Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection

Sri Masyeni (Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Warmadewa, Bali, Indonesia Department of Internal Medicine, Sanjiwani Hospital, Bali, Indonesia)
Kuntaman Kuntaman (Department of Medical Microbiology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia Department of Medical Microbiology, Dr. Soetomo Hospital, Surabaya, Indonesia)
Aryati Aryati (Clinical Pathology Department, School of Medicine and Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia)
Muchlis AU Sofro (Department of Internal Medicine, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Department of Internal Medicine, Dr Kariadi Hospital, Semarang, Indonesia)
Usman Hadi (Department of Internal Medicine, Faculty of Medicine, Airlangga University, Surabaya, Indonesia Department of Internal Medicine, Dr. Soetomo Hospital, Surabaya, Indonesia)
Gondo Mastutik (Department of Anatomical Pathologic, Faculty of Medicine, Airlangga University, Surabaya, Indonesia)
Windu Purnomo (Department of Biostatistics, Faculty of Public Health, Universitas Airlangga Surabaya, Indonesia)
Agus Santosa (Departement of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Warmadewa, Bali, Indonesia)
Benediktus Yohan (Eijkman Institute for Molecular Biology, Jakarta, Indonesia)
Erni Juwita Nelwan (Division of Tropical and Infectious Disease, Department of Internal Medicine, Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia Infectious Disease and Immunology Research Center, Indonesia Medical and Education Research Institut)
R. Tedjo Sasmono (Eijkman Institute for Molecular Biology, Jakarta, Indonesia)

Narra J
Vol. 1 No. 1 (2021): April 2021

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.