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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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Search results for , issue "Vol. 8 No. 2 (2021): December 2021" : 18 Documents clear
POTENSI KOMBUCHA DAUN TEH (Camellia sinensis) DAN DAUN KOPI ROBUSTA (Coffea robusta) SEBAGAI MINUMAN PROBIOTIK Elok Zubaidah; Kiki Fibrianto; Soviandini Dwiki Kartikaputri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.334 KB) | DOI: 10.29122/jbbi.v8i2.4186

Abstract

Kombucha is a plant-based fermented beverage that contains probiotic bacteria such as lactic acid bacteria (LAB). This research was conducted to prove the potential of probiotics in LAB isolates of kombucha tea leaves and robusta coffee leaves with various concentrations. This research used a completely randomized design (CRD). The variables tested were the measurement of total lactic acid bacteria, total acid, and probiotic characterization of LAB isolates (LAB resistance to low pH and bile salts, and antibacterial activity test). The results were analyzed and discussed using ANOVA with a significance level of P < 0.05 only for total acid and descriptive analysis on microbiological response. The best probiotic potency was selected using the multiple attribute method. The results showed that LAB isolates from kombucha tea leaves and robusta coffee leaves with various concentrations had resistance to pH 2 and pH 3, resistance to bile salts 3%, and antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria. The LAB isolate of kombucha robusta coffee leaves at a concentration of 0.6% has the best probiotic potential. Kombucha merupakan minuman fermentasi berbasis tanaman yang mengandung bakteri probiotik seperti bakteri asam laktat (BAL). Penelitian ini dilakukan untuk membuktikan potensi probiotik pada isolat BAL kombucha daun teh dan daun kopi robusta dengan berbagai konsentrasi. Penelitian laboratorium menggunakan rancangan acak lengkap (RAL). Variabel yang diuji adalah pengukuran total bakteri asam laktat, total asam, ketahanan BAL terhadap pH rendah dan garam empedu, serta uji aktivitas antibakteri. Hasil penelitian dianalisa dan dibahas menggunakan ANOVA dengan signifikansi P < 0.05 hanya untuk total asam dan analisa deskriptif pada respons mikrobiologis. Potensi probiotik terbaik dipilih menggunakan metode multiple attribute. Hasil penelitian menunjukkan bahwa isolat BAL dari kombucha daun teh dan daun kopi robusta dengan berbagai konsentrasi memiliki ketahanan terhadap pH 2 dan pH 3, ketahanan terhadap garam empedu 3%, serta aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Isolat BAL kombucha daun kopi robusta pada konsentrasi 0,6% mempunyai potensi probiotik terbaik.
KARAKTERISASI BERBASIS MARKA MOLEKULER ITS2 TERHADAP SUB-SPESIES KOMPLEKS Anopheles vagus vagus DAN Anopheles vagus limosus Kartika Senjarini; Lailly Nur Uswatul Hasanah; Miatin Alvin Septianasari; Muhammad Khalid Abdullah; Rike Oktarianti; Syubbanul Wathon
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.15 KB) | DOI: 10.29122/jbbi.v8i2.4737

Abstract

The presence of intraspecies variations of An. vagus later categorized as the subspecies of An. vagus vagus and An. vagus limosus, could be an obstacle to the identification process, which is an important step for malaria vector’s competence characterization. Based on morphological identification, those subspecies could be distinguished by the presences of pale scales in prehumeral and pale bands  in proboscis. The objective of this research was to compare subspecies complexes of An. vagus morphologically and molecularly using Internal Transcribed Spacer 2 (ITS2). Anopheles samples were collected from Bangsring, Banyuwangi. Their phylogenetic tree was constructed by using NJ method based on their ITS2 sequences. BLAST result showed that An. vagus vagus and An. vagus limosus were similar to An. vagus FJ654649.1 from East Java Indonesia and East Timor based on its 99% homology and their molecular distance. The Neighbour Joining (NJ) tree grouped those subspecies in one clade with a boostrap value of 82%. This subspeciation might be due to the different rates of evolution. ITS2 sequences of An. vagus vagus and An. vagus limosus were submitted to GenBank with the accession number of MW314227.1 and MW319822.1, respectively. Kemunculan variasi intraspesies An. vagus yang kemudian dikategorikan sebagai subspesies An. vagus vagus dan An. vagus limosus menjadi kendala dalam proses identifikasi yang merupakan langkah penting dalam menentukan kompetensi vektor malaria. Berdasarkan karakter morfologi, subspesies tersebut dibedakan dengan adanya sisik pucat pada bagian prehumeral dan pita pucat pada probosis. Penelitian ini bertujuan untuk membandingkan subspesies An. vagus secara morfologis dan molekuler menggunakan Internal Transcribed Spacer 2 (ITS2). Nyamuk Anopheles didapatkan dari Bangsring, Banyuwangi. Konstruksi pohon filogeni dilakukan berdasarkan sekuen ITS2 yang dianalisis menggunakan metode NJ. Hasil BLAST menunjukkan, ITS2 An. vagus vagus dan An. vagus limosus memiliki tingkat homologi 99% dan jarak evolusi molekuler terendah dengan An. vagus FJ654649.1 dari Jawa Timur Indonesia dan Timor Timur. Pohon NJ mengelompokkan subspesies tersebut dalam satu klade dengan nilai boostrap 82%. Hal ini dapat terjadi karena perbedaan kecepatan evolusi yang memungkinkan terjadinya subspesiasi. Urutan basa ITS2 dari An. vagus vagus dan An. vagus limosus telah didaftarkan ke GenBank dengan nomor aksesi MW314227.1 dan MW319822.1.
SSR MARKERS CHARACTERIZATION FOR TEMU IRENG (Curcuma aeruginosa Roxb.) GENERATED FROM EST OF Curcuma longa Devit Purwoko; Siti Zulaeha; Teuku Tajuddin; Hayat Khairiyah; Reynaldi Zulfikar Fauzi; . Priyanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.554 KB) | DOI: 10.29122/jbbi.v8i2.4763

Abstract

Temu ireng (Curcuma aeruginosa) is used as a traditional herb medicine in Indonesia. Expressed Sequence Tags (EST) of C. longa was used because there were no genome data of C. aeruginosa. This study aimed to recognize the diversity of SSR characteristic and to develop a primer for C. aeruginosa genetic diversity. The EST was preprocessed and analyzed fogg7r the SSR motif. Primers were designed using SSR motifs excluding mononucleotide (? 20 bp) and analyzed based on gene ontology. The result showed that out of 12.675 EST used there were 3.005 contig and 548 perfect SSR motif with the motif frequency of 1/15,27 kb sequences achieved. AGG trinucleotide was mostly distributed (37,40%). There were 380 primers designed and 10 primers validated by PCR on three C. aeruginosa clones from Ponorogo, Cikarang, and Kebumen. The number of alleles detected was about 2 to 3 per locus. The polymorphic microsatellite markers produced from this study could be used for the analysis of genetic diversity of C. aeruginosa in Indonesia. Temu ireng (Curcuma aeruginosa) digunakan sebagai bahan baku obat herbal tradisional di Indonesia. Expressed Sequence Tags (EST) dari C. longa digunakan karena sampai saat ini belum adanya data genome dari C. aeruginosa. Penelitian ini bertujuan untuk mengetahui keragaman karakteristik SSR dan mengembangkan primer untuk prastudi keragaman genetik C. aeruginosa. EST dilakukan prapemrosesan dan dianalisis motif SSR. Primer didesain menggunakan motif SSR selain mononukleotida (? 20 bp) dan dianalisis berdasarkan ontologi gen. Hasil yang diperoleh dari 12.678 EST yang digunakan dihasilkan 3.005 contig dan 548 motif perfect SSR dengan frekuensi motif 1/15,27 kb sekuen. AGG trinukleotida merupakan yang paling banyak terdistribusi (37,40%). Sebanyak 380 primer didesain dan 10 primer divalidasi melalui PCR pada 3 klon C. aeruginosa asal Ponorogo, Cikarang, dan Kebumen. Jumlah alel yang terdeteksi sekitar 2 sampai 3 per lokus. Marka mikrosatelit polimorfik yang dihasilkan dari penelitian ini dapat digunakan untuk analisis keragaman genetik C. aeruginosa di Indonesia.
AKTIVITAS ANTIBAKTERI DAN ANTIBIOFILM NANOKOMPOSIT SENG OKSIDA-PERAK (ZnO-Ag) DENGAN MINYAK CENGKEH TERHADAP Pseudomonas aeruginosa Yulianto Ade Prasetya; Khoirun Nisyak; A'yunil Hisbiyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (599.931 KB) | DOI: 10.29122/jbbi.v8i2.4770

Abstract

Pseudomonas aeruginosa is a Gram-negative bacterium that often causes nosocomial infection because of its ability to produce biofilms so that it is resistant to various antibiotics. This research aims to determine the activity of zinc oxide-silver nanocomposites (ZnO-Ag) with clove oil against P. aerugoinosa bacteria. ZnO-Ag nanocomposites were made using the Green One Pot Synthesis technique using a sonicator and microwave instruments. The nanocomposites formed were characterized by X-Ray Diffraction (XRD) to determine crystallinity and particle size and Scanning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX) to determine morphology and elements. The antibacterial activity and antibiofilm tests were carried out using the well diffusion and the microplate techniques, respectively. The resulted ZnO-Ag nanocomposite formed had a size of 19.66 nm, where Ag (47%) was of round shape, while Zn (35%) and O (18%) were fibrous. The ZnO-Ag had an inhibition zone of 14.9 mm against P. aeruginosa and was able to prevent the attachment of the bacterial biofilm for 48 hours with 76,59% inhibition percentage. Pseudomonas aeruginosa merupakan bakteri Gram negatif yang sering menyebabkan infeksi noskomial karena kemampuannya menghasilkan biofilm sehingga resisten terhadap berbagai antibiotik. Penelitian ini bertujuan untuk mengetahui aktivitas nanokomposit seng oksida-perak (ZnO-Ag) dengan minyak cengkeh terhadap P. aeruginosa. Nanokomposit ZnO-Ag dibuat dengan teknik Green One Pot Synthesis menggunakan instrumen sonikator dan gelombang mikro. Nanokomposit yang terbentuk dikarakterisasi menggunakan X-Ray Diffraction (XRD) untuk mengetahui kristalinitas dan ukuran partikel, Scanning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX) untuk mengetahui morfologi dan unsur yang terbentuk. Uji aktivitas antibakteri dilakukan dengan metode difusi sumuran dan uji antibiofilm dilakukan dengan teknik microplate. Hasil nanokomposit ZnO-Ag yang terbentuk memiliki ukuran sebesar 19,66 nm, dimana Ag (47%) berbentuk bulat sedangkan Zn (35%) dan O (18%) berbentuk fiber. Nanokomposit ZnO-Ag memunculkan zona hambat 14,9 mm terhadap P. aeruginosa dan mampu mencegah penempelan biofilm yang dihasilkan bakteri tersebut selama 48 jam dengan penghambatan 76,59%.
UJI AKTIVITAS SITOTOKSIK HERBA KELAKAI (Stenochlaena palustris (Burm.F.) Bedd.) TERHADAP SEL KANKER HATI HEPG2 Masyitah Novia Yanti; Ismi Rahmawati; Wiwin Herdwiani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (642.196 KB) | DOI: 10.29122/jbbi.v8i2.4811

Abstract

Liver cancer is a disease with a high number of cases. Kelakai herb (Stenochlaena palustris (Burm.f.) Bedd.) has cytotoxic activity against MCF-7, MDA-MB-231, DU-145 and HeLa cells. The aim of this study was to determine the cytotoxic activity of the herbal fraction of kelakai against HepG2 liver cancer cells. Kelakai herb was macerated using ethanol 96% followed by fractionation using n-hexane, ethyl acetate, and water as solvent. Cytotoxic test of the kelakai extracts and fractions were undertaken using the 3-[4,5-dimetilthiazol-2yl] 2,5-difeniltetrazolium bromide (MTT) assay method. Immunocytochemical reactions were applied to determine the expression of p53 and caspase-3 proteins in the apoptotic pathway of HepG2 cells. The results showed that the extract, n-hexane, and ethyl acetate fraction of kelakai herbs had cytotoxic activity against HepG2 cells, while the water fraction had no cytotoxic activity. Ethyl acetate fraction as the most active fraction of kelakai herbs could increase the expression of caspase-3 and p53 proteins in HepG2 cells. The ethyl acetate fraction had flavonoids, alkaloids, and tannins which are potent as a cytotoxic against HepG2 cells. Kanker hati merupakan salah satu penyakit dengan jumlah kasus yang cukup tinggi. Herba kelakai (Stenochlaena palustris (Burm.f.) Bedd.) memiliki aktivitas sitotoksik terhadap sel MCF-7, MDA-MB-231, DU-145, dan HeLa. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik dari fraksi herba kelakai terhadap sel kanker hati HepG2. Herba kelakai dimaserasi dengan etanol 96%, dilanjutkan fraksinasi menggunakan pelarut n-heksan, etil asetat, dan air. Uji sitotoksik ekstrak dan fraksi herba kelakai dilakukan menggunakan metode 3-[4,5-dimetilthiazol-2yl] 2,5-difeniltetrazolium bromide (MTT assay). Reaksi imunositokimia digunakan untuk mengetahui ekspresi protein p53 dan caspase-3 pada jalur apoptosis sel HepG2. Hasil penelitian menunjukkan bahwa ekstrak, fraksi n-heksan, dan etil asetat herba kelakai memiliki aktivitas sitotoksik terhadap sel HepG2, sedangkan fraksi air tidak memiliki aktivitas sitotoksik. Fraksi etil asetat sebagai fraksi teraktif dari herba kelakai dapat meningkatkan ekspresi protein caspase-3 dan p53 pada sel HepG2. Fraksi etil asetat memiliki golongan senyawa flavonoid, alkaloid, dan tanin yang memiliki kemampuan sitotoksik terhadap sel HepG2.
EVALUASI PERTUMBUHAN, KANDUNGAN KLOROFIL DAN KAROTENOID TORBANGUN (Coleus amboinicus Lour.) POLIPLOID MELALUI KULTUR IN VITRO Evan Maulana; Darda Efendi; Laela Sari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.6 KB) | DOI: 10.29122/jbbi.v8i2.4834

Abstract

Torbangun (Coleus amboinicus Lour.) is an important medical plant contains an active ingredient. The leaves contain phytochemical which influence to increase of milk production. torbangun was classified as a sterile seed thus this plant cannot be propagated by seed. The polyploid induction is one such approach that may introduce phenotypic characteristic lead to increase genetic diversity. This study aimed to evaluate the growth of torbangun polyploid explants through in vitro which cultured on Murashige and Skoog (MS) medium without PGR. The experiment used a completely randomized design with four replicates. Each replicate consisted of four explants. The parameters observed were shoot height, number of axillary shoots, leaves and roots recorded every week until the 8 weeks of culture. The results showed that torbangun polyploid (tetraploid) had higher growth (shoot height and leaves number) significantly compared to diploid (control) explants, while there were no significantly difference in the number of axillary shoots roots. The polyploid induction of torbangun enhanced the chlorophyll and carotenoids content. Torbangun (Coleus amboinicus Lour) merupakan tanaman obat penting yang mengandung bahan aktif. Daunnya mengandung fitokimia yang berpengaruh terhadap peningkatan produksi susu. Torbangun tergolong benih mandul sehingga tanaman ini tidak dapat diperbanyak dengan biji. Induksi poliploid merupakan salah satu pendekatan yang dapat memperkenalkan sifat fenotipik yang dapat meningkatkan keragaman genetik. Penelitian ini bertujuan untuk mengevaluasi pertumbuhan eksplan torbangun poliploid secara in vitro yang dikultur pada media Murashige dan Skoog (MS) tanpa ZPT. Percobaan menggunakan rancangan acak lengkap dengan empat ulangan. Setiap ulangan terdiri dari empat eksplan. Parameter yang diamati adalah tinggi pucuk, jumlah tunas ketiak, daun dan akar dicatat setiap minggu sampai 8 minggu kultur. Hasil penelitian menunjukkan bahwa poliploid (tetraploid) torbangun memiliki perbedaan pertumbuhan (tinggi tunas dan jumlah daun) yang lebih tinggi dibandingkan dengan eksplan diploid (kontrol), sedangkan jumlah tunas ketiak akar tidak berbeda nyata. Induksi poliploid torbangun meningkatkan kandungan klorofil dan karotenoid.
ANTIBAKTERI EKSTRAK KAPANG ENDOFIT DARI AKAR KAYU JAWA (Lannea coromandelica (Houtt.) Merr.) Saiful Bahri; Puteri Amelia; Normala Rachmawati; Aulia Fitri Firdausya; Firdaus Ramadhan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.107 KB) | DOI: 10.29122/jbbi.v8i2.4855

Abstract

Isolation of endophytic fungi from the roots of Lannea coromandelica could be one way to know the presence of antibacterial activity that serves as a traditional medicinal remedy. This study aims to determine the presence of endophytic fungi at the root of L. coromandelica and their extract potential as an antibacterial agent. Endophytic fungi isolation was performed by direct technique and their macroscopic as well as microscopic characteristics were observed. Screening tests were conducted using methanol and ethyl acetate solvents. Antibacterial tests were conducted on Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis. This study resulted in seven isolates of endophytic fungi (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) originated from the root of L. coromandelica. RLC 5 isolate exhibited the largest diameter of inhibition activity on the screening tests and the largest diameter on the antibacterial tests. The supernatant extract of RLC 5 isolate showed the highest antibacterial activity against the test bacterium S. epidermidis. Isolasi kapang endofit dari akar Lannea coromandelica dapat menjadi salah satu cara untuk mengetahui adanya aktivitas antibakteri yang berfungsi sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui keberadaan kapang endofit pada akar L. coromandelica dan potensinya sebagai agen antibakteri. Isolasi kapang endofit dilakukan dengan teknik langsung lalu diamati karakteristik makroskopik dan mikroskopiknya. Uji penapisan dilakukan menggunakan pelarut metanol dan etil asetat. Uji antibakteri dilakukan terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus epidermidis. Penelitian ini mendapatkan tujuh isolat kapang endofit (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) yang berasal dari akar L. coromandelica. Isolat RLC 5 memperlihatkan diameter daya hambat terbesar pada uji penapisan dan diameter terbesar pada uji aktivitas antibakteri. Ekstrak supernatan isolat RLC 5 menunjukkan aktivitas antibakteri tertinggi terhadap bakteri uji S. epidermidis.
PENGARUH CEKAMAN KEKERINGAN TERHADAP EKSPRESI GEN KETAHANAN OSCATA DAN OSAPX1 PADA PADI TOLERAN KEKERINGAN Fariza Oktaviani; Irma Novita Sari; Tri Handoyo; Tri Agus Siswoyo; Mohammad Ubaidillah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.196 KB) | DOI: 10.29122/jbbi.v8i2.4857

Abstract

Rice is widely cultivated in Indonesia, where one of the problems is drought. Rice plant growth can be inhibited due to a lack of water, which could cause oxidative stress. One mechanism for self-defence involves activating antioxidative genes. This study aims to determine the regulatory response of OsCATA and OsAPX1 resistance genes in rice under drought stress. The rice varieties used were those already pre-tested, including Siak Raya, Sertani 1, Indragiri, IR64. Drought treatments were 0% (control) and 15% PEG 6000. Results indicated that the interaction of rice variety treatment and drought stress had a highly significant effect on the root length and chlorophyll content but no significant effect on the plant height. Also, the gene expression of OsCATA and OsAPX1 increased in rice plants exposed to drought stress. Variety Sertani 1 was recommended due to their high average increase in root length, reduced average plant height and chlorophyll content, as well as increased gene expression in conditions of drought stress. Padi banyak dibudidayakan di Indonesia, di mana salah satu permasalahannya adalah kekeringan. Pertumbuhan tanaman padi dapat terhambat akibat kekurangan air, yang dapat menyebabkan terjadinya stres oksidatif. Salah satu mekanisme pertahanan diri adalah mengaktifkan gen antioksidatif. Penelitian ini bertujuan mengetahui respons regulasi gen ketahanan OsCATA dan OsAPX1 pada padi yang mengalami cekaman kekeringan. Benih varietas padi yang digunakan sudah diuji pendahuluan sebelumnya, antara lain Siak Raya, Sertani 1, Indragiri, IR64. Perlakuan kekeringan yaitu 0% (kontrol) dan 15% PEG 6000. Hasil penelitian menunjukkan interaksi perlakuan varietas padi dengan cekaman kekeringan signifikan terhadap panjang akar, dan total klorofil namun tidak signifikan terhadap tinggi tanaman. Ekspresi gen OsCATA dan OsAPX1 meningkat pada tanaman padi yang mengalami cekaman kekeringan. Tanaman yang direkomendasikan adalah varietas Sertani 1 yang memiliki peningkatan rata-rata panjang akar yang tinggi, penurunan rata-rata tinggi tanaman, dan kandungan klorofil rendah, serta meningkatnya ekspresi gen pada kondisi cekaman kekeringan.
IDENTIFIKASI MOLEKULER JERUK NIPIS TEGAL BERDASARKAN FRAGMEN GEN 18S RIBOSOMAL RNA Yumna Rahmadias Hanifa; Sri Pujiyanto; Rejeki Siti Ferniah; Hermin Pancasakti Kusumaningrum
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (831.43 KB) | DOI: 10.29122/jbbi.v8i2.4883

Abstract

Tegal’s lime is a lime that grows in the coastal areas with high salinity, and research on the molecular identification of Tegal’s lime has never been done before. This study aims to determine the molecular identity and its phylogenetic relationship with other oranges in GenBank. DNA isolation was carried out using CTAB. The best DNA purity was 1.883 while the highest DNA concentration was 464.83 ng/?L. DNA amplification was carried out using the PCR method which consisted of denaturation, annealing, and extension steps. DNA was electrophoresed with agarose gel. The DNA band size was 572 bp. The fraqment of 18S rRNA of Tegal’s lime has a greatest similarity of 99,64% with Citrus sinensis, while based on phylogenetic tree it has a closest relationship with C. aurantium (bootstrap value 33%). The alignment of Tegal’s lime against the C. aurantium sample showed that there were 2 gaps and 4 base changes, while the alignment with other oranges and C. medica as an ancestral showed that there were transition and transversion at the 130th and 560th nucleotide respectively. Jeruk nipis Tegal merupakan jeruk nipis yang tumbuh pada daerah pesisir yang memiliki salinitas cukup tinggi dan penelitian tentang identitas jeruk nipis Tegal masih belum pernah dilakukan sebelumnya. Oleh karena itu, penelitian ini bertujuan untuk mengetahui identitas jeruk nipis Tegal secara molekuler dan mengetahui hubungan kekerabatannya dengan jeruk lainnya pada GenBank. Isolasi DNA dilakukan dengan menggunakan CTAB. Kemurnian DNA terbaik yaitu 1,883 sedangkan konsentrasi DNA tertinggi adalah 464,83 ng/?L. Amplifikasi DNA dilakukan dengan menggunakan metode PCR yang terdiri dari tahap denaturasi, anealing, dan ekstensi. DNA dianalisis menggunakan elektroforesis gel agarosa. Ukuran pita DNA yang didapatkan yaitu 572 pb. Fragmen gen 18S rRNA jeruk nipis Tegal memiliki kesamaan yang paling tinggi dengan Citrus sinensis sebesar 99,64% sedangkan berdasarkan analisis filogenetik, memiliki hubungan kekerabatan yang paling dekat dengan C. aurantium (nilai bootstrap sebesar 33%). Hasil pensejajaran jeruk nipis Tegal terhadap sampel C. aurantium menunjukkan adanya 2 gap dan 4 perubahan basa sedangkan pensejajaran yang dilakukan antara jeruk nipis Tegal dengan jeruk lain dan jeruk nenek moyang C. medica menunjukkan adanya 1 transisi pada basa ke-130 dan 1 transversi pada basa ke-560.
A GLUTARIMIDE FROM THE INDONESIAN MARINE CYANOBACTERIUM Oscillatoria sp. Viqqi Kurnianda; . Khairunnisa; Sofyatuddin Karina; Sri Agustina; Nurfadillah Nurfadillah; Musri Musman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (678.571 KB) | DOI: 10.29122/jbbi.v8i2.4889

Abstract

In continuous concern of marine resources relating to molecular structures that have biological activity around the western part of the Indonesian Archipelago, a marine cyanobacterium species Oscillatoria sp. was found in Sabang Island, Aceh. This study aimed at investigating the chemical and biological contents of Oscillatoria sp. The isolation of the target molecule was carried out based on bioassay-guided separation with several steps of chromatography. In addition, the evaluation of bioactivity was determined by the MTT assay protocol against the human pancreatic cancer Panc-1 cell line. The planar structure of the target molecule was determined by spectroscopic analysis. Results showed that the whole planar structure was identified as cycloheximide-4-acetate (1), a cycloimide class of molecule. Moreover, the relative stereochemistry was confirmed by nuclear overhauser effect (NOE) analysis. Therefore, the molecule was assigned as (2S*,4S*,6S*,7R*)-cycloheximide-4-acetate. The cytotoxicity evaluation of compound 1 showed IC50 value of 0.32 mM against the human pancreatic cancer cell. Dalam perhatian berkelanjutan terhadap sumber daya laut yang berkaitan dengan struktur molekul yang memiliki aktivitas biologis di sekitar bagian barat Kepulauan Indonesia, spesies sianobakteria Oscillatoria sp. ditemukan di Pulau Sabang, Aceh. Penelitian ini bertujuan melakukan investigasi kandungan kimiawi dan biologis dari Oscillatoria sp. Isolasi molekul target dilakukan melalui metode pemisahan berdasarkan uji bioaktivitas dengan menggunakan beberapa tahapan kromatografi. Selain itu, evaluasi bioaktivitas ditentukan oleh protokol uji MTT terhadap sel kanker manusia Panc-1. Struktur planar dari molekul target ditentukan dengan analisis spektroskopi. Hasilnya, struktur planar dari senyawa target diidentifikasi sebagai sikloheksimida-4-asetat (1), kelompok molekul sikloimida. Selanjutnya, relatif stereokimia dikonfirmasi oleh analisis nuclear overhauser effect (NOE). Oleh karena itu, molekul tersebut ditetapkan sebagai (2S*,4S*,6S*,7R*)-sikloheksimida-4-asetat. Evaluasi sitotoksisitas pada senyawa 1 menunjukkan nilai IC50 sebesar 0.32 mM terhadap sel kanker pankreas.

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