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All Journal International Journal of Public Health Science (IJPHS) HAYATI Journal of Biosciences Jurnal Veteriner Jurnal Berkala Epidemiologi Indonesian Journal of Tropical and Infectious Disease Biology, Medicine, & Natural Product Chemistry Majalah Obstetri dan Ginekologi Dental Journal (Majalah Kedokteran Gigi) BIOMA Jurnal Respirasi (JR) Majalah Biomorfologi (Biomorphology Journal) Narra J Berkala Penelitian Hayati Journal of Clinical Microbiology and Infectious Diseases Prosiding Seminar Nasional COSMIC Kedokteran Strada Jurnal Ilmiah Kesehatan (SJIK) Folia Medica Indonesiana
Kuntaman Kuntaman
Faculty Of Medicine, Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Veterinary

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PROBLEM OF ANTIBIOTIC USE AND ANTIMICROBIAL RESISTANCE IN INDONESIA: ARE WE REALLY MAKING PROGRESS? Hadi, Usman; Kuntaman, Kuntaman; Qiptiyah, Mariyatul; Paraton, Hari
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.975 KB)

Abstract

Background: Based on the results Antimicrobial Resistance in Indonesia: prevalence and prevention-study (AMRIN-study), the Ministry of Health of Indonesia in 2005 began a program antibiotic resistance control (PPRA) in some government hospitals, and is currently developing to all government teaching hospitals in Indonesia. Aim: The core activities of the PPRA are to implement standardized surveillance emergence of antibiotic resistant bacteria, and the surveillance of antibiotic use in terms of quantity and quality. Method: Our research in the years 2003 showed the proportion of antibiotic use 84% of patients in a hospital. The use of inappropriate antibiotics was very high, 42% no indication. Result: In 2012 the results of surveillance showed decline of inappropriate use of antibiotic, but prevalence extended-spectrum b-lactamase (ESBL)-producing K.pneumoniae (58%), and E.coli (52%) andmethicillin-resistant S.aures (MRSA) (24%) were increasing. Conclusion: It was needed to implement the most appropriate programs to prevent the growth and development of bacteria resistant to antibiotics.
The Effectiveness of Audit Guideline Using Directive Discourse for Control and Prevention of Methicillin Resistant Staphylococcus aureus Infection Kusbaryanto Kusbaryanto; K. Kuntaman; WJ. Pudjirahardjo
International Journal of Public Health Science (IJPHS) Vol 5, No 1: March 2016
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.673 KB) | DOI: 10.11591/ijphs.v5i1.4766

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) infection is one of the major problems in the hospital, due to its increasing of prevalence. The aim of this study was to analyze the effectiveness of the Audit Guideline for control and prevention of MRSA infection, using directive discourse on nurses in hospitals. The Audit Guideline was focused on its effectiveness in improving the situation awareness of the nurses. This study was a quasi-experimental study using a pretest-posttest control group design. The population was ward nurses in a hospital. The samples were taken from four medical wards, two wards as the treatment group (25 nurses) and two wards as the control group (28 nurses). The two groups get the training of MRSA infection control and prevention. The training of the guideline of MRSA infection control and prevention using directive discourse was only applied in the treatment group. The analysis of the results of the situation awareness action was conducted, and the result of situation awareness action was improved from 0.8 response to 1.8 response (p = 0.014). This result was significantly difference because of the training using Audit Guideline for MRSA infections control and prevention with directive discourse become a positive reinforcement, the positive driven to stimulate behavior change of the nurses.
The Potency of Dextranase from Arthrobacter sp. Strain B7 as Dental Plaque Removal AFAF BAKTIR; NOOR CHOLIES ZAINI; UNTUNG MURDIYATMO; KUNTAMAN KUNTAMAN
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.646 KB) | DOI: 10.4308/hjb.12.4.162

Abstract

Dextranase of Arthrobacter sp. strain B7 (B7DEX enzyme) was characterized in this study. This enzyme hydrolyzed sucrose and dextran, but not other glucans (starch, nigeran, cellulose, -soluble glucan). It also hydrolyzed glucan from dental plaque with the activity of 7.38 +/- 66 U/ml, where the activity toward dextran was 31.88 +/- 1.24 U/ml. The enzyme exhibited the pH optimum of 7 and the temperature optimum of 50 0C. Its optimum stability was at pH 7 and 50 0C. The enzyme was inhibited by Fe3+, Cu2+, Zn2+, and Ag+, but not by the anionic detergent (SDS) and the nonionic detergent (Triton-X). The enzyme was activated by Ca2+, Na+, Mg2+, and saliva.
Prevalensi dan Pola Gen Extended Spectrum B-lactamase Bakteri Usus Sapi Perah dan Penduduk Sekitar Peternakan di Surabaya Triffit Imasari; Wiwiek Tyasningsih; Eddy Bagus Wasito; Kuntaman Kuntaman
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.766 KB)

Abstract

Since identified in 1980s, the multiple drug resistant organisms such as Extended Spectrum Beta Lactamases (ESBL) producing bacteria is increasing. These bacteria Enterobacteriaceae strain are mostly resistant against third and also fouth generation cephalosporin. ESBL-producing bacteria are identified in both of human, environment and also in animal. There are three main ESBL genes that are commonly found namely SHV, TEM and CTX-M. The aims of this study were to explore the prevalence and pattern of ESBL gene among dairy cows and people around the farm. The faecal samples were collected from dairy cows and people around the farm, cultured on MacConkey agar supplemented with cefotaxim 1 mg/L, incubated at 37oC for 24 hours. Then the growing colony were tested for ESBL producer by Double Disk Synergy Test (DDST), then followed by Polymerase Chain Reaction (PCR) for ESBL gene. Total sampling technique with inclusion and exclusion criteria, Total 49 samples were collected, consisting of 25 dairy cows faeces and 24 people faeces. Among these, were identified 18 samples (72%) positive in dairy cows and 19 samples (79.1%) positive results in the people around the dairy farm. The ESBL gene, SHV, TEM, CTX-M were identified dairy cows were zero for SHV, TEM (12%), CTX-M (72%) while in people around the farm SHV (25%), TEM (16.7%), CTX-M (66.7%). There were significant different (p < 0.05) between dairy cows and people around the farm, of SHV ESBL gene and not different (p>0.05) of TEM and CTX-M ESBL gene respectively. The ESBL genes have spread among dairy cows and people around the farm.
Pattern of disease and type of operation of Surgical Site Infection in obstetrics and gynecology at Dr Soetomo Hospital, Surabaya, Indonesia Ardi Eko Marsanto; Kuntaman Kuntaman; Hari Paraton; Budi Prasetyo
Majalah Obstetri dan Ginekologi Vol. 27 No. 2 (2019): August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.374 KB) | DOI: 10.20473/mog.V27I22019.49-55

Abstract

Objectives: to find correlation between risk factors and classification of SSI, the distribution of SSI, and cost in Soetomo Hospital.Materials and Methods: descriptive retrospective observational with correlation analysis between risk factors and SSI classification according to Centers for Disease Control (CDC) definition with univariate analysis. Sub-group descriptive analysis on microbiology result, length of stay (LOS) and cost was also performed. All patients was diagnosed SSI between January 2015 until June 2017.Results: age, referral cases, Body Mass Index, Hemoglobin and Albumin results, types of surgery, types of case are the risk factors we included. Analysis result shows no strong and significance correlation in all risk factors (r 0.053 – 0.447; p 0.072 – 0.971). According to SSI types, the distribution are: superficial (7; 13.2%), deep (26; 49.1%), organ/space (20; 37.7%). Extended-Spectrum Beta-Lactamase (ESBL) was obtained in 19 (14 Obstetrics; 5 Gynecology) from 28 patients with microbiology results. Five deaths occured in ESBL patients (1 Obstetrics; 4 Gynecology; ratio 1 : 11.2). Mean length of stay in Obstetrics and Gynecology was 41.7 and 19.2 days, respectively. Mean cost per day in Obstetrics and Gynecology was 1.2 amd 2.6 million rupiah, respectively.Conclusions: no strong and significant results in all risk factors. Death rate in SSI and malignancy with positive ESBL result are high. Less cost needed for SSI patients, but with longer LOS. Comprehensive approaches are needed to patients at risk of SSI, further study with larger sample are needed.
The role of inducible Nitric Oxide Synthase in teeth periapical lesions immunopathogenesis caused by Enterococcus faecalis Tamara Yuanita; Latief Mooduto; Kuntaman Kuntaman
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 1 (2013): March 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.797 KB) | DOI: 10.20473/j.djmkg.v46.i1.p14-17

Abstract

Background: Periapical lesions, are characterized by an immune response to the invading bacteria consequences periapical bone destruction. In root canal treatment failure was found Enterococcus faecalis (E. faecalis) as most species. iNOS found an important role in protection against infection, plays vital roles in fighting pathogens and contributing to disease pathology. Purpose: This study was to observed the role of iNOS in teeth periapical lessions immunopathogenesis caused by E. faecalis. Methods: The randomized post-only control group design used in this study, This study used 24 Wistar rats, were divided into three groups (each group consisted of 8 rats), as negative controls group is a normal teeth, in the positive controls group was made by drilling the upper right first molar to penetrate the dental pulp and was induced with 10µl BHI-b then filled with Glass Ionomer Cement (GIC) and the treatment group, after drilling the teeth, then inoculated with E. faecalis ATCC 29212 106 CFU into 10µl BHI-b then filled with GIC to prevent contamination. It takes 21 days to get periapical lesions and rat were sacrificed, and then the expression of iNOS was measured. Results: Statistical analysis using ANOVA found a significant differenced between control and treatment groups (p<0.05). Conclusion: This study concluded that iNOS role in teeth periapical lesions immunopathogenesis caused by E. faecalis.Latar belakang: Lesi periapikal merupakan hasil suatu respon imun untuk melawan invasi bakteri yang mengakibatkan destruksi tulang periapikal. Pada perawatan saluran akar yang mengalami kegagalan ditemukan Enterococcus faecalis sebagai spesies terbanyak. iNOS berperan penting untuk proteksi terhadap bakteri, mempunyai peran yang vital untuk melawan patogen dan berkonstribusi secara patologik untuk menyebabkan suatu penyakit. Tujuan: Penelitian ini bertujuan untuk mengobservasi peran iNOS secara imunohistokimia pada lesi periapikal tikus Wistar. Metode: Penelitian ini menggunakan disain randomized post-only control group, digunakan 24 ekor tikus Wistar yang dibagi menjadi tiga kelompok yang masing-masing terdiri dari 8 ekor tikus, sebagai kelompok kontrol negatif adalah gigi normal, pada kelompok kontrol positif dilakukan pengeboran pada gigi molar pertama rahang atas sampai menembus pulpa kemudian diinduksi 10µl BHI-b kemudian ditumpat (Glass Ionomer Cement) GIC dan pada kelompok perlakuan, setelah dilakukan pengeboran dilakukan induksi E. faecalis ATCC 29212 sebanyak 106 CFU ke dalam 10 µl BHI-b kemudian ditumpat GIC untuk mencegah kontaminasi. Diperlukan waktu 21 hari untuk mendapatkan lesi periapikal pasca perlakuan kemudian tikus dikorbankan lalu dihitung sel-sel yang mengekspresikan iNOS. Hasil: Analisis menggunakan ANAVA membuktikan bahwa ada perbedaan yang bermakna antara kelompok kontrol dan kelompok perlakuan (p<0,05). Kesimpulan: iNOS berperan pada imunopatogenesis lesi periapikal gigi akibat E. faecalis.
The distribution of Streptococcus mutans and Streptococcus sobrinus in children with dental caries severity level Nur Dianawati; Wahyu Setyarini; Ira Widjiastuti; Rini Devijanti Ridwan; K. Kuntaman
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 1 (2020): March 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i1.p36-39

Abstract

Background: The prevalence of dental caries is high worldwide and specifically in Indonesia, especially in children. Cariogenic bacteria are the major cause of dental caries. Streptococcus mutans (S. mutans) is one of the bacteria often associated with caries, due to its ability in producing acid and forming the biofilm for bacterial colonisation on the surface of oral cavities. In addition to S. mutans, Streptococcus sobrinus (S. sobrinus) bacteria are also thought to play an important role in the process of caries. Purpose: This study aims to analyse the distribution of S. mutans and S. sobrinus in children with seriously high dental caries levels. Methods: This study was an observational analytical study. Bacterial isolation was conducted in carious lesions of 50 paediatric patients 6-12 years old with superficial dental caries. Samples of caries lesions were put directly into a tube containing the Brain Heart Infusion Broth (BHI-B) and incubated at 37o C for 24 hours. The samples were sub-cultured on selective tryptone yeast cystine sucrose bacitracin (TYCSBHimedia) agar, and then incubated for two days. Bacterial identification was then performed using the polymerase chain reaction (PCR) Multiplex method. Statistical analysis with Chi-square. Results: The total number of children with dental caries included in this study was 50. Among these, 94% showed positive for S. mutans and 30% positive for S. sobrinus. The analysis of the prevalence of bacterial colonisation (S. mutans and S. sobrinus) based on caries severity and the Simplified Oral Hygiene Index (OHI-S), showed there was no significant difference (p> 0.05). Conclusion: This study showed that among 50 caries noted in the children, 94% were colonised S. mutans and 30% S. sobrinus. There was no significant difference between the colonisation of S. mutans and S. sobrinus among children from the severe to mild decayed exfoliated filling teeth (DEFT) category, and between bad and good OHI-S.
KEMAMPUAN TUMBUH Ecoli DH5 KOMPETEN DALAM 'MEDIUM MINIMAL' MENGANDUNG DEKSTRAN UNTUK MENGEMBANGKAN METODE SELEKSI KLON GEN DEKSTRANASE Afaf Baktir; Kuntaman Kuntaman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/510

Abstract

Dextranase gene cloning so far have used selection method base on halo formation around he recombinant dex colony grown on LB – blue dextran agar plate. The difficulty of the cloning process is in the selection of dex positive clone. As an example, for obtaining dex gene it has been screened about 36500 colonies. The reason that it was difficult to determine Dex positive clone because dextran hydrolysis by primary recombinant E.coli cells in LB – blue dextran medium was too weak. In the present research, we have designed a minimal medium contained dextran and low concentration of yeast extract to reduce difficulty and to increase accuracy and reproducibility determining of recombinant dex E.coli.In this experiment, dex positive cloned was simulated by competent E.coli grown in medium contained dextranase. The minimal medium designed consist of dextran 1% + yeast extract 0.01% +KH2PO4 0.1% + MgSO4 0.24% + NaCl 0.1% + CaCl2 0.01%. this medium have proved can distinguish between recombinant E.coli dex and other E.coli ie. The competent E.coli can grow well in this medium which was supplied by dextranase, but without dextranase this competent E.coli did not or limited grow
Clinical Microbiology Perspective of Empyema caused by Streptococcus constellatus in Malignancy Patient: A Case Report Agung Dewi Sekar; Rosantia Sarassari; Soedarsono Soedarsono; Kuntaman Kuntaman
Jurnal Respirasi Vol. 7 No. 2 (2021): May 2021
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.936 KB) | DOI: 10.20473/jr.v7-I.2.2021.75-78

Abstract

Introduction: Streptococcus constellatus (SC) is commensal bacteria and belongs to Streptococcus anginosus group (SAG). However, SC causes infections especially in patient with underlying diseases. SC empyema is a clinical case that is described in very few studies, especially in Indonesia.Case: A 45-year-old man was admitted to emergency department in Dr. Soetomo General Hospital Surabaya with respiratory insufficiency on November 11th 2020 after 3 months of non-productive cough and a week of weakness. He was diagnosed with empyema on right hemithorax and received chest tube insertion. SC was isolated from pleural fluid sample after first day of culture on Bactec BD bottle. GeneXpert result of pleural fluid was negative for Mycobacterium tuberculosis (MTB). He completed 10 days of intravenous ampicillin-sulbactam and metronidazole. The CT scan reported solid mass of 4.7x7.4x7.8 cm in posterolateral segment of inferior lobe right lung, right pleural effusion, with adenocarcinoma as biopsy result.Discussion: SC is a normal commensal in respiratory tract, however with the presence of a certain factor such as immunocompromised, colonized SAG directly induces an infection after entering normal sterile sites in the body including pleural fluid.Conclusion: Although infection caused by SC is a rare case, it still should be considered in clinical diagnosis and treatment of related infections, particularly in patients with comorbidities. The prognosis was good with appropriate antibiotics and chest tube insertion.
Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection Sri Masyeni; Kuntaman Kuntaman; Aryati Aryati; Muchlis AU Sofro; Usman Hadi; Gondo Mastutik; Windu Purnomo; Agus Santosa; Benediktus Yohan; Erni Juwita Nelwan; R. Tedjo Sasmono
Narra J Vol. 1 No. 1 (2021): April 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i1.31

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.
Co-Authors A. M. Widya A.A.W., I Wayan Putra Abdul Khairul Rizki Purba Mustofa Indwiani Astuti AFAF BAKTIR Agung Dewi Sekar Agus Santosa Agus Santosa akhmad sudibya, akhmad Alimsardjono, Lindawati Aquaresta, Febriana Ardani, I Gusti Wahju Ardhiya Puspita Ardi Eko Marsanto Arina Setyaningtyas Aryati , Aryati Aryati Aryati Aryati Aryati Bahraini, Fahrisah Nurfadeliah Benediktus Yohan Binti Mu’arofah Bramantono Bramantono, Bramantono Budi Prasetyo Budi Utomo Budiono Budiono Budiono Budiono Deby Kusumaningrum Dewi, Lisa Dharmawati, Ira Dian Neni Naelasari Dian Rachmawati Diani Dwi Indrasari Didik Hasmono Dwiyanti Puspitasari, Dwiyanti Eddy Bagus Wasito Eko B. Koendhori Eko Budi Khoendori Eko Budi Koendhori, Eko Budi Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Fourier Dzar Eljabbar Latief Gondo Mastutik Happy, Terza Aflika Hardiono Hardiono Hardiono Hardiono, Hardiono Hari Basuki Notobroto Hari Paraton Hari Paraton Hidayah, Ana Nurlaili Ida Bagus Narmada Iqhrammullah, Muhammad Ira Widjiastuti Irwanto Irwanto Ishak Samuel Wuwuti Kartuti Debora, Kartuti Kusbaryanto L. Alimsardjono Latief Mooduto, Latief Linda Dewanti Listyawati, Agusniar Furkani Ma'rifah, Nurul Maftuchah Maftuchah Margyaningsih, Nur Ita Maria Inge Lusida Maria Inge Lusida Marinda Dwi Puspitarini Mariyatul Qiptiyah, Mariyatul Marsheila Harvy Mustikaningtyas Masfufatun Masfufatun Masyeni, Sri Muchlis AU Sofro N. D. Kurniati Naritha Noor Cholies Zaini Nur Dianawati Nurul Ma'rifah P. N. Endraputra Parawidnyaningsih, Putu Ayu Aryanda Pristiawan Navy Endraputra Purnomo, Windu Puspitarini, Marinda Dwi Putu Arya Suryanditha R. Tedjo Sasmono Radita Yuniar Arizandy Rakhmatul Binti Sulistya Rasita, Yoeke Dewi Regita Cahyani, Nafansya Rini Devijanti Ridwan Risnanda, Kadek Rio Rosantia Sarassari Rosantia Sarassari Rosantia Sarassari Rosantia Sarassari S. R. S. Oktaviani S. Soedarsono Sacharissa Zerlina Tsarwah Thirafi Sarassari, Rosantia Sasmono, R. Tedjo Semedi, Bambang Pujo Setyarini, Wahyu Sholeh, Mohammad Akbaruddin Silvia Sutandhio Soekoyo, Agusta Reny Sofro, Muchlis AU Sofro, Muchlis AU. Sri Masyeni, Dewa Ayu Putri Sulistiawati Sulistiawati Sulistya, Rakhmatul Binti Syaiful, Irbasmantini Tamara Yuanita Terza Aflika Happy Thayyib, Muqoddar Triffit Imasari UNTUNG MURDIYATMO Usman Hadi Wahyu Setyarini Wahyu Setyorini Wahyu, Agung Dwi Wahyuni Dyah Parmasari, Wahyuni Dyah Windu Purnomo Wiwiek Tyasningsih WJ. Pudjirahardjo Yasmeen Lashari Yoeke Dewi Rasita Yohan, Benediktus