The Influence of Breed and Age Differences on Serum Testosterone Levels of Male Cats in Pekanbaru, Riau, Indonesia

Mulya Fitranda (Universitas Riau)
Rifia Tiara Fani (Universitas Riau)
Tito Suprayoga (Universitas Riau)
Triawan Alkausar (Universitas Riau)
Frilianty Putri (Universitas Riau)
Hadimas Bakti Pratama (Universitas Riau)
Wiwin Suhandri (Universitas Riau)
Indra Jaya (Universitas Riau)
Bambang Saputra (Universitas Riau)
Chairunnisa Filzah Abdina (Universitas Riau)

Jurnal Sain Veteriner
Vol 44, No 2 (2026): Agustus

This study aimed to investigate the influence of breed and age on serum testosterone concentrations in intact male cats. A total of thirty (n=30) clinically healthy, intact male cats of various breeds and age groups were recruited from privately owned households in Pekanbaru, Riau Province, Indonesia. Information on age and breed was obtained from structured owner questionnaires. Blood samples were collected aseptically from the jugular vein and processed to obtain serum, which was stored at –20°C until analysis. Serum testosterone concentrations were measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method, and data were statistically analyzed using IBM SPSS Statistics software with a significance level of α = 0.05 (95% confidence interval). The study revealed a significant difference between age and serum testosterone levels. In contrast, no significant difference was found between breed and testosterone concentration. These findings provide preliminary reference data for future research in feline reproductive endocrinology and may serve as a foundation for assessing androgenic function in domestic cats.