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BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan)
Published by Universitas Medan Area
ISSN : 25975269     EISSN : 2356458X     DOI : 10.31289
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biolink focuses on the publication of the results of scientific research related to fields. This article is published in the internal and external academic community of the University of Medan Area (UMA) especially in the field of Biology. Biolink publishes scientific articles in the scope of biology that includes environmental biology, industrial biology and health biology. Published articles are the results of research articles, studies or critical and comprehensive scientific studies on important issues related to the field of biology.
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Articles 11 Documents
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Search results for , issue "Vol 8, No 1 (2021): August 2021" : 11 Documents clear
DEGRADATION OF SOME POLYSTRENE USING BEETLE LARVAE (TENEBRIO MOLITOR L.) Ichsan Luqmana Indra Putra; Alfi Nurcahyasari
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.5001

Abstract

One alternative to waste processing polystyrene is to use Tenebrio molitor larvae. This study aims to determine the increase in body length and weight, rate of degradation, and value of Waste Reduction Index (WRI) of larvae T. molitor. This study used laboratory experimental using completely randomized design experimental design model (CRD) data analysis with control and 4 feed treatments. Each treatment level was repeated 5 times in order to obtain 25 experimental units. Larvae were reared in a plastic container containing 10 larvae. Each larvae were given bran feed for control and polystrene for treatment, each feed as much as 1 gram. The polystyrene used were food packaging, walls, electronic packaging, and ice boxes. Larvae were measured for length and body weight every 3 days for 30 days. Parameters observed were increase in length and weight of larvae, rate of degradation and WRI values.The highest mean value of increase in larval length on the polystrene wall was 1.69 cm. The lowest value was polystyrene food packaging (1.59 cm). The average weight gain of larvae has a uniform value in the control and treatment of 0.07 gr. The highest degradation rate and WRI values were polystyrene wall and electronic packaging at 0.019 and 0.63%. while the lowest values were polystyrene food packaging at 0.11 and 0.37%.
LARVICIDAL ACTIVITY OF TURMERIC (CURCUMA DOMESTICA) EXTRACT AGAINST AEDES AEGYPTI L. Indah Tri Susilowati; Tri Harningsih; Indra Ayu Vidyaningrum
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4353

Abstract

Indonesia occupies the top position as the country with the highest dengue cases in ASEAN. A variety of prevention dengue can be done which can be used chemicals. Chemical insecticides indeed provide effective results and optimal, but many negative impacts both on the environment and living organisms. The many negative effects of   chemical insecticides led to new research in the vector control safer, simpler, and environmentally sound. Control using biological insecticides (vegetable) is one of them. One of the plants that can be used as larvicides are turmeric (Curcuma domestica). Tumeric contains bioactive compounds such as essential oils, alkaloids, flavonoids, saponins potential as an alternative killer mosquito larvae. Researchers create 6 concentration turmeric extract (0g / L; 0.25 g / L, 0.5 g / L; 1g / L; 2g / L; 4g / L) of each concentration included 25 larval Aedes aegypti L. The data obtained were performed Shapiro-Wilk normality test and then test the hypothesis by Kruskal Walis and probit test. Results obtained LC50value is 2.084g/L or 0.208% (w/v). The results showed that tumeric extract (Curcuma domestica) is effective to kill the larvae Aedes aegypti L.
THE STUDY OF TREE LEVEL IN ANGGOMATE WATERSHED AREA , SOUTHEAST SULAWESI Ikbal Ikbal; Aqmal Khaery; Fachruddin Fachruddin
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4581

Abstract

Anggomate River has ecological and economic value for the local community. Massive community activities in utilizing land in the Anggomate watershed area for plantation, inhabitation, and sand mining lands can cause the tree vegetation decline, even flood often occurs in Andowia region annually. The research method used was a quantitative method. Sampling was performed through a plot-transect method. The plots were placed on the downstream (Labungga Village river area), middle (Laronaga Village river area), and upstream (Andowia Village river area) regions. The study results showed that there are 35 tree species with the individual number of 64. The highest importance value index was obtained from Ficus benjamina L. at 29.38%, Anthocephalus cadamba (Roxb.) Miq) at 25.64%, and  Lansium domesticum Corr. at 16.81%. The dominance index of the tree species in the Anggomate river area was 0.971, which indicates that one of the tree species dominated this area, namely, Anthocephalus cadamba (Roxb.) Miq) (Roxb.) Miq). The diversity index in the Anggomate watershed area includes in high category (H’= 3.402 atau H’ > 3). The distribution index includes in the evenly distribution category due to obtaining closely approaching the value of 1 (E= 0.818).
EVALUATION ON METHODS FOR IDENTIFICATION OF VIBRIO SP.: KAWAKAWA (EUTHYNNUS AFFINIS) BRINING SHREDDED AND CHITOSAN ADDITION AS PRESERVATIVE Purwaningtyas Kusumaningsih
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.5011

Abstract

The kawakawa (Euthynnus affinis) brine salting that used to make shredded were preservative with salt. Salt addition has aim to inactivate of bacterial contamination. Obviously, bacteria is still capable of growing in kawakawa brine shredding Therefore, in this study chitosan was added as antibacterial in shredded processing. Vibrio sp., is one of common halophilic bacteria found in seafood. If this bacteria is consumed, it can cause serious problems in human health. The objective of this study was to evaluate the factors that affect in identifying Vibrio sp., on kawakawa brine shredding (C), shredded non-chitosan (FC) and shredded contain of chitosan (FC+). The methods evaluated were steps in enriching bacteria, culturing bacterial in selective media and analysing bacterial by API 20E kit. Enriching and incubation periods were needed by halophilic bacteria to adaptation in new environment. It was required to observe the bacteria characteristics' that would be isolated. Bacterial colonies were growth on Thiosulphate citrate bile salt sucrose (TCBS)  were not Vibrio sp., but confirmed as Pseudomonas luteola and Proteus vulgaris based on API 20E analysis. It was showed that TCBS media had some advantages in identifying Vibrio sp. In conclusion, to get the best result in identifying bacteria, at least two  or more methods were used to avoid misidentification.
ISOLATION AND CHARACTERIZATION OF IAA-PRODUCING RIZOSPHERE BACTERIA FROM RICE PLANTS (ORYZA SATIVA L.) IN KEDUNGPANI URBAN VILLAGE, SEMARANG CITY Sutrisno Sutrisno
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4556

Abstract

Rhizosphere bacteria live in the soil around plant roots. Various rhizosphere bacteria are able to produce the hormone Indole-3-Acetic Acid (IAA) which stimulates plant growth. This study aims to isolate and characterize IAA-producing rhizosphere bacteria in rice plants in the rice fields of Kedungpani urban village, Semarang city. Bacterial enumeration was carried out using the total plate count (TPC) method. The isolates obtained were characterized by observation of colony and cell morphology, gram staining, endospore staining, catalase test, and sugar fermentation test. The ability to produce the IAA hormone was tested using Salkowski's reagent. The enumeration results showed that the total bacteria in the sample was 2.9 x 105 CFU / g. A total of 10 isolates were obtained and were able to produce the IAA hormone with various concentrations. Isolates KP1, KP2, KP3, KP4, KP5, KP6, KP9, KP13, KP14, and KP15 produced IAA with concentrations of 3.389, 5.111, 3.000, 1.667, 1.944, 5.056, 5.444, 4.500, 5.056, and 10.833 ppm, respectively. The results showed that there were 5 isolates with the highest IAA concentrations, namely KP2, KP6, KP9, KP14, and KP15 which had the potential to be used as biostimulant agents for plants.
DISTRIBUTION PATTERN OF THE EXOTIC PLANT (SYNEDRELLA NODIFLORA (L) GAERTN) IN THE REHABILITATION ZONE, RESORT OF WONOASRI, MERU BETIRI NATIONAL PARK Yennita Dwi April Liana; Hari Sulistiyowati; Arif Mohammad Siddiq
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4876

Abstract

Exotic plants are plants that are intentionally or unintentionally included in an area. Based on the results of the 2020 survey, one of the exotic plants found in the Donglo Block Rehabilitation Zone area of Meru Betiri National Park (MBNP) is cinderella weed (Synedrella nodiflora). The objective of this study is to determine the distribution pattern and area of cover of exotic S. nodiflora plants in the research location. Data were collected using a combination method of systematic plot transects measuring 2 x 2 meters which were placed along the transect with a total of 415 plots. The data recorded were the coordinate position of each individual S. nodiflora and the area of the cover. Analysis of distribution pattern data using the Morisita index and visualized with a spatial distribution map using Geographic Information System (GIS). Coverage area analysis was carried out by calculating the percent cover of S. nodiflora. The results of the analysis of the Morisita index showed the value of Iδ = 7.13, which indicates that the distribution pattern of the plants is clustered. In addition, the presence of this species in the study area was occupied 41.7% or 0.85 ha of the total area of 2.04 ha. It shows that this species still did not dominate the area, so it can be said that this species has not yet become an invasive species category in the research location.
AN OVERVIEW OF BRYOPHYTES DIVISION Nurmaini Ginting; Hanifah Mutia Z.N Amrul; Ferdinand Susilo
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4294

Abstract

Bryophyte is a non-vascular plant, consisting of three classes: mosses, hornworts, and liverworts. The body forms have a thallus, and some have a leaf-like organ. The class division of the Bryophyte division is base on anatomical and morphological traits, such as meristem tissues, stomata, and vascular tissues. M Bryophyte has no vascular tissue, but it has a capillary that has functions like xylem and commonly refers to as hydroid. Mosses and hornworts have stomata on the sporophyte part, while in the liverworts, stomata are not found. Hornworts have a basal meristem that has an unpredictable cleavage. Meristem on the mosses only divides up the formation of sporangial. In the liverworts, the apical meristem does not see. Members of the hornworts consider the most advanced because the meristematic phase is more prolonged, so that it is closer to the characteristics of Tracheophyta group.
ANALYSIS OF DNA SEQUENCE ENCODING GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPDH) ON COCOR BEBEK (KALANCHOE x LAETIVIRENS) Putri Agustina; Dewi Indriyani Roslim
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4964

Abstract

To understand the fundamental mechanisms on genes in plant cells can be performed by gene expression studies. The study needs reference genes that act as internal control to avoid expression data bias. Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) gene is one of reference genes that has been validated in plants. This study aims to analyze the DNA sequences encoding GAPDH in cocor bebek (Kalanchoe x laetivirens). Methods included total DNA extraction from fresh leaf, polymerase chain reaction (PCR), electrophoresis at 1% agarose and DNA sequence data analysis. The DNA sequence of K. x laetivirens GAPDH gene obtained were 1107 bp and predicted to consist of 5 exons and 5 introns. This sequence had about 68.96%-73.81% similarity to the DNA sequence of the GAPDH gene in several plant species. The amino acid sequence of K. x laetivirens GAPDH had 5 conserved regions and residues of cysteine (C) and histidine. The sequence obtained in this study was the first to be reported from the genus Kalanchoe. This sequence can be used for analysis of gene expression in K. x laetivirens and can be as reference for isolating the GAPDH gene for other plant species in the genus Kalanchoe.
INCREASED RESISTANCE OF PENAEUS MONODON TO WHISPOVIRUS CAUSES WHITE SPOT SYNDROME THROUGH ANTIVIRUS GENE TRANSFER Chelvieana A. Christanti; Debora V. Widyanti; Yesica C. Rhenata; Floreta D. Icasari; David L Gultom; Sudarsono Siburian; Wahyu Irawati
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4597

Abstract

The shrimp species Penaeus monodon is the main commodity of Indonesian aquaculture. One of the obstacles when cultivating tiger prawns is the attack of white spot disease (White Spot Syndrome Virus) which makes the quality and quantity of production decrease. One attempteffective for overcoming the white spot virus attack on shrimp, namely through the transfer of antiviral genes. Previous researchers carried out the transfer of antiviral genes to provide immunological effects so that they were resistant to pathogen attack and could prevent the death of tiger prawns. Destinationwriting article that is This study aims to determine the increase in resistance of tiger shrimp to Whispovirus that causes White Spot Syndrome through the transfer of antiviral genes. The type of research conducted is descriptive qualitative with a literature review method that discusses five focus studies, namely Whispovirus as a cause of White Spot Syndrome Virus (WSSV) disease in tiger prawns, tiger shrimp infected with White Spot Syndrome Virus, the use of PmAV antiviral genes in the treatment of Whispovirus , the mechanism of transfection of antiviral genes in the treatment of Whispovirus infection against tiger prawns, and changes in tiger shrimp resistance after insertion of antiviral genes. The conclusion obtained is that the addition of antiviral genes in tiger shrimp DNA can increase its resistance to Whispovirus.
ANTIBIOTIC SUSCEPTIBILITY TEST TOWARDS COCKROACH (PERIPLANETA AMERICANA) FOOT BACTERIA IN THE NUTRITION DEPARTMENT AREA OF PALU CITY HOSPITAL I Nengah Nengah Kundera; Yulia Windarsih; Abd. Rauf
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4945

Abstract

Cockroaches (Periplaneta americana) are known to have potential as vectors of various pathogenic bacteria in various locations including the nutrition department area in the hospitals. Research related to the role of these animals as vectors of pathogenic bacteria has not been done. The provision of food in the hospital nutrition department has the potential for bacterial contamination from food, equipment, hospital staff, and the environment. Cockroaches can be a vector for spreading various nosocomial infectious diseases in hospitals. A descriptive method was used, and the data were obtained by bacterial isolation, Gram staining method, antibiotic sensitivity test, and bacterial identification using Microbact system software 2000. This study was carried out at the Biology Education Laboratory, Education and Teacher Training Faculty,  Tadulako University. Based on the results of the study, 6 species of bacterial isolates from cockroach feet   in the nutrition department area of Palu city hospital, namely E. coli, S. arizonae, Salmonella sp., K. ozaenae, S. simulans, and S. enterica. Antibiotic sensitivity test showed that four bacteria exhibited multi-drug resistance to antibiotics Amoxycillin, Chloramphenicol and Vancomycin. Meanwhile,S. enterica was still sensitive to the five tested antibiotics namely Amoxycillin, Chloramphenicol, Ciprofloxacin, Ofloxacin, and Vancomycin. serious policies and handling are needed to control new types of vectors that carry diseases in humans.. meanwhile,S. entericawas still sensitive to the five tested antibiotics.

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