I Gusti Ketut Suarjana
Laboratorium Bakteriologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia 80234

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RESISTANCE PATTERN OF ANTIBIOTICS ON KLEBSIELLA SPP. FROM FECES OF BALI CATTLE SOLD AT BERINGKIT LIVESTOCK MARKET Ivan Christsatria Sophian Merling; Hapsari Mahatmi; I Gusti Ketut Suarjana; I Gede Putra Sanjaya
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p30

Abstract

Beringkit Animal Market is one of the largest animal markets in Bali. Which is a place for livestock transactions, especially bali cattle calves, which are usually used as breeds. Bali cattle calves need digestive tract microbes such as bacteria to carry out the digestives process. Klebsiella spp. is a type of coliform bacteria that plays a significant role in the digestive system of bali cattle. This study aimed to determine the presence of Klebsiella spp. and antibiotic resistance patterns in bali cattle marketed at Beringkit Livestock Market. The samples used were rectal swabs from 50 bali cattle. First, swab samples were isolated and identified, starting with isolation on Eosin Methlyne Blue Agar (EMBA), followed by colony characterization and biochemical tests such as Indole Metil Voges Proskauer Citrate (IMViC). Then, the identified Klebsiella spp. colonies were tested for antibiotic resistance patterns using 8 antibiotic discs, including bacitracin, ampicillin, kanamycin, streptomycin, doxycycline, erythromycin, trimethoprim-sulfamethoxazole, and chloramphenicol. The method of examination used the Kirby Bauer antibiotic disc diffusion method. The prevalence of Klebsiella spp. was found to be 6% among 50 cattle at Beringkit Livestock Market. The resistance pattern showed that sample no. 12 was resistant to bacitracin, ampicillin, streptomycin, doxycycline, and erythromycin; intermediate to kanamycin; and sensitive to sulfamethoxazole-trimethoprim and chloramphenicol. Sample no. 33 was resistant to bacitracin, ampicillin, streptomycin, doxycycline, and erythromycin; intermediate to kanamycin; and sensitive to sulfamethoxazole-trimethoprim and chloramphenicol. Sample no. 44 was resistant to bacitracin, ampicillin, streptomycin, erythromycin, and chloramphenicol; intermediate to kanamycin, sulfamethoxazole-trimethoprim; and sensitive to doxycyclin. Sulfamethoxazole-trimethoprim and kanamycin are still suitable for use due to a sensitivity rate of 66% in three Klebsiella spp. samples. However, antibiotics such as bacitrasin, ampicillin, streptomycin, doxycycline, erythromycin, and chloramphenicol should be discontinued due to high resistance rates, and further research is needed to find effective and suitable antibiotics for managing Klebsiella spp. infections.
CANINE PARVOVIRUS ENTERITIS INFECTION IN LOCAL DOGS IN SAMPLANGAN VILLAGE, GIANYAR REGENCY, BALI Fauzia Hadista Anjani; Ida Bagus Kade Suardana; I Gusti Ketut Suarjana; Ida Bagus Oka Winaya; Ida Ayu Pasti Apsari
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2025 Vol. 17 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i05.p01

Abstract

A common disease that poses a threat to dogs is Canine Parvovirus (CPV). Puppies tend to be more susceptible to CPV infection due to a period called the window of susceptibility. The purpose of this case study is to determine clinical changes that include anatomical pathology, histopathology, and laboratory examinations to establish a definitive diagnosis of the case animal. The animal used in the case study was a four-month-old female local puppy from Gianyar, Bali. The case dog was found freshly dead, and had been sick for 4 days. The dog showed symptoms of weakness, anorexia, and vomiting, bloody diarrhea. Polymerase Chain Reaction (PCR) test results were positive for Canine Parvovirus infection. Anatomical pathology changes found were congestion of the brain, hemorrhage of the trachea, swelling of the heart, hemorrhage of the liver, hemorrhage of the patu-peru, hyperemia of the renal medulla, blackening and swelling of the spleen, hemorrhage of the intestines, and bleeding of the stomach. Histopathological changes observed were tracheitis, necrotizing bronchopneumonia, myocarditis, hemorrhagic glomerulo-nephritis, speinitis, gastritis, and necrotizing enteritis. Based on clinical symptoms, anatomical and histopathological pathology observations, and Polymerase Chain Reaction (PCR) results, it can be concluded that the case dog was infected with Canine Parvovirus type enteritis. It is necessary to vaccinate dogs and pay attention to environmental cleanliness to minimize the transmission of Canine Parvovirus infection.