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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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Articles 34 Documents
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Search results for , issue "Vol. 6 No. 2 (2019): December 2019" : 34 Documents clear
Front Cover JBBI Vol 6, No 2, December 2019 ., .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.075 KB) | DOI: 10.29122/jbbi.v6i2.3998

Abstract

PENGARUH SEREAL BERBAHAN SAGU DAN Moringa oleifera TERHADAP KADAR GLUKOSA DARAH TIKUS YANG DIINDUKSI ALOKSAN Herada Putri, Melin Novidinisa; Fauziyah, A'immatul; Maryusman, Taufik
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.063 KB) | DOI: 10.29122/jbbi.v6i2.3669

Abstract

The Effects of Cereal Made From Sagu and Moringa oleifera on the Blood Glucose Level of Alloxan-Induced RatsDiabetes Mellitus (DM) type 2 could increase oxidative stress and blood glucose level. Resistant starch compounds in instant cereal Cersa Mori have antidiabetic properties. This research aimed to analyze the effect of Cersa Mori on fasting blood glucose (FBG) levels of diabetic white rats induced by alloxan. This is a true experimental study with a randomized pre-post control group design using 27 male Wistar strain rats divided into 3 groups randomly, i.e (KN) feed and distilled water, (KP) glibenclamide 0.126mg/200gBB/day, (P) Cersa Mori 5g/200gBB/day. KP and P groups were given alloxan 125 mg/KgBB subcutaneously and the intervention was carried out for 30 days. FBG level was measured using the GOD-PAP method. The results of Paired T-Test showed the effect of Cersa Mori on lowering FBG levels in hyperglycemic rats (P = 0,006). One-Way ANOVA test showed that Cersa Mori reduced FBG level, which was equivalent to those given glibenclamide (P = 0,366). It can be concluded that giving Cersa Mori 5g/200gBB/day for 30 days had a significant effect on lowering FBG level. Keywords: alloxan; Cersa Mori; diabetic rats; fasting blood glucose level; resistant strachABSTRAKDiabetes Mellitus (DM)tipe 2 dapat memicu stres oksidatif dan meningkatkan kadar glukosa darah puasa (GDP). Senyawa pati resisten dalam sereal siap saji Cersa Mori memiliki sifat antidiabetik. Penelitian ini bertujuan menganalisis pengaruh pemberian Cersa Mori terhadap kadar glukosa darah tikus putih diabetes yang diinduksi aloksan. Penelitian true-experimental ini menggunakan randomized pre-post control group design. Sampel sebanyak 27 ekor tikus jantan galur Wistar dibagi menjadi 3 kelompok secara acak yaitu; (KN) pakan dan akuades, (KP) glibenklamid 0,126 mg/200gBB/hari, (P) Cersa Mori 5g/200gBB/hari. KP sampai P diberikan aloksan 125 mg/KgBB secara subkutan dan intervensi dilakukan selama 30 hari. Pengukuran GDP menggunakan metode GOD-PAP. Hasil Uji-T menunjukkan pengaruh Cersa Mori dalam menurunkan GDP tikus hiperglikemia (P = 0,006). Uji ANOVA satu arah menunjukkan penurunan GDP pada kelompok Cersa Mori (P) setara dengan tikus yang diberi glibenklamid (P = 0,366). Dengan demikian dapat disimpulkan bahwa pemberian Cersa Mori dosis 5g/200gBB/hari selama 30 hari berpengaruh terhadap penurunan GDP secara signifikan.
SKRINING DAN ANALISIS FITOKIMIA TUMBUHAN OBAT TRADISIONAL MASYARAKAT KABUPATEN BIMA Azmin, Nikman; Rahmawati, Anita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1113.415 KB) | DOI: 10.29122/jbbi.v6i2.3504

Abstract

Phytochemical Screening and Analysis of Traditional Herbal Medicines of Bima DistrictBima people use medicinal plants for traditional medicine which is local wisdom that must be preserved. Thus, the phytochemical content of these plants needs to be studied. This study aims to determine the secondary metabolite content of traditional medicinal plants in order to enrich biotechnology and pharmacological data. Thus, the data can be used as a reference in healing diseases and managing various medicinal plants based on community welfare and environmental sustainability for the people of Bima regency. The method used is a detailed interview and a field survey followed by phytochemical screening to determine the content of compounds in plants. From the results of this study, as many as 17 types of medicinal plants have been used as traditional medicine. The plant parts used are leaves, stems, flowers, roots, rhizomes, fruit, gum or lenders with 33% weed habitus followed by herbs (29%), trees (29%), and shrubs (9%). The compounds identified in these medicinal plants are flavonoids, alkaloids, steroids, terpenoids, saponins, and tannins.Keywords: Bima regency; local wisdom; medicinal plants; phytochemicals; secondary metabolitesABSTRAKMasyarakat Bima memanfaatkan tumbuhan obat untuk pengobatan tradisional yang merupakan kearifan lokal yang harus dipertahankan, sehingga kandungan fitokimia dari tumbuhan-tumbuhan ini perlu diteliti. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder tumbuhan obat tradisional guna memperkaya data bioteknologi dan farmakologi. Dengan demikian, data tersebut dapat dijadikan acuan dalam penyembuhan penyakit dan pengelolaan berbagai tumbuhan obat berbasis kesejahteraan masyarakat dan kelestarian lingkungan bagi masyarakat kabupaten Bima. Metode yang digunakan adalah wawancara secara rinci dan survei lapangan dilanjutkan dengan skrining fitokimia untuk mengetahui kandungan senyawa dalam tumbuhan. Dari hasil penelitian ini diketahui sebanyak 17 jenis tumbuhan obat yang telah dimanfaatkan sebagai obat tradisional. Bagian tumbuhan yang digunakan berupa daun, batang, bunga, akar, rimpang, buah, getah atau lender dengan jenis habitus 33% gulma diikuti oleh herbal (29%), pohon (29%), dan perdu (9%). Sedangkan senyawa yang teridentifikasi dalam tumbuhan obat tersebut yaitu flavonoid, alkaloid, steroid, terpenoid, saponin, dan tannin.
OPTIMASI PERMUKAAN RESPONS MEDIUM FERMENTASI Streptomyces prasinopilosus SEBAGAI ANTIFUNGI TERHADAP PATOGEN Ganoderma boninense Sunaryanto, Rofiq; Nurani, Diana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1326.321 KB) | DOI: 10.29122/jbbi.v6i2.3231

Abstract

Response Surface Optimization of Medium Fermentation for Streptomyces prasinopilosus as An Antifungal against Ganoderma boninenseGanoderma boninense is one of the pathogenic fungi that cause basal stem rot (BPB) on oil palm plants. This research aims to study the effect of carbon sources, nitrogen sources, and minerals on the production of Streptomyces prasinopilosus active compounds. Lactose, yeast extract, and minerals are medium components that show a real influence on the production of S. prasinopilosus active compounds. Optimization of the factors that have significant influence was predicted by the second-order model, statistically through a central composite design (CCD). The highest S. prasinopilosus active compound production, with a medium composition of 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution, was predicted by the quadratic model to reach 32269366.338 peak area unit on high-performance liquid chromatography (HPLC). The verification of the mathematical model of the production of the active compounds through experiments in the laboratory was 27,203,907.310 peak area unit. This result was 15.7% lower compared to the result of the quadratic model. Optimization increased S. prasinopilosus active compound 9-fold compared to that before optimization.Keywords: active compound; G. boninense; optimization; RSM; S. prasinopilosus ABSTRAKGanoderma boninense merupakan salah satu jamur patogen yang menyebabkan penyakit busuk pangkal batang atau biasa disebut BPB pada tanaman kelapa sawit. Penelitian bertujuan mempelajari pengaruh sumber karbon, sumber nitrogen, dan mineral terhadap produksi senyawa aktif S. prasinopilosus. Laktosa, yeast extract, dan mineral adalah komponen medium yang menunjukkan pengaruh nyata terhadap produksi senyawa aktif S. prasinopilosus. Optimasi terhadap faktor yang berpengaruh nyata diprediksi dengan model orde dua melalui rancangan statistis central composite design (CCD). Produksi senyawa aktif S. prasinopilosus tertinggi diprediksi oleh model kuadratik mencapai 32269366,338 luasan puncak kromatografi cair kinerja tinggi (KCKT) dengan komposisi medium laktosa 44,77 g L-1, yeast extract 13,02 g L-1, dan larutan mineral 15,95 mL L-1. Verifikasi model matematis produksi senyawa aktif yang dihasilkan melalui percobaan di laboratorium adalah sebesar 27.203.907,310 luasan puncak kromatogram KCKT. Hasil ini lebih rendah 15,7% dibandingkan dengan model kuadratik hasil optimasi. Optimasi meningkatkan senyawa aktif S. prasinopilosus 9 kali lipat dibandingkan sebelum optimasi.
Back Cover JBBI Vol 6, No 2, December 2019 ., .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.157 KB) | DOI: 10.29122/jbbi.v6i2.3999

Abstract

PENGARUH MEDIA DASAR DAN NAA PADA INDUKSI IN VITRO AKAR TUNAS KELAPA SAWIT (Elaeis guineensis) Karyanti, .; Afifah, Mutia; Sukarnih, Tati; Rudiyana, Yayan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (660.696 KB) | DOI: 10.29122/jbbi.v6i2.3476

Abstract

The Effect of Basal Media and NAA on the In Vitro Induction of Oil Palm (Elaeis guineensis Jacq.) RootABSTRACTClonal propagation of oil palm plants using tissue culture technique results in a low percentage of rooted shoots. To increase the percentage of rooted shoots that are more uniform, the root induction method is supported by the use of basic media and the addition of growth regulators 1-naphthaleneacetic acid (NAA). This study aims to analyze the effect of a combination of base media and optimum NAA concentration in inducing the roots of oil palm shoots in vitro. This research used factorial completely randomized design (RAL) consisting of 2 factors. The first factor was the type of basic media, namely Murashige and Skoog (MS) and MS Modifications (MSM) media. The second factor was the concentration of NAA, namely 0; 0.05; 0.1; and 0.2 ppm. each treatment was repeated 10 times. The results showed that the use of MSM medium was better than that of MS, and the most optimum NAA concentration was 0.05 and 0.1 ppm, in inducing oil palm roots in vitro. In addition, the combination of MSM + NAA 0.1 ppm treatment produced the most optimum result in induction of oil palm roots in vitro.Keywords: basal media; NAA; palm oil; plantlet; root inductionABSTRAKPerbanyakan klonal tanaman kelapa sawit menggunakan teknik kultur jaringan menghasilkan persentase tunas berakar yang rendah. Dalam upaya meningkatkan persentase tunas berakar yang lebih seragam maka dilakukan metode induksi akar yang didukung oleh penggunaan media dasar dan penambahan zat pengatur tumbuh 1-naphthaleneacetic acid (NAA). Penelitian ini bertujuan untuk menganalisis pengaruh kombinasi media dasar dan konsentrasi NAA yang optimum dalam menginduksi akar tunas kelapa sawit secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari 2 faktor. Faktor pertama adalah jenis media dasar yang terdiri dari media Murashige and Skoog (MS) dan MS Modifikasi (MSM). Faktor kedua adalah konsentrasi NAA yang terdiri dari 0; 0,05; 0,1; dan 0,2 ppm. setiap perlakuan diulang sebanyak 10 kali. Hasil penelitian menunjukkan bahwa penggunaan media MSM lebih baik daripada MS, dan konsentrasi NAA yang paling optimum adalah 0,05 dan 0, 1 ppm dalam menginduksi akar kelapa sawit secara in vitro. Selain itu kombinasi perlakuan MSM+NAA 0,1 ppm memiliki hasil yang paling optimum dalam induksi akar kelapa sawit secara in vitro.
PENGARUH KONSENTRASI NANOPARTIKEL PERAK IONIK TERHADAP DAYA HAMBAT BAKTERI Lactobacillus casei DAN pH SUSU Vegisari, Vegisari; Suparno, Suparno; Rany, Tiara Delvika
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.495 KB) | DOI: 10.29122/jbbi.v6i2.3506

Abstract

The Effect of Ionic Silver Nanoparticles Concentrations on the Inhibition of Lactobacillus casei and pH of MilkABSTRACTTechnological developments support the creation of various nanoparticles that can be utilized, one of which is silver nanoparticles. This study aims to determine the effect of ionic silver nanoparticle concentration on Lactobacillus casei growth inhibition, pH change and curdling of UHT milk. Variation in the concentration of ionic silver nanoparticles of 5, 10, 15, and 20 ppm was tested for their characteristics through UV-VIS spectrophotometry, showing the wave peaks at 413–430 nm, and through the PSA test resulting in the Z-average of 87.2 nm. Ionic silver nanoparticles were tested against L. casei using the Kirby-Bauer diffusion method for 72 hours. The test results showed a change in the clear zone that tended to increase for the concentration of silver nanoparticles 5, 10, 15 and 20 ppm with the gradient values of 0,0032; 0,0280; 0,0395; dan 0,0317, respectively. Tests for pH and UHT milk curdling were carried out for 78 hours and showed that ionic silver nanoparticles tended to be more able to maintain milk pH ±30 hours and curdling ±24 hours longer than milk that was not treated with ionic silver nanoparticles.Keywords: electrolysis; Lactobacillus casei; milk; nanoparticles; silver ABSTRAKPerkembangan teknologi mendukung terciptanya berbagai nanopartikel yang dapat dimanfaatkan, salah satunya nanopartikel perak. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi nanopartikel perak ionik terhadap daya hambat Lactobacillus casei, kadar pH, dan penggumpalan pada susu UHT. Variasi konsentrasi nanopartikel perak ionik 5, 10, 15, dan 20 ppm diuji karakteristiknya melalui spektrofotometri UV-VIS dengan hasil puncak gelombang serapan berada pada kisaran 413–430 nm dan uji PSA dengan hasil Z-average berada pada 87,2 nm. Sampel larutan nanopartikel perak ionik diujikan terhadap bakteri L. casei menggunakan metode difusi Kirby-Bauer selama 72 jam. Hasil uji menunjukkan terjadinya perubahan zona bening yang cenderung meningkat untuk konsentrasi nanopartikel perak 5, 10, 15 dan 20 ppm dengan masing-masing nilai gradiennya 0,0032; 0,0280; 0,0395 dan 0,0317. Pengaruhnya terhadap kadar pH dan penggumpalan susu UHT dilakukan selama 78 jam dan menunjukkan bahwa nanopartikel perak ionik cenderung lebih mampu mempertahankan pH susu ±30 jam dan penggumpalan ±24 jam lebih lama dibanding susu yang tidak diberikan perlakuan nanopartikel perak ionik.
OPTIMASI TEKNIK WESTERN BLOT UNTUK DETEKSI EKSPRESI PROTEIN TANAMAN PADI (Oryza sativa L.) Susianti, .; Sukmana, Edi; Lesmana, Ronny; Supratman, Unang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.722 KB) | DOI: 10.29122/jbbi.v6i2.3249

Abstract

Optimization of Western Blot Technique for Protein Expression of Rice Plant (Oryza sativa L.) Western blot (WB) technique has been widely used for analyzing protein expression and for identifying specific proteins derived from animals, plants, and microorganisms. During the use of WB, especially in agricultural studies, some difficulties are encountered such as unclear or unspecific protein bands, the presence of bubbles, and the absence of protein bands on membrane. This study aims to determine the WB conditions appropriate for the protein expression of rice plants (Oryza sativa L.). Protein from rice plant was extracted and the obtained protein lysate was then used for proteomic analysis using western blot with β-actin antibody. Our experiment showed that some optimized parameters like blocking buffers, the concentration of primary antibody and the ratio of secondary antibody determined the clarity of the results. β-actin was used as internal control that measured the success of the WB technique. Results showed that lysis process was important in determining good WB results in addition to the optimal blocking solution using a BSA of 0.2%, a primary antibody concentration of 1 μg mL–1, and a secondary antibody of 1:10,000. Optimizing techniques during extraction, incubation, and documentation facilitated good WB results.Keywords: β-actin; optimization; protein; rice plant; western blotABSTRAKTeknik western blot (WB) telah banyak digunakan untuk analisis ekspresi protein dan mengidentifikasi protein spesifik dari hewan, tumbuhan dan mikroorganisme. Dalam implementasi teknik WB, khususnya studi dalam bidang pertanian, beberapa kesulitan ditemui seperti pita protein tidak jelas, tidak spesifik, adanya gelembung, hingga tidak munculnya pita protein pada membran. Penelitian ini bertujuan untuk mengetahui kondisi WB yang tepat untuk deteksi protein tanaman padi (Oryza sativa L.). Protein tanaman padi diekstraksi, kemudian lysate protein yang didapat dianalisis dengan metode westernblot menggunakan antibody β-actin. Penelitian kami menunjukkan bahwa beberapa parameter yang dioptimasi seperti larutan blocking, konsentrasi antibodi primer dan rasio antibodi sekunder akan menentukan hasil yang jelas. β-actin digunakan sebagai kontrol internal yang menjadi tolok ukur keberhasilan teknik WB. Hasil menunjukkan bahwa proses lisis menjadi hal penting dalam menentukan hasil WB yang baik disamping larutan blocking yang optimal menggunakan BSA 0,2%, konsentrasi antibodi primer 1 µg mL–1 dan antibodi sekunder 1:10.000. Mengoptimalkan teknik selama ekstraksi, inkubasi dan dokumentasi membantu mendapatkan hasil WB yang baik.
Preface JBBI Vol 6, No 2, December 2019: Foreword and Acknowledgement ., .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.043 KB) | DOI: 10.29122/jbbi.v6i2.4000

Abstract

Appendix JBBI Vol 6, No 2, December 2019: Keyword Index and Author Index ., .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.366 KB) | DOI: 10.29122/jbbi.v6i2.4006

Abstract

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