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All Journal Buletin Veteriner Udayana
I Nyoman Sulabda
Laboratorium Fisiologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia 80234
Immunology & microbiology Public Health Veterinary

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HETEROPHIL-LYMPHOCYTE RATIO IN LAYING HENS VACCINATED WITH NEWCASTLE DISEASE-INFECTIOUS BRONCHITIS Savira Hadi Putri; Anak Agung Sagung Kendran; Gusti Ayu Yuniati Kencana; I Made Damriyasa; Ida Bagus Kade Suardana; I Nyoman Sulabda
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p29

Abstract

Vaccination plays a role in the formation of specific immunity against certain antigens. Stressful conditions in animals can potentially influence post-vaccination immune responses. To be able to monitor this condition, it can be seen using stress indicators in poultry, namely the heterophil-lymphocyte ratio (H/L) value. This research was conducted to determine the value of the heterophil-lymphocyte ratio (H/L) in laying hens vaccinated with ND-IB. The samples used were 10 chicken blood samples taken from 40 chickens randomly during the pre-vaccination period; one week, two weeks, and three weeks post-vaccination. The research was preceded by calculating total leukocytes and leukocyte differential, followed by calculating the heterophil-lymphocyte (H/L) ratio value from the results obtained, then analyzed using the SPSS program. The results of this research show that livestock experienced mild stress in the first week post-vaccination which is thought to have occurred due to the handling carried out. A significant increase in the heterophil-lymphocyte ratio (H/L) value occurred in the second week post-vaccination, this occurred due to the influence of environmental factors in the form of bacterial infections. In the third week post-vaccination, livestock no longer experience stress. Further research needs to be carried out using negative controls to determine the exact cause of the increase in the heterophil-lymphocyte (H/L) ratio.
Co-Authors Anak Agung Sagung Kendran Gusti Ayu Yuniati Kencana I Made Damriyasa Ida Bagus Kade Suardana Savira Hadi Putri