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All Journal Buletin Veteriner Udayana
Anak Agung Sagung Kendran
Laboratorium Patologi Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
Immunology & microbiology Public Health Veterinary

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HETEROPHIL-LYMPHOCYTE RATIO IN LAYING HENS VACCINATED WITH NEWCASTLE DISEASE-INFECTIOUS BRONCHITIS Savira Hadi Putri; Anak Agung Sagung Kendran; Gusti Ayu Yuniati Kencana; I Made Damriyasa; Ida Bagus Kade Suardana; I Nyoman Sulabda
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p29

Abstract

Vaccination plays a role in the formation of specific immunity against certain antigens. Stressful conditions in animals can potentially influence post-vaccination immune responses. To be able to monitor this condition, it can be seen using stress indicators in poultry, namely the heterophil-lymphocyte ratio (H/L) value. This research was conducted to determine the value of the heterophil-lymphocyte ratio (H/L) in laying hens vaccinated with ND-IB. The samples used were 10 chicken blood samples taken from 40 chickens randomly during the pre-vaccination period; one week, two weeks, and three weeks post-vaccination. The research was preceded by calculating total leukocytes and leukocyte differential, followed by calculating the heterophil-lymphocyte (H/L) ratio value from the results obtained, then analyzed using the SPSS program. The results of this research show that livestock experienced mild stress in the first week post-vaccination which is thought to have occurred due to the handling carried out. A significant increase in the heterophil-lymphocyte ratio (H/L) value occurred in the second week post-vaccination, this occurred due to the influence of environmental factors in the form of bacterial infections. In the third week post-vaccination, livestock no longer experience stress. Further research needs to be carried out using negative controls to determine the exact cause of the increase in the heterophil-lymphocyte (H/L) ratio.
HEMOGLOBIN VALUE OF ETAWA CROSSBREED SUFFERING FROM SUBCLINICAL MASTITIS IN RAKA ETAWA FARM, SINGAPADU KALER GIANYAR BALI Christine Ratu Azalia Kero; Anak Agung Sagung Kendran; Romy Muhammad Dary Mufa; Siswanto; I Made Sukada; Putu Devi Jayanti
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p33

Abstract

One of the health problems that occurs in Etawa crossbreed (PE) goat farms as dairy goats is subclinical mastitis. Subclinical mastitis is potential mastitis that does not show clinical symptoms in the udder. In an effort to support the health of PE goats as dairy livestock, it is necessary to carry out routine health checks, one of which is blood tests such as hemoglobin tests. This study aims to determine the hemoglobin value of Etawa crossbreed goats (PE) suffering from subclinical mastitis. Blood samples were taken after the CMT test on goats that were positive for subclinical mastitis. After that, a blood sample was taken from the jugular vein area using needle blood colection with the help of vacuntainer holder and put in coolbox. The blood samples that have been taken are then examined using a Hematology Analyzer RT 7600. The data obtained is then analyzed descriptively regarding hemoglobin values. The research results showed that hemoglobin in PE goats showed values ​​within the normal range, namely 8.46 ± 0.85 g/dL with a range of 7.4 – 9.6 g/dL. To support better results, farmers need to maintain the health of their livestock by routinely carrying out livestock checks, including blood tests.  
MACROSCOPIC ANALYSIS OF INCISION WOUND HEALING IN WHITE RATS TREATED WITH PLATELET-RICH PLASMA DERIVED FROM PORCINE BLOOD Made Sandy Widhi Raharja; I Wayan Wirata; Anak Agung Sagung Kendran; I Gusti Ngurah Sudisma; I Wayan Gorda; I Ketut Berata
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p10

Abstract

Platelet-rich plasma (PRP) has been identified as a potential therapeutic agent to mitigate factors that impede healing. PRP, which is enriched with growth factors, plays a critical role in accelerating tissue regeneration across various stages of wound repair. This study aimed to investigate the effects of PRP derived from porcine blood on the healing of incisional wounds in white rats (Rattus norvegicus). Macroscopic parameters, including wound length, wound color, moisture levels, and crust formation, were evaluated. The study employed an experimental design, utilizing a split-time completely randomized design (CRD) with statistical analysis. The results demonstrated significant improvements in wound closure, as evidenced by reduced wound length, accelerated changes in wound color, maintained wound moisture leading to faster drying, and enhanced crust formation, all of which contributed to a reduction in wound size (P < 0.05). These findings indicate that PRP derived from porcine blood significantly accelerates the healing process of incisional wounds in white rats, as reflected by macroscopic healing indicators. Further research is recommended to determine the optimal shelf life of PRP to ensure its efficacy and stability during prolonged storage, thereby supporting its sustainable clinical application.
Co-Authors Christine Ratu Azalia Kero Gusti Ayu Yuniati Kencana I Gusti Ngurah Sudisma I Ketut Berata I Made Damriyasa I Made Sukada I Nyoman Sulabda I Wayan Gorda I Wayan Wirata Ida Bagus Kade Suardana Made Sandy Widhi Raharja Putu Devi Jayanti Romy Muhammad Dary Mufa Savira Hadi Putri Siswanto