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INDONESIA
EUGENIA
Published by Universitas Sam Ratulangi
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The aims of this journal is to provide a venue for academicians, researchers and practitioners for publishing the original research articles or review articles. The scope of the articles published in this journal deal with a broad range of topics, including: Agronomi, Protection, Soil, Forestry, Agroecotecnology.
Arjuna Subject : -
Articles 11 Documents
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Search results for , issue "Vol 17, No 2 (2011)" : 11 Documents clear
APLIKASI METODE ARDRA DALAM IDENTIFIKASI ISOLAT Bacillus thuringiensis ENDOGENIK SEBAGAI PENGENDALI HAMA KUBIS (Crocidolomia binotalis) Salaki, Christina L.; Sembiring, Langkah
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3531

Abstract

ABSTRACT Indonesian indigenous bacterial isolates of B. thuringiensis pathogenic to cabbage pest (C. binotalis) were molecularly characterized and identified using DNA fingerprinting method of ARDRA (Amplified Ribosomal DNA Restriction Analysis). Chromosomal DNA of 10 selected isolates (SLK2.3, SRNG4.2, TKO1, TK9, YPPA1, UG1A, BLPPN8.2, YWKA1, BAU3.2, LPST1) and 2 reference strains (B. thuringiensis serovar kurstaki HD1 & B. thuringiensis serovar israelensis H14) were isolated and purified by standard method. 16S rRNA genes were amplified by PCR method using universal primers of 27f and 1529r. PCR products were digested by 4 restriction endonucleases (EcoR1, HindIII, Pst1 dan HaeIII), and separated by agarose electrophoresis method to generate ARDRA profiles. Results of study showed that only ARDRA profiles generated by Hae III digestion were found to be meaningful and therefore used to identify the isolates. The ARDRA profile analysis indicated that the reference strain of B. thuringiensis serovar kurstaki HD1 could be clearly separated with B. thuringiensis serovar israelensis H14. In fact, those two strains have been widely recognized to be different in terms of their pathogenic specifity against insects. B. thuringiensis serovar kurstaki HD1 has been known to be specifically pathogenic to Lepidopteran whereas B. thuringiensis serovar israelensis H14 has been known to be specifically pathogenic to Dipteran. Key words : application, ARDRA, indigenous, B. thuringiensis, C. binotalis  
PENAMPILAN BEBERAPA VARIETAS TOMAT PADA DUA KONDISI LINGKUNGAN Pongoh, Jantje
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3536

Abstract

Increasing tomato production could be approach by various ways such as intensification and extensification programs. The programs should be supported by availability of seeds and good varieties produced by breeder. Department of Agriculture has released several high yielding varieties.  Tomato grows in a variety of altitude, both the highlands and lowlands, depending on variety. Therefore, ability of adaptation on specific location of tomato variety should be assessed. In relation to that, performance of six tomato varieties was evaluated on two different environments. The experiment was used factorial design. The results showed that performance some varieties varied between the two different environments.  Alista variety and Victory F1 revelead good performance on characters fruit weight and fruit diameter.This two varieties could be used for breeding material. Tomatoes planted on double row space showed better performance then others. Genetic similirity pattern on different row space was resulting different response. Keywords : Performance, variety, environment, similirity
HAMA PENTING TANAMAN UBI JALAR (Ipomea batatas L.(Lamb)) DI KABUPATEN MINAHASA, MINAHASA UTARA, DAN KOTA TOMOHON Pinontoan, Odi R.; Lengkong, Maxi; Makal, Henny V.G.
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3532

Abstract

ABSTRACT The research aimed to identify important pests which attack sweet potato in the field. Field and laboratory research were conducted. The field research was carried out in sweet potato cropping areas in Minahasa Regency, North Minahasa regency and  Tomohon. The laboratory research was conducted in plant pests and disease laboratory, Faculty of Agriculture Sam Ratulangi University Manado to idedntify the important pest species which were found on sweet potato plants. The duration of the study was four months starting from December 2010 until March 2011. Survey method was applied with purposive random sampling.  The research was used 4 period of plant growth namely 1 month, 2 months,3 months, and 4-5 months after planting. The result showed that there were five orders identified, attacking sweet potato since the age of 1-4 months in Minahasa regency, Tomohon and North Minahasa regency. The five orders were Orthoptera, Hemiptera, Homoptera, Lepidoptera, and Coleoptera. The damage on the leaves were caused by pests coreidae, Cicadelidae, Spodoptera sp (Noctuidae), Valanga sp (Acrididae), and Oxya sp. (Acrididae), whereas on the stem was Omphisa sp (Pyralidae). Damage on the tuber was caused by Gryllotalpa sp (Gryllotalpidae), Cylas formicarius (Curculionidae) and Oryctes sp (Dynastidae). Key words : Important Pest Plant Sweet Potatoes, Ipomea batatas L.
KONSENTRASI PACLOBUTRAZOL DAN PEMISKINAN MEDIA PADA PELESTARIAN IN VITRO TANAMAN KRISAN (Chrysanthemum morifolium Ramat) Demmassabu, Sofia; Kojoh, Diane A.; Arsyad, Yulie P.
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3537

Abstract

ABSTRACT   Research was conducted  at biotechnology laboratory of Agriculture Faculty, Samratulangi University Manado since July 2009 until October 2009. The objective was to assess the effect of Paclobutrazol usage and MS media reducing in chrysan perpetuation through in vitro. The research was  designed in randomized complete designed  using factorial. First factor was basic media concentration of Murashige and Skoog with 2 concentration : M1 = 100% MS,  M2 = 10% MS and second factor was paclobutrazol with 4 concentration s which were  P0 = 0 ppm paclobutrazol (control), P1 = 1.0 ppm, P2 = 1.5 ppm, P3= 2.0 ppm,  and P4 = 2.5 ppm paclobutrazol.  The treatments were repeated ten times.  Observed variables were crysan tall, the amount of root and buds that was measured at the end of research. The result showed that 100 % MS media, addition of paclobutrazol obviously produced shorter chrysan, and less amount of roots and buds compare to media without paclobutrazol. Media with 10 % MS combine with   paclobutrazol 2.0 ppm produced shorter chrysan than media without paclobutrazol. The amount of roots and buds in media with paclobutrazol were not different with media  without paclobutrazol. Key words : Paclobutrazol, crysan
PEMANFAATAN BEBERAPA ISOLAT Trichoderma harzianum SEBAGAI AGENSIA PENGENDALI HAYATI PENYAKIT LAYU FUSARIUM PADA BAWANG MERAH IN PLANTA Latifah, Aenul; Kustantinah, .; Soesanto, Loekas
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.4105

Abstract

ABSTRACT The green house research aimed to know the best isolate of T. harzianum in controlling Fusarium wilt , shallot production, and the effect of T. harzianum application time in suppressing the disease. Randomized block design was used with twelve treatments. Each treatment was replicated three times. The treatments were negative control, positive control as wel as extract of ginger isolate, banana isolate, shallot isolate, potato isolate, and ginseng isolates. The treatments were  applied a week before or after inoculation of the pathogen. The result showed that the antagonist extract of ginger isolate applied a week before inoculation decreased the disease intensity by 43.854%. The antagonist extract application applied before or after inoculation were not significant difference . The antagonist of potato isolate extract was the best in increasing number of plants per clump and tuber fresh weight resulting  9.7503 and 13.133 g, respectively. The best extract in increasing root growth was shallot extract. The ginger isolate extract increased root length by 17.672%. Keywords: Trichoderma harzianum isolates, biological control, Fusarium wilt, shallot   ABSTRAK Penelitian rumah kasa ini bertujuan untuk mengetahui isolat T. harzianum yang paling baik dalam mengendalikan penyakit moler dan meningkatkan hasil bawang merah, serta pengaruh waktu pemberian T. harzianum dalam menekan penyakit moler. Rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK), yang terdiri atas 12 perlakuan dengan 3 ulangan. Perlakuan yang dicoba adalah kontrol negatif (tanpa perlakuan T. harzianum dan  Fusarium oxysporum) dan kontrol positif (hanya dengan inokulasi F. oxysporum), serta ekstrak isolat jahe, pisang, bawang merah, kentang, dan ginseng yang diberikan 1 minggu sebelum atau sesudah inokulasi. Hasil penelitian menunjukkan bahwa ekstrak T. harzianum isolat jahe yang disiram sebelum inokulasi Fusarium oxysporum, mampu menurunkan intensitas penyakit sebesar 43,854%. Belum terlihat jelas pengaruh nyata pemberian ekstrak antagonis sebelum maupun sesudah inokulasi patogen. Ekstrak T. harzianum isolat kentang adalah isolat terbaik dalam meningkatkan jumlah anakan per rumpun dan bobot basah umbi, yaitu masing-masing 9,7503 dan 13,133 g, sedangkan ekstrak terbaik dalam pertumbuhan akar yaitu isolat bawang merah yang meningkatkan jumlah akar sebesar 57,452% dan ekstrak isolat jahe menunjukkan pengaruh terbaik meningkatkan panjang akar sebesar 17,672%. Eugenia Volume 17  No. 2  Agustus 2011 Kata Kunci: Isolat Trichoderma harzianum, pengendalian hayati, penyakit layu Fusarium, bawang merah
APLIKASI MAT DALAM PENGENDALIAN LALAT BUAH BACTROCERA SP. (DIPTERA : TEPHRITIDAE) PADA TANAMAN CABE Lengkong, Maxi; Rante, Caroulus S.; Meray, Merlyn
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3533

Abstract

ABSTRACT   Research was aiming to determine the effectiveness of the attractant methyl eugenol + traps modification in the catch of species of fruit flies in the chilli plants at any given time and to identify the types of fruit flies trapped with methyl eugenol + traps modification on chilli crop acreage. The field research was conducted in the area of chilli crop in three places namely District Pineleng, Tompaso / Langowan and Modoinding. Observation number and type of fruit flies was performed at intervals of 2 weeks of observation for 3 months. The result showed that the number of fruit fly caught or trapped dead at 30 traps set in chilli crop acreage was 1278. The highest trapped was 164 whereas the lowest trapped was 89. The number of male fruit flies trapped was high implying that reduction of male fruit fly population in the field. There were five types of fruit flies identified from area of Pineleng, Tompaso and Modoinding namely Bactrocera umbrosa Fabricus, Bactrocera dorsalis (Hendel), Bactrocera philippinensis, Bactrocera carambolae, and Bactrocera sp. The five types were attracted to methyl eugenol. Key words : MAT (male annihilation technique), Bactrocera umbrosa Fabricus, B. dorsalis (Hendel), B. philippinensis, B. carambolae
PERTUMBUHAN DAN HASIL PAPRIKA (Capsicum annuum var-grossum) PADA BEBERAPA JENIS NAUNGAN Tulung, Stella M.T.; Demmassabu, Sofia
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3538

Abstract

ABSTRACT   The objective of this research is to detect the influence of shade kinds to the  growth and yield of paprika and the suitable kinds of shades for paprika. Varieties Yolo Wonder was applied in this research and the research was located at Kakaskasen III, Kecamatan Tomohon Utara for four months.  The research was carried on through Randomized Group Design by using three types of treatments and six replicates.  The treatments were as follows:  without shade, using transparent plastic shade and  using stubble of paddy shade. Detected variable included: the height of the plant, leaf area, amount of the fruit, length, diametre and the weight of the fruit. The height of the plant measured every week since two weeks after planting  and the leaf area was measured according to the height of the plant. The amount, lenght, diametre and the weight of the fruit measured during the harvest time. The result of the research shows that diffrerent kinds of shades brings different influence to the growth and yield of paprika and the research shows that the suitable shade for the growth and yield of paprika is transparent plastic shade. Keyword: paprika, shade
KEANEKARAGAMAN JENIS DAN KELIMPAHAN POPULASI SERANGGA AIR SEBAGAI INDIKATOR BIOLOGIS CEMARAN AIR PADA DAS DI LANGOWAN Maramis, Redsway T.D.; Makal, Henny V.G.
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3529

Abstract

ABSTRACT   Aquatic insects are one of the organisms that are used in determining water contamination. Aquatic insects are sensitive to the presence of waste dumped in the river and some are resistant to such waste. One of insect resistant waste is Chironomidae. The research objective was to determine aquatic insect species, population abundance by calculating indices diversity and biotic index. Aquatic insects in the lake watershed Tondano were collected by kicks and Hand Picking. Result showed that seven orders, theerteen families and sixteen species had been identified. The number of highest aquatic insect was found in Noogan which were 13 species, whereas in karondoran were 9 species. Aquatic insects were identified including, Heptagenia sp, sp Baetis sp, Caenis sp., Hydropsyhe, sp and Chrironomidae. The highest aquatic insect populations were collected in Karondoran followed by Winebetan and Noogan. The highest diversity index of aquatic insects was found in Winebetan whereas the lowest was in Karondoran. The lowest value of the biotic index was identified in the region Noogan suggested that water in Noogan area was clean water compared to Winebetan and Karondoran. Key word: Types and aquatic insect populations
BIOLOGI DAN DEMOGRAFI TUNGAU MERAH Tetranychus spp. (Acari: Tetranychidae) PADA TANAMAN KEDELAI Mamahit, Juliet M.E.
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3534

Abstract

ABSTRACT   Red spider mite Tetranychus spp. are pest on several ornament and food plant. The objectives of this research were to study biology and demography Tetranychus spp. on soybean in condition laboratory. A piece of soybean leaf measures 3 cm2 at place above cotton in petridis. Above the leaf were put a pair red spider mite to watch the biology of mite.  The research of demography of red spider mite was done observation in one hundred egg that used to come from age class same (cohort). The observation was done every day until the egg become to adult. The result showed that the mean of fecundity of red spider mite were 54.16 egg. The total life time of female 13.28 day and sex ratio 1:1.5. The intrinsic rate of increase the population were 0.253 individual per day, the discrete daily growth rate were, 1.28 individual/day, the net reproduction rate were 25.515 individual/female/generation. The generation time were 13.567 days. The stable population of Tetranychus spp. in laboratory were 67.2% egg, 24.6 nymph and 8.2% adult. The intrinsic birth and death rate were 0.3 and 0.05 individual/female/day. Key words: Tetranychus spp., Demography, Soybean
CONTROL OF CACAO POD BORER, CONOPOMORPHA CRAMERELLA SNELLEN ON CACAO PLANTATIONS IN NORTH SULAWESI USING DEGRADABLE POLYMER SLEEVES Sembel, Dantje T.; Watung, J.; Shepard, Merle; Hamming, Mike; Carner, Garry R.
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3530

Abstract

ABSTRACT Research was aiming to control the cocoa pod borer, Conophomorpha cramerella using different thickness of degradable plastic and regular plastic by sleeving to protect pods. The studies were carried out in abandoned cacao plantation at Sapa, and commercial cacao plantations at Pungkol in the sub-district of South Minahasa in North Sulawesi, Indonesia. Result showed  that sleeving with plastic at different thickness can reduce markedly the infestation by CPB but there was no significant difference between thickness and type of plastic. Between 85 to 95 % of the pods sleeved with degradable and  non- degradable plastic at the commercial cacao plantation  were not damaged by CPB  but 96 % of control (unsleeved pods)  was damaged by CPB. Key words: Conopomorpha cramerella, cocoa plantations, degradable plastic bags

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