cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
,
INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue "Vol 19, No 2 (2024): August 2024" : 9 Documents clear
Bioflocculant Technology Implementation Using Navicula sp for Harvesting of Arthrospira Platensis Culture Suyono, Eko Agus; Satriayudistira, Jody Ashrib; Baihaqi, Yahya; Amelia, Ria
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.883

Abstract

Bioflocculation, a microalgae harvesting method utilizing organisms likebacteria and microalgae as flocculant agents, necessitates careful selectionand ratio determination of bioflocculant agents. This study seeks to ascertainthe optimal balance of bioflocculant, Navicula sp., for metabolite productionand harvesting efficiency in Arthrospira platensis culture. Spanning three oneweek cultivation stages, the research commences with A. platensis cultureunder varying salinities (0, 15, 25, and 35 ppt) in the first stage, followed bypH variations (9, 10, 11, and 12) in the second stage. The optimal salinity-pHcombination identified in the first two stages is applied in the third stage,involving bioflocculant addition to culture and medium solutions at varyingratios (0.25:1, 0.5:1, 0.75:1, and 1:1 v/v). Precipitation efficiency andpigmentation were measured using spectrophotometry, while lipid contentwas determined by the Bligh Dyer extraction method. Carbohydrate contentwas assessed using the Phenol-Sulphuric Acid assay, and protein contentwas quantified using the Bradford protein assay. The findings indicate salinity,pH, and bioflocculant influence A. platensis growth, metabolite production,and harvesting efficiency. Optimal outcomes are achieved at 15 ppt salinity,pH 9, and a 1:1 ratio of bioflocculant, yielding 1.18x10-3 g of dry biomass,3.56x105 cells/mL of cell density, 2.9x100 g/L of carbohydrates, 3.63x100 g/Lof lipids, 9.97x10-3 mg/L of chlorophyll a+b, 8.71x10-1 mg/L of carotenoids,and 3.04x10-1 g/L of protein. These results suggest that salinity addition, highpH, and the addition of Navicula sp. as bioflocculant increase the harvestingprocess efficiency of A. platensis culture.
Preface Vol 19 no 2 Agustus 2024 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.1034

Abstract

Cover Vol 19 no 2 Agustus 2024 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.1035

Abstract

Microplastic Occurrence in Different Fish Organs from Two Coastal Waters in Java Sea, Indonesia Dwiyitno, Dwiyitno; Barokah, Giri Rohmad; Hidayah, Izhamil; Januar, Hedi Indra; Wibowo, Singgih
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.941

Abstract

Plastic debris and microplastic (MP) have been associated with marinepollution. The present study aimed to assess the MP accumulation in differentorgans (gut and gill) of 6 economically important fish species from twoessential coastal areas, Jakarta Bay and Cirebon Bay. MP was isolated withNaCl, followed by the identification of MP profiles with digital microscope andFourier Transform Infrared Spectroscopy (ATR-FTIR), respectively. The resultshowed that MP was found in all samples. Generally, MP concentration in fishfrom Cirebon Bay was slightly higher than that from Jakarta Bay, with benthicspecies more concentrated than pelagic fish in both sites. Microplastic wasmore concentrated on the gill of fish from Jakarta Bay, in contrast withCirebon Bay. The predominantly MP in Jakarta Bay fish was fiber, while in CirebonBay was fragmented with minor granule and film in both locations. Polyethylene(PE), polyvinyl chloride (PVC), and polypropylene (PP) were the most dominantMP in the fish samples from both study areas. Microplastic size showed thatthe majority (65%) was the small size (1 mm), compared to the biggerone (1-5 mm), while blue, black, and red are the predominantly MP colors.This research supports the need to minimize plastic pollution in aquaticecosystems to tackle the detrimental impact of MP accumulation to fish andhuman health. It is suggested to compare MP profiles on fish samples and inwater or sediment compartments and identify the chemical constituents ofMP.
Optimization of Catfish (Pangasius sp) Bone Bio-calcium Production with Different Concentrations of Citric Acid and Stirring Time Using the Response Surface Method (RSM) Approach Wijayanti, Ph.D, Ima; Agustini, Tri Winarni; Swastawati, Fronthea; Anggo, Apri Dwi; Afifah, Diana Nur
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.867

Abstract

Calcium is an important macro-mineral that is really needed by the body.Calcium from fish bones can be an alternative source of calcium for thosewho are allergic to milk lactose which is usually used as calcium source. Theweakness of fish bone calcium is that it still has a fishy odor, which reducesthe hedonic value of products fortified with fish bone calcium. This researchaims to determine the optimization of concentration and stirring time in citricacid to produce bio-calcium from catfish (Pangasius sp) bones with the bestcalcium content and hedonic characteristics using Response SurfaceMethodology (RSM). The citric acid concentration tested was a minimum of0.25% and a maximum of 1% with stirring times of 30, 60 and 90 min. Dataprocessing was carried out with the help of Design Expert 11 software. Therecommended model is the quadratic model. The recommended optimizationresults for the bio-calcium production solution are a citric acid concentrationof 0.25% and a soaking time of 30 min. Verification was carried out accordingto the optimization solution and gave yield results of 88.9%, particle size of51.07 µm, calcium content 24.05; with a color hedonic value of 4.115, aroma4.059 and overall 4.090 out of a maximum value of 5. XRD pattern showed nodifference between fishbone powder and bio-calcium with degree crystallinity57 and 56.2%, respectively. FTIR spectra showed that both fishbone powder andbio-calcium powder contained hydroxyapatite, however citric acid slightly removedprotein due to absence of amide 1 peaks in bio-calcium powder. Citric acid at0.25% for 30 min helped to increase the yield and reduced some organiccompound to obtain bio-calcium powder. The proper concentration of citric acidand stirring time yielded catfish bone bio-calcium with higher calcium contentand hedonic value.  
The demineralization of Sardinella gibbosa scales using hydrochloric acid and high-pressure carbon dioxide Saleah, Faisa; Netramai, Siriyupa; Kijchavengkul, Thitisilp; Wipatanawin, Angkana; Rosales, Czarina Kristine
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.896

Abstract

This research aimed to investigate and compare the demineralization ofgoldstripe sardinella (GS; Sardinella gibbosa) scales, a major by-product inthe canned fish industry prevalent in East Africa and Southeast Asia, particularlyThailand. The study focused on conventional and alternative demineralizationmethods, assessing the yield and quality of the demineralized scales. Afterremoving minerals, fish scales have potential value as an alternative sourceof collagen and gelatin. For the strong acid treatment using hydrochloricacid (HCl), the effects of HCl concentrations (0.2-1.5 M) and treatmenttimes (30-120 min) on demineralization efficiency were assessed. The resultsfrom RSM indicated that HCl concentration was the only treatment factorthat significantly affected demineralization yield, HCl concentrations at orabove 0.82 M gave a demineralization efficiency of e” 99%, independent oftreatment time (P 0.0001). A preliminary investigation into high-pressurecarbon dioxide (HPCD) treatment of GS scales (at 10 bar for 1 to 4 h)showed comparatively lower demineralization efficiency. Within the studiedparameters, the highest demineralization efficiencies for both methods were99.89±0.06 and 16.13±1.92%, respectively. SEM images and EDX analysisconfirmed the complete removal of minerals (primarily Ca and P) after HCltreatment using HCl 0.85 M for 30 min. Conversely, HPCD-treated scalesexhibited altered structure and physical damage.
Characterization and Potential Utilizations of A Marine Euglenophyte from Okinawa, Japan Prabowo, Danang Ambar; Suda, Shoichiro
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.895

Abstract

mong many microalgae species, certain euglenophytes stand out asexcellent sources of valuable natural components with diversebiotechnological applications. This study focused on investigating the identityand potential utilizations of a green marine euglenophyte, namely EKoku01,isolated from the brackish Kokuba River in Naha City, Okinawa, Japan.Morphologically, cells of EKoku01 were 12-30m long and 8-10 m wide,displaying dynamic changes during euglenoid motility with a distinctivelystriated pellicular body. Notable features included two uneven flagella, aneyespot, and multiple green discoid chloroplasts. The 18S rDNA molecularphylogeny positioned Ekoku01 within Clade 1 of Eutreptiella, comprisingstrain CCMP389, CCMP-1594, and LIS 2000, which was distantly related toother known marine euglenophytes (i.e., the genus Eutreptia and Clade 2 ofEutreptiella). Based on the morphology and molecular phylogeny, EKoku01was identified as Eutreptiella sp., representing the first recorded species ofthis genus in Okinawa, Japan. EKoku01 showed an optimum growthperformance at 20 oC (max. =0.17 ± 0.037 day-1) and exhibited an unusuallystrong positive phototaxis response towards a light source. Its fatty acid (FAs)profile comprised predominantly saturated FAs (69.8%), polyunsaturated FAs(15.1%, with two omega-3 FAs), monounsaturated FAs (3.6%), and branchedFAs (4.4%), while ~7.1% remaining unidentified. Based on its growthperformance, FAs composition, and light sensitivity, we proposed severalpotential utilizations of this strain (e.g., as a source for nutraceutical,pharmaceutical, food, and aquaculture applications), including the use ofEKoku01 as a model organism for developing an innovative light-driven cellconcentration system based on phototaxis.
Nanochitosan from Manufacture Shells of Mud Crab (Scylla sp.) and Its Application as Acne Patch Luthfiyana, Novi; Ratrinia, Putri Wening; Nusaibah, Nusaibah; Nisa, Mutia Khoirun
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.928

Abstract

Chitosan is a potential raw material for marine cosmetics with antibacterial,anti-inflammatory properties and can stimulate collagen synthesis in theskin. This research aimed to obtain nano-sized chitosan (nanochitosan) frommud crab (Scylla sp.) shells and apply it as a cosmetic acne patch. Theparameters used to characterize nanochitosan are particle size, polydispersityindex, zeta potential and morphology. Meanwhile, the parameters of the Acnepatch produced are characterized by physical appearance and ance bacterialactivity. The results revealed that nanochitosan had an average size of 35.20±1.66 nm, the highest intensity of 11.70±1.25, polydispersity of 0.274±0.10,and zeta potential of 42.9±1.41 mV. The morphology of nanochitosan is in theform of broken rods, not hollow and irregular but uniform. Its elements includecarbon, oxygen, magnesium, aluminum, phosphorus, and calcium. The acnepatches resulting from the addition of 0 mg (K0), 50 mg (K1), 100 mg (K2),and 150 mg (K3) were opaque white, slightly transparent, smooth, elastic,and odorless. The thickness varies, namely, 0.014 ± 0.002 mm (K0), 0.017 ±0.002 mm (K1), 0.022 ± 0.004 mm (K2), and 0.031 ± 0.005 mm (K3). Theweight variations were 0.019 ± 0.001 mg (K0), 0.026 ± 0.005 mg (K1), 0.033± 0.002 mg (K2), and 0.047 ± 0.013 mg (K3). The moisture loss varied,namely, 7.525 ± 0.054% (K0), 3.201 ± 0.487% (K1), 2.741 ± 0.279% (K2), and2.017 ± 0.290% (K3). Acne patches K1, K2, and K3 were proven to be able toinhibit the activity of acne bacteria Propionibacterium acnes (9.5 ± 0.25 mm,10.67 ± 0.76 mm, and 9.17 ± 0.76 mm), Staphylococcus epidermidis (9.33 ±1.15 mm, 13.67 ± 2.02 mm, and 8.67 ± 1.89 mm), and S. aureus (10.33 ±0.25 mm, 11.83 ± 0.76 mm, and 8.66 ± 0.76 mm). This research succeededin obtaining chitosan from crab shells (Scylla sp) in nano size and acnepatches with the addition of nanochitosan effectively inhibited the growth ofacne bacteria.
Characteristics of Trypsin Isolated From Intestines Different of Fish and Correlation Toward Trypsin Activity Nurhayati, Tati; Abdullah, Asadatun; Rahmawati, Septiya; Kurniawan, Riki
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.904

Abstract

Trypsin is a protease that breaks protein peptide bonds. Fish intestines canbe used as an alternative raw material for trypsin. Trypsin enzymes from theintestines of different fish species have different characteristics. This studyaimed to determine the characteristics and stability of trypsin enzyme inNaCl extracted from fish intestines based on differences in fish species. Trypsinactivity was optimal at 50 °C and pH 8, with specific activity values of 0.5993U/mg in rabbitfish, 0.3880 U/mg in sharks, and 0.6964 U/mg in flatfish. Themaximum reaction speed (Vmax) was the highest for trypsin from the intestineof rabbitfish (0.2585 mmol/s), followed by flatfish (0.1042 mmol/s), andshark (0.0599 mmol/s). The lowest Km value was obtained for trypsin fromsharks (0.4084 mM), followed by flatfish (1.0253 mM), and rabbitfish (4.5952mM). Trypsin from the intestines of rabbitfish and flatfish was stable in NaClsolution (concentration 5-30%), as it can maintain a relative activity of morethan 50%. In contrast, trypsin extracted from the intestines of milk fish hada relative activity below 21%. The average molecular weights of the threetrypsin enzymes were 26.8, 27.2; and 21.9 kDa, respectively. Differences inthe type of fish affected trypsin enzyme activity. Flatfish are omnivorous,and rabbitfish, as herbivores, have better enzyme activity values than sharks,as carnivores.

Page 1 of 1 | Total Record : 9

 1 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue Vol 20, No 2 (2025): August 2025 Vol 20, No 1 (2025): May 2025 Vol 19, No 3 (2024): December 2024 Vol 19, No 2 (2024): August 2024 Vol 19, No 1 (2024): May 2024 Vol 18, No 3 (2023): December 2023 Vol 18, No 2 (2023): August 2023 Vol 18, No 1 (2023): May 2023 Vol 17, No 3 (2022): December 2022 Vol 17, No 2 (2022): August 2022 Vol 17, No 1 (2022): May 2022 Vol 16, No 3 (2021): December 2021 Vol 16, No 2 (2021): August 2021 Vol 16, No 1 (2021): May 2021 Vol 15, No 3 (2020): December 2020 Vol 15, No 2 (2020): August 2020 Vol 15, No 1 (2020): May 2020 Vol 14, No 3 (2019): December 2019 Vol 14, No 2 (2019): August 2019 Vol 14, No 1 (2019): May 2019 Vol 13, No 3 (2018): December 2018 Vol 13, No 2 (2018): August 2018 Vol 13, No 1 (2018): May 2018 Vol 12, No 3 (2017): December 2017 Vol 12, No 3 (2017): December 2017 Vol 12, No 2 (2017): August 2017 Vol 12, No 2 (2017): August 2017 Vol 12, No 1 (2017): May 2017 Vol 12, No 1 (2017): May 2017 Vol 11, No 3 (2016): December 2016 Vol 11, No 2 (2016): August 2016 Vol 11, No 2 (2016): August 2016 Vol 11, No 1 (2016): May 2016 Vol 10, No 3 (2015): December 2015 Vol 10, No 2 (2015): August 2015 Vol 10, No 2 (2015): August 2015 Vol 10, No 1 (2015): May 2015 Vol 10, No 1 (2015): May 2015 Vol 9, No 3 (2014): December 2014 Vol 9, No 2 (2014): August 2014 Vol 9, No 1 (2014): May 2014 Vol 9, No 1 (2014): May 2014 Vol 8, No 3 (2013): December 2013 Vol 8, No 2 (2013): August 2013 Vol 8, No 1 (2013): May 2013 Vol 8, No 1 (2013): May 2013 Vol 7, No 3 (2012): December 2012 Vol 7, No 3 (2012): December 2012 Vol 7, No 2 (2012): August 2012 Vol 7, No 1 (2012): May 2012 Vol 6, No 3 (2011): December 2011 Vol 6, No 2 (2011): August 2011 Vol 6, No 1 (2011): May 2011 Vol 6, No 1 (2011): May 2011 Vol 5, No 3 (2010): December 2010 Vol 5, No 2 (2010): August 2010 Vol 5, No 1 (2010): May 2010 Vol 5, No 1 (2010): May 2010 Vol 4, No 3 (2009): December 2009 Vol 4, No 3 (2009): December 2009 Vol 4, No 2 (2009): August 2009 Vol 4, No 2 (2009): August 2009 Vol 4, No 1 (2009): May 2009 Vol 3, No 2 (2008): December 2008 Vol 3, No 1 (2008): June 2008 Vol 2, No 2 (2007): December 2007 Vol 2, No 2 (2007): December 2007 Vol 1, No 1 (2006): December 2006 Article in Press More Issue