Biology, Medicine, & Natural Product Chemistry
Vol 15, No 1 (2026)

Identification of Salmonella spp. Contamination in Gado-Gado Sold Around the Tadulako University Campus

Pebi Angreni (Tadulako University)
I Nengah Kundera (Tadulako University)
Yulia Windarsih (Tadulako University)
Mohammad Jamhari (Tadulako University)
Vita Indri Febriani (Tadulako University)
Abdul Ashari (Tadulako University)



Article Info

Publish Date
01 Jun 2026

Abstract

Salmonella spp. are Gram-negative rod-shaped bacteria commonly found in the gastrointestinal tract of humans and animals and are recognized as one of the major causes of foodborne diseases. This study aimed to identify the presence of Salmonella spp. in gado-gado sold in the canteens of Tadulako University. This research employed a descriptive exploratory laboratory design. Samples were collected from five different canteens using a random sampling technique. Microbiological analyses were conducted through presumptive and confirmatory tests using the Most Probable Number (MPN) method, Total Plate Count (TPC), Gram staining, cultivation on selective Salmonella–Shigella Agar (SSA), and biochemical testing using Triple Sugar Iron Agar (TSIA). The MPN results showed an average value of 4,900 MPN/mL of sample, while the TPC reached 35,800/ml sample. These values indicate that the food product does not meet the microbiological safety standards established by the Indonesian Ministry of Health, as the TPC exceeds the maximum permissible limit of 104 CFU/mL.

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Journal Info

Abbrev

BIOMEDICH

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

Description

BIOLOGY, MEDICINE, & NATURAL PRODUCT CHEMISTRY, this journal is published to attract and disseminate innovative and expert findings in the fields of plant, animal, and microorganism secondary metabolite, and also the effect of natural product on biological system as a reference source for ...