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All Journal Buletin Veteriner Udayana
Ni Ketut Suwiti
Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
Immunology & microbiology Public Health Veterinary

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HISTOMORPHOMETRY OF THE SUPERFICIAL PECTORALIS MUSCULAR AND CRANIAL TIBIALIS MUSCULAR OF BALI DUCKS IN THE GROWTH PHASE I Kadek Ari Satria Prayoga; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p11

Abstract

Superficial pectoralis muscle is a chest muscle that is located on the surface and functions in wing movement. Tibialis cranialis muscle is the top muscle in the calf muscle structure, which functions to support the bird's body. This study aims to determine the histomorphometry of the superficial pectoralis muscle and cranial tibial muscle of male and female Bali ducks in the growing phase. This research used 20 Balinese ducks aged 12 weeks. Direct anatomical examination and histological structure with a binocular light microscope. Histomorphometry was measured using the Olympus Cellsens Standard application. Anatomy and histology results were analyzed using descriptive qualitative analysis, and histomorphometry using the ANOVA test with mean estimation. Histological structure of the superficial pectoralis muscle and cranial tibial muscle consists of muscle fibers, fasciculus, endomysium, perimysium and epimysium connective tissue. Histomorphometry of fascicle size, perimysium connective tissue thickness, and superficial pectoralis muscle endomysium were significantly different (P<0.05). Histomorphometry of the size of the fasciculus, perimysium connective tissue and endomysium of the cranial tibial muscle was not significantly different (P>0.05) in different genders. It can be concluded that the superficial pectoralis muscle and cranial tibial muscle of males and females in the growing phase are the same in terms of anatomical structure, but the size of the histological structure is different. Histomorphometry of the superficial pectoralis muscle of male and female Bali ducks is significantly different (P<0.05), but not for the tibialis cranialis muscle. Further research is needed regarding the muscles of Bali ducks at other ages.
STRUCTURE AND MORPHOMETRY OF BALI DUCK PANCREAS IN THE GROWER PHASE I Gde Andhika Putra Pratama; Ni Luh Eka Setiasih; Sri Kayati Widyastuti; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p12

Abstract

The pancreas is part of the digestive system which acts as a digestive aid organ. The pancreas has two functions, namely exocrine and endocrine. This study aims to determine the structure and morphometry of the pancreas of Bali ducks (Anas Sp) of different sexes in the growing phase. This research used 18 Balinese ducks which were divided into 2 groups, namely male and female with 9 each (3 months old). The method for examining anatomical structures is carried out by direct observation and histological structures using a binocular light microscope. Morphometry was measured using calipers for length and width, scales for pancreatic weight, and measuring cups for volume. Histomorphometry was measured using the Olympus cellSens Standard application. The results of the anatomical and histological structure data were analyzed using qualitative descriptive analysis, while the morphometric data used the Independent Sample T-Test. The anatomical structure of the Bali duck pancreas is flat like a lobed tongue and pink in colour, consisting of a capsule, islets of Langerhans, acini, intercalary ducts, intralobular ducts, interlobular ducts, intralobular septa, interlobular septa, veins and arteries. The results of morphometric measurements of Bali duck pancreas showed significant differences (P<0.05) in the weight and volume of the pancreas. Histomorphometric measurements of the area of the islets of Langerhans, the area of the acini, the thickness of the intralobular septa, and the thickness of the interlobular septa showed that they were not significantly different (P>0.05) in both sexes. The anatomical structure and histology of the pancreas of male and female Bali ducks are the same, but there are differences in morphometry
HISTOLOGY AND PROFILE OF AGRANULOCYTE OF BALI CATTLE WITH CONVENTIONAL REARED SYSTEM Putri Utami; Ni Ketut Suwiti; I Nyoman Suartha; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2024 Vol. 16 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i05.p04

Abstract

Bali cattle are one of the livestock that are currently widely bred by farmers in Bali. Generally, Bali cattle are raised conventionally or traditionally and pay less attention to the health of the cattle. This situation can affect the immune system of Bali cattle, especially white blood cells that play a role in the immune system such as lymphocytes and monocytes. This study aims to determine the histomorphometry of white blood cells agranulocytes lymphocytes and monocytes in Bali cattle with conventional reared system. Blood test preparations were taken from 30 cows in Bulian Village, Kubutambahan Subdistrict, Buleleng Regency, Bali. Fixation and staining using MDT IndoReagen®. Measurements were made using the EP View application connected to a microscope with 1000x magnification. Calculation of the number of lymphocytes and monocytes by counting up to 100 white blood cells. The results showed that the histomorphology of lymphocytes has an irregular shape, a thin blue-purplish cytoplasm, with a nucleus that almost fills the cytoplasm. Lymphocytes have an area of 17.56 ± 2.37 µm2, perimeter of 14.7 ± 1.14 µm, and diameter of 4.71 ± 0.33 µm. The histomorphology of monocytes has an irregular shape, the cytoplasm is not granular and the nucleus is found hollow. Monocytes have an area of 22.59±5.09 µm2, perimeter of 16.69±2.37 µm, and diameter of 5.19±0.66 µm. Further research is needed on the histomorphometry of granulocytes: neutrophils, basophils, and eosinophils of Bali cattle.
Co-Authors Anak Agung Sagung Kendran I Gde Andhika Putra Pratama I Kadek Ari Satria Prayoga I Ketut Suatha I Nyoman Suartha Luh Gde Sri Surya Heryani Ni Luh Eka Setiasih Ni Nyoman Werdi Susari Putri Utami Sri Kayati Widyastuti