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All Journal EduMatika: Jurnal MIPA Journal of Education Technology Information Social Sciences and Health
Della Azzahra Batubara
Universitas Negeri Medan
Chemistry Computer Science & IT Education Mathematics Neuroscience Nursing Physics Public Health

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Application of bioethical aspects in the management of modified MS media waste in tissue culture laboratories M Rozan Pratama; Togi Miranda Pandiangan; Della Azzahra Batubara; Nurbaity Situmorang; Widya Arwita
EduMatika: Jurnal MIPA Vol. 6 No. 1 (2026): EduMatika: Jurnal MIPA
Publisher : Lembaga Riset Mutiara Akbar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56495/emju.v6i1.1563

Abstract

The development of biotechnology through plant tissue culture techniques provides significant benefits in producing superior, disease-free seedlings quickly. However, the use of modified Murashige and Skoog (MS) media also produces laboratory waste that has the potential to pollute the environment if not managed properly. This study aims to examine the management of tissue culture media waste, identify potential risks, and analyze the application of bioethical principles in its management. This study uses a descriptive qualitative approach with data collection techniques through observation and documentation in the Tissue Culture Laboratory. The results show that contaminated culture media waste is still disposed of directly without undergoing a sterilization process and waste separation according to standards. This condition has the potential to cause negative impacts such as the spread of microorganisms, environmental pollution due to chemical content, and the risk of health problems. From a bioethical perspective, this practice does not fully implement the principles of non-maleficence, beneficence, and responsibility. Therefore, the implementation of waste management in accordance with bioethical principles through sterilization, waste separation, and sustainable management is needed to support safe and responsible laboratory practices.
Perbanyakan Vegetatif Jambu Bol (Syzygium Malaccense) Melalui Teknik Sambung Pucuk Adinda Munthe; Della Azzahra Batubara; Mawar Gultom; Nursakinah Harahap; Rani Simanjuntak; Syalsabila Pane; Aswarina Nasution
Journal of Education Technology Information Social Sciences and Health Vol. 5 No. 2 (2026): September 2026
Publisher : CV. Rayyan Dwi Bharata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57235/jetish.v5i2.8438

Abstract

Guava (Syzygium malaccense) is a tropical fruit plant that has economic value and health benefits because it contains many active compounds that are good for the body. One way to vegetatively propagate guava is by using the grafting technique. This study aims to determine the process and success of the grafting technique on guava plants at the Horticultural Seed Development Unit of the Food Security, Agriculture, and Fisheries Service of Medan City and used a descriptive method through direct observation in the field. The selection of rootstock and scion, grafting procedures, binding the graft, and care of grafted seedlings are all part of the study. The results showed that the guava grafting technique was successful, with scion remaining fresh and new shoots emerging after the procedure. The accuracy of the cambium meeting between the rootstock and scion, the quality of the plant material, environmental conditions, and care after grafting are all factors that influence the success of grafting. Maintaining the superior traits of the parent plant, accelerating fruiting, producing uniform seedlings, and increasing plant resistance to environmental conditions, pests, and diseases are some of the advantages of this technique. Therefore, grafting can be an effective technique for producing superior guava seedlings.
Co-Authors Adinda Munthe Aswarina Nasution M Rozan Pratama Mawar Gultom Nurbaity Situmorang Nursakinah Harahap Rani Simanjuntak Syalsabila Pane Togi Miranda Pandiangan Widya Arwita