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Jurnal Kimia Terapan Indonesia

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 08532788 EISSN : 25277669 DOI : -

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Articles 283 Documents

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STUDI PEMISAHAN Co(II), Cu(II), Fe(III) DAN Ni(II) SECARA EKSTRAKSI PELARUT DENGAN ZAT PENGKOMPLEKS NATRIUM DIETILDITIOKARBAMAT Soefjan Tsauri; K. Anom W; Buchari Buchari
Jurnal Kimia Terapan Indonesia Vol 3, No 1 (1993)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v3i1.278

Sodium diethyldithiocarbamate (NaDDC) as a chelating agent reacts unselectively with cobalt(II), copper(II), iron(III) and nickel(II). Their maximum wavelengths are very similar in carbon tetrachloride solution and hence these complexes could not be determined by UV/VIS spectrophotometer without prior separation. A scheme of separation has been found for qualitative analysis of cobalt(II), copper(II), iron(III) and nickel(II) at a certain pH using a specific masking agent. It was found that pH 9 turned out to be the best condition for iron(III) masking but neither for cobalt(II), nor for copperill] and nickel(II). The addition of 10 mL HCI 12 N to the complex solutions of Co(II), Cu(II) and Ni(II) would remove Cu(II) and Ni(II) complexes from CC14 to water phase whereas Co(II) complex was still in the CCl4 phase. Cu(II) and Ni(II) ions in the water phase were then treated with 10 mL Titriplex 8,66 x 10-5 mole at pH 9 in which Ni(II) was masked but not Cu(II).

STANDARDISASI EKSTRAK PEGAGAN, CENTELLA ASIATICA SEBAGAI OBAT HERBAL TERSTANDAR HEPATOPROTEKTOR Puspa Dewi N Lotulung; Sri Handayani; Teni Ernawati; Tri Yuliani; Nina Artanti; Tjandrawati Mozef
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v17i2.34

Herbal medicinal products would be affected by the quality of raw materials. In turn, the quality of raw material will also be influenced by various factors such as soil conditions, cultivation, post-harvest processing, and the processing of raw materials into crude drug or extract. Therefore, in order to make good herbal medicines, it is necessary to make standardization of herbal extracts that produced herbal medicines that have the same quality and functions of effectiveness in each process. From preliminary studies that have been done, Centella asiatica is one of the potential plants as a source of hepatoprotective compounds. Test in vivo and in vitro against Centella asiatica extracts have shown very good results. Ethyl acetate extract with 17.5 mg/kg of doses body weight and butanol 228.8 mg/kgof doses body weight has been applied for in vivo test using mice induced by CCl4; theydemonstrated hepatoprotective effects. Ethyl acetate extracts were able to reduce levels of the enzyme alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by 56 % and 44 % respectively while butanol extract can reduce the enzymes AST levels by 3%. Standardizationof Centella asiatica extract performed in this study was the characterization of the extract in the form of non-specific and specific parameters corresponding to the reference of PPOMN (Ministry of health Republic of Indonesia, 2000) such as levels of drying shrinkage, ash content, total plate count microbial contamination, levels of water-soluble compounds, levels of compounds that are soluble in ethanol, phytochemical test, total phenolic content, total flavonoid content and the determination of Pb and Cd weight.The results showed that non-specific parameters for the ethanol extract of Centella asiatica were requirements based on Herbal Pharmacopoeia in 2008 which includes parameters such as determination of shrinkage on drying â‰¤ 10%, ash content â‰¤ 16.6% and negative microbial contamination. Specific parameters for the ethanol extract of Centella asiatica have met the requirements of Herbal pharmacopeia in 2008.Keywords: Centellaasiatica, hepatoprotective, standardized herbal medicine, specificparameters, and non-specific parameters

PENERAPAN MEMBRAN MIKROFILTRASI PADA PEMURNIAN EKSTRAK KALDU KACANG HIJAU (Phaseolus radiatus L.) SEBAGAI FORTIFIKAN PRODUK MAKANAN Aspiyanto Aspiyanto; Agustine Susilowati
Jurnal Kimia Terapan Indonesia Vol 12, No 2 (2010)
Publisher : Research Center for Chemistry - LIPI

In preparation of mung bean (Phaseolus radiatus L.) broth extract asfortificant agent offood products via purification by means of microfiltration (MF) membrane, operation conditions have important role. Application of MF membrane modul is afurther process of purification from small scale (Stirred MF cell, 30.175 cm2 of membrane area) to bench-scale (Cross-flow MF modul, 360 cm2 of membrane area). The main goal of this work was to find out effect of flow rate of material and operation pressure on compositions of concentrate/retentate and permeate from mung bean broth extract as savoury (umami)-based fortificant agent offood products. Preparation of mung bean broth extract was conducted by introducing broth extract of crude mung bean to Cross-flow MF modul at pump motor frequency of l0 and 20 Hz, room temperature and operation pressure of 2, 4 and 6 bar, respectively. Investigation was performed on permeate flux value, and analysis was conducted on concentrate/retentate and permeate, covering on total solids, dissolved solid, total protein, dissolved protein, N-amino and salt. The result of experiment showed that pump motor frequency and operation pressure affect on concentrate/retentate and permeate compositions. Increase of pump motor frequency and operation pressure increase permeate composition, but they tend to be almost similar on concentrate/retentate of mung bean broth extract. Treatment combination at pump motor frequency of 20 Hz and operation pressure of 2 bar generated the best composition of concentrate/retentate as hydrolyzed vegetable protein (HVP) with concentrations of total solids of 3.98 %, soluble solid of 6.05 %, total protein of 5.65 %, dissolved protein of 5.3 mg/mL, N-amino of 0.95 mg/mL and salt of 2.04 %, while permeate gave flux value of 32.19 L/m2.hour with concentrations of total solids of 5.74 %, soluble solid of 5.04 %, total protein of 4.51 %, dissolved protein of 4.05 mg/mL, N-amino ofl.08 mg/mL and salt of 2.2 %, and had useful potency asfood savoury flavor.Keyword : Mung bean (Phaseolus radiatus L.) broth, microfiltration (MF), concenirate/reieniaie, permeate, hydrolysate.

ISOLASI ENZIM HORSERADISH PEROKSIDASE (HRP) DARI KULTUR SEL DAUN ARMORACIA LAPATIFOLIA DENGAN CARA FRAKSINASI MENGGUNAKAN AMONIUM SULFAT A. T. Karossi; S Pudjiraharti
Jurnal Kimia Terapan Indonesia Vol 12, No 1 (2010)
Publisher : Research Center for Chemistry - LIPI

Isolation of HRP ( Horseradish Peroksidase ) from Armoracia lapatifolia plant cell culture was conducted by fractionation using ammonium sulfate. Optimation of thefractionation process was carried out by concentrating the crude enzyme, investigating the fractionation stages and precipitation with ammonium sulfate. Two-stage fractionation namely with 0 - 40% and 40 - 85 % saturation of ammonium sulfate gave better results in terms of enzyme activity. In 0 - 40% saturation, the purity was 23fold with 11.4 %yield. The 20 highest yield, 60% and purify of 43 folds were showed by fraction which were obtained at fractionation 40 - 85 % saturation.Keyword: Horseradish Peroksidase, Annoracia Lapatifolia

PEMURNIAN DAN KARAKTERISTIK DEXTRANASE BACTEROIDES RUMINICOLA SUBSP BREVIS Tami ldiyanti
Jurnal Kimia Terapan Indonesia Vol 4, No 2 (1994)
Publisher : Research Center for Chemistry - LIPI

The dextranase of B.ruminicola subsp. brevis from bovine rumen has high ability to degrade the D - (1,6) a-glucosidic linkages of dextran substrate. Purification of crude dextranase was done by using ion-exchange chromatography and electrophoresis. It was observed that the purification until ion exchange step by microcrystal cellulose column increased theactivity of dextranase by 400 fold. The purified dextranase was most active at pH 5.5 and 40°C-45°C. The enzyme was strongly inhibited by metal ions such as Cu++,Fe++ and Hg++. The major product in early stage of dextran hydrolysis which were analyzed by paper chromatography showed dextranase of B.ruminicola to be an endotype enzyme.

Sintesis dan Uji Mekanik Epoksi Termodifikasi Poliuretan Berbasis Ester Gliserol Monooleat M Ghozali; L R Fauzi; E Triwulandari
Jurnal Kimia Terapan Indonesia Vol 18, No 01 (2016)
Publisher : Research Center for Chemistry - LIPI

Modifikasi epoksi telah dilakukan untuk mengatasi kelemahan epoksiterutama dalam hal kekuatan tarik. Epoksi termodifikasi poliuretan (ETP)merupakan salah satu cara yang telah dilakukan. Pada penelitian inimodifikasi epoksi menggunakan poliuretan dilakukan dengan caramenambahkan senyawa ester gliserol monooleat sebagai poliol. Sintesis estergliserol monooleat (GMO) dilakukan melalui reaksi esterifikasi gliseroldengan asam oleat dengan katalis asam sulfat dengan rendemen 80%.Sintesis Epoksi Termodifikasi Poliuretan Berbasis Ester Gliserol Monooleat(ETPGMO) dilakukan dengan cara mereaksikan, epoksi, tolonat dan GMOpada suhu 50Â°C selama 30 menit, menghasilkan rendemen 80%.Karakterisasi gugus fungsi dan struktur GMO dan ETPGMO dilakukandengan FTIR dan NMR. Pembuatan film ETPGMO dilakukan denganmenambahkan hardener. Hasil analisa sifat mekanik menunjukkan bahwakekuatan tarik ETPGMO lebih tinggi dua kali lipat dibandingkan epoksi yangdisebabkan hadirnya poliuretan.Â

PRODUKSI ALFA-AMILASE OLEH ASPERGILLUS ORYZAE DALAM MEDIA PATI SAGU (Metroxylon sp.) S. Pudjiraharti; L. Z. Udin; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 7, No 1-2 (1997)
Publisher : Research Center for Chemistry - LIPI

The production 0f alpha-amylase in sago starch media by A. oryzae have-been performed in Biostat-B stirred tank fermentor with working volume of 2 L. The condition was adapted from the fermentation using Biotech fermentor: working volume 4 Liters, temperature 27°e, aeration 0,75 wm and agitation of 300 rpm. The concentrations of inoculum added into the medium were 2,5 - 3% v|v. The maximum enzyme speslfic activites around 300-460 U|g protein was obtained at fermentation using inoculum concentration of 2,5%, while the maximum enzyme specific activity of 850 U|g protein was also obtained at fermentation using inoculum concentration of 3%. The maximum enzyme specific activity was achieved at day 5 or 6 of fermentation.Fermentation using various concentrations of inoculum in erlenmeyer flask scale was carried out to investigate the inoculum concentration which resulting maximal enzyme activity. The concentrations used were 5.0%; 7.5%; 10%; and 12.5% v|v. Fermentation was done at 30°C and agitation of 120 rpm. The highest enzyme activity of 12,640 Vlg protein was resulted at fermentation with inoculum concentration of 12.5% v|v at day-5. Application into fermentator two liters at temperature 30°C, aeration 1.5 vvm and agitation of 500 rpm showed enzyme production in earlier time (one day fermentation) to achievedenzyme activity of around 1000-1300 U|g protein.

PENGARUH KONSENTRASI PENGINDUKSI METANOL SERTA"SUMBER KARBON SORBITOL DAN MONITOL TERHADAP PRODUKSI a-AMILASE Saccharomycopsis fibuligera R64 DALAM Pichia pastoris Shabarni Gaffar; Dani Permana; Diana P Rahmawati; Triana N Meirina; Abu Bakar M.I. Syihab; Safrl Ismayana; Toto Subroto; O Suprijana
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Pichia pastoris has been widely used as host for heterologous protein expression for commercial purposes. The advantages of P. pastoris as protein expression host is its ability to grow with high cell density and the presence of a gene promoter that canbe induced tightly, named AOXI encoding alcohol oxidase. Improvement of recombinant protein production under control of AOXI promoter in P. pastoris expression system is still a major concern. Optimization of methanol concentration as inducerand addition of carbon sources, is one of the strategies to improve the expression level. This research aims to study the effect of methanol as inducer as well as sorbitol and mannitol as additional carbon source to the expression level of recombinantSaccharomycopsis fibuligera a-amylase (Sfamy) by P. pastoris (Mut). Sorbitol and manuot known as non-repressive carbon source, to increase the growth ofP. past oris, but not inhibit the AOX1 promoter and foreign proteins expression. Sfamy was expressed in P. pastoris GS115 (His-, Mut) with addition ofcarbon source, sorbitol and mannitol saparately to the expression medium. The result showed that, the optimum concentration" of methanol inducer for Sfamy production is 0.75%. The addition of sorbitol or mannitol increased Sfamy production. Concentrations of sorbitol and mannitol 2% with0.75% methanol inducer increase the secretion level of recombinant Sfamy 2.13 times and 1.94 times respectively, compared with no additional carbon source. This result indicated that addition of carbon source can improved recombinant protein production by P. pastoris, and the used of sorbitol as additionalcarbon source is more effective compared to mannitol.Key words: a-amylase. Pichid pastoris, methanol, sorbitol,mannitol.

PERBANDINGAN Cr-51 (III) DAN Cr-S1 (VI) ANORGANIK PADA HASIL IRADIASI Cr(CO)s DAN Cr(C5H7O2)3 DENGAN NEUTRON TERMAL Sunarhadijoso Soenarjo; Said Adam; Iswandi Idris
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

Thermal neutron irradiation on Cr(CO)6 and Cr(C5H7O2)3 target compounds had been conducted with an average neutron flux of about 2.8 x 10(12) n.cm(-2).der(1). Both post-irradiated target compounds gave inorganic radiochromium in oxidation states of +3 and +6 which were separated by solvent extraction method: For radioactivity measurement, the trivalent species was separased from the hexavalent by hydroxide precipitation using K2CrO4 and Cr(NO)3. 9H2O carriers. The inorganic chromium content was chemically determined by spectrophotometric method without adding arry carriers. The activity of the trivalent inorganic chromium produced from Cr(CO)6 irradiation was higher than that of the hexavalent ones, but in the case of Cr(C5H7O2)3 irradiation; the activity of the hexavalent species was higher. In both cases, the specific activity of the trivalent species was higher than that of the hexavalent species. The specific activity of total inorganic chromium obtained from the irradiation of Cr(CO)6 was higher than that of Cr(C5H7O2)3.

EVALUASI NILAI GIZI SECARA BIOLOGI DENGAN TETRAHYMENA PYRIFORMIS GL, UNTUK BEBERAPA INTISARI JENIS KACANG·KACANGAN T Lindajati; Yetty Mulyati I; R. H Hari
Jurnal Kimia Terapan Indonesia Vol 1, No 2 (1991)
Publisher : Research Center for Chemistry - LIPI

Bioassay using Tetrahymena pyriformis GL was applied to evaluate the nutritive value of soybean (Glycine max), lablab (Lablab purpureus), lamtoro sabrang (Acacia spy, orok-orok (Crotalaria sp) and turi (Sesbania grandiflora). The beans were tested either before or after being fermented with Rhizopus oligosporus Ub. Pepsin was used ill predigestion of the samples for the assay. It was found that cooked and fermented soybean, lablab and orok- orok had the relative nutritive value (RNV) approximating that of casein standard which had been set at a value of 100. Cooked lamtoro sabrang and turl had the RNV lower than that of the casein standard, i.e. 67 % and 22 % respectively before fermentation with Rhizopus oligosporus Ub. However, despite the fact that no change was found ill the RNV of lamtoro sabrang there was an improvement in the RNV of turi after fermentation, i.e from 22% t044 %.

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