Gusti Ayu Yuniati Kencana
Laboratorium Virologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia

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CANINE PARVOVIRUS AND ESCHERICHIA COLI IN INFECTION MIX BREED DOG Made Ramadhinita Desrianti; Gusti Ayu Yuniati Kencana; I Gusti Ketut Suarjana; I Ketut Berata; I Made Dwinata
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2024 Vol. 16 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i05.p09

Abstract

Canine Parvovirus Disease is an infectious disease that causes death in dogs by Canine Parvovirus (CPV). This disease is fatal and contagious, causing the highest number of deaths in dogs, especially in puppies under six months of age. The aim of this research is to find out how to diagnose diseases caused by Canine parvovirus and other secondary infections. Diagnosis is made by looking at clinical signs, anatomical pathology and histopathology and carrying out a PCR test. A 3 month old male mixed breed dog with clinical signs of weakness, lack of appetite and drinking, vomiting and bloody diarrhea. The anatomical pathological changes observed were brain congestion, the heart apex was blunt, the liver and lungs were hemorrhagic, the spleen was dark red in some parts, and the intestine was hemorrhagic. Histopathologically, changes were found in the animal's organs, heart, lungs, liver, spleen and intestines. Virus test samples from intestines, spleen and heart. The results of the Polymerase Chain Reaction (PCR) test showed a positive result with a length of 900 bp. The bacterial test results from the lung organ samples were positive for Escherichia coli which was marked red on Mc Conkey media. It was concluded that the dog was infected with Canine Parvovirus and Escherichia Coli.
THE INFLUENCE OF ADMINISTERING MENIRAN EXTRACT FOR SEVEN DAYS ON BROILERS BEFORE NEWCASTLE DISEASE VACCINATION I Made Indra Palaguna; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p37

Abstract

Newcastle disease (ND) is a detrimental viral disease that affects broiler chickens. Chickens that contract the virus post-vaccination cause significant losses for farmers. Administering meniran extract is one alternative that can enhance the effectiveness of vaccination in chickens. This study aims to determine the effect of administering meniran extract for 7 days before vaccination on the antibody titer against Newcastle disease. The research subjects used were 30 broiler DOCs (Day-Old Chicks) aged 1 day to 30 days. The research design employed a Completely Randomized Design with 3 treatments. The research procedures included preparation of the cages, animal treatment, vaccination, blood sampling, and ND antibody titer testing. Data analysis was performed using ANOVA and Regression Analysis. The study showed an increase in the average antibody titer in each treatment group. The results of this study indicate that administering meniran extract can increase the antibody titer in chickens vaccinated with the LaSota (active) vaccine in the first week before vaccination, as seen in the average antibody titer value of the treatment group, which increased up to 4.5 HI units log 2. Based on the results of the study, further research is needed with a longer administration period of meniran extract or different doses to observe the increase in antibody titer.
POTENTIAL OF MENIRAN LEAF EXTRACT ON NEWCASTLE DISEASE ANTIBODY TITER IN BROILERS Citra Widiawati; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana; Ni Luh Eka Setiasih; Tjokorda Sari Nindhia; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p15

Abstract

Newcastle Disease (ND) is one of the infectious diseases that easily attack broilers. Vaccinations carried out to optimize chicken immunity often get unsatisfactory results, so green meniran (Phyllanthus niruri Linn.) is needed as an immunostimulator. Green meniran leaf extract contains flavonoid compounds as the main component to trigger T cells to help B cells to produce antibodies. This study aims to determine the effect of meniran leaf extract (Phyllanthus niruri Linn.) on booster vaccinated broilers on ND antibody titer. The research design used was a complete randomized design in a nested pattern with a total of 30 broilers divided into three treatment groups, namely 10 broilers not given the ND La Sota booster vaccine and not given meniran leaf extract but given a placebo (K-), 10 broilers given the ND La Sota booster vaccine without meniran leaf extract (K+), and 10 broilers given the ND La Sota booster vaccine and meniran leaf extract in drinking water for seven days before and 14 days after vaccination (P). The samples used were one day before booster vaccination (9-day-old broilers), one week after booster vaccination (17-day-old broilers) and two weeks after booster vaccination (24-day-old broilers). Serum obtained was examined serologically by hemagglutination inhibition (HI) test. The data obtained were analyzed statistically Analysis of Variance (Anova) with a significant level of 5% and regression analysis using SPSS (Statistical Product and Service Solution) software. The results showed that in the administration of meniran leaf extract(Phyllanthus niruri Linn.) and the effect of sampling time after ND booster vaccination had an increase in ND antibody titer which was significantly different (P < 0.05) in treatment P with a mean of 2.8; 4.3; 7.1 and a total mean of 4.7 (titer in HI log 2). Regression analysis showed that the P treatment had the highest increase in the third week after ND booster vaccination compared to the K- and K+ treatments.
SUSPECTED OF TRANSMISSIBLE GASTROENTERITIS AND COLIBACILLOSIS IN PIGLET AT TEGAL SUCI VILLAGE FARM, SEBATU DISTRICT, GIANYAR REGENCY, BALI Shafira Laili Aulia; I Ketut Suarjana; Ida Bagus Oka Winaya; Gusti Ayu Yuniati Kencana; I Made Dwinata
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p17

Abstract

The case pig originated from a farm in Tegal Suci Village, Sebatu District, Gianyar Regency, Bali. This case study review aims to establish the diagnosis of death in a 45-day-old female pig using anamnesis, epidemiological history, clinical symptoms, anatomical pathology examination, histopathology, and laboratory tests. The observed symptoms in the case pig included anorexia, weakness, and diarrhea. Necropsy results showed hemorrhaging in almost all organs of the case pig. Histopathological examination revealed lesions in the intestine, suggesting a Transmissible Gastroenteritis Virus (TGEV) infection, specifically intestinal villous atrophy. Bacteriological examination successfully isolated Escherichia coli bacteria in the lungs, liver, and heart. Fecal examination in the parasitology laboratory showed no evidence of worm eggs or protozoan infections. Based on a series of tests conducted, it can be concluded that the case pig is suspected to have been infected with TGEV and E. coli. Further testing is needed to investigate the co-infection of Transmissible Gastroenteritis Virus and E. coli in pigs to determine the appropriate treatment for severe diarrhea in pigs.
HETEROPHIL-LYMPHOCYTE RATIO IN LAYING HENS VACCINATED WITH NEWCASTLE DISEASE-INFECTIOUS BRONCHITIS Savira Hadi Putri; Anak Agung Sagung Kendran; Gusti Ayu Yuniati Kencana; I Made Damriyasa; Ida Bagus Kade Suardana; I Nyoman Sulabda
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p29

Abstract

Vaccination plays a role in the formation of specific immunity against certain antigens. Stressful conditions in animals can potentially influence post-vaccination immune responses. To be able to monitor this condition, it can be seen using stress indicators in poultry, namely the heterophil-lymphocyte ratio (H/L) value. This research was conducted to determine the value of the heterophil-lymphocyte ratio (H/L) in laying hens vaccinated with ND-IB. The samples used were 10 chicken blood samples taken from 40 chickens randomly during the pre-vaccination period; one week, two weeks, and three weeks post-vaccination. The research was preceded by calculating total leukocytes and leukocyte differential, followed by calculating the heterophil-lymphocyte (H/L) ratio value from the results obtained, then analyzed using the SPSS program. The results of this research show that livestock experienced mild stress in the first week post-vaccination which is thought to have occurred due to the handling carried out. A significant increase in the heterophil-lymphocyte ratio (H/L) value occurred in the second week post-vaccination, this occurred due to the influence of environmental factors in the form of bacterial infections. In the third week post-vaccination, livestock no longer experience stress. Further research needs to be carried out using negative controls to determine the exact cause of the increase in the heterophil-lymphocyte (H/L) ratio.
COMPLICATIONS OF CANINE PARVOVIRUS INFECTION WITH STREPTOCOCCUS CANIS IN A FREE-ROAMING MIXED BREED DOG: A CASE STUDY WITH ANATOMICAL PATHOLOGY AND HISTOPATHOLOGICAL Ashley; Gusti Ayu Yuniati Kencana; I Nengah Kerta Besung; I Made Dwinata; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p04

Abstract

Canine Parvovirus (CPV) is a highly contagious infectious agent in dogs, with high morbidity and significant mortality rates, especially in unvaccinated animals. This study reports a case of CPV infection exacerbated by secondary bacterial pneumonia caused by Streptococcus sp. in a 10-month-old male mixed-breed dog in Denpasar, Bali. The dog exhibited clinical symptoms for four days, including bloody diarrhea, dehydration, anorexia, coughing, dyspnea, vomiting, and nasal discharge, before succumbing on the fifth day. Diagnosis was conducted through histopathological examination, Polymerase Chain Reaction (PCR), and bacterial culture. PCR results confirmed the presence of CPV with a 900 bp fragment, while bacteriological analysis identified Streptococcus sp. in lung tissue with hemolytic activity. Macroscopic analysis revealed hemorrhagic pneumonia, edematous myocarditis, and hemorrhagic enteritis, while histopathology showed inflammatory cell infiltration and tissue necrosis. Streptococcus infection worsened the dog's condition by causing severe lung inflammation, impairing respiratory function, and increasing the risk of death. The combination of CPV and secondary infection highlights the importance of vaccination in preventing CPV and the need for early detection through PCR and bacterial culture to improve prognosis and treatment effectiveness. This study emphasizes that rapid diagnosis and appropriate supportive therapy can help reduce disease severity and lower mortality rates in infected dogs.