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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) IJENSET
Ramadhan Renaisansa
Akademi Farmasi Surabaya
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Electrical & Electronics Engineering Energy Engineering Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Physics

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Sabun Cair Cuci Tangan Berbasis Ekoenzim Inovatif dari Limbah Buah dan Sayur Menggunakan Gula Pasir dan Gula Aren: Solusi Antibakteri Berkelanjutan Melawan Bakteri Gram-Positif dan Gram-Negatif Pendukung SDGs dan Inisiatif Kesehatan Nasional Umarudin Umarudin; Ninik Mas Ulfa; Silvi Ayu Wulansari; Ramadhan Renaisansa; Selvyronica Eka Agustine; Aisyah Hadi Ramadani
Jurnal Penelitian Pendidikan IPA Vol 12 No 3 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i3.14595

Abstract

Indonesia's abundant fruit/vegetable waste threatens the environment yet fuels sustainable hygiene innovation. The waste will be fermented anaerobically with sugar and palm sugar. The study will also assess the soap's efficacy against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative). To achieve this, we produced an eco-enzyme by anaerobic fermentation. We then incorporated 5–10% of this enzyme directly into a pre-heated soap base. A hybrid hot-cold process was used: first, hot mixing ensured homogeneity; next, cold stabilisation was applied. pH testing and agar well diffusion assays followed. Results: Dose-response assays yielded significant (p < 0.05) increases in inhibition zone size; palm sugar at 10% excelled (35.3 mm E. coli, 23.6 mm S. aureus), surpassing commercial products. ANOVA followed by Tukey's post hoc (α = 0.05) showed that sugar variants (23.9 mm E. coli, 22.3 mm S. aureus) outperformed controls. pH: F0 8.83, F1 5.26, F3 4.79. Discussion: Remarkably, the exceptionally broad 35.3 mm E. coli zone reveals a groundbreaking novelty: unprecedented Gram-negative hyper susceptibility contrary to LPS resistance norms uniquely triggered by eco-enzyme organic acids synergising with palm sugar gallotannins to breach outer membranes. These zero-waste breakthroughs advance SDGs 3 and 12 by pioneering bio-circular antimicrobials.
Microbiological Contamination Profiling of Geothermal Hot Spring Water at Gambiran Padusan Bath, Pacet District, Mojokerto Regency Umarudin Umarudin; Ramadhan Renaisansa; Aisyah Hadi Ramadani
Indonesian Journal of Engineering, Science and Technology Vol. 3 No. 1 (2026): VOL. 03 NO. 01 (JUNE 2026)
Publisher : Universitas Muhammadiyah Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38040/ijenset.v3i1.1448

Abstract

This study provides an initial microbiological load assessment of Gambiran Padusan Bath hot spring water (36–39°C) using Total Plate Count (TPC) analysis based on ISO 4833-1:2013 standards. Pour plate technique on nutrient agar with 0.9% NaCl, across dilutions 10⁻¹ to 10⁻⁶, yielded 4.84 × 10⁴ CFU/mL from valid plates (25-250 colonies; averages: 219, 168.3, 126.3 CFU). Triplicate plating ensured reproducibility (RSD <10%, CV <15%), with declining counts in higher dilutions (17.7 to 0 CFU) confirming methodological reliability. Endogenous geothermal heat provides intrinsic disinfection, selectively favoring beneficial aerobes over pathogens, aligning TPC below WHO (<10⁵ CFU/mL) and BPAK 2017 recreational limits. Padusan's controlled microbiota poses minimal dermal/opportunistic infection risk during 15-20 min hydrotherapy sessions, supporting musculoskeletal relaxation (20-30% tension reduction), anti-inflammatory mineral absorption, and stress relief via endorphin induction. Logarithmic dilutions and 37±1°C incubation (mimicking skin temperature) optimized mesophile profiling, distinguishing therapeutic safety from bioprospecting. Negative 10⁻⁶ growth rules out hyper-contamination/aerosol risks. This baseline enables temporal monitoring, geotourism capacity planning (max 50 bathers/hour), and sustainable development, bridging public health with enzymatic bioprospecting potentials.   Keywords - Hot Spring; Hydrotherapy Safety; Microbial Load; Plate Count; Water Quality
Co-Authors Aisyah Hadi Ramadani Aisyah Hadi Ramadani Selvyronica Eka Agustine Ulfa, Ninik Mas Umarudin, Umarudin Wulansari, Silvi ayu