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Kultur Slide Sebagai Metode Mikroskopik Tidak Langsung untuk Identifikasi Jamur Kapang Conny Riana Tjampakasari; Riani Agustini; Ichwan Baihaki; Shoffiana Noor; Arleni Bustami
Jurnal Sehat Indonesia (JUSINDO) Vol. 6 No. 01 (2024): Jurnal Sehat Indonesia (JUSINDO)
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jsi.v6i01.75

Teknik kultur slide merupakan pengamatan mikroskopis tidak langsung yang digumnakan untuk mengidentifikasi isolat jamur berdasarkan hifa yang dibentuk. Dengan metode ini pemeriksaan secara rinci jamur berfilamen tetap menjadi salah satu prosedur andalan untuk identifikasi morfologi jamur di laboratorium mikologi diagnostik di seluruh dunia. Identifikasi yang akurat berdasarkan visualisasi detail mikroskopis seperti bentuk, susunan konidia yang halus dan perlekatan konidia dengan sel konidia sangat penting dan memiliki pengaruh pada manajemen klinis mikosis. Pada pemeriksaan ini telah berhasil dilakukan identifikasi Aspergillus mengunakan teknik kultur slide. Diperoleh gambaran struktur hifa, konidiofora, vesikel, sterigmata dan konidia diatasnya. Metode ini merupakan teknik identifikasi yang paling umum untuk identifikasi jamur kapang patogen. Teknik ini relatif lebih tahan lama untuk menyimpan gambaran morfologi dibandingkan metode selotip. Walaupun demikian, perlu dilakukan modifikasi metode untuk meningkatkan kegunaannya dan mempersingkat waktu untuk mengidentifikasi.

Neuroprotective Effect of Red Betel (Piper crocatum) Extract on Brain Monosodium Gluatame (MSG)-Induced Rat (Rattus norvegicus) Baihaki, Ichwan; Mutmainnah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2373

Indonesian people prefer to eat fast food which is generally rich in flavorings. The most common ingredient found in fast food is MSG (monosodium glutamate). MSG consumption that exceeds the limit will produce free glutamate which is excitotoxic in various organs, one of which is the neurological system. Red betel has a fairly high antioxidant content which can inhibit excitotoxicity by inhibiting the occurrence of free radicals. The aim of this research was to determine the neuroprotective effect of red betel extract (Piper crocatum) on the brains of rats (Rattus norvegicus) given monosodium glutamate (MSG). This research is a laboratory experimental study with a completely randomized design method using 5 treatment levels, namely negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3). The NC treatment level was given 2 mL of distilled water. The PC treatment level was given 7gr/KgBB MSG. Levels T1, T2, T3 were each given MSG 7gr/KgBW in the morning, then after 8 hours, level P1 was given 200mg/KgBW of red betel extract, P2 was given 400mg/KgBW of red betel extract, and P3 was given 600mg/KgBW of red betel extract. Examination of the number of neurons in the dentate gyrus of the hippocampus using the ImageJ application and examination of calcium ion levels using the RAPIDLab 348X tool. Data analysis used the One Way Anova test with the SPSS 24 program. The results showed that there was a significant difference (p<0.05) between PC and NC. The results of the calcium ion parameters showed that only the 600mg dose treatment group functioned as a protector in the occurrence of hypocalcemia, while the histopathology parameters showed that the 400mg and 600mg dose treatment groups functioned significantly as a neuroprotector.

Neuroprotective Effect of Red Betel (Piper crocatum) Extract on Brain Monosodium Gluatame (MSG)-Induced Rat (Rattus norvegicus) Baihaki, Ichwan; Mutmainnah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2373

Indonesian people prefer to eat fast food which is generally rich in flavorings. The most common ingredient found in fast food is MSG (monosodium glutamate). MSG consumption that exceeds the limit will produce free glutamate which is excitotoxic in various organs, one of which is the neurological system. Red betel has a fairly high antioxidant content which can inhibit excitotoxicity by inhibiting the occurrence of free radicals. The aim of this research was to determine the neuroprotective effect of red betel extract (Piper crocatum) on the brains of rats (Rattus norvegicus) given monosodium glutamate (MSG). This research is a laboratory experimental study with a completely randomized design method using 5 treatment levels, namely negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3). The NC treatment level was given 2 mL of distilled water. The PC treatment level was given 7gr/KgBB MSG. Levels T1, T2, T3 were each given MSG 7gr/KgBW in the morning, then after 8 hours, level P1 was given 200mg/KgBW of red betel extract, P2 was given 400mg/KgBW of red betel extract, and P3 was given 600mg/KgBW of red betel extract. Examination of the number of neurons in the dentate gyrus of the hippocampus using the ImageJ application and examination of calcium ion levels using the RAPIDLab 348X tool. Data analysis used the One Way Anova test with the SPSS 24 program. The results showed that there was a significant difference (p<0.05) between PC and NC. The results of the calcium ion parameters showed that only the 600mg dose treatment group functioned as a protector in the occurrence of hypocalcemia, while the histopathology parameters showed that the 400mg and 600mg dose treatment groups functioned significantly as a neuroprotector.

Pengaruh Pemberian Virgin Coconut Oil Terhadap Kadar TNF- dan Jumlah Leukosit Pada Tikus Putih Jantan (Rattus norvegicus) Model Hiperkolesterolemia Mutmainnah; Ichwan Baihaki
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 2 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i2.3130

Hypercholesterolemia contributes to oxidative stress, which may cause dysregulation of the proinflammatory response as indicated by increased levels of TNF-α and leukocyte count, both of which are risk factors for metabolic syndrome. Exogenous antioxidants such as tocopherol are required to interrupt the chain reactions of free radicals, while lauric acid functions as an anti-inflammatory agent that enhances immunity. These compounds are contained in virgin coconut oil (VCO). Aimed to determine the effect of VCO administration on the reduction of total cholesterol levels, TNF-α concentrations, and leukocyte counts. This experimental study employed a post-test only control group design. Twenty-four male Wistar rats were randomly divided into four groups. Group K0 received neither a high-cholesterol diet nor VCO. Group K1 received a high-cholesterol diet without VCO. Groups P1 and P2 received a high-cholesterol diet along with VCO at doses of 0.9 mL/200 g BW/day and 0.45 mL/200 g BW/day, respectively. On day 21, blood samples were collected to measure total cholesterol, TNF-α, and leukocyte count at the PSPG UGM Laboratory. The One-way ANOVA showed significant differences in TNF-α and leukocyte counts among groups (p=0.000). Post hoc Tamhane’s and Tukey tests demonstrated that TNF-α and leukocyte levels in groups K0, P1, and P2 differed significantly from those in K1 (p<0.05). Administration of virgin coconut oil effectively reduced TNF-α, and leukocyte count levels. Keywords: Leukocyte counts, TNF-α, VCO

Kesintasan Hidup Satu Tahun Pasien Sirosis Hati yang Dirawat di Rumah Sakit Cipto Mangunkusumo pada Periode COVID-19 Aprilicia, Gita; Baihaki, Ichwan; Banjarnahor, Riri Oktavani; Kalista, Kemal Fariz; Gani, Rino Alvani
Jurnal Penyakit Dalam Indonesia Vol. 13, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Introduction. Liver cirrhosis represents the terminal stage of chronic liver disease and is characterized by a markedly reduced survival rate. Understanding the characteristics and laboratory parameters of patients with liver cirrhosis can provide valuable insights into their prognosis and potential interventions. This study was conducted during the COVID-19 pandemic period, which may have influenced patient outcomes due to potential impact of COVID-19 infection on patients with chronic liver disease. This study aimed to evaluate the one-year survival rate of hospitalized liver cirrhosis patients at Cipto Mangunkusumo Hospital during the COVID-19 and to identify clinical and laboratory factors associated with patient survival. Methods. This retrospective study analyzed data from 73 in-hospital liver cirrhosis patients during January to December 2020. Demographic characteristics, comorbidities, diagnosis upon admission, and laboratory parameters were collected. Patients were categorized into two groups: those who survived and those who died within one year of hospitalization. Survival analysis was performed using the Kaplan–Meier method to estimate 1 year survival probability of cirrhosis patients. Results. The analysis revealed significant differences in several characteristics and laboratory parameters between the survival and death groups during COVID-19 period. The etiology of liver cirrhosis was predominantly related to HBV (41.1%) and HCV (23.3%), which constituted the most common causes. Diagnosis upon admission showed a significant association with survival, with bleeding esophageal varices (53.4%) being the most prevalent. Laboratory parameters such as SGOT, SGPT, albumin, bilirubin, prothrombin time, INR, and Child Pugh score demonstrated significant differences between the two groups. The 1-year survival rate for the Child Pugh class A, B and C was approximately 100%, 61.2% (CI 95% 42.2 - 80.2%) and 0%, respectively. The overall 1-year survival rate of in-hospital liver cirrhosis patients was 52.6% (CI 95% 38.7 - 66.5%). Conclusions. This study found the overall survival of in-hospital liver cirrhosis patients during COVID-19 period was low. It’s essential to develop a comprehensive and personalized treatment plan for optimal patient outcomes.

Hubungan Nilai Immature Platelet Fraction (IPF) Dengan Kejadian Trombositopenia Pada Pasien Demam Berdarah Dengue Baihaki, Ichwan; Aprilicia, Gita
Borneo Journal of Medical Laboratory Technology Vol. 8 No. 2 (2026): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v8i2.12150

Demam berdarah dengue (DBD) merupakan masalah kesehatan utama di negara tropis dengan manifestasi khas trombositopenia, namun mekanisme dan respon sumsum tulang terhadap penurunan trombosit belum sepenuhnya dimanfaatkan dalam praktik klinis. Immature Platelet Fraction (IPF) menggambarkan fraksi trombosit muda di sirkulasi dan berpotensi menjadi penanda tidak langsung trombopoiesis pada trombositopenia terkait infeksi dengue. Tujuan penelitian ini enilai hubungan nilai IPF dengan kejadian trombositopenia pada pasien DBD dengan antigen NS1 positif serta membandingkan nilai IPF pasien DBD dan non DBD. Studi deskriptif analitik menggunakan data sekunder laboratorium dari 14 pasien DBD dengan trombositopenia dan 14 subjek sehat sebagai kontrol di Rumah Sakit Yarsi Jakarta, periode November 2024-Maret 2025; pemeriksaan DENV dilakukan dengan uji cepat antigen NS1 dan IgM/IgG, sedangkan IPF diukur menggunakan Sysmex XN‑1000, kemudian dianalisis dengan uji T independen dan uji korelasi Pearson. Hasil rerata IPF pasien DBD adalah 8,5% (SD 2,34) dan pasien non DBD 2,3% (SD 1,36) dengan perbedaan bermakna (p<0,05), serta 93% pasien DBD dengan trombositopenia menunjukkan IPF tinggi. Terdapat korelasi negatif yang kuat dan signifikan antara jumlah trombosit dan IPF (p<0,05; r = -0,79), yang menunjukkan bahwa penurunan trombosit berkaitan dengan peningkatan IPF. Nilai IPF meningkat secara bermakna pada pasien DBD dengan trombositopenia dan berkorelasi kuat dengan penurunan jumlah trombosit, sehingga IPF berpotensi digunakan sebagai indikator laboratorium untuk memantau trombositopenia dan respon trombopoiesis pada DBD.

Perbandingan Fraksi Trombosit Imatur (IPF) dan Prokalsitonin (PCT) sebagai Penanda Biologis Tingkat Keparahan Infeksi dalam Hubungannya dengan Antigen NS1 pada Pasien Demam Berdarah Dengue (DBD) Baihaki, Ichwan; -, Mutmainnah; Nugroho, Heru Purwanto; Aprilicia, Gita
Cermin Dunia Kedokteran Vol 53 No 06 (2026): Juni 2026
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v53i06.2035

Background: Dengue hemorrhagic fever (DHF) is an infectious disease caused by the dengue virus (DENV) and transmitted by the Aedes aegypti and Aedes albopictus mosquito vectors. This disease remains a major global health problem, particularly in tropical regions such as Indonesia. Clinical indicators of DHF severity include thrombocytopenia and shock, which are associated with high viremia levels and an increase in dengue NS1 antigen in the vascular system. This study compares immature platelet fraction (IPF) and procalcitonin (PCT) levels as biomarkers of infection severity in relation to DENV NS1 antigen positivity. Methods: This study included 35 patients with DHF and 35 healthy controls. IPF values and PCT levels were measured. Differences between the two groups were analyzed using an independent t-test. Results: p-values for both IPF and PCT were < 0.05, indicating statistically significant differences between DHF patients and healthy controls. These findings suggest that higher IPF values are associated with thrombocytopenia, whereas elevated PCT levels may reflect more severe infection, including dengue shock syndrome. Conclusion: There are significant differences in IPF and PCT levels between DHF patients and controls, depending on dengue NS1 antigen positivity. IPF may serve as an indicator of thrombocytopenia, while PCT may be a marker of infection severity, including dengue shock syndrome, in patients with dengue hemorrhagic fever.

Effectiveness of Mesenchymal Stem Cells and Their Derivatives in Modulating Oxidative Stress in Neurodegenerative Diseases: A Structured Narrative Review Niken Rahmah Ghanny; Putu Erma Pradnyani; Assyafiya Salwa; Herera Rahajeng; Afifa Radhina; Bunga Anggreini Sari; Ichwan Baihaki
Indonesian Journal of Life Sciences 2026: IJLS Vol 08 No.01
Publisher : Universitas Bio Scientia Internasional Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54250/ijls.v8i01.297

Background: Oxidative stress plays a critical role in the development and progression of neurodegenerative diseases such as Parkinson’s disease (PD), Alzheimer’s disease (AD), and amyotrophic lateral sclerosis (ALS). Mesenchymal stem cells (MSCs) and their derivatives have emerged as promising therapeutic strategies due to their antioxidant, anti-inflammatory, and neuroprotective properties. Objective: This review evaluated the effectiveness of MSC-based interventions in modulating oxidative stress in neurodegenerative disease models. Methods: A structured narrative review search was conducted following PRISMA 2020 guidelines using PubMed and Scopus databases for studies published between 2020 and 2025, with the last search in December 2025. Results: 33 studies met the inclusion criteria, primarily involving Parkinson’s and Alzheimer’s disease models. Overall, MSC-based therapies reduced oxidative stress markers, enhanced antioxidant defenses, activated the Nrf2/HO-1 pathway, improved mitochondrial function, and reduced neuroinflammation in experimental neurodegenerative disease models.

Beyond BMI in Community Health Promotion: An Integrated Anthropometric, Physiological, and Functional Screening Approach among Urban Women in East Jakarta, Indonesia Ichwan Baihaki; Taufiq Fredrik Pasiak; Afifa Radhina; Assyafiya Salwa; Bunga Anggreini Sari; Fransiskus Samuel Renaldi; Herera Rahajeng; Muhammad Fadhil; Niken Rahmah Ghanny; Ria Apriliani
Jurnal Ilmiah Pengabdian Masyarakat Bidang Kesehatan (Abdigermas) Vol. 4 No. 2 (2026): Jurnal Ilmiah Pengabdian Kepada Masyarakat Bidang Kesehatan (Abdigermas)
Publisher : CV Media Inti Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58723/abdigermas.v4i2.750

Background: Community-based preventive screening is important for reducing non-communicable disease risk among adult women, particularly those who influence household health practices. However, screening activities frequently emphasize Body Mass Index (BMI), although BMI alone may not adequately describe central adiposity, elevated blood pressure, and functional health indicators. This community service article aimed to describe the implementation of health education and integrated screening involving anthropometric, physiological, and functional measurements among female guardians at Yayasan Tarbiyatul Yatim, East Jakarta, Indonesia.Aims: This study aimed to (1) implement a community-based health education and screening program for adult female participants, (2) assess anthropometric, physiological, and functional health indicators including blood pressure, BMI, waist circumference, and hand grip strength, and (3) identify the prevalence of potential cardiometabolic and functional risk markers beyond BMI.Methods: This activity was implemented on January 8, 2026 using a preventive-promotive community service framework with descriptive program evaluation. Thirty-two adult female participants joined health education, screening, and individual consultation. The community service dataset included blood pressure, pulse, oxygen saturation, body temperature, body weight, height, BMI, waist circumference, hand grip strength, health history, lifestyle profile, and current complaints. Data were analyzed descriptively using frequency, percentage, mean, median, standard deviation, and range.Result: The participants had a mean age of 49.8 years. Mean systolic and diastolic blood pressure were 129.2 mmHg and 78.8 mmHg, respectively. Mean BMI was 24.9 kg/m²; 16/32 participants (50.0%) were categorized as normal, 9/32 (28.1%) overweight, 4/32 (12.5%) obese, and 3/32 (9.4%) underweight. Mean waist circumference was 97.7 cm, and 29/32 participants (90.6%) had waist circumference >80 cm. Mean hand grip strength was 16.5 kg, and 21/32 participants (65.6%) had values <18 kg.Conclusion: Integrated screening beyond BMI is feasible in community service settings and can help identify potential cardiometabolic and functional risk indicators that require counseling or referral. Future activities should include documented repeat measurements for abnormal findings, structured pretest-posttest educational evaluation, biochemical testing, and referral follow-up.

HISTOPATHOLOGICAL IMAGE OF THE NUMBER OF NEURONS IN THE BRAIN OF MICE (MUS MUSCULUS) INDUCED BY LEAD HEAVY METAL Herera Rahajeng; Eka Sri Haryati; Ichwan Baihaki; Niken Rahmah Ghanny; Afifa Radhina; Assyafiya Salwa; Fransiskus Samuel Renaldi
Journal of World Future Medicine, Health and Nursing Vol. 4 No. 2 (2026)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/health.v4i2.3677

Lead is a known heavy metal from human history to date for various uses, abuses and toxicities. Lead poisoning has been recognized as one of the most serious environmental health issues worldwide, especially children living in developing countries. Lead can damage or affect the organs of the body, among others, the nervous system, kidneys, reproductive system, and heart, as well as disturbances in the brain so that intellectual and mental disorders in the brain. This study aims to see the effect of lead on the number of neurons in the hippocampus in mice experimental animals. The mice used amounted to 10 tails and divided into two for the control group and the treatment group. The control group, each of which was 5 tails, was then given unbleached drinking water while the treatment group was given drinking water mixed with acetate with concentration of 1000ppm for 17 days in adlibitum. The observations were taken from each of the 5 fields of view on each preparation with a magnification of 40x. The calculation of the number of neuron cells is then analyzed descriptive statistics and different test. The result showed that control group with mean ± SD were 127,6 ± 7,9, and for treatment group that was 80,0 ± 20,9. T independent test result showed p = 0,001 (p <0,05) which means that there was a significant difference between control and treatment group. The conclusion of this research is the existence of neuron cell damage in mice that get treatment. By showing the number of neurons less than the control mice. Suggestion to develop this research hence need to continue research to find neuro protector compound specially from lead work in brain.

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