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Biochar Sebagai Penyimpan Karbon, Perbaikan Sifat Tanah, dan Mencegah Pemanasan Global : Tinjauan
Rakhman Sarwono
Jurnal Kimia Terapan Indonesia Vol 18, No 01 (2016)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v18i01.44
Terjadinya pemanasan global disebabkan karena makin tingginya konsentrasigas rumah kaca (GRK) di atmosfir. Pemanasan global akan memberidampak yang buruk terhadap kehidupan mahluk hidup di bumi ini. GRKyang sangat berpengaruh terhadap naiknya temperatur atmosfir bumi adalahgas CO2 [karbon dioksida], NOx, metan dan freon. Salah satu GRK yangmenjadi perhatian adalah gas CO2, karena gas CO2 kebanyakan dari kegiatanmanusia seperti pembakaran bahan bakar fossil yang jumlahnya selalumeningkat setiap tahunnya. Sejak revolusi industri sampai sekarang terjadiakumulasi gas CO2 di atmosfir yang semakin besar, dengan konsentrasi CO2sebesar 390 ppm. Hal ini ditengarai sebagai penyebab terjadinya pemanasanglobal dan perubahan iklim. Untuk menahan laju penambahan konsentrasigas CO2 di atmosfir perlu adanya cara untuk mengurangi karbon di atmosfir.Salah satu cara untuk mengurangi karbon dioksida CO2 di atmosfir adalahdengan mengkonversi biomasa menjadi biochar dan selanjutnya biochardimasukkan kedalam tanah sebagai pemuliaan tanah agar kelestarianpertanian bisa berlanjut. Bila biochar bisa diproduksi dalam jumlah yangbesar yang setara dengan jumlah karbon yang masuk ke atmosfir, maka akanterjadi keseimbangan antara gas CO2 yang masuk dan keluar atmosfir.Penyimpanan karbon sebesar 1 gigaton pertahun selama 40 tahun akanmenghambat penambahan konsentrasi gas CO2 di atmosfir. Sehingga lajupenambahan gas rumah kaca bisa dicegah.
UJI BANDING METODE ANALISIS KONVENSIONAL DAN INSTRUMENTAL UNTUK PENENTUAN KALSIUM DAN FOSFOR
Hendro Santoso M
Jurnal Kimia Terapan Indonesia Vol 7, No 1-2 (1997)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v7i1-2.226
Analysis of calcium and phosphor has been done by using conventional and instrumental methods. Lorenz gravimetric method was employed to determine phosphor, whereas permanganometric methods used to determine calcium. Spectronic molibdovanadophosphate and atomic absorption spectrophotometric methods were used as control for phosphor and calcium respectively. Samples analysed in this study were molasses and driedfaeces.Results show that there is no significant effect on analysis phosphor by using both Lorenz gravimetric method and its control. Similarly, permanganometrlc methods result in the same value with its control.
BIOSENSOR UNTUK ANALISIS UREA BERDASARKAN PADA APLIKASI ENZIM UREASE DAN ELEKTRODA TUNGSTEN
Bambang Widihastono
Jurnal Kimia Terapan Indonesia Vol 3, No 1 (1993)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v3i1.298
This study describes the development of a potentiometric biosensor for the determination of urea. The sensor was constructed by combining a tungsten wire with an enzyme, urease, physically immobilized in polyvinylchloride (PVC). The fabrication of the sensor was carried out by coating a tungsten wire with the immobilized urease. The changes in pH resulted from the enzyme-catalyzed hydrolysis of urea is measured with the tungsten electrode and shown to be proportional to the log of concentration of urea in sample solutions over a limited concentration range. Urea Sensor prepared in this study gives an almost linear response with the log of concentration in the range of 0.1 - 10.0 mM, and the calibration slope wasfound to be 52 mV Vper decade change in urea concentration.
Simple Purification of Vetiver Oil by Multiglass Plate System for Quality Improvement
Evi Yanto;
Egi Agustian;
Anny Sulaswatty
Jurnal Kimia Terapan Indonesia Vol 18, No 02 (2016)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v18i02.86
Vetiver oil is one of essential oil from Indonesia which no synthetic substitute is available. The drawback of vetiver oil from Indonesia is smoky odor and dark appearance. To increase added value of Indonesian vetiver oil, therefore it is need method with cheap production, simple and easy to operate. Multiglass plate system (MGS) is one method to increase added value of vetiver oil. The aim of this research is to improve the vetiver oil quality in terms of appearance, odor and the physicochemical properties by MGS method and also optimize the operation condition on the vetiver oil yield and total vetiverol contents. The process parameters used on this research are to optimize the flow rate, angular and amount of glass plate. As a result, was obtained the optimum condition in 0.38 ml/sec of flow rate, 3o of glass plate angular and 5 of glass plate to increase 15% of total vetiverol contents, while the yields decreased until 5.6%. Refined vetiver oil by MGS are almost meet fulfill with Indonesia National Standard number 06-2386-2006 except total vetiverol contents and odor. Even though, the total vetiverol content increased and the odor became slightly smoky compare to raw material. Based on the results, MGS method can give higher purification of vetiverol of increase vetiver oil quality with easy to handle, low cost operation and also can moved to anywhere due to portable unit and mobile system.
SOL-GEL PROCESS FOR THE MANUFACTURING OF TRANSLUCENT LEAD ZIRCONATE TITANATE GEL-MONOLITH
Silvester Tursiloadi;
Hiroaki Imai;
Hiroshi Hirashima
Jurnal Kimia Terapan Indonesia Vol 6, No 1-2 (1996)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v6i1-2.231
Translucent Lead Zirconate Titanate (PZT) gel-monolith was prepared by partially hydrolyzing metal alkoxides solution which modified with acetylacetone(acacH). Metal alkoxides, lead di-i-propoxide, zirconium tetra-nbutoxide and titanium tetra-i-propoxide were used as starting materials. In Infrared spectra for the translucent monolithic gel after aging at room temperature for several days or drying at 90°C for 18h, the most significant feature is the presence of band at around 1554 cm-1 which can be assigned to the v (C-O) and v (C-C) vibrati-ons of acetylacetanate group coordinated to the metal cations Ti and Zr. The diffraction peaks of PbO were found after heating at 300°C for 2h. After heating at 450 °C for 2 h, diffraction peaks of pyrochlore Pb2 Ti206 and perovskite PZT phase were observed. The diffraction peaks of PbO and pyrochlore phase disappeared after heating at 600°C, and tetragonal perovskite phase was stable up to 1000 °C. The diffraction peaks of perovskite phase were also found after heating at 430 ° for 24 h. The density of the compacted pulverizedgel after heating at 1000°C for 30 min. was 6.9 g/cm3 , about 86% of the theoretical value.
ANTI BREAST CANCER ACTIVITY OF ETHYL ACETATE EXTRACT OF FERMENTATION BROTH EMPLOYING ENDOPHYTIC FUNGI TAXUS SUMATRANA ISOLATES
A.T. Karossi;
A Poniah;
L.Z. Udin;
S Rieny
Jurnal Kimia Terapan Indonesia Vol 11, No 1 (2009)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v11i1.172
Fermentation broths of TSC 18 and TSC 22 fungi isolates obtained from Taxus sumatrana plant were investigated for their in vitro anti breast cancer activity against T47D cell line. The isolates were further identified by PCR and sequencing methods. In the fermentation experiments, the growth curves were constructed by plate count method and the pH change were recorded, while glucose and protein dynamics of the broth were determined by Nelson-Somogy and Lowry methods respectively. Anti-cancer activity was assessed by SRB (Sulforhodamine B)method. The investigation indicates the fermentation broth both isolates possessed the anticancer activity with lese of 42.6 ppm for TSC 18 and 55.7 ppm for TSC 22. Cisplatin which is used as a reference compound gave ICso of 15.9 ppm . The PCR and tree view program proved that TSC 18 is Phomopsis sp. strain MAFF-238472 and TSC 22 is Coprinopsis cinerrea strain NBRC30628.Keyword: Anticance, Isolates TSC 18 and TSC 22 Taxus Sumatrana.
PENGARUH PENGGUNAAN GARAM NATRIUM DAN KALIUM METABISULFIT PADA MUTU SALE PISANG AMBON
ANNY SULASWATTY;
ROESTAMSJAH ROESTAMSJAH
Jurnal Kimia Terapan Indonesia Vol 1, No 2 (1991)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v1i2.303
Techniques of improving the colour, aroma, texture and storage life of pisang sale or dried banana (Musa paradisiacal was conducted through sulphiting and drying. In this study, the sulphiting of ball all a fruit was done by soaking the fruit in O.S% sodium meta bisulfite solution (NazSzOs) or O.S% potasium metabisulfite solution (K2S2O5) for five minutes. Dehydration was conducted at 6O°C for 44 hours in a tray dryer. The dried banana was put in plastic bag of O.5 mm film thickness and stored in a rack covered with transparent plastic film at ambient temperature (25° ± 2°C) and relative humidity of ±70%. The experiment was done using random block design. Analysis of the dried banana products included chemical, microbiological an d organoleptic methods after storage for one day, three weeks, six weeks and 12 weeks. The results indicated that the artificial drying coupled with the metablsulphite treatment could improve the quality and shelf life of the dried banan a produced. The product quality characteristics include: water content (23-27%), total reducing sugar (41-49%), SO2 (3-S fpm), color/optical density (0,3-0,7); and total colony count (7-7.5 x 10(3)) per gram sample for the products stored up to 12 weeks.
PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI LAPIS TIPIS DENSITOMETRI UNTUK PENETAPAN KADAR TEOFILIN DAN EFEDRIN HIDROKLORIDA SECARA SIMULTAN PADA SEDIAAN TABLET
Lestyo Wulandari;
Yuni Retnaningtyas;
Diyanul Mustafidah
Jurnal Kimia Terapan Indonesia Vol 15, No 1 (2013)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v15i1.100
The combination of Theophylline and Ephedrine isoften used to induce an additive effect in alleviating thesymptoms of respiratory disorders. This research is aimed todevelop a Thin Layer Chromatography (TLC) densitometrymethodfor the simultaneous determination of Theophyllineand Ephedrine hydrochloride in tablet dosage form. Themethod employed TLC aluminium plates precoated withsilica gel 60F254 as the stationary phase and the mixture ofethyl acetate: glacial acetic acid: aquabidest (11: 5 : 1 v/v)asthe eluen system. For detection of Ephedrinehydrochloride, the TLC plate must be re-eluted with 0,5%ninhydrin solution in aquabidest, and then heated at 105°Cfor 15 min. Quantitative evaluation was performed bymeasuring the absorbance-reflectance of the analyte spot at279 nm and 505 nm for Theophylline and Ephedrinehydrochloride, respectively. validation of the method showthat TLC Densitometryis selective and specific,showed agood linearity, precise, and accurate and can be used forroutine analysis of tablet in industrial quality controllaboratories.Keywords:TLCDensitometryV, alidationT, heophyllineE, phedrinehydrochloride
KLON GEN PENISILIN ASILASE PADA COSMID pHC79
Linar Z. Udin;
Hadi Sutedjo
Jurnal Kimia Terapan Indonesia Vol 6, No 1-2 (1996)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v6i1-2.236
Penicillin acylase plays an important role in the catalysis of benzylpenicillin hydrolytic reaction, prodeucing 6-aminopenicilanic acid (6- APA), a precursorfor the formation of penicillinderivatives. Cloning of the penicillin acylase gene of Escherichia coli B130 chromosomal DNA on pHC79 cosmid vector to increase the enzyme activity has been investigated. The cloning was cooducted with several steps, including isolation of the chromosomal DNA. digestion by restriction enzyme, ligation by T4-DNA ligase, transformation of the recombinant DNA, and selection of the transformans. Microbial assay utilizing Serratia marcescens was carried out for screening the penicillin acylase colony, whereas the determination of the enzyme activity was examined based on Kornfeld method. From 2070 colonies screened by S. marcescens, only 4 positive colonies were obtained. The enzyme activity of these colonies was 4-6 fold higher than the penicillin acylase activity from E. coli B 130.
ISOLATION AND IDENTIFICATION OF SIMIARENOL FROM THE LEAVES OF FICUS AURANTIACEA GRIFF (MORACEAE)
L. B.S. Kardono;
Lenny Sutedja;
H. H.S. Fong;
S-X Qiu
Jurnal Kimia Terapan Indonesia Vol 10, No 1-2 (2000)
Publisher : Research Center for Chemistry - LIPI
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DOI: 10.14203/jkti.v10i1-2.177
Ficus aurantiace a Griff (Mor aceae) collected from BukitKaba forest park, Bengkulu, was studied for the isolation andidentification of its bioactive constituents. This plant is a climbingtree, having large colored fruits, orange to red marbled withwhite spots. The methanol-soluble extract of the leaves was eluted10 silica-gel column chromatography several times using mixturesof chloroform-methanol as the elution solvent, leading to theisolation of a known compound, simiarenol. The structure ofsimiarenol was judged by its data comparison to published values,and based all the evaluation results of its physical andspectroscopic data. such as UV, IR, MS, one- and two-dimensional NMR, HMQC and HMBC.