Jurnal Ventilator: Jurnal Riset Ilmu Kesehatan dan Keperawatan
Vol. 4 No. 2 (2026): Juni : Jurnal Ventilator

Kajian Komparatif Kadar Metabolit Sekunder Saponin pada Anggur Laut (Caulerpa racemosa) : Analisis Variasi Konsentrasi Etanol dengan Metode Spektrofotometri

Ayummi Halimah Dhyani (Universitas Bengkulu)
Dwi Kurnia Putri (Universitas Bengkulu)
Tri Danang Kurniawan (Universitas Bengkulu)
Rose Intan Perma Sari (Universitas Bengkulu)
Oky Hermansyah (Universitas Bengkulu)



Article Info

Publish Date
01 Jun 2026

Abstract

Sea grapes (Caulerpa racemosa) are green algae that contain secondary metabolites, including saponins, which have potential as bioactive compounds. This study aimed to determine the saponin content in 70% and 96% ethanol extracts and to analyze the differences in saponin levels between the two solvents using the UV-Vis spectrophotometric method.This research was an experimental laboratory study involving sample preparation, extraction by maceration using 70% and 96% ethanol, qualitative saponin testing, and quantitative determination of saponin levels using the vanillin–sulfuric acid method. Measurements were carried out at a maximum wavelength of 495 nm using a standard calibration curve with concentrations of 20–100 ppm.The results showed that the saponin content in the 70% ethanol extract was 954.06 ± 11.0 mg QE/g, while in the 96% ethanol extract it was 938.06 ± 8.9 mg QE/g. Statistical analysis using an independent t-test indicated no significant difference between the two extracts (p > 0.05).In conclusion, both 70% and 96% ethanol solvents are capable of extracting saponins from Caulerpa racemosa, but there is no significant difference in the saponin content obtained.  

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Journal Info

Abbrev

Ventilator

Publisher

Subject

Health Professions Nursing Public Health

Description

Bidang kesehatan dan keperawatan termasuk penelitian dasar dalamkesehatan dan keperawatan, keperawatan manajemen, keadaan darurat, dan keperawatan kritis, keperawatan medis-bedah, keperawatan kesehatan mental, keperawatan maternitas, keperawatan Anak, gerontologis keperawatan, keperawatan komunitas, ...