektraksi, Fenol, Flavonoid, Kulit Buah Pisang Kepok
Formulasi Krim Ekstrak Daun Kumis Kucing (Orthosiphon aristatus L.) dan Daun Cabai Rawit (Capsicum frutescens L.) sebagai Antioksidan dan Antibakteri Yanti, Ni Luh Putu Kris Monika; Unique, I Gusti Ayu Nadia Prasta; Dewi, Putu Indra Cyntia; Putra, I Putu Gede Pramana; Sukratini, Ni Luh; Iman, Arif Al
Jurnal Mandala Pharmacon Indonesia Vol. 10 No. 2 (2024): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v10i2.634

Abstract

Penyakit kulit, mulai dari jerawat hingga psoriasis, merupakan masalah umum yang mempengaruhi banyak individu dalam masyarakat. Masalah ini tidak hanya mereduksi kepercayaan diri tetapi juga berdampak pada kesehatan mental. Reactive Oxygen Species (ROS) menjadi salah satu penyebab utama penyakit kulit, termasuk jerawat, melalui mekanisme peroksidasi lipid dan inflamasi. Resistensi bakteri terhadap antibiotik telah meningkat, menimbulkan tantangan dalam penanganan jerawat. Tujuan penelitian ini adalah mengeksplorasi aktivitas antioksidan dan antibakteri dari ekstrak daun cabai rawit (Capsicum frutescens L.) dan kumis kucing (Orthosiphon aristatus L.) serta membandingkan ekstrak tunggal dan sediaan krimnya. Metode yang dilakukan pada penilitian ini terdiri dari determinasi tanaman, pembuat ekstrak, formulasi sediaan, evaluasi sediaan fisik, uji aktivitas antioksidan, dan uji aktivitas antibakteri. Hasil yang telah diperoleh aktivitas antioksidan ekstrak daun kumis kucing (Orthosiphon aristatus L.) dan daun cabai rawit (Capsicum frutescens L.) berturut-turut adalah 38,95 ppm dan 24,62 ppm yang tergolong aktivitas sangat kuat, sedangkan untuk kedua sediaan krim aktivitas yang kuat ditunjukkan pada formula F3 konsentrasi ekstrak 15% karena memiliku nilai IC50 pada rentang 50-100 ppm. Perolehan nilai KHM (Konsentrasi Hambat Minimum) pada ketiga formula baik krim daun kumis kucing dan krim daun cabai rawit bervariasi terhadap bakteri Staphylococcus epidermidis, dilihat dari pengujian aktivitas antioksidan dan antibakteri pada pengujian antioksidan ekstrak lebih memiliki nilai antioksidan yang baik sedangkan untuk antibakteri sediaan krim memiliki potensi dikembangkan lebih lanjut, karena mampu menghambat pertumbuhan bakteri S. epidermidis.
Identifikasi Profil Sidik Jari (Fingerprint) Fitokimia, Kadar Total Fenol dan Kadar Total Flavonoid pada Ekstrak Daun Kelor (Moringa oleifera L.) Yanti, Ni Luh Putu Kris Monika; Dewi, Ni Made Ayu Sukma; Suryanadi, Ni Kadek Ayu; Anggrayani, Putri Siswati
Jurnal Mandala Pharmacon Indonesia Vol. 11 No. 2 (2025): Jurnal Mandala Pharmacon Indonesia 
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v11i2.1025

Abstract

Indonesia merupakan negara tropis yang kaya akan tanaman obat, salah satunya daun kelor (Moringa oleifera L.) yang dikenal memiliki beragam metabolit sekunder seperti flavonoid, fenol, tanin, saponin, alkaloid, dan steroid/triterpenoid. Penelitian ini bertujuan mengidentifikasi profil fitokimia, total fenol, dan flavonoid daun kelor menggunakan KLT dan spektrofotometri UV-Vis. Setelah determinasi, pembuatan simplisia, dan ekstraksi dengan etanol 96% dan etil asetat, hasilnya menunjukkan simplisia dan ekstrak kelor mengandung metabolit sekunder. Analisis sidik jari fitokimia dengan KLT menunjukkan perbedaan kandungan senyawa berdasarkan asal tanaman, sementara ekstrak etanol 96% terbukti lebih efektif dalam mengekstraksi total fenol dan flavonoid secara signifikan dibandingkan ekstrak etil asetat. Data dianalisis menggunakan uji normalitas Shapiro-Wilk, Independent Sample T-Test, dan Mann-Whitney. Hasil skrining fitokimia menunjukkan bahwa simplisia dan ekstrak daun kelor positif mengandung metabolit sekunder. Analisis fingerprint menggunakan KLT memperlihatkan variasi pola bercak pada ekstrak dari daerah berbeda, yang mengindikasikan adanya perbedaan kandungan senyawa. Kandungan total fenol pada ekstrak etil asetat dan etanol 96% masing-masing 253,12 mg GAE/g dan 278,57 mg GAE/g, serta kadar flavonoid masing-masing 537,4 mg QE/g dan 593,4 mg QE/g. Hasil ini menunjukkan bahwa ekstrak etanol 96% lebih efektif secara signifikan dibandingkan etil asetat dalam mengekstraksi senyawa bioaktif daun kelor.
ANTIBACTERIAL ACTIVITY TEST OF FENNEL LEAVES AND STEMS (Foeniculum vulgare Mill.) AGAINST BACTERIA Cutibacterium acnes AND Staphylococcus epidermidis Yanti, Ni Luh Putu Kris Monika; Dasilva, Madahlena Isaura; Andreani, Putu Ririn; Suardiana, I Kadek
Journal Pharmaceutical Science and Application Vol. 7 No. 2 (2025): Journal Pharmaceutical Science and Application
Publisher : Pharmacy Department, Math and Sciences Faculty, Udayana Univerity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JPSA.2025.v07.i02.p02

Abstract

Background: Acne vulgaris is a common skin problem that often affects adolescents and young adults, caused by the bacteria Cutibacterium acnes and Staphylococcus epidermidis. The use of synthetic antibiotics for acne treatment can lead to resistance; therefore, it is necessary to develop alternative natural ingredients that can act as a companion or complementary therapy, such as fennel plants (Foeniculum vulgare Mill.). Objective: To identify and compare the antibacterial activity of ethanol extracts of fennel leaves and stems against C.acnes and S.epidermidis. Methods: Extraction is carried out by maceration using 96% ethanol. Antibacterial tests were carried out in vitro using disc diffusion methods with concentrations of 50%, 75%, and 100%. Tetracycline 1% is used as a positive control, and DMSO 10% as a negative control. Results: Extracts of fennel leaves and stems exhibited antibacterial activity. The diameter of the inhibition zone increased with increasing extract concentration, with the leaves extract showing greater antibacterial activity than the stems extract. Conclusion: The results of the antibacterial activity test showed that ethanol extracts of leaves and fennel stems were able to inhibit the growth of C. acnes and S. epidermidis, with an average inhibition zone of leaf extract that was more optimal than that of stems.
Co-Authors A.A.I.A. Sica Oktavillariantika Andreani, Putu Ririn Anggrayani, Putri Siswati Arif Al Iman Arif Al Iman Cokorda Istri Sri Arisanti Dasilva, Madahlena Isaura Defri Rizaldy Defri Rizaldy Dewi, Ni Made Ayu Sukma Dewi, Putu Indra Cyntia Iman, Arif Al Ni Putu Uma Sari Dewi Putra, I Putu Gede Pramana Suardiana, I Kadek Sukrasno Sukrasno Sukrasno Sukratini, Ni Luh Suryanadi, Ni Kadek Ayu Unique, I Gusti Ayu Nadia Prasta