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Jurnal Kimia Terapan Indonesia

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 08532788 EISSN : 25277669 DOI : -

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Articles 283 Documents

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KARAKTERISASI BENTONIT PACITAN Buchari Buchari; Muji Harsini
Jurnal Kimia Terapan Indonesia Vol 6, No 1-2 (1996)
Publisher : Research Center for Chemistry - LIPI

Bentonite is high plastivity and colloidal clay which contains smectite(montmorillonite) mineral as major the component. Commercially, there are two kinds of bentonite, which are calcium bentonite and sodium ben-tonite. Results of DTA, XRD and IR analysis on Pacitan bentonite, show that the major component is montmorillonite mixed with a little quartz. Cation exchange capacity (CEC), specific gravity, surface area, pore volume and swelling index of this bentonite are 45,98-62.42 meq/100 gram; 2,14-2,28 g/mL; 641,44-737,29 m /gram; 0,88-1,25 mL/gram and 78,95-163,16 %, respectively. Based on XRD and CEC value Pacitan bentonites are of the calcium bentonite type.

PREPARATION OF IN - HOUSE COCOA FLOUR RMs AND DETERMINATION OF ITS Cd CONTENT BY USING GFAAS Anna E. Persulessy
Jurnal Kimia Terapan Indonesia Vol 11, No 1 (2009)
Publisher : Research Center for Chemistry - LIPI

In-house cocoa flour RMs instead of CRMs was prepared and checked of its homogeneity and stability. Due to CRMs are limited availability in terms of matrices of concern and costly (high production cost) making in-house cocaflour RMs is alternatively used for daily quality control in the laboratory. The analysis method for reliable checking of homogeneity and stability of cadmium content in the in-house cocoaflour RMs was studied. The effects of magnesium nitrate and lanthanum/magnesium nitrate as chemical modifiers in the determination of cadmium in cocoaflour by GFAAS were studied as well. Detection limit of method was estimated to be 0.07 ug L'(calcuiaied as 3Sy;/slope of calibration curve) and calibration curve of working range was linear. The results analysis for candidate of in-house cocoaflour RMs reveals the consistence results and no variability between and within-bottle observedat 95% limit of confidence and stabile during the period of time observed.

UJI POTENSI AKTIVITAS ANTI KANKER EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb.) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) Dede Sukandar; Sandra Hermanto; Emi Lestari
Jurnal Kimia Terapan Indonesia Vol 11, No 1 (2009)
Publisher : Research Center for Chemistry - LIPI

Has been done research to know anticancerpotencial activity from fragrant screw pine leaf extractapplies method Brine Shrimp Lethality Test ( BSLT).Extract is made by the way of macerate to apply threekinds of solvent, that is butanol, ethyl acetate, and etherpetroleum. Toxicity test is done by using prawn larvaArtemia salina Leach which age 48 hours. Toxic effecteach extract is identified with presentase death of prawnlarva applies probit analysis (LC50). Active extract thenis tested its the phytochemistry content and compoundbioa1(fi! suggested applies GC-MS. Result of his (itsshowing ethyl acetate extract to have the character oftoxic ( LC50 : 288,4 ppm). The toxic compounds whichpredietion.implied in ethyl acetate extract is terpenoidsand steroid.Keywords: Fragrant Screw Pine Leaf Extract, BSLT, Artemiasalina LFitokimia, and GC-MS

DOCKING MOLEKUL DENGAN METODA MOLEGRO VIRTUAL DOCKER DARI EKSTRAK AIR Psidium guajava, Linn DAN Citrus sinensis, Peels SEBAGAI INHIBITOR PADA TIROSINASE UNTUK PEMUTIH KULIT Ayik Rosita Puspaningtyas
Jurnal Kimia Terapan Indonesia Vol 15, No 1 (2013)
Publisher : Research Center for Chemistry - LIPI

We have been molecular docking using MolegroVirtual Docker (MVD) on water extract of guava fruit(Psidiumguajava, Linn) and sweet orange (Citrussinensis,Peels) as inhibitor on enzyme tyrosinase withascorbic acid (vitamin C) as positive control to studywhitening agent. Based on the previous studies, the maincontent of the water extract of guava fruit (Psidiumguajava)are 2,6-dihydroxy-3,5-dimethyl-4-0--D-glucopyranosilbenzophenone, 3-hydroxy-2-butanone, and vitamin C, whilethe extract juice of sweet oranges (Citrus sinensis) arelimonene, linalol, and vitamin C. In this study the resultsshowed that the main content of the water extract of Psidiumguajava fruit have better bond as inhibitor on tyrosinase thanCitrus sinensis and vitamin C which can be seen fromMoldock score of Psidiumguajava (-107.806) and Citrussinensis (-76,9593); it meanslower the energyand morestable binding. The IC50 on water extract of guava fruit(psidiumguajava) and sweet orange (Citrus sinensis) were0.26 mM and 31.07 mM, respectively. The hydrogen bonds of2, 6-dihydroxy-3, 5-dimethyl-4-0--D- glucopyranosilbenzophenone with 5amino acid of tyrosinase were Gly 200,Pro 201 , Gly 196, Phe 197, and Asn 205, while limonen,linalool binding 3 amino acids were Gly 200, Phe 197, andAsn 205. Finally, 3D MVD visualization between maincontent of guava and sweet orange water extract can beconcluded that interaction of guava fruit (psidiumguajava)water extract against tyrosinase was more harmonious andstabil than vitamin C and main content of sweet orange(Citrus sinensis) water extract.Keywords: Psidium guajava, Citrus sinensis, tyrosinase, vitamin Cand docking molecule

STUDI TENTANG SPESIES LOGAM Ni DARI BEBERAPA KATALIS PREKURSOR Ni/SiO2 TERKALSINASI DENGAN TPR DAN XRD Achmad Hanafi Setiawan
Jurnal Kimia Terapan Indonesia Vol 5, No 1 (1995)
Publisher : Research Center for Chemistry - LIPI

Three different kinds of Ni-containlng species have been identified in calcined (673°K) Ni/SiO2 catalyst precursors according to their different reducibilities during temperature programmed reduction (TPR). Species A is attributed to an easily reduced species, corresponding to the bulk NiO with little interaction with the silica surface; species B is assigned to a less easily reduced form, due to small NiO particle attached to the silica surface; species C is ascribed to a very difficult to reduce species, reflecting to the strong NiO-silica surface interaction, Nickelhydrosilicate. Species A is obtained by either impregnation and ion exchanged methods, while species B and C are only produced by the ion echange method. The retained Na in the ion exchanged-NaOH catalyst affected a decreasing in the proportion of species A and a growth of the proportion of species C. The maximum value of intensity ratios of "d-spacing-XRD " of d2 and d3 in the reduced precursor Ni/SiO2 indicated the highest of catalyst activity.

PEMBUATAN KERAMIK KORDIERIT DENGAN METODE SOL-GEL DAN KARAKTERISASI-NYA Silvester Tursiloadi
Jurnal Kimia Terapan Indonesia Vol 10, No 1-2 (2000)
Publisher : Research Center for Chemistry - LIPI

Sol-gel method and melt-quenching as a reference methodwere studied for preparing a-cordierite phase. The solution ofcordierite precursor was prepared from a mixture of Si(O-Et),Al(O-Bu'), and Mg (O-EI)2 in 2-methoxyelhanol. The alkoxidesolution thus obtained was hydrolyzed with NH4OH-water. Thegel obtained was aged 80'C. After aging for more than one day,the products were dried at 120'C for 24 hours in dry oven. Allthe gel powders obtained were heat-treated up to 1050°C. Theirphase transformation was studied by using a DTA and X-raydiffraction. It was found that different crystallization behaviorswere shown by the Al/Si ratio in gel powders. As the amount of.41 atoms decreased the exothermic peak of DTA due tocrystallization shifted to lower temperature and the initialtram/ormation of a-cordierite phase front u-cordierite took placeby heat-treating at 900'C for 1 hour. However, the completetram/ormation of single a-cordierite phase took place at highertemperature than Al-rich samples. Although the calcination wasdone at 1050·C for 1 hr, the phase of u-cor dierite was stillobserved. Transformation of u-cordierite to a-cordierite phasefor Al-rich samples showed al a higher temperature comparedfor Al-poor samples, although the complete transformation tookplace at a relatively low temperature, compared with solid statereaction.The increase in Al concentration will increase the density ofsamples. The density of all samples were increased up to thecalcination temperature of 950·C, and the highest density wasobtained at 1050·C. The sample which was prepared by 'meltquenching'method, showed that the density of the sample was almostthe same although the calcination temperature was increased up tomore than 900'C. After calcination at 1050'C, the Al-rich samplehas a density of around 3,32 g/cm3 (93% of density standardcordierite).

PREPARASI PEREAKSI KIT IMMUNORADIOMETRlCASSAY FREE PROSTATE SPECIFIC ANTIGEN UNTUK DETEKSI KANKER PROSTAT Puji Widayati; Gina Mondrida; Sri Setiyowati; Agus Ariyanto; V. Yulianti Susilo; Wening Lestari
Jurnal Kimia Terapan Indonesia Vol 15, No 2 (2013)
Publisher : Research Center for Chemistry - LIPI

Prostate Specific Antigen (PSA) is a glycoprotein with a molecular weight of approximately 34,000 daltons serine protease secreted exclusively by prostatic epithelial cells that lining acini and prostate gland. Increased of PSA levels can be caused by prostate cancer or benign prostate enlargement (benign prostatic hyperplasia, BPH). PSA in the blood was found in the free condition (free PSA) and most of the bound protein (complexed-PSA, c-PSA). Measuring levels of PSA was found in the blood can be done by several methods such as by immunoradiometricassay (IRMA) methods or ELISA methods. IRMA method is one of immunoassay techniques using radionuclides ,/' 125 oJ I as a tracer, so the sample in small 13 quantity can be detected The purpose of this study was obtained PSA reagent kit that includes 1251labeled PSA as a tracer, PSA coated tube and PSA standard that requirements of the kit, then it can be optimized assay design, that eventually PSA reagent kit can be used for early detection of prostate cancer. It has been done labeling of Mab PSA using 125 1with reaction time was 90 seconds, amount of PSA MAb was 75 ugram and the activity of Na_ 125I was 1000 flCi. Preaparation of PSA coated tube using 0.05 M Na2C03 solution, at pH: 9.6 with volume was 250 ml., standard PSA with 0.025 Mphosphate buffer at pH 7.4 containing 5% BSA and 0.1% NaN3, and resulting at 1,25% and 14,12% respectively of NSB and BIT that requirement of the kit.Keywords: Prostate cancer, PSA, IRMA,NSB, Maximum Binding

PENGARUH CARA PENAMBAHAN ENZIM GLUKOAMILASE DAN ION LOGAM ALKALI DAN ALKALI TANAH PADA PROSES SAKARIFIKASI PATI SAGU A. T. Karossi; Agus Muchliawan; Linar Z.Udin; A. Sidik
Jurnal Kimia Terapan Indonesia Vol 5, No 1 (1995)
Publisher : Research Center for Chemistry - LIPI

Production of glucose syrup enzymatically employs both alpha-amylase and glucoamylase. Alpha amylase acts during liquifaction while glucoamylase in the saccharification process. In the present study the glucoamylase was obtained from Rhyzopus oryzae fermentation for five days using 4 liter scale LKB fermentor. The influence of single stage and multiple stage additions of glucoamylase on alpha amylase liquified sago starch indicated no significant difference on the saccharification. The presence of 0.2 - 0.8 mM Li+, Na+, K+, Mg++, Ca++ and-Bar+ salts enchanced the glucoamylase activity whereas at the level of 1 mM they acted as inhibitors. The results of HPLC analysis of the saccharification product showed that the glucoamylase hydrolysed 83.3% of the sago starch yielding free glucose.

MELACAK PELAKU TERORISME MELALUI PENENTUAN KANDUNGAN KATION DAN ANION DALAM SAMPEL HASIL PENCUCIAN TELAPAK TANGAN PELAKU DENGAN TEKNIK KROMATOGRAFI ION Muhammad Amin
Jurnal Kimia Terapan Indonesia Vol 16, No 1 (2014)
Publisher : Research Center for Chemistry - LIPI

Telah dilakukan analisis kandungan kation (Na+, NH4+, K+, Mg2+ dan Ca2+) dan anion (NO2âˆ’,Clâˆ’, ClO3âˆ’, NO3âˆ’, and SO42âˆ’)menggunakan instrumen kromatografi ion terhadap 80 sampel yang berasal dari hasil pencucian telapak tangan penjual petasan/kembang api dan hasil pencucian telapak tangan orang yang diketahui tidak memegang sampel petasan/kembang api sebelumnya. Petasan, kembang api dan korek api adalah 3 jenis bahan kimia yang berpotensi digunakan sebagai bahan peledak. sejumlah5 mM asam tartrate digunakan sebagai eluen untuk pemisahan kelima jenis kation menggunakan kolom penukar-ion kation dan 1 mM asam trimellitik digunakan sebagai eluen untuk pemisahan kelima jenis anion menggunakan kolom penukar-ion anion. Hasil analisis menunjukkan bahwa konsentrasi beberapa kation (Na+, NH4+ dan K+) pada sampel hasil pencucian telapak tangan penjual petasan/kembang api meningkat 2â€“3x lipat dari sampel hasil pencucian telapak tangan yang tidak memegang sampel. Ion K+ adalah kation penyusun utama dalam ketiga sampel. Ion NO3âˆ’ adalah anion penyusun utama untuk sampel petasan dan kembang api, dan ion ClO3âˆ’ adalah anion penyusun utama korek api. Terlihat bahwa instrumen analisis kromatografi ion dapat menjadi alternatif utama dalam membantu dan menyiapkan data analisis kandungan kation dan anion dalam 3 jenis sampel di atas untuk maksud melacak pelaku terorisme.Kata kunci : Kromatografi ion, Pelaku teroris, Telapak tangan, Kation, AnionÂ The analysis of cations (Na+, NH4+, K+, Mg2+ and Ca2+) and anion (NO2â€“, Clâ€“, ClO3â€“, NO3â€“ and SO42â€“) have been done using ion chromatography method for 80 samples from palm washing of the seller of firecrackers/fireworks, the palm washing known that the person did not touch/hold the sample previously and potentially explosive simulation sample. Firecracker, fireworks, and matches are 3 types of chemicals used as simulation that could potentially be used as explosives. When 5 mM tartaric acid and 1 mM trimellitic acid were used as the eluents, 5 cations and 5 anions could be separated on the cation-exchange column and anion-exchange column, respectively. The analysis results showed that the concentrations of Na+, NH4+, and K+ ions in samples of hands washing from the sellers increased 2-3x comparedto those did not hold the samples previously. K+ ion is the main cation contained in the three types of samples. NO3â€“ion is the main anion contained in firecracker and fireworks while ClO3âˆ’ ion is the main anion contained in matches sample. It was concluded that ion chromatography method as an analytical instrument may be a good alternative to prepare the initial data and content analysis of cations and anions in the above three types of samples for counterterrorism purposes. Keywords: Ion chromatography, Terrorist, Palm washing, Cations, Anions

PENGARUH BERAT MOLEKUL ADITIF PADA PENAMPANG MELINTANG DAN KARAKTERISTIK MEMBRAN SERAT BERONGGA POLISULFON, YANG DIAMATI DENGAN ALAT SCANNING ELECTRON MICROSCOPY Syahril Ahmad
Jurnal Kimia Terapan Indonesia Vol 10, No 1-2 (2000)
Publisher : Research Center for Chemistry - LIPI

Cross section of polysulfone membranes hollow fiber spun bydry - wet process have been studied. Composition of membraneobserved composed of 16% Polysulfone (PS) , 64% dimethylAcetamide (DMAc) as solvent and 18% Polyetylene glycol (PEG)as additive. Molecular weight of polyetylene glycols used were 200,300 and 400 Daltons. Fiber of membranes were charaterized bydextrane solution to find rejection coefficient and molecular weightcut - off of the membrane. Cross sections of hollow fiber membraneswere observed by Scanning Electron Microscopy (SEM).Results of the experiment show that, molecular weight ofpolyethylene Glycol additif used in spinning of hollow fibermembrane affects form of cross section of substructure andmolecular weight cut - off the membrane obtained. This researchwas aimed to predict the pore size of membrane made based onmolecular weight of additive used.

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