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All Journal Jurnal Ventilator: Jurnal Riset Ilmu Kesehatan dan Keperawatan
Ayummi Halimah Dhyani
Universitas Bengkulu
Health Professions Nursing Public Health

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Kajian Komparatif Kadar Metabolit Sekunder Saponin pada Anggur Laut (Caulerpa racemosa) : Analisis Variasi Konsentrasi Etanol dengan Metode Spektrofotometri Ayummi Halimah Dhyani; Dwi Kurnia Putri; Tri Danang Kurniawan; Rose Intan Perma Sari; Oky Hermansyah
Jurnal Ventilator Vol. 4 No. 2 (2026): Juni : Jurnal Ventilator
Publisher : Stikes Kesdam IV/Diponegoro Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59680/ventilator.v4i1.2370

Abstract

Sea grapes (Caulerpa racemosa) are green algae that contain secondary metabolites, including saponins, which have potential as bioactive compounds. This study aimed to determine the saponin content in 70% and 96% ethanol extracts and to analyze the differences in saponin levels between the two solvents using the UV-Vis spectrophotometric method.This research was an experimental laboratory study involving sample preparation, extraction by maceration using 70% and 96% ethanol, qualitative saponin testing, and quantitative determination of saponin levels using the vanillin–sulfuric acid method. Measurements were carried out at a maximum wavelength of 495 nm using a standard calibration curve with concentrations of 20–100 ppm.The results showed that the saponin content in the 70% ethanol extract was 954.06 ± 11.0 mg QE/g, while in the 96% ethanol extract it was 938.06 ± 8.9 mg QE/g. Statistical analysis using an independent t-test indicated no significant difference between the two extracts (p > 0.05).In conclusion, both 70% and 96% ethanol solvents are capable of extracting saponins from Caulerpa racemosa, but there is no significant difference in the saponin content obtained.  
Co-Authors Dwi Kurnia Putri Oky Hermansyah Rose Intan Perma Sari Tri Danang Kurniawan