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Jurnal Perikanan Universitas Gadjah Mada

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Published by Universitas Gadjah Mada

ISSN : 08536384 EISSN : 25025066 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Jurnal Perikanan Universitas Gadjah Mada are published to promote a critical review of the various investigative issues of interest in the field of fisheries between the researchers, academics, students and the general public, as a medium for communication, dissemination, and utilization of wider scientific activities.

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Articles 8 Documents

Search results for , issue "Vol 21, No 1 (2019)" : 8 Documents clear

Toxicity Test for Evaluating Food Safety of New Edible Seaweeds, Enteromorpha sp. and Laurencia sp. Alim Isnansetyo; Indah Istiqomah; Retno Widaningroem; Triyanto Triyanto; Rina Anggraeni Safia; Riesa Yosita; Senny Helmiati
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Seaweeds are potential foodstuff and source of bioactive substances that are very useful in the food and pharmaceutical industries. The objective of this study was to evaluate toxicity of aqueous extracts of seaweeds by Brine shrimp lethality test (BLT) and cytotoxicity test. The seaweeds used in this study were Enteromorpha sp. and Laurencia sp. collected from the Gunungkidul coastal line. The cytotoxicity test was performed against a normal cells line of Vero cells and a cancer cells line of HeLa cells. The cells lines were cultured in M119 medium with addition of 10% FBS and incubated at 37°C in CO2 incubator until confluent. Cells were harvested and cultured in 96 microplate with an initial density of 2x104 cells / 100 ml / well. The cells lines were treated with seaweed extracts at 24 hours after incubation. After additional 24 h incubation, the cell proliferation was observed by MTT method (3- [4,5-dimethylthiazol-2-yl] -2,5- diphenyltetrazolium bromide.The results showed that the two aqueous extract were not toxic in the BLT test, and did not exhibited any cytotoxicity activity against HeLa and Vero cells lines. Concentration-dependent cell proliferation was also not found. This results indicated that Enteromorpha sp. and Laurencia sp. extracts were no cytotoxic and might be consumed safely. These overall results suggested that the two seaweeds might be developed to be a new foodstuff for humans.

Turbinaria conoides Extract Increases the Storability of Red Nile Fillet at Cold Temperatures Amir Husni; Latif Sahubawa; Imam Arda Perdana
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

The aim of the research was to assess the effect of Turbinaria conoides extract as a natural antibacterial agents on shelf life of red tilapia fillet at low temperature. The fresh red tilapia fillet soaked with a Turbinaria conoides extract solution for 30 minutes at various concentration of 0.0%; 0.5%: 1.0%; 1.5% and 2.0%, than stored at low temperature (4°C) for 12 days with four days interval observation, repeteadly. The parameter observed wereTotal Plate Count (TPC), Total Volatile Base (TVB), pH and scoring. The result showed that Turbinaria conoides extract able to maintain shelf life of red tilapia fillet at low temperature up to 12 days based on the content of TPC and TVB. Turbinaria conoides extract can maintain the score of appearance and texture of red tilapia up to 8 days. The treatment 1.5% of Turbinaria conoides extract was the best treatment in maintaining shelf life of red tilapia fillet at low temperature based on the parameters of TPC, TVB, appearance and texture.

Biosorption Cr(VI) using Biofilm Streamer Andi Kurniawan
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Water contamination is one of the main environmental problem presently. One of the contaminants that become serious problem in aquatic ecosystems is Cr(VI) which is toxic for living organisms. Therefore, technology to solve this problem is urgent. Biosorption is one of the alternative technologies that inexpensive and environmentally friendly. The present study analyzed the biosorption of Cr(VI) using streamer biofilm. Biofilm is a predominant habitat for most microbes in aquatic ecosystems. The sample of biofilms used in this study was streamer biofilm collected from the river. The results show that the streamer biofilm had ability to adsorb Cr(VI). The adsorption of Cr(VI) are fitted well to the Langmuir adsorption isotherm model. The maximum adsorbed amount of Cr(VI) is estimated to be around 8.33 mg/g and the adsorption equilibrium constant is around 0.02 L/mg. The results of the present study indicate that the streamer biofilm is a promising alternative biosorbent for the biosorption of water pollutant such as Cr(VI).

Utilization of Water-Soluble Chitosan as Antiseptic Hand Sanitizer Anies Chamidah; Christina Nur Widiyanti; Nahda Nur Fabiyani
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Shrimp wastes produced by manufacturers are mostly limited to be utilized by small industries. They only can turn the waste into chips and shrimp pastes even though it can be used as chitosan. The scarcity of shrimp waste utilized as chitosan is often constrained due to the lack of chitosan dissolve in water caused by the length of the molecular chain. In order to optimize the chitosan utilization, depolymerization is used; such as using H2O2. Chitosan depolymerization enables chitosan to be water soluble and has a high antibacterial ability, which can be applied as an antiseptic of hand sanitizer which has been derived from synthetic materials. The used method is an experimental method which includes the processing of making water-soluble chitosan with test parameters of deacetylation, solubility, moisture content, and yield. Furthermore, the manufacture of hand sanitizers is carried out in vitro and in vivo. The results showed that water-soluble chitosan can be made at 13% H2O2 concentration at 40°C with deacetylation degree 94.21%, 90% solubility, 10.60% moisture content and 3.5% yield. Moreover, after becoming a hand sanitizer, it was able to inhibit Escherichia coli and Staphylococcus epidermidis bacteria at concentrations of 120 mg/ml, respectively 19.53 mm and 21.12 mm, which were relatively strong. Resulted in MIC values 0.28% and 0.27%, and MBC values 1.12% and 1.08%, and also without causing irritation or edema on mouse skin.

Characteristics of Thermal Front in the Tropical Waters of Eastern Indian Ocean Soni Rohima Daulay; Tengku Ersti Yulika Sari; Usman Usman; Romie Jhonnerie
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

This study aims to elucidate spatio-temporal variability of the thermal front in the eastern tropical Indian Ocean of the western Sumatera. The research was conducted during November 2017- February 2018. The Single Image Edge Detection (SIED) was applied to daily sea surface temperature (SST) data of 2016 of the level-3 Aqua Moderate Resolution Imaging Spectroradiometer (MODIS) for the detection of thermal front. The number of the thermal front events during 2016 were 167 days. The distribution and frequency of thermal front mostly found in January, i.e. 23 days with SST mean of 30.3ºC. Whereas the lowest distribution appeared in November and the lowest frequency observed in September, i.e. 6 days with the SST mean of 29.1ºC. The highest temperature range of thermal front is between 31.4-32.0ºC and the lowest ranged between 26.4-29.3ºC. The occurrences of thermal front were commonly found in the open ocean. The highest frequency occurred in January and the lowest took place in September with the longest duration of 3 days.

Isolation, Characterization and Pathogenicity of Edwardsiella tarda a Causative Disease on Freshwater Fish in Yogyakarta Murwantoko Murwantoko; Eka Diniarti; Triyanto Triyanto
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Edwarsiella tarda is a cosmopolitan bacterium and is a cause of Edwardsiellosis in various fish species. The bacterial infection causes large losses on aquaculture in Asia, especially Japan. This study was conducted to isolate and characterize E. tarda as causative disease in freshwater fishes, and to determine its pathogenicity to catfish (Pangasius sp.). Bacteria were isolated from kidney of diseased fishes on Tryptone Soya Agar medium. Identification was conducted based on morphological colonies, morphological cells and biochemical tests. Fulfillment of Koch Postulates was done by injecting bacteria intraperitoneally on 7-9 cm fishes at dose of 107 cfu/fish. Pathogenicity test was carried out by intraperitoneal injection at 104, 105, 106, and 107 cfu/fish to 7-9 cm-catfish (Pangasius sp.) and followed by observation of disease signs and mortality every six hours for 7 days. Pathogenicity was determined as Lethal Dosage (LD50) using Dragstedt Behrens method. In this research we have isolated three isolates E. tarda causing disease in fishes. The clinical signs of this disease were lose of pigmentation over the lession, swollen of stomach, haemorhage on fins , small cutaneous lesions, and necrotic on fins area. The LD50 of E. tarda isolate L2, L3, and N3 were 4.64 ± 0.35x105, 1.54 ± 0.07x105, and 1.13 ± 0.13x106 cfu/fish, respectively.

The Effect of Gelatin from Different Fish Skin on Physical and Sensory Characteristics of Marsmallow Izmy Nur Aziza; Yudhomenggolo Sastro Darmanto; Retno Ayu Kurniasih
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Gelatin is one type of protein obtained from partial hydrolysis of natural collagen. Utilization of gelatin has been widely applied to food, especially in foods related to elastic texture, for example marshmallow. The purpose of this research was to determine the quality of various types of gelatin based on gel strength, elsticity, water content, hedonic and to know the best marshmallow. The experimental design was complete randomized design. The treatment was different fish skin gelatin, namely patin, payus, and cobia with concentration of 7.39%. The parameters consisted of gelatin tests (yield, gel strength, viscosity) and marshmallow tests (gel strength, elasticity, water content and hedonic). Parametric data were analyzed with Analysis of Varience test and continued with Honestly Significant Difference test. The results showed that the use of different skin had significantly different (P<0.05) on yield, gel strength and viscosity, with the result of patin (13.24%, 280.56 bloom, 2.05 cP), payus (15.47%, 328 bloom, 3.18 cP), cobia 17.88%, 392.24 bloom, 5.63, respectively. The use of gelatin from payus and cobia had significantly different (P<0.05) to marshmallow characteristic. Marshmallow with gelatin skin of cobia fish had gel strength 1564.19 g.cm, elasticity 95.16 mm, water content 14.41% and hedonic test with interval of of 7.52 < μ < 7.91 which was means liked by panelists.

Seahorse Hydrolisate (Hippocampus kuda) and Anti-Inflammatory Activity Test with Protein Denaturation Inhibition Method Nur Azizah Nasution; Mala Nurilmala; Asadatun Abdullah
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Seahorse is marine biota that have commercial value and used as ornamental fish and traditional medicinal ingredients. Utilization of seahorses has still not been extensively studied, especially in the form of hydrolysates. Hydrolysates of seahorses are known to have efficacy in biological activity. This study was conducted to identify seahorse morphometrics, determine selected enzymes based on the degree of hydrolysis and determine the anti-inflammatory activity of hydrolysates. The results showed that morphometric identification showed morphological characteristics of Hippocampus kuda, namely the number of body rings 12 units, tail ring 35 units, eye protrusion and cheeks as much as 1 unit, average head length of 3.5 cm, number of body rings on dorsal fin 3 units. The best degree of hydrolysis of seahorses is protein hydrolysis using alkalase enzyme of 42.49%. The highest anti-inflammatory activity on sea horse hydrolyzate has %inhibition of 42.88%.

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