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Kultur Slide Sebagai Metode Mikroskopik Tidak Langsung untuk Identifikasi Jamur Kapang Conny Riana Tjampakasari; Riani Agustini; Ichwan Baihaki; Shoffiana Noor; Arleni Bustami
Jurnal Sehat Indonesia (JUSINDO) Vol. 6 No. 01 (2024): Jurnal Sehat Indonesia (JUSINDO)
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jsi.v6i01.75

Teknik kultur slide merupakan pengamatan mikroskopis tidak langsung yang digumnakan untuk mengidentifikasi isolat jamur berdasarkan hifa yang dibentuk. Dengan metode ini pemeriksaan secara rinci jamur berfilamen tetap menjadi salah satu prosedur andalan untuk identifikasi morfologi jamur di laboratorium mikologi diagnostik di seluruh dunia. Identifikasi yang akurat berdasarkan visualisasi detail mikroskopis seperti bentuk, susunan konidia yang halus dan perlekatan konidia dengan sel konidia sangat penting dan memiliki pengaruh pada manajemen klinis mikosis. Pada pemeriksaan ini telah berhasil dilakukan identifikasi Aspergillus mengunakan teknik kultur slide. Diperoleh gambaran struktur hifa, konidiofora, vesikel, sterigmata dan konidia diatasnya. Metode ini merupakan teknik identifikasi yang paling umum untuk identifikasi jamur kapang patogen. Teknik ini relatif lebih tahan lama untuk menyimpan gambaran morfologi dibandingkan metode selotip. Walaupun demikian, perlu dilakukan modifikasi metode untuk meningkatkan kegunaannya dan mempersingkat waktu untuk mengidentifikasi.

Neuroprotective Effect of Red Betel (Piper crocatum) Extract on Brain Monosodium Gluatame (MSG)-Induced Rat (Rattus norvegicus) Baihaki, Ichwan; Mutmainnah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2373

Indonesian people prefer to eat fast food which is generally rich in flavorings. The most common ingredient found in fast food is MSG (monosodium glutamate). MSG consumption that exceeds the limit will produce free glutamate which is excitotoxic in various organs, one of which is the neurological system. Red betel has a fairly high antioxidant content which can inhibit excitotoxicity by inhibiting the occurrence of free radicals. The aim of this research was to determine the neuroprotective effect of red betel extract (Piper crocatum) on the brains of rats (Rattus norvegicus) given monosodium glutamate (MSG). This research is a laboratory experimental study with a completely randomized design method using 5 treatment levels, namely negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3). The NC treatment level was given 2 mL of distilled water. The PC treatment level was given 7gr/KgBB MSG. Levels T1, T2, T3 were each given MSG 7gr/KgBW in the morning, then after 8 hours, level P1 was given 200mg/KgBW of red betel extract, P2 was given 400mg/KgBW of red betel extract, and P3 was given 600mg/KgBW of red betel extract. Examination of the number of neurons in the dentate gyrus of the hippocampus using the ImageJ application and examination of calcium ion levels using the RAPIDLab 348X tool. Data analysis used the One Way Anova test with the SPSS 24 program. The results showed that there was a significant difference (p<0.05) between PC and NC. The results of the calcium ion parameters showed that only the 600mg dose treatment group functioned as a protector in the occurrence of hypocalcemia, while the histopathology parameters showed that the 400mg and 600mg dose treatment groups functioned significantly as a neuroprotector.

Neuroprotective Effect of Red Betel (Piper crocatum) Extract on Brain Monosodium Gluatame (MSG)-Induced Rat (Rattus norvegicus) Baihaki, Ichwan; Mutmainnah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2373

Indonesian people prefer to eat fast food which is generally rich in flavorings. The most common ingredient found in fast food is MSG (monosodium glutamate). MSG consumption that exceeds the limit will produce free glutamate which is excitotoxic in various organs, one of which is the neurological system. Red betel has a fairly high antioxidant content which can inhibit excitotoxicity by inhibiting the occurrence of free radicals. The aim of this research was to determine the neuroprotective effect of red betel extract (Piper crocatum) on the brains of rats (Rattus norvegicus) given monosodium glutamate (MSG). This research is a laboratory experimental study with a completely randomized design method using 5 treatment levels, namely negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3). The NC treatment level was given 2 mL of distilled water. The PC treatment level was given 7gr/KgBB MSG. Levels T1, T2, T3 were each given MSG 7gr/KgBW in the morning, then after 8 hours, level P1 was given 200mg/KgBW of red betel extract, P2 was given 400mg/KgBW of red betel extract, and P3 was given 600mg/KgBW of red betel extract. Examination of the number of neurons in the dentate gyrus of the hippocampus using the ImageJ application and examination of calcium ion levels using the RAPIDLab 348X tool. Data analysis used the One Way Anova test with the SPSS 24 program. The results showed that there was a significant difference (p<0.05) between PC and NC. The results of the calcium ion parameters showed that only the 600mg dose treatment group functioned as a protector in the occurrence of hypocalcemia, while the histopathology parameters showed that the 400mg and 600mg dose treatment groups functioned significantly as a neuroprotector.

Effectiveness of Mesenchymal Stem Cells and Their Derivatives in Modulating Oxidative Stress in Neurodegenerative Diseases: A Structured Narrative Review Ghanny, Niken Rahmah; Pradnyani, Putu Erma; Salwa, Assyafiya; Rahajeng, Herera; Radhina, Afifa; Sari, Bunga Anggreini; Baihaki, Ichwan
Indonesian Journal of Life Sciences 2026: IJLS Vol 08 No.01
Publisher : Universitas Bio Scientia Internasional Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54250/ijls.v8i01.297

Background: Oxidative stress plays a critical role in the development and progression of neurodegenerative diseases such as Parkinson’s disease (PD), Alzheimer’s disease (AD), and amyotrophic lateral sclerosis (ALS). Mesenchymal stem cells (MSCs) and their derivatives have emerged as promising therapeutic strategies due to their antioxidant, anti-inflammatory, and neuroprotective properties. Objective: This review evaluated the effectiveness of MSC-based interventions in modulating oxidative stress in neurodegenerative disease models. Methods: A structured narrative review search was conducted following PRISMA 2020 guidelines using PubMed and Scopus databases for studies published between 2020 and 2025, with the last search in December 2025. Results: 33 studies met the inclusion criteria, primarily involving Parkinson’s and Alzheimer’s disease models. Overall, MSC-based therapies reduced oxidative stress markers, enhanced antioxidant defenses, activated the Nrf2/HO-1 pathway, improved mitochondrial function, and reduced neuroinflammation in experimental neurodegenerative disease models.

Pengaruh Pemberian Virgin Coconut Oil Terhadap Kadar TNF- dan Jumlah Leukosit Pada Tikus Putih Jantan (Rattus norvegicus) Model Hiperkolesterolemia Mutmainnah; Ichwan Baihaki
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 2 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i2.3130

Hypercholesterolemia contributes to oxidative stress, which may cause dysregulation of the proinflammatory response as indicated by increased levels of TNF-α and leukocyte count, both of which are risk factors for metabolic syndrome. Exogenous antioxidants such as tocopherol are required to interrupt the chain reactions of free radicals, while lauric acid functions as an anti-inflammatory agent that enhances immunity. These compounds are contained in virgin coconut oil (VCO). Aimed to determine the effect of VCO administration on the reduction of total cholesterol levels, TNF-α concentrations, and leukocyte counts. This experimental study employed a post-test only control group design. Twenty-four male Wistar rats were randomly divided into four groups. Group K0 received neither a high-cholesterol diet nor VCO. Group K1 received a high-cholesterol diet without VCO. Groups P1 and P2 received a high-cholesterol diet along with VCO at doses of 0.9 mL/200 g BW/day and 0.45 mL/200 g BW/day, respectively. On day 21, blood samples were collected to measure total cholesterol, TNF-α, and leukocyte count at the PSPG UGM Laboratory. The One-way ANOVA showed significant differences in TNF-α and leukocyte counts among groups (p=0.000). Post hoc Tamhane’s and Tukey tests demonstrated that TNF-α and leukocyte levels in groups K0, P1, and P2 differed significantly from those in K1 (p<0.05). Administration of virgin coconut oil effectively reduced TNF-α, and leukocyte count levels. Keywords: Leukocyte counts, TNF-α, VCO

Kesintasan Hidup Satu Tahun Pasien Sirosis Hati yang Dirawat di Rumah Sakit Cipto Mangunkusumo pada Periode COVID-19 Aprilicia, Gita; Baihaki, Ichwan; Banjarnahor, Riri Oktavani; Kalista, Kemal Fariz; Gani, Rino Alvani
Jurnal Penyakit Dalam Indonesia
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Introduction. Liver cirrhosis represents the terminal stage of chronic liver disease and is characterized by a markedly reduced survival rate. Understanding the characteristics and laboratory parameters of patients with liver cirrhosis can provide valuable insights into their prognosis and potential interventions. This study was conducted during the COVID-19 pandemic period, which may have influenced patient outcomes due to potential impact of COVID-19 infection on patients with chronic liver disease. This study aimed to evaluate the one-year survival rate of hospitalized liver cirrhosis patients at Cipto Mangunkusumo Hospital during the COVID-19 and to identify clinical and laboratory factors associated with patient survival. Methods. This retrospective study analyzed data from 73 in-hospital liver cirrhosis patients during January to December 2020. Demographic characteristics, comorbidities, diagnosis upon admission, and laboratory parameters were collected. Patients were categorized into two groups: those who survived and those who died within one year of hospitalization. Survival analysis was performed using the Kaplan–Meier method to estimate 1 year survival probability of cirrhosis patients. Results. The analysis revealed significant differences in several characteristics and laboratory parameters between the survival and death groups during COVID-19 period. The etiology of liver cirrhosis was predominantly related to HBV (41.1%) and HCV (23.3%), which constituted the most common causes. Diagnosis upon admission showed a significant association with survival, with bleeding esophageal varices (53.4%) being the most prevalent. Laboratory parameters such as SGOT, SGPT, albumin, bilirubin, prothrombin time, INR, and Child Pugh score demonstrated significant differences between the two groups. The 1-year survival rate for the Child Pugh class A, B and C was approximately 100%, 61.2% (CI 95% 42.2 - 80.2%) and 0%, respectively. The overall 1-year survival rate of in-hospital liver cirrhosis patients was 52.6% (CI 95% 38.7 - 66.5%). Conclusions. This study found the overall survival of in-hospital liver cirrhosis patients during COVID-19 period was low. It’s essential to develop a comprehensive and personalized treatment plan for optimal patient outcomes.

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