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INDONESIA
BIOEDUKASI
Published by Universitas Jember
ISSN : 16933931     EISSN : 25800094     DOI : -
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Library & Information Science
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 17 No 1 (2019)" : 8 Documents clear
Bioinformatics Analysis to Construct Cellulose-binding Module Synthetic Gene and Design Primer Febriana Dwi Wahyuni; Asrul Muhamad Fuad
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13204

Abstract

Cellulose-binding module (CBM) is a protein domain commonly found in various types of cellulase enzymes. The function of this CBM can be used for the binding process and the immobilization of a protein in the cellulose matrix. CBM can be obtained from several organisms, one of them is Trichoderma reesei. To get a gene, it does not have to be isolated from the original organism. Gene sequences can be obtained synthetically through bioinformatics analysis in accordance with the same gene sequences as those at Gene Bank. Bioinformatics analysis can be used to find new gene sequences or existing genes. This study aims to get a cbmsyn synthetic gene quickly and efficiently without reducing protein activity, which can then be ligated with other genes so that it functions as an immobilized enzyme. From the results of bioinformatics analysis, obtained DNA sequences measuring around 498 pb with 166 amino acid protein lengths. The sequence was modified by adding several restriction sites, namely BamHI, AfeI, and ScaI. The DNA sequences obtained were optimized with the Pichia pastoris codon.
The Effect of Cocoa Leaf Extract (Dendrophthoe pentandra (L.) Miq.) on The Growth of Shigella dysenteriae and Salmonella typhi Della Mahda Yunita; Joko Waluyo; Siti Murdiyah
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13205

Abstract

Cocoa parasite leaves (Dendrophthoe pentandra (L.) Miq) belong to parasitic plants against hosts which are potentially used as traditional medicinal ingredients. However, the efficacy of cocoa parasite leaves is still not widely known by the public. One of the properties from cacao parasitic leaves is able to treat mild diseases such as smallpox, hookworm, flu, cough and diarrhea as well as being able to be used as a treatment for serious diseases such as tumors and cancer. The bacteria causing infection generally come from gram negative bacteria, namely Shigella dysenteriae and Salmonella typhi which refer to enteropathogenic bacteria causing infection to the host. The existing controls still use antibiotic drugs that are used less procedurally and causing bacteria more resistant. Cocoa parasite leaf extract contains active compounds in the form of alkaloids and saponins which play a role in inhibiting bacterial growth. This study aims to determine the effective concentration on the effect of cocoa parasite leaf extract to the growth of Shigella dysenteriae and Salmonella typhi bacteria. The research method was by using diffusion so that by using wells with 5 repetitions. The concentration used was 8%, 10%, 12%, 14%, 16%. Data were analyzed by using ANOVA analysis to determine the effective concentration on the effect of extracts to the inhibition of bacterial growth. The significant values were indicated at 0,000 against Shigella dysenteriae and 0.042 bacteria against Salmonella typhi (<0,05). The data revealed that cocoa parasite leaf extract has the potential to inhibit the growth of Shigella dysenteriae and Salmonella typhi bacteria.
Isolation and Antibacterial Activities of Actinomycetes from Rhizosphere Plant Cane (Saccharum officinarum) on Escherichia coli and Staphylococcus aureus Wahyu Nur Sulistyanto; Guntur Trimulyono
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13430

Abstract

The phenomenon of increasing human bacterial infections and increased resistance to antibiotics encourages the exploration of more antibiotic producers. One source of antibiotics comes from the Actinomycetes bacteria, the source of isolation comes from the rhizosphere of sugarcane plants because it is known in the rhizosphere to produce exudates that are beneficial to bacteria. The purpose of this study was to obtain Actinomycetes isolates and determine the antibacterial abilities of selected isolates. Initial isolation was carried out using Starch Casein Agar (ScA) media, then color grouping, Gram test and cell morphology observation. The next stage is testing the antibacterial activity carried out using the well method. Data obtained in the form of basic characters Actinomycetes, inhibition zone diameter and antibacterial activity count. The results showed that 4 isolates had antibacterial ability against test bacteria, among others, ACB34a isolates, ACB44c isolates, ACB44c isolates and ACB55c isolates. Based on the results of measurement and calculation of antibacterial activity against E. coli, it can be known in succession, among others, ACB44c isolates (9.62 mm2 / 10µl) and ACB54c (0.38 mm2 / 10µl), then antibacterial activity against S. aureus. among others, ACB44c (27.33 mm2 / 10µl), ACB54c (26.41 mm2 / 10µl), ACB55c (8.04 mm2 / 10µl), and ACB34a (8.04 mm2 / 10µl). Keywords: Actinomycetes; Antibacterial; Characterization; Rizosphere of sugar cane
The Development of Analytic Team Collaborative Learning Model Based on Brain-Based Learning (BBL) for Junior High School Science Learning in Agroecosystem Areas Yolanda Leony Agustia Mertha; Imam Mudakir; Jekti Prihatin
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13190

Abstract

Abstract Students in the agroecosystem area are generally less active because the teacher does not utilize the potential of the surrounding agroecosystem. This can be overcome by applying Brain-Based Learning (BBL) approach combined with AT collaborative learning model. This study aimed to produce Analytic Team learning model based on Brain-Based Leaning (AT-BBL) that is valid, practical, and effective, and also improves the students' critical thinking skills and learning outcomes. This study used R&D with 4D development model which only carried out three stages, namely define, design, and development without using the disseminate stage. The average validation is 86.98 (highly valid). The average percentage of teacher responses is 92.94%, and the average percentage of student responses is 91.22% which is very practical. Keywords: Agroecosystem, BBL approach, Analytic Team Learning Model, Collaborative Learning
Effectiveness Application of Local Wisdom Ecological Modules Kasepuhan Cisungsang Community Through Utilization of Ecosystems on Student Learning Outcomes of 10th Class in Senior High School Aditya Rahman; Nurul Anisa; Bambang Ekanara
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13191

Abstract

This study aimed to analyze the implementation of learning module of ecology based on local wisdom of society in Kasepuhan Cisungsang. This research and development study was conducted in small scale involving 30 students on SMAN 1 Petir, Serang, Banten. Simple random sampling was used for sampling methods. Instruments for data collections were using objective test, reflection journal, observation sheet and questionnaire. The effectivity of module was rated based on student learning completeness (grade > 65). The result showed 83% of students have completed in cognitive assessment, 87% of students have completed in psychomotor assessment. Questionnaire for student response showed the average value of 43% in very good category and 57% in good category. It can be concluded that the learning modules effective to facilitate student learning outcomes.
The Effect of Problem Based Learning to Critical Thinking Ability of M1 Students in Plant Biotechnology Materials Iir Nur Choiriya; Erlia Narulita; Areerat Chuseng
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13200

Abstract

This study aimed to determine the effect of learning based on learning problems on students' critical thinking skills in plant biotechnology materials. This research is a quasi-experimental type using the design of "Post test and pretest, Non Equivalent Control Group Design" which consists of two experimental classes. The first experimental class used the Problem Based Learning learning model and the second class used a discussion learning model. Problem-based learning motivates students to learn independently to find information themselves from various sources, such as the environment, media and the internet. This learning trains students to think critically about contextual problems related to the material so as to improve students' cognitive abilities ranging from identifying problems, formulating hypotheses, analyzing problems, evaluating and concluding.
Impelementation of Problem Based Learning with Electronic Media on Student Learning Outcomes in Indonesia's - Philippines International Class Nuris Fattahillah; Slamet Hariyadi
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13202

Abstract

The curriculum in Philippines different with the curriculum in Indonesia. This make the learning process and the problems faced by students in Indonesia and Philippines different. One of the problems experienced by students is caused by the lack of variation in strategies in the teaching and learning process. The instructional model used by the teacher is to upgrade student learning outcomes, especially on high-level cognitive, in this research especially problem solving abilities. Instructional method used by researchers is Problem Based Learning (PBL) with a pretest posttest design. Data of this research obtained quantitatively and using the mean before and after the test. This study used control class and experimental class on material cells to organism. The results show that the experimental class has a higher average value of 88.6 compared to the control class, 81.9. These results indicate that PBL successfully increases learning activities and students can follow the learning model using PBL.
The Effect of Giving Physical Punishments to Student Awareness of As-Shakireen School, Waeng, Narathiwat, South Thailand Ria Yulian; Slamet Hariyadi; Nuainee Awaesoh
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13203

Abstract

One of the requirements for the process of conducting education can take place effectively, namely the enforcement of student discipline both in attendance and participation in the education process, namely the application of punishment. The aim of this study is to determine the impact of punishment on students, the method is descriptive method, because the descriptive method intends to solve the problem of describing something state / subject based objects on what it is. The form of research is an interrelationship study, namely by conducting data collection. The result of this study is percentage that has a comparative analysis of mild and severe penalties in the form of a ratio. Respondents clearly stated that physical punishment had an effect on students' academic / career achievements (65%) while mild physical punishment had an adverse negative impact (35%). Looking further at the effects of physical punishment, it was observed that severe and severe penalties prevented class participation, reduced attendance and increased the dropout rate shown by respondents as wise. According to the explanation above, it can be concluded that the application of penalties, especially physical punishment to students will make students feel depressed and afraid that will have an impact on student psychology.

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