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All Journal Buletin Veteriner Udayana
Ni Luh Eka Setiasih
Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia 80234
Immunology & microbiology Public Health Veterinary

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COMPARISON OF ANTIBODY TITERS IN BROILERS GIVEN GREEN MENIRAN EXTRACT AT DIFFERENT DOSES FOR TWO WEEKS BEFORE NEWCASTLE DISEASE BOOSTER VACCINATION Dewa Kade Agung Hendra Putra Dinata; Ida Bagus Kade Suardana Suardana; I Ketut Suada; Ni Luh Eka Setiasih; I Made Kardena; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p26

Abstract

Newcastle Disease (ND) is a viral disease that affects poultry, including broilers. One of the preventive measures is vaccination; however, its effectiveness remains suboptimal, necessitating an alternative approach such as green meniran (Phyllanthus niruri Linn.) extract to enhance antibody titers. This study aims to determine the differences in antibody titers in broilers administered green meniran extract at different doses for two weeks before the ND booster vaccination. The study involved 30 broilers aged 1 to 32 days. The experimental design followed a Completely Randomized Design with a hierarchical pattern consisting of three treatment groups. Serum samples were collected one day before the ND booster vaccination (17 days old), oneweek post-booster vaccination (25 days old), and two weeks post-booster vaccination (32 days old). The serum samples were tested using the Hemagglutination Inhibition (HI) assay. Data were analyzed using Analysis of Variance (ANOVA) and regression analysis. ANOVA results showed that the treatment group (P2) receiving green meniran extract at a dose of 6 mg/kg BW/day exhibited a significant increase (P<0.05) compared to the treatment group (P1) receiving 4 mg/kg BW/day. Regression analysis indicated that each group experienced an increase in antibody titers up to the fourth week post-booster vaccination. The findings suggest that different sampling times significantly (P<0.05) influenced the antibody titers formed. In conclusion, green meniran extract as a natural immunostimulator can enhance the immune response in broiler chickens. The combination of green meniran extract at doses of 4 mg/kg BW/day and 6 mg/kg BW/day for two weeks with ND booster vaccination proved to be more effective in increasing ND antibody titers in broilers than vaccination alone.
HISTOLOGICAL FEATURES OF LYMPHOCYTES, MONOCYTES AND HEMOGLOBIN ONCENTRATIONS IN DOGS WITH MILD AND SEVERE DERMATITIS Ni Putu Dewi Rahmawati; Ni Ketut Suwiti; Sri Kayati Widyastuti; Nyoman Sadra Dharmawan; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p30

Abstract

Dogs are susceptible to various dermatological disorders, among which dermatitis is commonly observed. This condition may be caused by fungal infections, ectoparasites, bacterial agents, or metabolic disorders. Dermatitis presents with clinical manifestations ranging from mild to severe, often characterized by widespread skin lesions. The associated inflammatory response may induce alterations in the immune system, particularly affecting agranulocytic leukocytes namely lymphocytes and monocytes or leading to cellular necrosis. Severe dermatitis is frequently accompanied by secondary infections, which may elevate the risk of inflammation-induced anemia and result in changes to hemoglobin (HGB) levels. This study aims to investigate the histological features of necrotic lymphocytes and monocytes, as well as hemoglobin (HGB) levels, in dogs affected by mild and severe dermatitis. Histological examination revealed that necrotic lymphocytes and monocytes exhibited signs of pyknosis, karyorrhexis, and karyolysis. The number of necrotic lymphocytes in dogs with mild dermatitis (10±4.64) was slightly higher than in those with severe dermatitis (8.65±3.38), although the difference was not statistically significant (P˃0.05). In contrast, the number of necrotic monocytes in mild dermatitis cases (19.3±20.8) was significantly greater than in severe cases (3.35±11.62) (P˂0.05). Hemoglobin levels in dogs with mild dermatitis (10.75±4.23) were marginally lower than those in severe cases (11.23±2.9), with no statistically significant difference (P˃0.05).
THE THE EFFECT OF GREEN MENIRAN EXTRACT ON AVIAN INFLUENZA ANTIBODY TITER IN BROILERS TWO WEEKS BEFORE VACCINATION Dewandaru Evan Wintyassatya; Ida Bagus Kade Suardana; Romy Muhammad Dary Mufa; I Made Sukada; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p32

Abstract

Avian Influenza (AI) is a viral disease that poses a serious threat to poultry farming, particularly in broiler production. The rapid and massive spread of AI in poultry flocks can cause substantial economic losses for farmers. Vaccination is one of the primary strategies for AI prevention; however, the currently available AI vaccines are killed/inactivated types, which tend to induce antibody titers slowly. This limitation, coupled with the relatively short rearing period of broilers, reduces vaccine effectiveness in broiler chickens. This study aimed to evaluate the effect of green meniran (Phyllanthus niruri Linn.) extract on enhancing antibody titers in broilers when administered for two weeks prior to AI vaccination. A total of 30 broiler chickens aged from day 1 to 32 days were used as experimental subjects. The study employed a Completely Randomized Design (CRD) with three groups: negative control (K−), positive control (K+), and treatment group (P). Data were analyzed using Analysis of Variance (ANOVA) and Regression Analysis via SPSS software. The results demonstrated a comparative increase in average antibody titers in the treatment group receiving Phyllanthus niruri extract compared to the control groups. It was concluded that supplementation with Phyllanthus niruri extract effectively enhanced antibody titers in broilers vaccinated with the inactivated AI La Sota vaccine, with peak antibody responses observed 1–2 weeks post-vaccination. The administration of green Phyllanthus niruri extract has been proven to stimulate antibody titers in broilers by accelerating the development of protective antibody levels against the AI virus. The timing of sample collection was shown to significantly affect antibody titers, with the highest levels observed during the first to second week post-vaccination. Future research on the administration of Phyllanthus niruri extract to improve antibody titers against Avian Influenza (AI) in broilers is expected to extend the duration of supplementation until the harvesting period and to include higher dosage variations in order to achieve more optimal and protective results.
Co-Authors Dewa Kade Agung Hendra Putra Dinata Dewandaru Evan Wintyassatya I Ketut Suada I Made Kardena I Made Sukada Ida Bagus Kade Suardana Ida Bagus Kade Suardana Suardana Ni Ketut Suwiti Ni Putu Dewi Rahmawati Nyoman Sadra Dharmawan Romy Muhammad Dary Mufa Sri Kayati Widyastuti Tjokorda Sari Nindhia