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Effects of Naphthaleneacetic Acid and Kinetin on Chrysanthemum Plantlets in Vitro Saipul Sihotang; Magdalena Saragih; Swati Sembiring; Riduan Sembiring; Dini Puspita Yanty; Denny Akbar Tanjung; Elli Afrida
Journal of Applied Agricultural Science and Technology Vol. 9 No. 4 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i4.315

Abstract

Naphthaleneacetic acid (NAA) and kinetin play a crucial role in plant growth but must be used in correct proportions in order to produce the best outcomes. An optimal combination of both can enhance shoot, leaf, and root growth, whereas inappropriate doses may otherwise inhibit growth due to hormonal imbalance. This study examined the effects of the interaction between NAA and kinetin on the growth of chrysanthemum plants at eight weeks of age. The research was carried out using the Completely Randomized Design (CRD) in triplicate. The medium used was MS medium, supplemented with NAA at concentrations of 0–1.5 mg/L and kinetin at concentrations of 0–3 mg/L. The results indicate that NAA and kinetin positively influenced plant growth, but only when the optimal concentrations were applied. The best combination of NAA at 1.0 mg/L (N2) and kinetin at 3.0 mg/L (K3) produced the best results in terms of height (153 mm), number of leaves (31 leaves), number of roots (26 roots), and fresh weight (5.85 g). Increasing kinetin concentrations (K0-3) generally promoted plant growth, whereas excessive NAA concentrations (> 1.0 mg/L, N3) produced the opposite effect, likely due to toxicity or hormonal imbalance. The interaction between kinetin and NAA exhibited a synergistic effect up to a certain threshold, while excessive NAA application negatively impacted on plant development.
Growth and Yield Response of Shallot (Allium ascalonicum L.) to Various Concentrations of Eco-Enzyme Fertilizer: English Swati Sembiring; Lita Nasution; Dedi Holden Simbolon; Magdalena Saragih; Donatus Dahang; Rajiv Ginting; Juliana Simbolon; Yolanda Febrima Tarigan; Rifa Diah Valahsya Sembiring; Indra Prabowo Pandiangan; Eristo Syahmana Tarigan; Sri Rejeki Silaban
Asian Multidisciplinary Research Journal of Economy and Learning Vol. 3 No. 5 (2026): Integrative Health and Sustainable Agriculture for Community Well-Being
Publisher : Argael Publisher, an imprint of CV. ARGA FARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70471/jh9ck595

Abstract

Shallot (Allium ascalonicum L.) is a horticultural commodity with high economic value, but its productivity is often constrained by declining soil fertility associated with prolonged and intensive inorganic fertilization. This study evaluated the growth and yield response of shallot to various eco-enzyme fertilizer dosages and identified the most effective dosage under polybag-based open-field experimental conditions. The experiment was arranged in a non-factorial Randomized Complete Block Design (RCBD) with five treatments and five replications: E0 (without eco-enzyme), E1 (100 mL plot⁻¹), E2 (200 mL plot⁻¹), E3 (300 mL plot⁻¹), and E4 (400 mL plot⁻¹). Each plot referred to one polybag experimental unit. The observed variables were plant height, number of leaves, number of tillers, fresh bulb weight, and dry bulb weight. The results showed that eco-enzyme dosage affected several vegetative variables at specific observation times, but the response was not uniform across all growth stages. E2 produced the highest plant height at 14, 28, and 42 days after planting (DAP), whereas E3 produced the highest leaf number at 28 DAP. Yield formation was more favorable under the lowest eco-enzyme dosage. Treatment E1 produced the highest fresh bulb weight (167.2 g plot⁻¹) and dry bulb weight (131.2 g plot⁻¹), corresponding to approximately 5.03% and 19.06% increases over the control, respectively. These findings indicate that 100 mL plot⁻¹ was the most efficient eco-enzyme dosage for improving bulb yield of Bima Brebes shallot under the conditions of this experiment.